Review
Oncology
Yunke Wang, Fuming Qiu, Jun Yan
Summary: In innate immune cells, the transcription factor c-Maf plays a crucial role in regulating cell fate and function, influencing immune homeostasis and antitumor immunity. This article discusses recent breakthroughs in c-Maf research, particularly its impact on the immunosuppressive tumor microenvironment (TME) and proposes c-Maf-targeted cancer immunotherapy as a potential strategy.
Article
Multidisciplinary Sciences
Xiangjia Zhu, Yu Du, Dan Li, Jie Xu, Qingfeng Wu, Wenwen He, Keke Zhang, Jie Zhu, Linying Guo, Ming Qi, Ailin Liu, Jiao Qi, Guangyu Wang, Jiaqi Meng, Zhenglin Yang, Kang Zhang, Yi Lu
Summary: The study demonstrates that high myopia is associated with an increased equatorial diameter of the lens, which is linked to an up-regulation of crystallin expression driven by the transcription factor MAF and TGF-beta 1 signaling, providing insights for therapeutic interventions.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Lauren B. Rodda, Peter A. Morawski, Kurt B. Pruner, Mitchell L. Fahning, Christian A. Howard, Nicholas Franko, Jennifer Logue, Julie Eggenberger, Caleb Stokes, Inah Golez, Malika Hale, Michael Gale, Helen Y. Chu, Daniel J. Campbell, Marion Pepper
Summary: Research found that individuals previously infected with SARS-CoV-2 generate more specific memory B cells, variant-neutralizing antibodies, and a distinct population of memory CD4(+) T cells compared to those who were previously naive. Additional vaccination does not replicate the unique CD4(+) T cell cytokine profile observed in previously infected individuals.
Review
Genetics & Heredity
Mitsunori Fujino, Masami Ojima, Satoru Takahashi
Summary: This review provides a detailed exploration of the functions of MAF transcription factors studied in vivo, as well as the pathology of human patients and mouse models with point mutations in c-MAF, MAFA, and MAFB. The generation of genetically modified mice lacking these factors has allowed for analysis of their phenotypes and has highlighted their potential as models for studying the pathophysiology and development of targeted therapies for diseases caused by MAF mutations.
Article
Biochemistry & Molecular Biology
Zhuo Li, Yingying Tian, Lei Zhang, Tianqi Zhang, Peng Wang, Jingfeng Wang
Summary: The study demonstrates that SCII can accelerate cartilage repair and new bone formation by activating muscle paracrine factors, thus positively impacting fracture healing.
Article
Cell Biology
Debashree Chatterjee, Yuwei Zhang, Christ-Dominique Ngassaki-Yoka, Antoine Dutilleul, Soumia Khalfi, Olivier Hernalsteens, Tomas Raul Wiche Salinas, Jonathan Dias, Huicheng Chen, Yasmine Smail, Jean-Philippe Goulet, Brendan Bell, Jean-Pierre Routy, Carine Van Lint, Petronela Ancuta
Summary: The aryl hydrocarbon receptor (AhR) inhibits HIV-1 replication and outgrowth in TCR-activated CD4+ T cells. AhR blockade improves early/late reverse transcription, integration/translation, and viral outgrowth in CD4+ T cells of people living with HIV-1 receiving antiretroviral therapy. AhR blockade downregulates genes/pathways involved in HIV-1 interaction and gut-homing in CD4+ T cells of ART-treated PLWH, and HIC1 is identified as a direct AhR target.
Article
Cell Biology
Nathalie G. M. Thielen, Margot Neefjes, Elly L. Vitters, Henk M. van Beuningen, Arjen B. Blom, Marije I. Koenders, Peter L. E. M. van Lent, Fons A. J. van de Loo, Esmeralda N. Blaney Davidson, Arjan P. M. van Caam, Peter M. van der Kraan
Summary: During osteoarthritis, TGF-beta signaling pathways contribute to the hypertrophy-like phenotype in chondrocytes. This study found that ALK5 kinase activity is essential for the induction of crucial hypertrophy factors in TGF-beta-stimulated chondrocytes.
