Article
Multidisciplinary Sciences
Takumi Hiyoshi, Hisanori Domon, Tomoki Maekawa, Hikaru Tamura, Toshihito Isono, Satoru Hirayama, Karin Sasagawa, Fumio Takizawa, Koichi Tabeta, Yutaka Terao
Summary: This study reveals the mechanisms by which neutrophil elastase (NE) exacerbates periodontitis. NE induces increased neutrophil recruitment and activity in the periodontal tissue, leading to worsened periodontal bone loss. Administration of an NE inhibitor reduces NE activity and attenuates periodontal bone loss. NE also promotes bacterial invasion in periodontal tissues by cleaving cell adhesion molecules and disrupting the gingival epithelial barrier.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Javier Fernandez, Constanza Jimenez, Dafna Benadof, Paulina Morales, Jessica Astorga, Felipe Caceres, Marcela Hernandez, Alejandra Fernandez, Fernando Valenzuela
Summary: The study aimed to evaluate the levels of MMP-2 and MMP-9 in gingival crevicular fluid as novel biomarkers for rosacea. The results showed a significant increase in MMP-9 concentration in the GCF of patients with rosacea compared to healthy patients. MMP-9 levels can be used as a diagnostic biomarker for rosacea.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Luigi Nibali, Abish Stephen, Eleni Hagi-Pavli, Robert Allaker, Antonino Di Pino, Valentina Terranova, Marcella Pisano, Salvatore Di Marca, Viviana Ferrara, Roberto Scicali, Mauro Giordano, Francesco Purrello, Nikolaos Donos, Lorenzo Malatino
Summary: The study suggests that GCF markers in patients with metabolic syndrome may be associated with concomitant periodontitis, particularly the levels of markers such as Aggrecan, IL-6, and IL-8 are correlated with the severity of the disease.
JOURNAL OF DENTISTRY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Bardia Vadiati Saberi, Farouq Yousefi, Tooba Yousefi, Durdi Qujeq
Summary: This study determined the correlation between mucin and alpha-amylase protein levels in gingival crevicular fluid (GCF) with chronic periodontitis. The results showed that the levels of both markers were higher in patients with chronic periodontitis compared to successfully recovered patients, suggesting that they could be used to determine the activity of periodontal disease.
Article
Health Care Sciences & Services
Hammam Ibrahim Fageeh, Hytham N. Fageeh, Shankargouda Patil
Summary: Periodontitis is an inflammatory condition caused by pathogenic bacteria in dental plaque. Macrophages derived from monocytes play a dual role in periodontitis, presenting antigens to lymphocytes while also contributing to periodontal destruction. The study found that GCF samples from periodontitis patients have higher levels of specific cytokines, and that treatment with these samples can induce monocytes to differentiate into destructive macrophages, affecting the phenotype and function of resident cells in the periodontium.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Health Care Sciences & Services
Rasha Salah, Hayder Raad Abdulbaqi
Summary: This pilot study aimed to assess the effect of oral rinsing with a combination of miswak and green tea extracts on gingival crevicular fluid (GCF) flow and IL-1 beta levels. The results showed that short-term oral rinsing with this combination did not induce significant changes in GCF flow and IL-1 beta concentration and might help retard inflammation.
Article
Dentistry, Oral Surgery & Medicine
Panagiota Katsiki, Kamran Nazmi, Bruno G. Loos, Marja L. Laine, Kim Schaap, Esen Hepdenizli, Floris J. Bikker, Henk S. Brand, Enno C. I. Veerman, Elena A. Nicu
Summary: The study aimed to explore the feasibility of screening for periodontitis by measuring biomarkers in saliva, oral rinse, and gingival crevicular fluid. Results showed that the combination of MMP-8 and chitinase in oral rinse samples can accurately discriminate between periodontitis and periodontal health/gingivitis, with MMP-8 and TPA concentrations significantly higher in periodontitis patients.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2021)
Article
Biochemical Research Methods
Xin Zhang, Xiaoping Xiao, Yue Mu, Yao Ran Liu, Xinxin Lin, Qian Li, Wei Sun
Summary: This study investigated the influence of tooth site, sex, and age on the normal GCF proteome in periodontal health. Results showed that tooth site had a small impact, while sex and age were important factors affecting the GCF proteome, with sex having a significant influence on young adults.
