Article
Peripheral Vascular Disease
Gaizun Hu, Qing Zhu, Weili Wang, Dengpiao Xie, Chaoling Chen, Pin-Lan Li, Joseph K. Ritter, Ningjun Li
Summary: The study shows that the CD-S1PR1 pathway is crucial for sodium excretion and its deletion leads to sodium retention and exacerbates the development of salt-sensitive hypertension.
JOURNAL OF HYPERTENSION
(2021)
Article
Medicine, Research & Experimental
Syed J. Khundmiri, Carolyn M. Ecelbarger, Joycemary Amponsem, Hong Ji, Kathryn Sandberg, Dexter L. Lee
Summary: This research found that deletion of the PPAR-alpha gene leads to an increase in blood pressure in mice, with significantly higher NKA activity and protein expression in PPAR-alpha KO mice compared to WT mice. Angiotensin II further increased the expression and activity of NKA and aquaporin 1 in both genotypes. Angiotensin II decreased the expression of several important proteins in the renal cortex of both WT and PPAR-alpha KO mice, with a more significant reduction in NCC in PPAR-alpha KO mice.
Article
Cardiac & Cardiovascular Systems
Milton Packer, Christopher S. S. Wilcox, Jeffrey M. M. Testani
Summary: SGLT2 inhibitors have complex effects on renal tubule function and volume homeostasis, with limited diuretic and natriuretic effects. However, they may result in durable improvement of the internal set point for volume homeostasis. These drugs also exert cardioprotective effects through direct actions on cardiomyocytes. The interplay between changes in volume set point and the direct cellular effects may contribute to the benefits of SGLT2 inhibitors in heart failure.
Review
Biochemistry & Molecular Biology
Minyoung Youn, Jesus Omar Gomez, Kailen Mark, Kathleen M. Sakamoto
Summary: RSKs are a group of serine/threonine kinases functioning downstream of the Ras/Raf/MEK/ERK signaling pathway, phosphorylating various targets to regulate cellular processes. In hematological malignancies like AML, overexpression and aberrant activation of RSK isoforms may lead to poor outcomes and chemotherapy resistance.
Article
Pediatrics
Anita Mandal, Priyanka Khandelwal, Thenral S. Geetha, Sakthivel Murugan, Jitendra Meena, Manisha Jana, Aditi Sinha, Rajeev Kumar, Amlesh Seth, Pankaj Hari, Arvind Bagga
Summary: This study evaluated the metabolic and genetic abnormalities in children with nephrolithiasis in North India. The most common metabolic abnormalities were hypercalciuria and hyperoxaluria, and a monogenic etiology was identified in some patients. These findings suggest that a combination of metabolic and genetic evaluation is useful for determining the etiology of nephrolithiasis.
INDIAN JOURNAL OF PEDIATRICS
(2022)
Review
Pediatrics
Flavia Cristina Carvalho Mrad, Silvia Bouissou Morals Soares, Luiz Alberto Wanderley de Menezes Silva, Pedro Versiani dos Anjos Menezes, Ana Cristina Simoes-e-Silva
Summary: Bartter's syndrome is a rare group of salt losing tubulopathies caused by impaired transport mechanisms at the thick ascending limb of the Henle's loop. Different subtypes of BS have been identified, each caused by mutations on specific genes. Clinical and biochemical alterations in BS include polyuria, dehydration, electrolytic disturbances and failure to thrive. Early diagnosis and treatment are crucial to prevent long-term consequences in BS patients.
WORLD JOURNAL OF PEDIATRICS
(2021)
Article
Urology & Nephrology
Nancy S. Krieger, John Asplin, Ignacio Granja, Luojing Chen, Daiana Spataru, Tong Tong Wu, Marc Grynpas, David A. Bushinsky
Summary: In this study, the effects of potassium citrate and chlorthalidone on calcium oxalate stone formation and bone quality were investigated using a genetic hypercalciuric stone-forming rat model. The results showed that potassium citrate plus chlorthalidone combination was more effective in reducing stone formation, while chlorthalidone alone improved bone quality.
