Article
Immunology
Thu Huyen Nguyen, Huy Hoang Dao, Chau Minh Duong, Xuan-Hung Nguyen, Diem Huong Hoang, Xuan-Hai Do, Trung Quang Truong, Tu Dac Nguyen, Liem Thanh Nguyen, Uyen Thi Trang Than
Summary: In recent years, extracellular vesicles (EVs) secreted by mesenchymal stem cells (MSCs) have shown potential as a cell-free therapy for osteoarthritis (OA). This study investigated the effects of EVs released by cytokine-primed umbilical cord-derived MSCs (UCMSCs) on osteoarthritic chondrocyte physiology. The results showed that EVs from cytokine-primed UCMSCs could enhance chondrocyte migration and potentially induce chondrocyte regeneration. These findings suggest that cytokine priming of UCMSCs can selectively deliver therapeutic effects of EV treatment in OA and chondrocyte-related disorders.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Csaba Matta, Rebecca Lewis, Christopher Fellows, Gyula Diszhazi, Janos Almassy, Nicolai Miosge, James Dixon, Marcos C. Uribe, Sean May, Szilard Poliska, Richard Barrett-Jolley, Janos Fodor, Peter Szentesi, Tibor Hajdu, Aniko Keller-Pinter, Erin Henslee, Fatima H. Labeed, Michael P. Hughes, Ali Mobasheri
Summary: Chondrogenic progenitor cells (CPCs) may have superior chondrogenic potential compared to mesenchymal stem cells (MSCs), with a distinct channelome characteristic, including calcium-dependent potassium channels such as BK channels. The KCNMA1 gene was found to be upregulated in CPCs and associated with BK channel activity, impacting proliferation, gene expression, migration, and calcium events in CPCs. These findings contribute to a better understanding of CPCs and their potential in cartilage regeneration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Oncology
Alpha Raj Mekapogu, Zhihong Xu, Srinivasa Pothula, Chamini Perera, Tony Pang, S. M. Zahid Hosen, Vishnu Damalanka, James Janetka, David Goldstein, Romano Pirola, Jeremy Wilson, Minoti Apte
Summary: Pancreatic cancer is a deadly disease with high mortality rate, and its unique characteristics make it difficult to treat. Pancreatic stellate cells play a crucial role in the progression of the disease by interacting with cancer cells. This study demonstrates that a combined approach using HGF/c-MET inhibitors and chemotherapy effectively reduces tumor volume, EMT and stemness, and eliminates metastasis.
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Biochemistry & Molecular Biology
Fatih Karadeniz, Jung Hwan Oh, Mi Soon Jang, Youngwan Seo, Chang-Suk Kong
Summary: Bone marrow adiposity is a complication in osteoporotic patients, and phytochemicals can alleviate it. This study evaluated the effect of libanoridin on the adipogenic differentiation of bone marrow-derived mesenchymal stromal cells. The results showed that libanoridin inhibited adipogenic differentiation through suppressing MAPK-mediated PPAR gamma signaling. Future studies will further explore the anti-adipogenic effects of libanoridin in vivo and elucidate its action mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Feifei Ni, Tao Zhang, Wanan Xiao, Hong Dong, Jian Gao, YaFeng Liu, Jianjun Li
Summary: The study revealed that IL-18 promotes osteogenic differentiation of hBMSCs through the SLC7A5/c-MYC pathway, suggesting a potential role in fracture healing and providing new treatment strategies for delayed fracture healing after splenectomy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Huanshuai Guan, Ning Kong, Run Tian, Ruomu Cao, Guanzhi Liu, Yiyang Li, Qilu Wei, Ming Jiao, Yutian Lei, Fangze Xing, Peng Tian, Kunzheng Wang, Pei Yang
Summary: Melatonin plays a protective role in osteoporosis at different stages of menopause by promoting osteogenic differentiation in bone marrow mesenchymal stem cells.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Anatomy & Morphology
Jiao Li, Yang Lv, Haoyu Wang, Yang Liu, Junxu Ren, Haiping Wang
Summary: By using lentivirus-mediated FGF-2 gene transfection, the differentiation of rat BMSCs into cardiomyocyte-like cells was enhanced, with higher expression levels of cardiac differentiation markers, and specific features observed in the nucleus and cytoplasm.
Article
Cell Biology
Domenico Aprile, Nicola Alessio, Ibrahim H. Demirsoy, Tiziana Squillaro, Gianfranco Peluso, Giovanni Di Bernardo, Umberto Galderisi
Summary: The study demonstrated that multi-lineage stem cells can be isolated from a mouse model, offering a valid alternative for disease research and cell therapy, and showing potential applications for these cells.
Article
Oncology
Shuchong Mei, Yuan Zhang, Li Yu, Guoan Chen, Fuming Zi
Summary: The study found that there were significant differences in FAP alpha expression between AML patients and healthy donors in BM samples, and BMMSCs can protect AML cells from apoptosis through activating the beta-catenin signaling pathway. FAP alpha plays an important role in the AML BM microenvironment and its increased expression may be a poor prognostic factor for AML patients.
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
I. Heggli, U. Blache, N. Herger, T. Mengis, P. K. Jaeger, R. Schuepbach, N. Farshad-Amacker, F. Brunner, J. G. Snedeker, M. Farshad, O. Distler, S. Dudli
Summary: The study found that the presence of FGF2 during the culture of BMSCs derived from MC1 did not detect pro-fibrotic genes and pathways, and significantly decreased cell-matrix adhesion.
EUROPEAN CELLS & MATERIALS
(2022)
Article
Cell Biology
Huijiao Lv, Tao Wang, Shangkun Zhai, Zhuocheng Hou, Sirui Chen
Summary: Using transcriptomic analysis, we elucidated the transcriptomic changes during the osteogenic differentiation of chicken BMSCs at different time points. DEGs were mainly enriched in cell proliferation pathways during the early stage of osteogenic differentiation and pathways related to TGF-beta signaling during the middle and late stages. This study provides the first insight into the dynamic transcriptome changes during BMSC differentiation into osteoblasts in chickens.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Zhehui Zhu, Weidong Meng, Shizhang Liu
Summary: This study aimed to investigate the effect of metformin on the differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs) into chondrocytes with a hypertrophic phenotype and the related mechanisms. The results showed that metformin inhibited the differentiation of BMSCs into chondrocytes with a hypertrophic phenotype and activated AMPK protein during this process.
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING
(2022)
Article
Orthopedics
Ziwei Luo, Jinqi Xie, Haoxiang Ye, Jie Zhang, Yangping Liu, Chunmei Ma, Jiahui Cao, Hao Pan, Xiaosheng Liu, Xianxi Zhou, Jiechen Kong, Dongfeng Chen, Aijun Liu
Summary: In this study, novel-miR-81 was found to promote the chondrogenic differentiation of BMSCs by inhibiting the expression of Rac2. This provides potential therapeutic targets for BMSCs transplantation to repair cartilage defects.