JOURNAL OF PROTEOMICS
(2022)
Article
Biology
Hytham N. Fageeh, Hammam Ibrahim Fageeh, Samar Saeed Khan, Prabhadevi C. Maganur, Nishant Vyas, Vikrant R. Patil, A. Thirumal Raj, Hosam Ali Baeshen, Shankargouda Patil
Summary: This study aimed to characterize monocytes isolated from the gingival crevicular fluid (GCF) in patients with periodontitis, evaluating cytokine levels, gene expression, and secreted cytokines and chemokines. CD14+ monocytes from GCF secreted higher levels of CCL2 and expressed genes responsible for monocyte migration, indicating their role in the inflammatory microenvironment of periodontitis.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Zeliha Guney, Sivge Kurgan, Canan Onder, Cavid Mammadov, Muhittin A. Serdar, Meral Gunhan
Summary: This study aimed to evaluate the levels of ADMA, SDMA, and IL-1 beta in GCF from periodontitis patients. The results showed that the levels of IL-1 beta, ADMA, and SDMA in GCF were significantly higher in periodontitis patients compared to the control group, and they were positively correlated with clinical periodontal parameters. It suggests that ADMA and SDMA may be involved in the pathogenesis of periodontal disease.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Microbiology
Simran Preet Bhalla, Ann Maria Shaju, Carlos Marcelo da Silva Figueredo, Leticia Algarves Miranda
Summary: C5a levels were significantly increased in both gingival crevicular fluid and saliva of patients with periodontal disease, indicating a potential role of this molecule in the pathogenesis of the disease.
Article
Biology
Ashley L. L. Gard, Rebeccah J. J. Luu, Ryan Maloney, Madeline H. H. Cooper, Brian P. P. Cain, Hesham Azizgolshani, Brett C. C. Isenberg, Jeffrey T. T. Borenstein, Jane Ong, Joseph L. L. Charest, Else M. M. Vedula
Summary: A microfluidic model of human gingival tissue allows for the study of disease progression and evaluation of therapeutic agents for gum disease. This model demonstrates physiological tissue structure and response to inflammatory triggers, providing a platform for identification of new therapeutic targets.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Federica Romano, Giovanni Iaderosa, Matteo Corana, Stefano Perotto, Giacomo Baima, Federica Di Scipio, Giuliana Abbadessa, Giulia Maria Mariani, Mario Aimetti, Giovanni Nicolao Berta
Summary: This study analyzed the elemental composition of gingival crevicular fluid (GCF) and saliva and found differences in their content that could be used to diagnose periodontitis and periodontal health. GCF showed higher clustering potential than saliva.
Article
Chemistry, Multidisciplinary
Alina-Ramona Dimofte, Dorin Nicolae Gheorghe, Dora Maria Popescu, Ioana Mitrut, Petre Costin Marasescu, Horia Octavian Manolea, Mihail Virgil Boldeanu, Petra Surlin
Summary: This study evaluated the influence of metal-ceramic crowns on periodontal parameters and inflammatory marker levels. The results showed that metal-ceramic crowns can cause changes in periodontal clinical parameters and are associated with inflammatory marker levels.
APPLIED SCIENCES-BASEL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Jie Zhang, Wenjing Li, Hongye Lu, Ruifang Lu, Yalin Zhan, Huanxin Meng
Summary: The level of platelets in the GCF of patients with periodontitis is higher than that of healthy individuals. Platelets can interact with periodontal pathogens and neutrophils, and promote the formation of NETs.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
