Article
Reproductive Biology
Anna Z. Szostek-Mioduchowska, Haruki Shiotani, Yuki Yamamoto, Agnieszka Sadowska, Anna Wojtowicz, Keisuke Kozai, Takuo Hojo, Koji Kimura, Dariusz J. Skarzynski, Kiyoshi Okuda
Summary: This study found that cortisol suppresses the synthesis of PGF(2 alpha) in the equine endometrium by affecting the arachidonic acid cascade.
Article
Reproductive Biology
Heather L. Chaney, Lindsay F. Grose, Gilles Charpigny, Susanta K. Behura, I. Martin Sheldon, James G. Cronin, Patrick Lonergan, Thomas E. Spencer, Daniel J. Mathew
Summary: This study identified biologically relevant IFNT-stimulated genes in specific endometrial cell types. The findings provide critical information on the effects of conceptus IFNT on specific endometrial compartments during early developmental processes in cattle.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Piotr Kaczynski, Vera van der Weijden, Ewelina Goryszewska-Szczurek, Monika Baryla, Susanne E. Ulbrich, Agnieszka Waclawik
Summary: This study found that CpG methylation induced by embryonic signals may contribute to regulating endometrial gene expression during early pregnancy in pigs.
BIOLOGY OF REPRODUCTION
(2022)
Review
Agriculture, Dairy & Animal Science
H. Tinning, J. C. Edge, T. H. C. DeBem, F. Deligianni, G. Giovanardi, V. Pensabene, F. V. Meirelles, N. Forde
Summary: The endometrium plays a crucial role in successful pregnancy in cattle, contributing to various aspects such as regulating the estrus cycle, nourishing the conceptus, creating a receptive microenvironment, providing support, and affecting the mother systemically. Further research and exploration of in vitro models are needed to enhance our understanding of conceptus-maternal interactions and improve food production efficiency while reducing environmental burden.
Article
Reproductive Biology
Heather L. Chaney, Lindsay F. Grose, Jeanna M. LaBarbara, Adam W. Sirk, Alyssa M. Blancke, Jose M. Sanchez, Claudia Passaro, Patrick Lonergan, Daniel J. Mathew
Summary: Bovine and ovine conceptuses express galectin-1, which induces endometrial gene expression related to maternal immune tolerance in cattle. Expression of galectins in the endometrium varies during pregnancy in cows and sheep, suggesting important roles in maternal-conceptus immune tolerance in ruminants.
BIOLOGY OF REPRODUCTION
(2022)
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Eleanore O'Neil, Thomas E. Spencer
Summary: This study found that certain lipid components are reduced in the endometrium of pregnant ewes, while certain lipid compounds are elevated in the uterine lumen. Additionally, elevated levels of amino acids, carbohydrates, and energy substrates were observed in the uterine lumen of pregnant ewes.
BIOLOGY OF REPRODUCTION
(2021)
Article
Multidisciplinary Sciences
Alba Rudolf Vegas, Meriem Hamdi, Giorgia Podico, Heinrich Bollwein, Thomas Froehlich, Igor F. Canisso, Stefan Bauersachs, Carmen Alminana
Summary: This study identified specific molecules packed in uterine extracellular vesicles (uEVs) associated with maternal recognition of pregnancy (MRP) in mares, providing additional knowledge in this area. Further analysis is needed to confirm the role of these molecules in MRP in mares.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Yuri Park, Sang Jun Han
Summary: Endometriosis is an estrogen-dependent inflammatory disease that causes pelvic pain and infertility in women. Immune system defects may contribute to the progression of endometriosis. Elevated levels of interferon, a cytokine, play a crucial role in endometriosis progression, but also have a critical role in normal endometrial function, particularly in pregnancy initiation and maintenance.
Article
Veterinary Sciences
Wonchul Jung, Inkyu Yoo, Jisoo Han, Minjeong Kim, Soohyung Lee, Yugeong Cheon, Minsun Hong, Bo-Young Jeon, Hakhyun Ka
Summary: Research has shown that caspases are dynamically expressed in the endometrium throughout the estrous cycle and at the maternal-conceptus interface during pregnancy in pigs. The expression of CASP3, CASP6, and CASP7 changes in a stage-specific manner in the endometrium during different reproductive stages. Additionally, the expression of caspases in endometrial cells is influenced by steroid hormones and cytokines, suggesting that caspase action may play an important role in regulating endometrial and placental function in pigs during the implantation period.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Developmental Biology
Pascal Philibert, Stephanie Dejardin, Nelly Pirot, Alain Pruvost, Anvi Laetitia Nguyen, Florence Bernex, Francis Poulat, Brigitte Boizet-Bonhoure
Summary: This study shows that the PGD2 pathway plays a major role in protecting the endometrium against adenomyosis development in the uterus. Lack of PGD2 leads to dysregulation of PGE2 production and the steroidogenic pathway. Additional experiments are needed to fully understand the molecular mechanisms and confirm if the mouse strain is a suitable model for studying human adenomyosis.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Endocrinology & Metabolism
Rulan Bai, Kazuya Kusama, Yuta Matsuno, Hanako Bai, Toshihiro Sakurai, Koji Kimura, Kazuhiko Imakawa
Summary: In this study, it was found that prostaglandin E2 (PGE2) activates the cyclic AMP (cAMP) signaling pathway in bovine endometrial stromal cells, leading to the upregulation of early pregnancy-related genes. The activation of cAMP resulted in the upregulation of transcripts related to transcription regulation, nucleus, and mitotic spindle organization. The transcription factors NFIL3, CEBPA, and HIF1A were found to be regulated by cAMP and involved in the expression of PTGS1/2 and IGFBP1/3. Furthermore, NFIL3 and CEBPA were localized in the endometrial stroma on day 17 of pregnancy, but not on day 17 of the estrous cycle.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Sroisuda Chotimanukul, Junpen Suwimonteerabutr, Mongkol Techakumphu, Theerawat Swangchan-Uthai
Summary: This study investigated the effects of elevated temperature on bovine endometrial cell cultures. The findings suggest that heat exposure can compromise the immune response and prostaglandin synthesis of the endometrium, leading to reduced fertility.
