Journal
APPLICATIONS IN PLANT SCIENCES
Volume 2, Issue 10, Pages -Publisher
BOTANICAL SOC AMER INC
DOI: 10.3732/apps.1400070
Keywords
454 pyrosequencing; Cross Timbers; old-growth forest; post oak; Quercus stellata; simple sequence repeat
Categories
Funding
- Office of Research and Creative Activities at Brigham Young University
Ask authors/readers for more resources
Premise of the study: The American Cross Timbers forest ecosystem runs from southeastern Kansas to Central Texas and is primarily composed of post oak (Quercus stellata). This old-growth forest currently occupies only about 2% of its ancestral range. To facilitate genetic research on this species, we developed microsatellite primers specific to post oak from reduced genomic libraries. Methods and Results: Two Q. stellata individuals, sampled from the northern and southern range of the post oak forest, were subject to genomic reduction and 454 pyrosequencing. Bioinformatic analysis identified putative microsatellites from which 12 polymorphic primer sets were screened on three populations. The number of alleles observed ranged from five to 20 across all populations, while observed and expected heterozygosity values ranged from 0.05 to 0.833 and 0.236 to 0.893, respectively, within individual populations. Conclusions: We report the development of microsatellite markers, specifi c to post oak, to aid the study of genetic diversity and population structure of extant forest remnants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available