Article
Multidisciplinary Sciences
Shilu Zhang, Saptarshi Pyne, Stefan Pietrzak, Spencer Halberg, Sunnie Grace McCalla, Alireza Fotuhi Siahpirani, Rupa Sridharan, Sushmita Roy
Summary: The authors developed a method called single-cell Multi-Task Network Inference (scMTNI) to accurately infer the dynamics of cell type-specific Gene Regulatory Networks (GRNs) from scRNA-seq and scATAC-seq datasets. This method can identify key regulators and accurately model network dynamics for diverse processes such as cellular reprogramming and differentiation.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Magalie Michee-Cospolite, Marina Boudigou, Alexis Grasseau, Quentin Simon, Olivier Mignen, Jacques-Olivier Pers, Divi Cornec, Laetitia Le Pottier, Sophie Hillion
Summary: Regulatory B cells (Bregs) play important roles in various pathological conditions by producing IL-10 and utilizing multiple suppressing mechanisms to modulate immune response. The molecular regulation of Bregs is not fully understood, and this manuscript provides detailed insights into the role of transcription factors STAT3 and c-MAF in controlling IL-10 production in B cells and their significance in the Breg response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Joanna Janiszewska, Magdalena Bodnar, Julia Paczkowska, Adam Ustaszewski, Maciej J. Smialek, Lukasz Szylberg, Andrzej Marszalek, Katarzyna Kiwerska, Reidar Grenman, Krzysztof Szyfter, Malgorzata Wierzbicka, Maciej Giefing, Malgorzata Jarmuz-Szymczak
Summary: The study demonstrates that miR-1290 suppresses MAF expression in LSCC, revealing the underlying interaction between the two. Immunohistochemistry staining showed loss of nuclear MAF expression in 58% of LSCC samples. Through data analysis, it was determined that loss-of-function mutations, promoter region DNA methylation or CNV are not responsible for MAF loss in LSCC.
Article
Biochemistry & Molecular Biology
Manuela Minguzzi, Veronica Panichi, Stefania D'Adamo, Silvia Cetrullo, Luca Cattini, Flavio Flamigni, Erminia Mariani, Rosa Maria Borzi
Summary: Notch signaling pathway plays a critical role in cartilage development and homeostasis, with NOTCH1 being the key receptor that can affect the health status of osteoarthritis chondrocytes by regulating proliferation, hypertrophy, and terminal differentiation. Silencing NOTCH1 showed a healthier phenotype in OA chondrocytes, suggesting it is a convenient therapeutic target to attenuate OA progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Abazar Esmaeili, Samaneh Hosseini, Amir Kamali, Maryam Hosseinzadeh, Faezeh Shekari, Mohamadreza Baghaban Eslaminejad
Summary: This study evaluated the therapeutic effect of extracellular vesicles (EVs) harvested from aggregates of rabbit mesenchymal stem cells (MSCs), chondrocytes, and their co-aggregates in a rat osteoarthritis model. The results demonstrated that EVs harvested from the co-aggregation of MSCs and chondrocytes could be considered as a new therapeutic potential for treating osteoarthritis.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Tong Zhang, Kaoru Yamagata, Shigeru Iwata, Koshiro Sonomoto, Gulzhan Trimova, Anh Phuong Nguyen, He Hao, Yu Shan, Mai-Phuong Nguyen, Shingo Nakayamada, Yoshiya Tanaka
Summary: Mesenchymal stem cells (MSC) have the ability to differentiate into chondrocytes, and EBI3, a protein expressed during chondrogenic differentiation, plays a role in this process by regulating ER stress sensor IRE1 alpha.
Article
Hematology
Vanya Icheva, Johanna Ebert, Ulrich Budde, Gesa Wiegand, Sarah Schober, Juliane Engel, Matthias Kumpf, Karl Jaschonek, Felix Neunhoeffer, Jorg Michel, Christian Schlensak, Michael Hofbeck, Harry Magunia
Summary: This study investigated the diagnostic tools and impact of acquired von Willebrand syndrome (aVWS) on intraoperative bleeding during pediatric cardiac surgery. The glycoprotein Ib M assay (GPIbM)/von Willebrand factor antigen (VWF:Ag) ratio was found to be the most reliable predictor of aVWS. Patients with intraoperative aVWS required more fresh frozen plasma and fibrinogen concentrate for treatment. These findings provide a basis for further research on the effects and potential treatment of aVWS.
Article
Hematology
Yujia Xu, Min Xu, Jiefei Tong, Xiaowen Tang, Jinhao Chen, Xuehan Chen, Zubin Zhang, Biyin Cao, A. Keith Stewart, Michael F. Moran, Depei Wu, Xinliang Mao
Summary: The study reveals that the Otub1/c-Maf axis could be a potential therapeutic target for multiple myeloma, with Otub1 preventing c-Maf degradation and enhancing its transcriptional activity, and LanC inhibiting c-Maf deubiquitination and inducing its degradation to suppress MM growth and prolong overall survival.