Article
Veterinary Sciences
Charlotte Gibson, M. de Ruijter-Villani, Tom A. E. Stout
Summary: In the equine pre-implantation development, the insulin-like growth factors (IGFs) system plays an important role in preparing the endometrium for implantation and regulating conceptus growth and development. The presence and down-regulation of IGFs, their receptors, and IGF binding proteins (IGFBPs) in both the endometrium and conceptus suggest their involvement in conceptus-maternal communication during the preparation for implantation.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Reproductive Biology
Hwanhee Jang, Soohyung Lee, Inkyu Yoo, Yohan Choi, Jisoo Han, Yugyeong Cheon, Hakhyun Ka
Summary: S100A8, S100A9, and S100A12, calcium-binding proteins, are dynamically expressed in response to conceptus-derived signals at the maternal-conceptus interface, potentially playing a critical role in regulating endometrial epithelial cell function and conceptus implantation to support the establishment and maintenance of pregnancy in pigs.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Susanta K. Behura, Andrew M. Kelleher, Thomas E. Spencer
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2020)
Article
Biochemistry & Molecular Biology
Emmanuel N. Paul, Gregory W. Burns, Tyler J. Carpenter, Joshua A. Grey, Asgerally T. Fazleabas, Jose M. Teixeira
Summary: Transcriptome analysis revealed significant phenotypic and gene expression differences between fibroid-associated myometrium and non-diseased myometrium, particularly in upregulated genes related to extracellular matrix and structure organization. These results suggest that fibroid studies should consider using both matched myometrium and non-diseased myometrium as controls to better understand the pathophysiology of uterine fibroids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Pramod Dhakal, Thomas E. Spencer
Summary: Prss28 and Prss29 are not essential for female fertility and do not affect endometrial gland development or uterine function in mice.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2021)
Article
Multidisciplinary Sciences
Constantine A. Simintiras, Pramod Dhakal, Chaman Ranjit, Harriet C. Fitzgerald, Ahmed Z. Balboula, Thomas E. Spencer
Summary: The study developed a high-throughput method for isolating intraorganoid fluid (IOF) from human endometrial epithelial organoids, revealing distinct biochemical differences between IOF and extraorganoid fluid (EOF). This has the potential to enhance our understanding of uterine fluid composition and regulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Pramod Dhakal, Harriet C. Fitzgerald, Andrew M. Kelleher, Hongyu Liu, Thomas E. Spencer
Summary: The study demonstrated the crucial roles of uterine glands in embryo survival and stromal cell decidualization. Using a glandless FOXA2-deficient mouse model, researchers found compromised embryo survival and decidualization on gestational day 6.5, leading to abrupt pregnancy loss by day 7.5.
Article
Biochemistry & Molecular Biology
Chelsie B. Steinhauser, Colleen A. Lambo, Katharine Askelson, Gregory W. Burns, Susanta K. Behura, Thomas E. Spencer, Fuller W. Bazer, Michael Carey Satterfield
Summary: Placental development is modified in response to maternal nutrient restriction, leading to different fetal growth rates. Differences in gene expression and function were found between SGA and NR NonSGA placentomes, with overrepresentation of genes related to natural-killer-cell-mediated cytotoxicity in SGA placentomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Constantine A. Simintiras, Jessica N. Drum, Hongyu Liu, M. Sofia Ortega, Thomas E. Spencer
Summary: Research suggests that the uterine lumen fluid (ULF) has a degree of metabolic autonomy, which enhances our understanding of the mechanisms behind pregnancy establishment. ULF plays a crucial role in successful pregnancy establishment and maintenance, impacting the wellbeing of offspring into adulthood. These findings have significant implications for improving fertility and pregnancy outcomes in both domestic animals and women.
