Article
Engineering, Environmental
Kaiyue Huo, Yujie Liu, Rui Huang, Yiting Zhang, Honglu Liu, You Che, Chao Yang
Summary: A novel promoter engineering-based strategy was developed to create efficient PNP-mineralizing bacteria by introducing a complete PNP biodegradation pathway from Pseudomonas sp. strain WBC-3 into the genome of a biosafety and soil-dwelling bacterium Pseudomonas putida KT2440. Five strong promoters were identified from P. putida KT2440 and independently inserted into the genome to create a mutant strain with the highest PNP degradation rate and strong tolerance against high concentrations of PNP, demonstrating complete and efficient PNP mineralization. This strategy opens new avenues for creating efficient degraders for bioremediation.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Yuxin Zhao, You Che, Fang Zhang, Jiacheng Wang, Weixia Gao, Tong Zhang, Chao Yang
Summary: In this study, an efficient pathway construction strategy for integrating synthetic biodegradation pathways into the chromosome of Pseudomonas putida KT2440 was developed. The strategy resulted in improved chromosomal integration efficiencies and highlighted the potential for in situ bioremediation. Additionally, the strategy can be expanded to a broad range of bacterial species for widespread applications in bioremediation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Ion Iliuta, Maria C. Iliuta
Summary: Attaching microbial cells to micro-particles surface can enhance the biodegradation efficiency in packed-bed column bioreactors, especially under low phenol concentrations and liquid velocities.
Article
Environmental Sciences
Julieta Celeste Martin Tempestti, Harshavardhan Mohan, Pavithra Muthukumar Sathya, Se-Won Lee, Janaki Venkatachalam, Byung-Taek Oh, Kamala-Kannan Seralathan
Summary: In this study, Enterococcus gallinarum (JT-02) was isolated and identified from animal farm waste sludge, showing the ability to biodegrade p-nitrophenol (PNP). The optimal conditions for PNP biodegradation by strain JT-02 were determined through single-factor experiments. It was found that the best setups included PNP concentration of 100 mg/L, pH 7, temperature of 30 degrees C, agitation speed of 150 rpm, and inoculum dose of 3% (v/v). Under these conditions, the bacteria degraded 98.21% of PNP within 4 days. The intermediates produced during PNP biodegradation were identified using High Performance Liquid Chromatography (HPLC) analysis, and the biodegradation pathway was elucidated. Phytotoxicity studies with Vigna radiata seeds confirmed the applicability and efficiency of PNP biodegradation.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Saba Miri, Seyyed Mohammadreza Davoodi, Mohammad Hossein Karimi Darvanjooghi, Satinder Kaur Brar, Tarek Rouissi, Richard Martel
Summary: The study investigated the co-metabolic biodegradation of p-xylene by Pseudomonas putida in the presence of Benzene, Toluene, and Ethylbenzene. The GMDH model was proposed to predict the transformation of non-growth substrates in the presence of multiple growth substrates, showing potential for accurately predicting the degradation process in co-metabolic systems.
PROCESS BIOCHEMISTRY
(2021)
Article
Environmental Sciences
Kaiyue Huo, Siqi Wang, Wanwan Zhao, Hongfu Guo, Weini Xiong, Ruihua Liu, Chao Yang
Summary: In this study, the first engineered DCA-mineralizing strain KTU-P8DCA was constructed by enhancing pathway expression with a strong promoter P8 in the biosafety strain Pseudomonas putida KT2440. Strain KTU-P8DCA exhibited superior tolerance to high concentrations of DCA and showed excellent genetic stability in continuous passage culture. This research has significant implications for the bioremediation of sites heavily contaminated with DCA.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Ponruedee Kumkaew, Pitchaya Suaisom, Kanokwan Mukkata, Sirichai Koonaphapdeelert, Chayanon Sawatdeenarunat, Saoharit Nitayavardhana
Summary: This study uses Pseudomonas putida strains isolated from activated sludge to reduce the organic compound content and dark color of biogas plant effluent. The treatment of effluent from distillery wastewater anaerobic digestion with P. putida showed significant decolorization and organic removal efficiencies of 34.29% and 38.94%, respectively. The naturally selected bacterium can tolerate high levels of organic content and work synergistically with other microorganisms in the biogas plant effluent.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Mourad Fedeila, Zohra Hachaichi-Sadouk, Luis Fernando Bautista, Raquel Simarro
Summary: Two bacterial strains, Pseudomonas aeruginosa and Pseudomonas putida, isolated from industrial effluents in El-Harrach, Algeria, were able to degrade clopidogrel bisulfate under aerobic conditions. The consortium showed high efficiency in removing clopi-dogrel bisulfate at concentrations ranging from 5 to 1500 mg/L within 96 hours. The study also demonstrated the influence of pH, temperature, and supplemental sources of carbon and nitrogen on the biodegradation of clopidogrel bisulfate.
