Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Biochemistry & Molecular Biology
Yue Jing, Jian Zhou, Fenghua Guo, Lin Yu, Xiaomeng Ren, Xiushan Yin
Summary: This study found that betaine promotes osteogenic differentiation and inhibits adipogenic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) and human adipose-derived mesenchymal stem cells (hAD-MSCs). Further analysis showed that betaine exerts its effects through pathways such as the PI3K-Akt signaling pathway, cytokine-cytokine receptor interaction, and ECM-receptor interaction. The results contribute to the exploration of betaine as a potential adjunct for MSC therapy.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Cell Biology
Julia Steger, Alison G. Cole, Andreas Denner, Tatiana Lebedeva, Grigory Genikhovich, Alexander Ries, Robert Reischl, Elisabeth Taudes, Mark Lassnig, Ulrich Technau
Summary: Using single-cell RNA sequencing in the cnidarian Nematostella vectensis, researchers have discovered that neurons, stinging cells, and gland cells originate from a common multipotent progenitor population. They also identified the conserved transcription factor gene SoxC as a key regulator of all neuroglandular lineages. Unlike vertebrates, the differentiation of neuroglandular cells in the sea anemone is maintained throughout all life stages and follows the same molecular trajectories.
Article
Neurosciences
Yongtao Zhou, Hongwang Qiao, Lili Liu, Ping Dong, Fangxu Zhu, Jiawen Zhang, Liping Liu, Li Liu
Summary: The study found that miR-21 was significantly down-regulated in osteoporotic mice, significantly upregulated after osteogenic induction of BMSCs, and significantly down-regulated after adipogenic induction. Overexpression of miR-21 can promote osteogenic differentiation of BMSCs.
JOURNAL OF MUSCULOSKELETAL & NEURONAL INTERACTIONS
(2021)
Review
Materials Science, Biomaterials
Mingxin Qiao, Wen Tang, Zhengyi Xu, Xiaoyue Wu, Wei Huang, Zhou Zhu, Qianbing Wan
Summary: Bone defects are a common bone disease caused by accidents, trauma, and tumors. However, the treatment of bone defects remains a challenge. Recent research on bone repair materials has been successful, but there are few studies on repairing bone defects in patients with hyperlipidemia. Gold nanoparticles have shown potential in promoting bone formation and inhibiting fat accumulation, offering a new strategy for dealing with bone defects in hyperlipidemic patients.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Chenglong Wang, Sabine Stoeckl, Shushan Li, Marietta Herrmann, Christoph Lukas, Yvonne Reinders, Albert Sickmann, Susanne Graessel
Summary: This study investigated the effects of extracellular vesicles (EVs) isolated from different stages of human bone marrow mesenchymal stem cells (hBMSCs) on the osteogenic and adipogenic differentiation capacity of naive hBMSCs. The results showed that EVs from late-stage osteogenic differentiated hBMSCs promoted osteogenic potential in naive hBMSCs and inhibited their adipogenic differentiation. Proteomic analysis revealed distinct protein profiles in EVs from osteogenic and naive hBMSCs.
Article
Chemistry, Applied
Na Ren, Xin Yu, Aizhu Wang, Na Liang, Zhichao Feng, Chunhui Sun
Summary: The study demonstrates that ScCl3 can enhance proliferation and osteogenic differentiation of rat bone MSCs while reducing adipogenic differentiation to a certain extent through the Wnt/beta-catenin signaling pathway. Appropriate concentrations of ScCl3 show potential in improving bone remodeling and may lead to new strategies for designing composites for biomedical applications in the future.
JOURNAL OF RARE EARTHS
(2022)
Article
Cell Biology
Maxwell Mai, Shuai Luo, Samantha Fasciano, Timilehin Esther Oluwole, Justin Ortiz, Yulei Pang, Shue Wang
Summary: This study developed an innovative approach that combines live-cell imaging with cutting-edge deep learning techniques to accurately classify cells differentiated into osteogenic and adipogenic lineages. Among the four pre-trained CNN models evaluated, ResNet 50 showed the highest accuracy and AUC in both binary and multi-class classification tasks. The study demonstrated the capability to predict stem cell fate based on morphology changes using a CNN approach.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Anatomy & Morphology
Zhijie Liang, Donglin Huang, Wenhai Nong, Jinping Mo, Dandan Zhu, Mengxin Wang, Maojian Chen, Changyuan Wei, Hongmian Li
Summary: This study aimed to identify the optimal concentrations of A-PRFe to promote adipogenic and osteogenic differentiation of human adipose-derived stem cells (ASCs). Results showed that A-PRFe significantly stimulated ASC proliferation and enhanced adipogenic/osteogenic differentiation and paracrine function at higher concentrations.
