Article
Plant Sciences
Taras Pasternak, Ivan A. Paponov, Serhii Kondratenko
Summary: Procedures have been optimized for direct regeneration of entire plants from shoot and root protoplasts of Arabidopsis thaliana, including adjustments to culture media, detection protocols, and optimization of cell reprogramming. Cell division competence was detected through DNA replication events and high cell density, and further optimized by applying glutathione to increase sensitivity to cell cycle activation by auxin. The developed protocol allows investigation into the molecular mechanism of somatic plant cell de-differentiation.
Article
Plant Sciences
Ingrid Berenice Sanchez Carrillo, Patrick C. Hoffmann, Teura Barff, Martin Beck, Hugo Germain
Summary: This study describes a workflow for preparing cryo-ET of root protoplasts from Arabidopsis thaliana plants. The method provides a novel application for studying various characteristics of plant cells.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Shunji Nakamura, Tokitaka Oyama
Summary: The plant circadian system is composed of cellular oscillations that help plants adapt to daily and seasonal environmental changes. In this study, the circadian properties of individual cells derived from leaves and roots of an Arabidopsis plant were analyzed. It was found that individual cells without physical contact with other cells possess a genuine circadian clock, with a period similar to 24 hours. The stability of the circadian rhythm was influenced by cell density, and quantitative and statistical analyses showed differences in light/dark entrainment between leaf-derived and root-derived cells, indicating diversification of the circadian system at the cellular level in different plant organs.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Hiroyuki Ishida, Yu Okashita, Hiromi Ishida, Makoto Hayashi, Masanori Izumi, Amane Makino, Nazmul H. Bhuiyan, Klaas J. van Wijk
Summary: Chloroplasts, and plastids in general, are major sources of carbon and nitrogen recycling, being partially degraded by piecemeal autophagy. A mutant, gfs9-5, with defective membrane-trafficking factor GFS9, accumulates plastid bodies (PBs) and shows increased autophagic flux, suggesting a potential connection between GFS9 and autophagy in the process of piecemeal plastid autophagy.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Plant Sciences
Phan Phuong Thao Doan, Jin Hee Kim, Jeongsik Kim
Summary: Leaf senescence is regulated by a complex network of genes. In this study, the researchers developed a cellular approach using transient expression in Arabidopsis protoplasts to examine the functional roles of senescence regulatory genes. They validated this system and found that it can effectively monitor the expression of senescence-responsive genes. The results provided molecular evidence for the distinct kinetic involvement of these genes in leaf senescence regulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Analytical
Shashini De Silva, Iran Ocana-Rios, Cecilia Cagliero, Morgan R. Gostel, Gabriel Johnson, Jared L. Anderson
Summary: A simple and rapid ionic liquid-based extraction method was developed for isolating genomic DNA from Arabidopsis thaliana plant tissue. The method involved using ionic liquid or magnetic ionic liquid directly applied to treated plant tissue to facilitate cell lysis and DNA extraction. The DNA-enriched extracts could be directly used in qPCR without needing additional purification steps and showed good preservation of plant DNA at room temperature.
ANALYTICA CHIMICA ACTA
(2023)
Article
Plant Sciences
Jiawei Xu, Lijuan Han, Shuyan Xia, Ruojia Zhu, Erfang Kang, Zhonglin Shang
Summary: It has been found that AtANN3, a protein involved in plant cellular metabolism, plays a crucial role in the regulation of root and hypocotyl growth by extracellular ATP (eATP). In the AtANN3 null mutants, the inhibitory effects of eATP on root and hypocotyl elongation were weakened. Additionally, the eATP-induced asymmetric distribution of auxin and auxin transporters in root and hypocotyl cells was impaired in these mutants. These findings suggest that AtANN3 is involved in eATP-regulated seedling growth by regulating the distribution of auxin and auxin transporters.
