Journal
APPLICATIONS IN PLANT SCIENCES
Volume 1, Issue 4, Pages -Publisher
WILEY
DOI: 10.3732/apps.1200386
Keywords
hybridization; Malvaceae; microsatellite marker; population structure; Tilia platyphyllos
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Funding
- Government of Thailand
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Premise of the study: Microsatellite markers in the genus Tilia were developed to investigate the genetic variation in T. platyphyllos and its relationship with T. cordata. Methods and Results: Fifteen microsatellite markers were developed using a microsatellite enrichment protocol. Most loci show a high level of polymorphism in two T. platyphyllos populations from France. The number of alleles ranged from one to 15, with a mean of 8.96. The mean observed and expected heterozygosities were 0.71 and 0.70, respectively. Cross-amplification results indicated that 12 out of 15 loci amplified polymorphic loci in 23 species in the genus. Conclusions: These markers will be useful tools for investigating the phylogeography and hybridization of Tilia species.
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