Article
Clinical Neurology
Yuki Minegishi, Junji Nishimoto, Minori Uto, Kaichi Ozone, Yuichiro Oka, Takanori Kokubun, Kenji Murata, Hidenori Takemoto, Naohiko Kanemura
Summary: Motor function recovery is often poor after peripheral nerve injury. The effect of different numbers of nerve crushes on muscle reinnervation and spinal circuit plasticity is varied. However, exercise intervention may improve motor function recovery regardless of the number of crushes.
Article
Neurosciences
Kirkwood E. E. Personius, Danielle Siebert, Dennis W. W. Koch, Susan B. B. Udin
Summary: Motor axons in peripheral nerves can regenerate after injury, but complete recovery of motor function is rare, especially when reinnervation is delayed. Glutamate receptors have been found to play a crucial role in the innervation of muscle during development. Blocking NMDA receptor activity delays the elimination of excessive connections. This study investigates whether glutamate receptors have a similar role in the transition from polyneuronal to mono-innervation and in the recovery of function after peripheral nerve injury in mature muscle.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Cell Biology
Bruno Henrique de Melo Lima, Andre Luis Bombeiro, Luciana Politti Cartarozzi, Alexandre Leite Rodrigues De Oliveira
Summary: The expression of MHC-I and other related molecules following peripheral nerve injury is associated with the recovery of neurons, glial cells, sensorimotor function, gait, and nociceptive threshold in mice. Different strains of mice exhibit varying recovery capacities.
Article
Environmental Sciences
Bin Bin Wang, Chao Guo, Sheng Qiao Sun, Xing Nan Zhang, Zhen Li, Wei Jie Li, De Zhi Li, Michael Schumacher, Song Liu
Summary: This study compared the effect of different peripheral nerve injury models on nerve regeneration through animal experiments. It was found that the regeneration speed was faster in the crush injury model, while it was slower in the transection injury model. This provides some reference for the selection of clinical research models.
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
(2023)
Article
Engineering, Biomedical
Binghui Jin, Yunru Yu, Xiangxiang Chen, Yanhong Yang, Yushan Xiong, Young Jun Im, Yuanjin Zhao, Jian Xiao
Summary: The study proposes a novel microtube for sciatic nerve regeneration using gradient decellularized porcine sciatic nerve extracellular matrix hydrogel (pDScNM-gel). The pDScNM is shown to enhance cell proliferation and migration, improve axon growth, and these effects can also be achieved through coculture in a gradient pDScNM microtube. In vivo sciatic nerve regeneration and functional recovery are demonstrated by assembling the gradient pDScNM microtubes with a medical silicon tube.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Muhammad Imran, Ghulam Hussain, Arruje Hameed, Iqra Iftikhar, Muhammad Ibrahim, Rahat Asghar, Izzat Nisar, Tahir Farooq, Tanzila Khalid, Kanwal Rehman, Mohammed A. Assiri
Summary: This study screened the functional metabolites of Moringa oleifera and found that they play an important role in accelerated functional recovery after peripheral nerve injury. The extracts from Moringa showed potential in promoting the recovery of sensory and motor functions, as well as controlling oxidative stress and facilitating nerve regeneration. This study highlights the significance of Moringa as a sustainable source of nutritive metabolites and a valuable target for drug development.
Article
Biochemical Research Methods
Wen-Yo Tu, Wentao Xu, Kejing Zhang, Chengyong Shen
Summary: The study focuses on a simple method for whole-mount immunostaining of the adult diaphragm in mouse models, allowing researchers to perform a detailed analysis of the neuromuscular system. By using Perspex boards to thin and spread the diaphragm, a clear innervation pattern can be observed, increasing the reliability of NMJ phenotype assessment.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Article
Biochemistry & Molecular Biology
Svetlana Demyanenko, Maria A. Pitinova, Yulia N. Kalyuzhnaya, Andrey M. Khaitin, Svetlana A. Batalshchikova, Natalya M. Dobaeva, Yulia A. Shevtsova, Kirill Goryunov, Egor Y. Plotnikov, Svetlana G. Pashkevich, Gennady T. Sukhikh, Denis N. Silachev
Summary: Peripheral nerve injury is a significant medical problem without effective treatment currently. However, the use of multipotent mesenchymal cells (MSCs)-derived extracellular vesicles (EVs) shows promise in promoting the regeneration of peripheral nerves after injury. In a rat model of sciatic nerve injury, the application of MSCs-EVs gel significantly reduced muscle atrophy and improved functional recovery. The results suggest that this therapeutic approach may be efficient for the treatment of acute peripheral nervous system injuries.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Neelam Ahuja, Kamal Awad, Sara Peper, Marco Brotto, Venu Varanasi
Summary: Volumetric muscle loss (VML) exceeds the intrinsic regenerative capacity of muscle tissue and requires intervention. Combined treatment for neuromuscular junction (NMJ) and muscle regeneration is crucial for repair and regeneration.