Article
Surgery
Ramin Fallahzadeh, Franck Verdonk, Ed Ganio, Anthony Culos, Natalie Stanley, Ivana Maric, Alan L. Chang, Martin Becker, Thanaphong Phongpreecha, Maria Xenochristou, Davide De Francesco, Camilo Espinosa, Xiaoxiao Gao, Amy Tsai, Pervez Sultan, Martha Tingle, Derek F. Amanatullah, James Huddleston, Stuart B. Goodman, Brice Gaudilliere, Martin S. Angst, Nima Aghaeepour
Summary: By combining high-fidelity accelerometry and advanced immune profiling, it is possible to accurately track individualized physical recovery trajectories in patients after surgery and link them to preoperative immune status.
Article
Orthopedics
Rosa S. Valtanen, Katherine L. Hwang, Derek F. Amanatullah, James I. Huddleston, William J. Maloney, Stuart B. Goodman
Summary: This study retrospectively analyzed long-term follow-up data of patients who underwent revision total hip arthroplasty (THA) using a cementless modular stem. The results demonstrated long-term clinical success and safety of the cementless modular revision stem for complex revision operations.
JOURNAL OF ARTHROPLASTY
(2023)
Article
Orthopedics
Maveric K. I. L. Abella, Chukwunweike N. Ezeanyika, Andrea K. Finlay, Derek F. Amanatullah
Summary: The COVID-19 pandemic has resulted in an increase in same-day discharge (SDD) for total joint arthroplasty, but it may not be beneficial for all patients. Further investigation into the safety of SDD and risk stratification is necessary for improved patient outcomes.
JOURNAL OF ARTHROPLASTY
(2023)
Article
Chemistry, Physical
Evgeny Ogorodnik, Arpad Karsai, Ying X. Liu, Jacopo Di Lucente, Yuqi Huang, Terell Keel, Dominik R. Haudenschild, Lee-Way Jin, Gang-Yu Liu
Summary: Cell motility can be triggered and regulated individually using transient mechanical stimulus applied to designated cells, as demonstrated by this study using BV2 microglial cells. The applied force's location and magnitude influence the cell's movement. Insights into the underlying mechanism of force-induced single cellular movement are revealed, involving myosin activation, actin cortex repair, focal adhesion degradation, lamellipodium detachment, and cell polarization and movement.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Orthopedics
Harsh N. Shah, Andrew A. Barrett, Andrea K. Finlay, Prerna Arora, Michael J. Bellino, Julius A. Bishop, Michael J. Gardner, Matthew D. Miller, James I. Huddleston, William J. Maloney, Stuart B. Goodman, Derek F. Amanatullah
Summary: The aim of this study was to compare the restoration of hip biomechanics between non-arthroplasty surgeons (NAS) and elective arthroplasty surgeons (EAS) through lateral offset, leg length, and acetabular component position. Retrospective review of 131 patients who underwent THA for femoral neck fracture by 7 EAS and 20 NAS was conducted. Results showed that NAS under-restored lateral femoral offset compared to the uninjured side, indicating a risk for under-restoration. However, there was no association found between surgeon type and other biomechanical factors. This study highlights the importance of considering lateral femoral offset in THA for femoral neck fractures, especially when performed by surgeons without regular experience in elective THA.
Article
Orthopedics
Rit Apinyankul, Alexander Y. Hui, Katherine Hwang, Nicole Alexandriadria Segovia, Derek F. Amanatullah, James I. Huddleston, William J. Maloney, Stuart B. Goodman
Summary: Conversion total knee arthroplasty (TKA) with retained periarticular hardware in patients with end-stage knee osteoarthritis can achieve excellent outcomes, but there are unique challenges. The evidence supporting retention versus removal of hardware during TKA is controversial.
JOURNAL OF ARTHROPLASTY
(2023)
Article
Orthopedics
Jonathan J. Lee, Kingsley Oladeji, Brian F. Sweeney, Tatenda L. Chakoma, Prerna Arora, Andrea K. Finlay, Derek F. Amanatullah
Summary: This study assessed the prevalence of recurrent, synchronous, and metachronous periprosthetic joint infection (PJI) in patients who had multiple arthroplasties and identified risk factors for a subsequent PJI. The results showed that over 20% of patients with multiple arthroplasties and a single PJI will develop a subsequent PJI, with 12% recurring in the initial arthroplasty and nearly 10% occurring in another arthroplasty. Tobacco use, bacteremia, and Staphylococcus aureus isolation at the time of the initial PJI were identified as particular risk factors.
JOURNAL OF ARTHROPLASTY
(2023)
Article
Orthopedics
Alpesh A. Patel, Joseph H. Schwab, Derek F. Amanatullah, Srikanth N. Divi
Summary: Artificial intelligence (AI) refers to machines exhibiting human-like intelligence, and it has already had a significant impact on industries such as entertainment, transportation, and communications. AI is also expected to influence medicine and healthcare. In the field of orthopaedics, interest in AI-based techniques has grown rapidly, and the technical nature of orthopaedic surgery makes it a potential area for AI to transform musculoskeletal care. However, orthopaedic surgeons lack knowledge about AI-based techniques and their application, which calls for a better understanding to identify potential pitfalls and improve trust in AI-based analytics.