Article
Medicine, Research & Experimental
De-Yu Duan, Jing Tang, Hong-Tao Tian, Yang-Yang Shi, Jie Jia
Summary: Adipocyte-derived MVs-miR-148a promotes adipogenic differentiation and suppresses osteogenic differentiation by targeting the Wnt5a/Ror2 pathway.
Article
Biochemistry & Molecular Biology
Milica Jaksic Karisik, Milos Lazarevic, Dijana Mitic, Nadja Nikolic, Maja Milosevic Markovic, Drago Jelovac, Jelena Milasin
Summary: The failure of oral squamous cell carcinoma (OSCC) treatment is often attributed to cancer stem cells (CSCs) which are resistant to therapy. Micro RNA-21 has been found to play a significant role in the development of OSCC. This study aimed to investigate the multipotency of oral CSCs and evaluate the effects of differentiation on stemness, apoptosis, and miRNA expression. The experiments were conducted using a commercially available OSCC cell line (SCC25) and primary OSCC cultures from five patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Xujie Liu, Yakun Wang, Yan He, Xiaofeng Wang, Ranran Zhang, Akash Bachhuka, Rahul Madathiparambil Visalakshan, Qingling Feng, Krasimir Vasilev
Summary: The study reveals that surface chemistry and topography can synergistically influence the morphology, focal adhesion formation, and osteogenic/adipogenic differentiation of human mesenchymal stem cells. This suggests that different material properties can have a significant impact on cell response and highlights the importance of designing functional biomaterials.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Materials Science, Biomaterials
Jing Zheng, Yongtao Wang, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: This study investigated the effects of cell culture medium viscosity on the differentiation of hMSCs with controlled cell morphology using micropatterns. The results showed that viscosity could influence the osteogenic and adipogenic differentiation of hMSCs, and this effect was dependent on cell morphology.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Cell Biology
Chen Wang, Wen Tian, Shou-Ye Hu, Chen-Xi Di, Chang-Yi He, Qi-Long Cao, Ruo-Han Hao, Shan-Shan Dong, Cong-Cong Liu, Yu Rong, Hua-Feng Kang, Tie-Lin Yang, Zhi Yang, Yan Guo
Summary: This study comprehensively characterized the changes in super enhancers (SEs) during the differentiation of human mesenchymal stem cells (hMSCs) into osteoblasts and adipocytes, revealing that SEs undergo more dramatic changes compared to typical enhancers. Lineage-selective SEs were identified and found to play specific roles in the directed differentiation of hMSCs. Additionally, key transcription factors regulated by lineage-selective SEs were shown to form a core regulatory circuitry that controls the fate determination of hMSCs. Furthermore, GWAS SNPs associated with osteoporosis and obesity were found to be significantly enriched in osteoblasts-selective SEs and adipocytes-selective SEs, respectively.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Wouter Bulten, Kimmo Kartasalo, Po-Hsuan Cameron Chen, Peter Strom, Hans Pinckaers, Kunal Nagpal, Yuannan Cai, David F. Steiner, Hester van Boven, Robert Vink, Christina Hulsbergen-van de Kaa, Jeroen van der Laak, Mahul B. Amin, Andrew J. Evans, Theodorus van der Kwast, Robert Allan, Peter A. Humphrey, Henrik Gronberg, Hemamali Samaratunga, Brett Delahunt, Toyonori Tsuzuki, Tomi Hakkinen, Lars Egevad, Maggie Demkin, Sohier Dane, Fraser Tan, Masi Valkonen, Greg S. Corrado, Lily Peng, Craig H. Mermel, Pekka Ruusuvuori, Geert Litjens, Martin Eklund
Summary: The PANDA challenge is the largest histopathology competition to date, aiming to catalyze the development of reproducible AI algorithms for Gleason grading in prostate cancer. The submitted algorithms achieved pathologist-level performance and their diversity and generalization were validated through cross-continental cohorts.