Article
Multidisciplinary Sciences
Akiko Watanabe, Yoshino Hatanaka, Yukino Takeshima, Karin Sasaki, Noa Takahashi, Yukihiro Ito
Summary: This study demonstrates that rice seedlings grown in darkness can produce a comparable amount of soluble protein to those grown in light. Optimal conditions for protein production in dark-grown rice seedlings include large seeds, germination and growth at 28 degrees C, supplementation with specific medium and sucrose, and dense planting. Therefore, this research suggests the possibility of producing foreign proteins in rice seedlings in the dark, reducing electricity usage and increasing cost-effectiveness.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Sabrina Flutsch, Daniel Horrer, Diana Santelia
Summary: In this study, we characterized the starch synthesis dynamics in guard cells (GCs) and discovered that GCs have metabolic features of both photoautotrophic and heterotrophic cells. We found that GCs use different carbon precursors to synthesize starch depending on the time of day, which can come from both GC photosynthesis and sugars imported from the leaf mesophyll. We also identified the major enzymes involved in GC starch synthesis and demonstrated their temporal activation according to the fluctuations of stomatal aperture.
Article
Plant Sciences
Daniele Y. Sunaga-Franze, Jose M. Muino, Caroline Braeuning, Xiaocai Xu, Minglei Zong, Cezary Smaczniak, Wenhao Yan, Cornelius Fischer, Ramon Vidal, Magdalena Kliem, Kerstin Kaufmann, Sascha Sauer
Summary: The researchers have introduced a method for plant nucleus isolation that can be used for single-cell genomics research, demonstrating its applicability in different plant species and tissues. By analyzing the transcriptomes of Arabidopsis thaliana seedlings and developing flowers, the potential of this method in exploring plant development and gene regulation processes was revealed.
Article
Horticulture
Yingdan Yuan, Mengting Zu, Lanping Sun, Jiajia Zuo, Jun Tao
Summary: Plant growth-promoting rhizobacteria (PGPR) strains were isolated from the rhizosphere soil of Paeonia lactiflora cultivars and found to have diverse growth-promoting abilities and mechanisms. Inoculating with these strains significantly enhanced the growth and development of Paeonia lactiflora and Arabidopsis thaliana.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Jun Murata, Takehiro Watanabe, Hajime Komura
Summary: Research has found that volatile organic compounds released by soil microbes can affect the growth of Arabidopsis through interkingdom signals. Isovaleric acid has been identified as a compound with inhibitory growth activity towards Arabidopsis seedlings.
Article
Ecology
Jose Lopez-Hernandez, Elizabeth Garcia-Cardenas, Jesus Salvador Lopez-Bucio, Kiran Rubi Jimenez-Vazquez, Homero Reyes de la Cruz, Ofelia Ferrera-Rodriguez, Dulce Lizbeth Santos-Rodriguez, Randy Ortiz-Castro, Jose Lopez-Bucio
Summary: The interaction between plants and bacteria plays a crucial role in their adaptation to challenging environments, involving nutrient solubilization, root restructuring, and modulation of hormonal responses. This study isolated and characterized bacteria strains with high capability in solubilizing calcium phosphate, identifying their varying effects on the growth of Arabidopsis seedlings. Among the strains, Pseudomonas brassicae showed the most significant promotion of plant growth.
Article
Biochemical Research Methods
L. Vanderstraeten, R. Sanchez-Munoz, T. Depaepe, F. Auwelaert, D. Van Der Straeten
Summary: We present an optimized protocol for hypocotyl grafting of 4-day-old Arabidopsis thaliana seedlings. The combination of conditions yields a grafting success of 90-100% and provides an easy and accessible methodology, reducing the time frame, and without the necessity of acquiring specialized equipment. The presented protocol is simple, fast, and highly efficient, easing the inclusion of hypocotyl grafting assays in any research project. In addition, the description of the protocol is detailed to a level ensuring that even non-trained researchers are sufficiently prepared to adopt the grafting methodology.
Article
Biochemistry & Molecular Biology
Peilin Wang, Yuanchun Pu, Muhammad Ali Abid, Linglin Kang, Yulu Ye, Man Zhang, Chengzhen Liang, Yunxiao Wei, Rui Zhang, Zhigang Meng
Summary: Protoplast extraction and transformation techniques for cotton were efficiently studied in this research. By optimizing the ratio of enzymes, the quantity and activity of extracted protoplasts were significantly increased, and fluorescent protoplasts were successfully obtained. The study also revealed the importance of plasmid concentration and transformation time on transformation efficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)