COMMUNICATIONS BIOLOGY
(2022)
Article
Developmental Biology
Andrew M. Kelleher, Carolyn C. Allen, Daniel J. Davis, Thomas E. Spencer
Summary: This study describes a new mouse model that is useful for studying uterine gland function during pregnancy. The researchers inserted Cre recombinase into a specific gene using CRISPR-Cas9 genome editing, allowing for targeted expression of Cre recombinase. Additionally, conditional deletion of a key gene in the uterine glands using this mouse model confirmed the value of Prss29-Cre mice in elucidating and exploring adult uterine gland function.
Review
Biochemistry & Molecular Biology
Rodney D. Geisert, Destiny N. Johns, Caroline A. Pfeiffer, Riley M. Sullivan, Caroline G. Lucas, Constantine A. Simintiras, Bethany K. Redel, Kevin D. Wells, Thomas E. Spencer, Randall S. Prather
Summary: CRISPR-Cas9 gene editing technology allows for the study of specific genes involved in reproductive regulation, particularly in the establishment and maintenance of pregnancy. Research on gene editing of porcine conceptuses provides new insights into the regulation of endometrial function, while in vitro studies using endometrial organoids offer an alternative method for studying specific endometrial genes.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2023)
Article
Medicine, Research & Experimental
Emmanuel N. Paul, Joshua A. Grey, Tyler J. Carpenter, Zachary B. Madaj, Kin H. Lau, Scott A. Givan, Gregory W. Burns, Ronald L. Chandler, Ganesa R. Wegienka, Hui Shen, Jose M. Teixeira
Summary: Uterine fibroids disproportionately affect Black women compared to women of other races and ethnicities. This study examined the molecular profiles of myometrium and fibroids from Black and White women, and found differences in gene expression and DNA methylation patterns between the two races. The results suggest that the molecular basis for the racial disparity in uterine fibroids may lie in the myometrium rather than the fibroids themselves.
Article
Obstetrics & Gynecology
Kentaro Kai, Niraj R. Joshi, Gregory W. Burns, Samantha M. Hrbek, Erin L. Vegter, Maria Ariadna Ochoa-Bernal, Yong Song, Genna E. Moldovan, Lorenzo F. Sempere, Eduardo H. Miyadahira, Paulo C. Serafini, Asgerally T. Fazleabas
Summary: This study explores the role of miR-210 and its targets, IGFBP3 and COL8A1, in the growth and development of endometriosis. It was found that miR-210 expression was decreased in ectopic lesions, while IGFBP3 and COL8A1 expression was increased. MiR-210 overexpression suppressed IGFBP3 expression and attenuated cell proliferation and migration.
REPRODUCTIVE SCIENCES
(2023)
Article
Medicine, Research & Experimental
Yong Song, Gregory W. Burns, Niraj R. Joshi, Ripla Arora, J. Julie Kim, Asgerally T. Fazleabas
Summary: The development and progression of endometriotic lesions are poorly understood, but immune cell dysfunction and inflammation are closely associated with the pathophysiology of endometriosis. In this study, a 3D in vitro model using endometriotic spheroids (ES) was developed to explore the role of epithelial-stromal interactions and model peritoneal invasion associated with lesion development. Transcriptomic analysis revealed inflammation-related pathways and significant overlap with baboon endometriotic lesions. Furthermore, a model with human peritoneal mesothelial cells in an extracellular matrix was developed to mimic invasion of endometrial tissue into the peritoneum.
Review
Agriculture, Dairy & Animal Science
K. M. Davenport, M. S. Ortega, G. A. Johnson, H. Seo, T. E. Spencer
Summary: Ruminants have a unique placental structure that involves the attachment of binucleate cells to the uterine epithelium to form placental cotyledons. This understanding is crucial in analyzing pregnancy loss and improving pregnancy outcomes in ruminant animals.
Article
Reproductive Biology
Kimberly M. Davenport, Eleanore ONeil, M. Sofia Ortega, Amanda Patterson, Andrew M. Kelleher, Wesley C. Warren, Thomas E. Spencer
Summary: This study utilized single-cell transcriptome analysis to identify different cell types and their ontogeny in bovine conceptus and chorion. The research revealed the role of key transcription factors in the development of trophoblasts and differentiation of binucleate cells. The digital atlas of cell types in the developing bovine conceptus serves as a valuable resource for understanding the genes and pathways involved in its development during critical periods.
BIOLOGY OF REPRODUCTION
(2023)
Article
Developmental Biology
Harriet C. Fitzgerald, Andrew M. Kelleher, Chaman Ranjit, Danny J. Schust, Thomas E. Spencer
Summary: Uterine glands and their secretions play a crucial role in various reproductive processes, such as uterine receptivity, blastocyst implantation, and placental development. Hormones like estrogen and progesterone, as well as external factors, regulate the function of uterine glands. A study using a human endometrial epithelial organoid system found that different proteins secreted by the organoids were modulated by hormone and prostaglandin E2. Additionally, one of the secreted proteins, cystatin C, was shown to attenuate the stromal decidualization response. These findings highlight the impact of uterine gland-derived factors on stromal cell decidualization and its implications for pregnancy establishment and fertility in women.
MOLECULAR HUMAN REPRODUCTION
(2023)