Article
Chemistry, Multidisciplinary
Ariel A. Szklanny, Majd Machour, Idan Redenski, Vaclav Chochola, Idit Goldfracht, Ben Kaplan, Mark Epshtein, Haneen Simaan Yameen, Uri Merdler, Adam Feinberg, Dror Seliktar, Netanel Korin, Josef Jaros, Shulamit Levenberg
Summary: Creating engineered hierarchical vasculatures is crucial for implantable functional thick tissues. This study introduces a combined approach using millimetric vessel-like scaffolds and 3D bioprinted vascularized tissues to achieve fully engineered hierarchical vascular constructs for implantation. The use of sacrificial molds, endothelial cells, and vascularizing hydrogels enables the formation of functional vessels within the engineered tissue, promoting tissue perfusion and host vasculature ingrowth.
ADVANCED MATERIALS
(2021)
Article
Oncology
Rachel Kerslake, Birhanu Belay, Suzana Panfilov, Marcia Hall, Ioannis Kyrou, Harpal S. S. Randeva, Jari Hyttinen, Emmanouil Karteris, Cristina Sisu
Summary: This study evaluates the impact of growth conditions on cancer cells and compares their behavior to traditional two-dimensional models using transcriptomics, clinical, and novel experimental data. The results show that variability in growth conditions can affect key cancer genes and biological processes. It highlights the need for future studies to identify the most suitable in vitro/preclinical model for studying tumor microenvironments.
Article
Cell Biology
Ewa Mazurkiewicz, Aleksandra Makowiecka, Ewa Mrowczynska, Iryna Kopernyk, Dorota Nowak, Antonina Joanna Mazur
Summary: Skin melanocytes need to cross the basement membrane (BM) to invade into the skin, and changes in the BM composition are linked to melanocytes tumorigenesis. The study demonstrates the significance of gelsolin (GSN) for melanoma cell migration, especially on laminin, a key component of the skin's BM.
Article
Biochemistry & Molecular Biology
Young Joon Suh, Mrinal Pandey, Jeffrey E. Segall, Mingming Wu
Summary: Epidermal growth factor (EGF) promotes tumor invasion. This study found that tumor cell clusters respond differently to EGF gradients compared to single cells, showing enhanced motility and mild chemotaxis. These findings highlight the importance of tumor spheroid platforms for studying tumor invasion.
Article
Integrative & Complementary Medicine
Han Chun-hui, Ma Jing-yun, Zou Wei, Qu Jia-lin, Du Yang, Li Na, Liu Yong, Jin Guo, Leng Ai-jing, Liu Jing
Summary: This study investigated the anti-invasion efficacy of the ethanol extract of Oldenlandia diffusa Will. (EEOD) on human malignant glioma (MG) cell invasion using a microfluidic chip culture model. The results showed that EEOD suppressed MG cell viability, promoted apoptosis, and inhibited migratory and invasive abilities. Network pharmacology analysis revealed that the anti-invasion effect of EEOD might be mediated through the regulation of MAPK and Wnt signaling pathways and microtubule cytoskeleton organization.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Rajesh M. Jagirdar, Eleftherios D. Papazoglou, Eleanna Pitaraki, Olympia A. Kouliou, Erasmia Rouka, Lydia Giannakou, Stefanos Giannopoulos, Sotirios Sinis, Chrissi Hatzoglou, Konstantinos Gourgoulianis, Sotirios G. Zarogiannis
Summary: The study found that fibronectin and homologous cell-derived extracellular matrix affect malignant cell behavior differently, with higher 3D cell spheroid invasion in fibronectin-collagen matrices and higher contractility in epithelioid and biphasic MPM cells in fibronectin-collagen matrices compared to homologous cell-derived extracellular matrix-collagen. Cell aggregates also showed invasive behavior in homologous cell-derived extracellular matrix alone. This suggests that ECM and dimensionality play a role in affecting malignant cell behavior during cell culture studies.
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2021)
Article
Cell Biology
Naagarajan Narayanan, Sarah Calve
Summary: The muscle-tendon interface is a crucial anatomical region that plays a significant role in efficient force transmission and injury prevention. Understanding the role of the extracellular matrix (ECM) in muscle and tendon tissues has the potential to accelerate repair processes and develop regenerative medicine strategies. Advanced techniques for exploring the ECM, coupled with regenerative medicine advancements, offer promising avenues for next-generation therapies at the muscle-tendon interface.
CONNECTIVE TISSUE RESEARCH
(2021)
Meeting Abstract
Oncology
Heather J. McKinnon, Joanna Birch, Laura McDonald, Mairi Sime, Daniel Croft, Diane Crighton, Martin Drysdale, Lesley Gilmore, Christopher Gray, Jennifer Konczal, Duncan McArthur, Patricia McConnell, Mokdad Mezna, Alexander Schuettelkopf, Karen Strathdee, Justin Bower, Michael F. Olson, Anthony J. Chalmers
Article
Multidisciplinary Sciences
Ya Hua Chim, Louise M. Mason, Nicola Rath, Michael F. Olson, Manlio Tassieri, Huabing Yin
SCIENTIFIC REPORTS
(2018)
Article
Cell Biology
Dominika A. Rudzka, Giulia Spennati, David J. McGarry, Ya-Hua Chim, Matthew Neilson, Aleksandra Ptak, June Munro, Gabriela Kalna, Ann Hedley, Daniela Moralli, Catherine Green, Susan Mason, Karen Blyth, Margaret Mullin, Huabing Yin, Michael F. Olson
JOURNAL OF CELL SCIENCE
(2019)
Article
Oncology
Giulia Spennati, Lisa F. Horowitz, David J. McGarry, Dominika A. Rudzka, Garett Armstrong, Michael F. Olson, Albert Folch, Huabing Yin
Summary: Metastasis is the primary cause of cancer patient mortality, and studying this process using organotypic liver and brain slices shows promise in closely replicating the tumour microenvironment for in vitro testing. Different invasion patterns and behaviors were observed in the breast cancer cells within the brain and liver slices, with variations in cell stiffness and adhesion forces influencing invasiveness. Inhibition of the Ras/MAPK/ERK pathway resulted in reduced invasiveness, highlighting the potential of organotypic tissue slices to better mimic in vivo conditions during cancer cell metastasis compared to traditional in vitro models.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biology
Linda Julian, Gregory Naylor, Grant R. Wickman, Nicola Rath, Giovanni Castino, David Stevenson, Sheila Bryson, June Munro, Lynn McGarry, Margaret Mullin, Alistair Rice, Armandodel Del Rio Hernandez, Michael F. Olson
Summary: The activation of ROCK1 and consequent cell contraction are necessary to limit sterile inflammation and damage amplification following tissue-scale cell death. Inhibition of HMGB1 can reduce liver damage and neutrophil infiltration, while its inhibition can increase hepatocellular carcinoma development. Acute sterile inflammation also serves as an efficient tumor-suppressive mechanism.