Article
Biotechnology & Applied Microbiology
Anastasiia Mykuliak, Alma Yrjanainen, Antti-Juhana Maki, Arjen Gebraad, Ella Lampela, Minna Kaariainen, Toni-Karri Pakarinen, Pasi Kallio, Susanna Miettinen, Hanna Vuorenpaa
Summary: The vasculature is a crucial physiological element in human tissues, and the formation of perfusable vascular networks is essential for tissue modeling. This study co-cultured endothelial cells and stromal cells in a hydrogel to form three-dimensional vascular networks. Comparing mesenchymal stem/stromal cells (MSCs) derived from bone marrow and adipose tissue, it was found that these cells supported the formation of mature and interconnected microvascular networks-on-a-chip. However, the origin of MSCs influenced the phenotypes of the vascular networks, with BMSCs inducing the formation of fully perfusable microvasculature and ASCs resulting in partially perfusable microvascular networks. The study also showed that BMSCs outperformed ASCs in terms of pericytic characteristics and gene expression related to vasculature maturation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Elena Herbers, Mimmi Patrikoski, Anita Wagner, Riikka Jokinen, Antti Hassinen, Sini Heinonen, Susanna Miettinen, Hilkka Peltoniemi, Eija Pirinen, Kirsi H. Pietilainen
Summary: Mitochondrial dysfunction in white adipose tissue is associated with obesity. Human adipose-derived stromal/stem cells are a useful tool to study the underlying mechanisms of this dysfunction. Existing protocols for adipogenic differentiation alter the phenotype of white adipocytes and have varying effects on mitochondria. This study aims to find a protocol that can more faithfully recapitulate the white adipocyte phenotype observed in human adipose tissue.
STEM CELLS INTERNATIONAL
(2022)
Article
Cell Biology
Arjen Gebraad, Roope Ohlsbom, Juho J. Miettinen, Promise Emeh, Toni-Karri Pakarinen, Mikko Manninen, Antti Eskelinen, Kirsi Kuismanen, Ana Slipicevic, Fredrik Lehmann, Nina N. Nupponen, Caroline A. Heckman, Susanna Miettinen
Summary: This study investigated the cytotoxicity of Melflufen on BMSCs and its effects on BMSC differentiation and the formation of vascular networks. The results showed that Melflufen has significant cytotoxic effects on BMSCs and affects their differentiation, which could potentially impact therapy outcomes.
Article
Biochemical Research Methods
Philippe Weitz, Yinxi Wang, Kimmo Kartasalo, Lars Egevad, Johan Lindberg, Henrik Gronberg, Martin Eklund, Mattias Rantalainen
Summary: This study proposes a new approach to predict gene expression profiles based on the morphological features of tumors. By using convolutional neural networks, gene expression profiles were successfully predicted from hematoxylin and eosin-stained images, offering a cost-effective solution for large-scale research studies and molecular diagnostics.
Article
Cell Biology
Lars Egevad, Brett Delahunt, Kenneth A. Iczkowski, Theo van der Kwast, Geert J. L. H. van Leenders, Katia R. M. Leite, Chin-Chen Pan, Hemamali Samaratunga, Toyonori Tsuzuki, Nita Mulliqi, Xiaoyi Ji, Henrik Olsson, Masi Valkonen, Pekka Ruusuvuori, Martin Eklund, Kimmo Kartasalo
Summary: There is evidence that cribriform morphology is associated with a worse prognosis for prostatic adenocarcinoma. This study aimed to investigate the reproducibility of cribriform cancer diagnosis in prostate needle biopsies. A panel of nine prostate pathology experts independently reviewed 304 digitised biopsies and achieved a 2/3 consensus diagnosis of cribriform and noncribriform cancer in 90% of the cases. Strict diagnostic criteria were used to identify cribriform patterns and generate a set of consensus cases for standardization.
Article
Multidisciplinary Sciences
Henrik Olsson, Kimmo Kartasalo, Nita Mulliqi, Marco Capuccini, Pekka Ruusuvuori, Hemamali Samaratunga, Brett Delahunt, Cecilia Lindskog, Emiel A. M. Janssen, Anders Blilie, Lars Egevad, Ola Spjuth, Martin Eklund
Summary: This study demonstrates the use of conformal prediction to detect unreliable predictions in the context of prostate biopsy diagnosis and grading. The results show that conformal prediction can improve the accuracy of AI systems and detect systematic differences in external data, highlighting unreliable predictions.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Laura Hyvari, Sari Vanhatupa, Miina Ojansivu, Minna Kelloniemi, Toni-Karri Pakarinen, Leena Hupa, Susanna Miettinen
Summary: This study explores the regulatory mechanisms of bioactive glass on osteogenic differentiation of human mesenchymal stem cells and reveals the involvement of heat shock protein 27 (HSP27) in this process.