JOURNAL OF CONTAMINANT HYDROLOGY
(2023)
Article
Agricultural Engineering
Shoushuai Feng, Liangqi Gong, Yanke Zhang, Yanjun Tong, Hailing Zhang, Deqiang Zhu, Xing Huang, Hailin Yang
Summary: Bioaugmentation using a bacterial consortium of Pseudomonas putida SW-3 and Rhodococcus ruber SS-4 successfully degraded benzene, toluene, and styrene pollutants with an efficiency of up to 97.9%. The balanced bacterial community structure established after intervention led to an increase in the abundance of BTS-degrading bacteria, enhancing the overall degradation capability of the bacterial community.
BIORESOURCE TECHNOLOGY
(2021)
Article
Physics, Fluids & Plasmas
Ion Santra, Urna Basu, Sanjib Sabhapandit
Summary: This study investigates four distinct dynamical regimes of active Brownian motion in two dimensions, characterized by the presence of two timescales set by the rotational diffusion constant and the reversal rate. Analytical computation of position distribution and persistence exponents reveals that in regime II, the position distribution orthogonal to the initial orientation can be described using a scaled variable, showing nontrivial scaling behavior with a special scaling function. The exact first-passage time distribution computation demonstrates the emergence of a persistence exponent of 1 due to direction reversal in this regime.
Article
Engineering, Environmental
Jiayi Tong, Li Cui, Danqi Wang, Xin Wang, Zhaokun Liu
Summary: In this study, the membrane aerated biofilm reactor (MABR) was used to degrade high concentrations of PNP without external carbon sources. The MABR showed a strong shockproof ability and achieved high removal efficiencies of PNP and COD. The high-throughput sequencing results revealed the presence of CPR bacteria, which played a key role in the degradation of high concentrations of PNP.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Saurabh Gangola, Samiksha Joshi, Saurabh Kumar, Barkha Sharma, Anita Sharma
Summary: The study identified a bacterial isolate 2D with high tolerance and effective biodegradation rate for multiple pesticides. Analysis showed the highest similarity of 2D with Bacillus cereus, and the study on laccase under pesticide stress conditions suggested significantly increased enzyme production.
Article
Environmental Sciences
Leonardo Stari, Tanmoy Roy Tusher, Chihiro Inoue, Mei-Fang Chien
Summary: This study reports the enrichment of a stable aerobic carbon tetrachloride-degrading bacterial consortium for the first time, and identifies a predominant Pseudomonas strain in this consortium. The isolated Pseudomonas strain has the potential for in-situ bioremediation of carbon tetrachloride-contaminated environments under aerobic conditions.
Article
Agronomy
Stefania Fontanazza, Alessia Restuccia, Giovanni Mauromicale, Aurelio Scavo, Cristina Abbate
Summary: The study aimed to identify microorganisms responsible for degrading BP mulch films through a combination of biodegradation and molecular tests. A field trial revealed a higher abundance of Pseudomonas putida in BP plots, showing potential for accelerating the degradation of BP mulch films and reducing plastic pollution in agriculture.
Article
Biology
Dharman Kalaimurugan, Palaniappan Sivasankar, Kaliannan Durairaj, Manokaran Lakshmanamoorthy, Sulaiman Ali Alharbi, Sulaiman A. Al Yousef, Arunachalam Chinnathambi, Srinivasan Venkatesan
Summary: This study investigated a low-cost method using Pseudomonas sp. and gum arabic for degrading organic 4-nitrophenol pollutants, identifying a promising Pseudomonas sp. YPS 3 through experiments, and conducting in-depth research on the biodegradation mechanism.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Chemistry, Organic
Manne Madhava Reddy, Akella Sivaramakrishna
JOURNAL OF HETEROCYCLIC CHEMISTRY
(2020)
Article
Chemistry, Inorganic & Nuclear
Pothini Suman, Mohsin Y. Lone, Sannapaneni Janardan, Prakash C. Jha, Akella Sivaramakrishna, Hadley S. Clayton
JOURNAL OF COORDINATION CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Manne Madhava Reddy, Akella Sivaramakrishna
Article
Chemistry, Applied
Pothini Suman, Haamid R. Bhat, Sannapaneni Janardan, Sidhartha Protim Bortamuli, Prakash C. Jha, Akella Sivaramakrishna
APPLIED ORGANOMETALLIC CHEMISTRY
(2020)
Article
Chemistry, Physical
Sannapaneni Janardan, A. S. Vijai Anand, Pothini Suman, Mohsin Y. Lone, Prakash C. Jha, C. V. S. Brahmmananda Rao, Akella Sivaramakrishna
Summary: This study demonstrates the metal-free reduction of pentavalent tetracoordinate phosphine oxides to trivalent phosphines using inexpensive hydrosilanes in the presence of various activators. The reactivity of silanes as reducing agents is influenced by the oxophilic/electrophilic nature of the silicon atom. The presence of a hydrazide precursor with CH(3)SiHCl(2) has proven to be more effective in the reduction of phosphine oxides.