Editorial Material
Cell Biology
Michael F. Olson, Laura M. Machesky
Summary: The study demonstrates that MICAL2 mediates methionine oxidation of ARP3B, disrupting ARP2/3 complexes and causing disassembly of branched actin filaments.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Mackenzie Hurst, David J. McGarry, Michael F. Olson
Summary: Rho GTPases play crucial roles in regulating the actomyosin cytoskeleton and various cellular processes. While the GTPase cycle is the main method of regulation, post-translational modifications, particularly protein oxidation, also play important roles. Further research is needed to fully understand how lipidation, phosphorylation, and oxidation affect the regulation and function of Rho GTPases.
Article
Oncology
David J. McGarry, Garett Armstrong, Giovanni Castino, Susan Mason, William Clark, Robin Shaw, Lynn McGarry, Karen Blyth, Michael F. Olson
Summary: The disruption of MICAL1 gene affected F-actin organization, cell size, motility and gene expression, with over 700 genes showing significant changes. Receptor regulator activity was identified as the most significant negatively enriched molecular function gene set, and MICAL1 disruption attenuated breast cancer tumor growth in vivo. Elevated MICAL1 gene expression was associated with invasive breast cancer and reduced progression free survival in human patients.
Review
Genetics & Heredity
Sipan Haikazian, Michael F. Olson
Summary: This article discusses the mutations in the LGI1, RELN, and MICAL1 genes associated with autosomal dominant lateral temporal epilepsy (ADLTE), with a particular focus on the G150S point mutation found in MICAL1. Additionally, the article explores the potential link between these activating MICAL1 mutations and cancer.
Article
Oncology
Federica Tonon, Maja Cemazar, Urska Kamensek, Cristina Zennaro, Gabriele Pozzato, Sergio Caserta, Flora Ascione, Mario Grassi, Stefano Guido, Cinzia Ferrari, Laura Cansolino, Francesco Trotta, Biljana Grcar Kuzmanov, Giancarlo Forte, Fabiana Martino, Francesca Perrone, Riccardo Bomben, Valter Gattei, Nicola Elvassore, Erminio Murano, Nhung Hai Truong, Michael Olson, Rossella Farra, Gabriele Grassi, Barbara Dapas
Summary: The study demonstrates that 5-Aza can impair the development of HCC by upregulating miR-139-5p, which then affects the proliferative and migratory pathways of HCC.
Article
Cell Biology
David J. McGarry, Giovanni Castino, Sergio Lilla, Alexandre Carnet, Loughlin Kelly, Katarina Micovic, Sara Zanivaran, Michael F. Olson
Summary: The MICAL1 monooxygenase is regulated by CDC42 GTPase effector PAK1, which phosphorylates MICAL1 and accelerates F-actin disassembly. External signals can lead to PAK-dependent phosphorylation of MICAL1, linking extracellular signals to cytoskeleton regulation.
Article
Oncology
Gregory Naylor, Linda Julian, Steven Watson-Bryce, Margaret Mullin, Robert J. Nibbs, Michael F. Olson
Summary: This article discusses the therapeutic effects of ROCK inhibitors on tumors and finds that using ROCK inhibitors to induce immunogenic cell death (ICD) may not increase therapeutic efficacy compared to standard care therapies.
Editorial Material
Cell Biology
Michael F. F. Olson
Review
Cell Biology
Vanessa M. Ruscetta, Taj J. J. Seaton, Aleen Shakeel, Stanley N. S. Vasconcelos, Russell D. D. Viirre, Marc J. J. Adler, Michael F. F. Olson
Summary: Cytoskeleton organization and dynamics are regulated by post-translational modifications of key target proteins, including the phosphorylation of myosin light chain proteins by MRCK kinases. Compared to ROCK kinases, the contributions of MRCK kinases are less characterized due to the late discovery of selective inhibitors. The recent development of selective MRCK inhibitors has expanded the tools to study MRCK function and shown therapeutic benefits in cancer studies.
Editorial Material
Cell Biology
Takaya Totsuka, Takashi Akera, Michael F. Olson
Summary: During the second meiotic cell division, MRCK beta kinase is identified as an essential kinase for efficient spindle rotation. It activates cortical myosin II rings to prevent the sticky interaction between the cell cortex and chromatin, facilitating spindle rotation. The same MRCK-myosin II pathway operates in zygotes to promote parental genome unification.
JOURNAL OF CELL BIOLOGY
(2023)