NEUROSCIENCE LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Giorgia D'Este, Marco Stazi, Samuele Negro, Aram Megighian, Florigio Lista, Ornella Rossetto, Cesare Montecucco, Michela Rigoni, Marco Pirazzini
Summary: In this study, the main neurotoxic component of black widow spider venom, alpha-Latrotoxin, was used as a molecular tool to identify factors contributing to the recovery of the neuromuscular junction (NMJ). The study found that Urocortin 2 (UCN2), a neuropeptide involved in stress response, is rapidly expressed at the NMJ after damage, and its specific receptor CRHR2 plays a crucial role in the rescue of neuromuscular transmission. Furthermore, the study revealed that exogenous administration of UCN2 accelerates the regrowth of motor axon terminals and enhances synaptic maturation, thereby facilitating the functional recovery of neuromuscular transmission after damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Qunzhou Zhang, Justin C. Burrell, Jincheng Zeng, Faizan Motiwala, Shihong Shi, D. Kacy Cullen, Anh D. Le
Summary: This study generated gingiva-derived mesenchymal stem cell (GMSC)-repopulated nerve protectors and evaluated their therapeutic effects in a crush injury model. The results showed that the Schwann-like cells converted from GMSCs were able to promote functional recovery and axonal regeneration of injured sciatic nerves.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Neurosciences
Leyang Li, Su Chen, Hiroyuki Yokoyama, Hidetoshi Kaburagi, Takashi Hirai, Kunikazu Tsuji, Mitsuhiro Enomoto, Yoshiaki Wakabayashi, Atsushi Okawa
Summary: Peripheral nerve injury (PNI) leads to severe functional loss in extremities, and delayed nerve repair results in muscle denervation and atrophy. To better understand NMJ degeneration and regeneration after PNI, we established two models of nerve repair in mice and compared their outcomes. Our results suggest that allogeneic nerve grafting is more effective than end-to-end neurorrhaphy in promoting functional recovery and increasing reinnervated NMJs and Schwann cells in the target muscle. Further investigation is needed to explore the relationship between NMJ and Schwann cells in the target muscle.
Article
Engineering, Biomedical
Luana Gabriel de Souza, Ketlyn Germann Hendler, Alexandre Marcio Marcolino, Heloyse Uliam Kuriki, Ramon Bauer Cardoso, Marisa de Cassia Registro Fonseca, Rafael Inacio Barbosa
Summary: The study showed that PBM alone was more effective compared to Simvastatin alone or the combination of PBM and Simvastatin for sciatic nerve injury in mice.
LASERS IN MEDICAL SCIENCE
(2021)
Article
Orthopedics
Chen Yu, Xiaoxu Wang, Jian Qin
Summary: Necrostatin-1 (Nec-1) can reduce peripheral nerve lesions and protect Schwann cells and axons by inhibiting necroptosis. It also reduces inflammation and oxidative stress by inhibiting RIP1.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Review
Neurosciences
Rachel Rios, Albina Jablonka-Shariff, Curtis Broberg, Alison K. Snyder-Warwick
Summary: Macrophages play crucial roles in repairing peripheral nerve injuries and promoting NMJ reinnervation, suggesting their potential in facilitating motor recovery.
MOLECULAR AND CELLULAR NEUROSCIENCE
(2021)