KIDNEY INTERNATIONAL
(2021)
Article
Cardiac & Cardiovascular Systems
Petter Bjornstad, Peter J. Greasley, David C. Wheeler, Glenn M. Chertow, Anna Maria Langkilde, Hiddo J. L. Heerspink, DanieL H. Van Raalte
Summary: This study reviews the effects of SGLT2 inhibitors on chronic kidney disease and heart failure, primarily through their diuretic effects. It also suggests the intriguing possibility that SGLT2 inhibitors may modulate nonosmotic sodium storage, providing new insights into mitigating heart failure risk.
JOURNAL OF CARDIAC FAILURE
(2021)
Article
Urology & Nephrology
Muhammad G. Arnous, Jennifer Arroyo, Andrea G. Cogal, Franca Anglani, Hee Gyung Kang, David Sas, Peter C. Harris, John C. Lieske
Summary: This study retrospectively analyzed the clinical and genetic factors of 162 DD1 patients and found that truncating variants associated with the CLCN5 gene were closely related to kidney stone formation and progression of chronic kidney disease in DD1 patients, while nontruncating variants were not associated with microproteinuria and nephrocalcinosis. As for kidney failure, only truncating variants were associated with it. The severity of the disease in patients may be related to the degree of residual ClC-5 function.
KIDNEY INTERNATIONAL REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Rui Hu, Anita Layton
Summary: The study investigates how kidney function is altered in patients with diabetes and the renal effects of an anti-hyperglyceamic therapy that inhibits SGLT2. Results indicate that SGLT2 inhibition may attenuate glomerular hyperfiltration and reduce single-nephron glomerular filtration rate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Norma E. Guerra Hernandez, Laura I. Escobar Perez, Dora Aguilera, Maria Dolores Camargo-Muniz, Cinthya Fabiola Cecena Espinosa, Maria de la Cruz Ruiz Jaramillo, Carolina Salvador, Zinaeli Lopez Gonzalez, Marguerite Hureaux, Rosa Vargas-Poussou
Summary: In this study, clinical and genetic analysis was performed on nine patients from unrelated non-consanguineous Mexican families. Loss-of-function variants in the SLC12A1, KCNJ1, and CLCNKB genes were identified in four patients with Antenatal Bartter syndrome (ABS) and in two patients with Classic Bartter syndrome (CBS). Rating: 8 out of 10.
ARCHIVES OF MEDICAL RESEARCH
(2023)
Article
Genetics & Heredity
Bongmin Bae, Pedro Miura
Summary: Alternative cleavage and polyadenylation (APA) is prevalent in more than 70% of human and mouse genes, with distal poly(A) site selection influencing thousands of genes in the nervous system. Using mouse embryonic stem cell-derived neurons as a model system, researchers demonstrated widespread 3' UTR lengthening that closely resembles APA patterns in mouse cortex. CRISPR/Cas9 editing allows for efficient elimination of long 3' UTR isoform expression, with Oxford Nanopore Technologies platform providing a cost-effective strategy to confirm specific loss of targeted isoforms.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Zikai Liu, Qing Cheng, Xiaoli Ma, Mingke Song
Summary: Pharmacological modulation of the Na+/Ca2+ exchanger (NCX) can suppress melanoma cells and induce cell death, making NCX a potential drug target for cancer therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Kumar Reddy Kakularam, Felix Karst, Aparoy Polamarasetty, Igor Ivanov, Dagmar Heydeck, Hartmut Kuhn
Summary: This study evaluated the specificity of 13 commonly used ALOX inhibitors against human and mouse ALOX isoforms, finding that most inhibitors did not show strict paralog specificity but exhibited interesting ortholog specificity. Care must be taken when interpreting the biological effects of these inhibitors in complex in vitro and in vivo systems due to the variable isoform specificities of the inhibitors.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Cardiac & Cardiovascular Systems
Jan Biegus, Marat Fudim, Husam M. M. Salah, Hiddo J. L. Heerspink, Adriaan A. A. Voors, Piotr Ponikowski
Summary: Congestion is a key feature of heart failure syndrome and achieving effective decongestion is an important therapeutic target. Sodium-glucose cotransporter-2 (SGLT-2) inhibitors have shown clinical benefits in heart failure patients, including reducing the risk of acute episodes. The potential decongestive mechanisms of SGLT-2 inhibitors, such as osmotic diuresis and facilitation of interstitial drainage, contribute to effective and safe decongestion. This review provides a comprehensive analysis of the clinical data on SGLT-2 inhibitor use in acute heart failure.
EUROPEAN JOURNAL OF HEART FAILURE
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)