Article
Engineering, Biomedical
Reetta Sartoneva, Inari Lyyra, Maiju Juusela, Vipul Sharma, Heini Huhtala, Jonathan Massera, Minna Kellomaki, Susanna Miettinen
Summary: Surgical treatment of urothelial defects with autologous genital or extragenital tissue grafts is susceptible to complications. Tissue engineering using novel biomaterials such as PLA/PBSu blend could provide new treatment options by supporting growth and differentiation of urothelial cells and smooth muscle cells.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Polymer Science
Antonia Ressler, Roope Ohlsbom, Andreja Zuzic, Arjen Gebraad, Erkka J. Frankberg, Toni-Karri Pakarinen, Hrvoje Ivankovic, Susanna Miettinen, Marica Ivankovic
Summary: According to the biomimetic bone scaffold design paradigm, a scaffold resembling natural bone tissue is needed for bone tissue regeneration. This study aimed to improve the properties of growth factor-free scaffolds by adding collagen into the chitosan matrix. The composite scaffolds based on chitosan, collagen, and Mg, Se, Sr, Zn-substituted CaPs showed highly porous structure and enhanced stability, with good viability, proliferation, and osteogenic differentiation of human bone marrow-derived mesenchymal stem/stromal cells.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Multidisciplinary Sciences
Philippe Weitz, Masi Valkonen, Leslie Solorzano, Circe Carr, Kimmo Kartasalo, Constance Boissin, Sonja Koivukoski, Aino Kuusela, Dusan Rasic, Yanbo Feng, Sandra Sinius Pouplier, Abhinav Sharma, Kajsa Ledesma Eriksson, Leena Latonen, Anne-Vibeke Laenkholm, Johan Hartman, Pekka Ruusuvuori, Mattias Rantalainen
Summary: The analysis of FFPE tissue sections stained with H & E or IHC is crucial for evaluating surgically resected breast cancer specimens. Computational pathology image analysis methods have made significant progress recently, but are limited by the scarcity of public datasets. This publication provides the largest publicly available dataset of matched WSIs of H & E and IHC-stained tissue sections from female breast cancer patients.
Article
Multidisciplinary Sciences
Daniel W. Hagey, Miina Ojansivu, Beklem R. Bostancioglu, Osama Saher, Jeremy P. Bost, Manuela O. Gustafsson, Roberto Gramignoli, Mathias Svahn, Dhanu Gupta, Molly M. Stevens, Andre Goergens, Samir EL Andaloussi
Summary: This study provides important insights into the cellular response to extracellular vesicles (EVs), showing that EV dose has a more significant effect than cell source. At high doses, exocytosis is down-regulated and lysosomal activity is up-regulated. However, at low doses, specific responses based on EV cell source are observed, reflecting the activities of the EV's source cells. Furthermore, immune cell-derived EVs are most associated with recipient cells.
Article
Cell & Tissue Engineering
Alma Kurki, Kaarlo Paakinaho, Markus Hannula, Jari Hyttinen, Susanna Miettinen, Reetta Sartoneva
Summary: Tissue engineering offers a new approach for reconstructing large urethral defects, which currently lack optimal repair methods. Cell-seeded scaffolds, including the novel scPLCL(A2P) scaffold, show promise in preventing urethral stricture and promoting urothelial and stromal tissue regeneration. The scPLCL(A2P) scaffold supports the growth and phenotype maintenance of human urothelial cells (hUC) and human adipose-derived stromal cells (hASC) in both mono- and coculture environments.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Materials Science, Biomaterials
Christine Gering, Jenny Parraga, Hanna Vuorenpaa, Lucia Botero, Susanna Miettinen, Minna Kellomaki
Summary: Four versatile modifications of hydrogels were investigated to study the effects on their viscoelastic properties and biological response. The results showed that gelatin-containing hydrogels had better viscoelasticity and induced stronger cell response compared to NaGG-based hydrogels. The study highlighted the importance of both biochemical cues and viscoelasticity in hydrogel materials.
BIOMATERIALS ADVANCES
(2022)
Meeting Abstract
Cell & Tissue Engineering
VigneshKumar Rangasami, Ganesh Nawale, Susanna Miettinen, Bo Nilsson, Yuji Teramura, Jons P. Hilborn, Oommen Varghese, Oommen P. Oommen
TISSUE ENGINEERING PART A
(2022)