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2023)
Review
Orthopedics
Ian Hollyer, David Ivanov, Shanthi Kappagoda, David W. Lowenberg, Stuart B. Goodman, Derek F. Amanatullah
Summary: Prosthetic joint infection (PJI) after total knee arthroplasty (TKA) is a common and challenging problem. Treatment for PJI depends on patient goals, characteristics, and infection timeline. Two-stage surgery with implant removal, debridement, and antibiotic spacer placement is the most common treatment. Static spacers are used for older, less healthy patients, while mobile spacers are used for younger, healthier patients. Customizable spacers with different antibiotics, cement, and designs are available. Understanding these options is important for optimizing treatment.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Orthopedics
Anne K. Haudenschild, Blaine A. Christiansen, Sophie Orr, Erin E. Ball, Christopher M. Weiss, Hongwei Liu, David P. Fyhrie, Jasper H. N. Yik, Lark L. Coffey, Dominik R. Haudenschild
Summary: The novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected over 650 million individuals globally, with approximately 23% developing long-lasting COVID symptoms. This study using a humanized mouse model of COVID-19 provides the first direct evidence of acute bone loss, increased osteoclast number, and thinner growth plates due to SARS-CoV-2 infection. Evaluating skeletal health in recovered COVID-19 patients will be crucial in identifying at-risk populations and developing effective countermeasures.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Surgery
Henry C. Cousins, Eli M. Cahan, Joshua T. Steere, William J. Maloney, Stuart B. Goodman, Matthew D. Miller, James I. Huddleston, Derek F. Amanatullah
Summary: Surgical team communication is crucial for operative efficiency, and factors such as team turnover and surgeon-staff preferences play a significant role. This retrospective analysis of total joint arthroplasties found that turnover among nonsurgical staff was associated with longer operative duration, while the presence of surgeon-preferred staff improved efficiency.
Article
Orthopedics
Ian Hollyer, Akaila C. C. Cabell, Stephen T. T. Duncan, Stefano M. P. Rossi, Peter K. K. Sculco, C. Lowry Barnes, Derek F. F. Amanatullah
Summary: The John N. Insall Knee Society Traveling Fellowship allows four international orthopedic surgeons with fellowship training in arthroplasty or sports to travel to knee surgery centers in North America for one month. The fellowship aims to promote research and education, as well as facilitate the exchange of ideas. The impact of such traveling fellowships on surgeon preferences has not been studied.
JOURNAL OF KNEE SURGERY
(2023)
Article
Clinical Neurology
Jennifer M. Hah, Julien D. Veron Vialard, Bradley Efron, Sean C. Mackey, Ian R. Carroll, Derek F. Amanatullah, Balasubramanian Narasimhan, Tina Hernandez-Boussard
Summary: This study aimed to analyze the impact of preoperative and postoperative risk factors on persistent postoperative pain and opioid use. The results showed a significant association between preoperative opioid use and delayed postoperative opioid cessation, while postoperative increases in depressive symptoms and pain catastrophizing were independently associated with delayed postoperative pain resolution. Therefore, risk stratification of patients before and after surgery can identify those at highest risk for these distinct outcomes, and interventions targeting these perioperative risk factors may prevent prolonged postoperative pain and opioid use.
Article
Surgery
Harsh N. N. Shah, Andrew A. A. Barrett, Patrick H. H. Le, Prerna Arora, Robin N. N. Kamal, Derek F. F. Amanatullah
Summary: This study aimed to determine the essential medical preferences for patients during their surgical care. The findings revealed that the metrics prioritized by hospitals and surgeons are not important to the majority of patients, highlighting the need for including patients in discussions with physicians and hospital systems.
PATIENT SAFETY IN SURGERY
(2023)
Article
Chemistry, Physical
Evgeny Ogorodnik, Arpad Karsai, Ying X. Liu, Jacopo Di Lucente, Yuqi Huang, Terell Keel, Dominik R. Haudenschild, Lee-Way Jin, Gang-Yu Liu
Summary: This work introduces a new means to trigger and regulate cell motility individually using transient mechanical stimulus applied to designated cells. Through investigation of BV2 microglial cells, it is found that motility can be reliably initiated using mechanical compression. The location and magnitude of the applied force impact the movement of the cell, providing new physical insights into the underlying mechanism of force-induced single cellular movement.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)