Article
Biotechnology & Applied Microbiology
Sujithra Vijayakumar, Rajani Raj, Uma Shaanker, Akella Sivaramakrishna, Siva Ramamoorthy
Summary: A novel lactone pigment isolated from an endophytic fungus in Bixa orellana L. leaves shows great potential for industrial applications due to its optimal growth conditions and high safety demonstrated in acute oral toxicity tests, making it a sustainable source for natural colorant in industries.
Article
Chemistry, Multidisciplinary
E. Veerashekhar Goud, A. S. Vijai Anand, Raghavaiah Pallepogu, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Akella Sivaramakrishna
Summary: A series of bisphosphoramides with different metal complexes were synthesized and characterized by spectroscopic and analytical data. The coordination modes of La(III) and Th(IV) complexes differed, and the extraction behavior of these ligands with Am(III), U(VI), Th(IV), and Pu(IV) was evaluated.
Review
Chemistry, Multidisciplinary
T. Boominathan, Akella Sivaramakrishna
Summary: Chitosan is a well-known biocompatible and biodegradable polysaccharide with a wide range of applications. It can be used to make various biomaterials and has shown great potential in wastewater treatment, drug delivery, and organic catalytic reactions.
TOPICS IN CURRENT CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Joydip Mondal, Akella Sivaramakrishna
Summary: Molecules with triazine and tetrazine moieties are important in biological applications due to their multifunctional and therapeutic potential. Various synthetic routes can produce diverse derivatives with different electronic properties. These compounds have broad prospects for applications in the field of biomedicine.
TOPICS IN CURRENT CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Manne Madhava Reddy, Rajagopal Desikan, Sanjay Naik, Sanjit Kumar, D. Thirumal Kumar, C. George Priya Doss, Akella Sivaramakrishna
Summary: The inhibition of PIK3CA protein by quinazolinone derivatives shows promising anti-cancer activity and could be used in the treatment of breast cancer. The in vitro study demonstrated that these derivatives have stronger anti-cancer activity compared to the currently available drug. This research provides new insights for cancer treatment.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Chemistry, Multidisciplinary
B. B. Pavankumar, Prabodh Ranjan, Prakash C. Jha, Akella Sivaramakrishna
Summary: A series of new oxoquinoline-imidazole derivatives were synthesized and employed as fluorescence signaling switches, allowing for rapid detection of Zn2+ and Fe3+ ions through an ESIPT mechanism. The compounds were successfully applied for the detection of Zn2+ and Fe3+ ions in cells, showing potential biomedical applications.
Article
Chemistry, Multidisciplinary
Madhava Reddy Manne, Rakesh R. Panicker, Kumar Ramakrishnan, Hima M. K. Hareendran, Sudhir Kumar Pal, Sanjit Kumar, Raghavaiah Pallepogu, Rajagopal Desikan, Akella Sivaramakrishna
Summary: In this study, a new strategy for the synthesis of quinoline-based quinazolinones catalytically using InCl3 was developed. The structures and purities of the synthesized compounds were assessed, and the compounds were found to possess anti-inflammatory and anti-cancer activities. Furthermore, computational docking studies were performed to evaluate the binding abilities of the synthesized compounds with the Sirt1 protein.
Article
Chemistry, Inorganic & Nuclear
Selva R. Kumar, Vetriarasu Venkatesan, R. Bhaskar, S. K. Ashok Kumar, Akella Sivaramakrishna, Kari Vijayakrishna, C. V. S. Brahmmananda Rao, N. Sivaraman, Suban K. Sahoo
Summary: A simple and reliable colorimetric probe has been synthesized and its sensing ability towards various metal ions has been studied through colorimetry. The probe shows significant color changes upon interaction with uranyl ions, enabling selective and sensitive visual detection of trace levels of UO22+ ions without the need for sophisticated instruments.
DALTON TRANSACTIONS
(2021)
Article
Chemistry, Analytical
B. B. Pavankumar, Prabodh Ranjan, Prakash C. Jha, Akella Sivaramakrishna
Article
Chemistry, Multidisciplinary
Premkumar Jayaraj, Gopal Venkatesh Shavi, Ananda Kumar Srinivasan, Ramesh Raghavendra, Akella Sivaramakrishna, Rajagopal Desikan
NEW JOURNAL OF CHEMISTRY
(2020)
