4.5 Article

Enhanced production of fibrinolytic enzyme by a new Xanthomonas oryzae IND3 using low-cost culture medium by response surface methodology

Journal

SAUDI JOURNAL OF BIOLOGICAL SCIENCES
Volume 26, Issue 2, Pages 217-224

Publisher

ELSEVIER
DOI: 10.1016/j.sjbs.2018.08.029

Keywords

Cardiovascular diseases; Xanthomonas oryzae; Solid-state fermentation; Fibrinolytic enzyme; Response surface methodology; Blood clot lysis

Categories

Funding

  1. Deanship of Scientific Research at King Saud University [RG-1435-071]

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Cardiovascular diseases (CVDs) cause high mortality throughout the world. Existing fibrinolytic agents are highly expensive and have many side effects. Microbial fibrinolytic enzymes are very much considered as novel therapeutic candidate for the treatment of CVDs. Reports on fibrinolytic enzyme from Xanthomonas sp. is lacking. This study reports fibrinolytic enzymes from Xanthomonas oryzae IND3 as it shows hyperactivity on fibrin-agarose plates. This organism utilized various agro-industrial wastes for enzymes production. Among all, cow dung enhanced more enzyme production, hence it was used as the low-cost substrate for statistical optimization of fibrinolytic protease in Solid state fermentation. Response surface methodology was employed to optimize the factors and enhanced yield by 4-fold. The interactions among the variables, viz, sucrose, yeast extract, and pH of the medium were investigated using Central Composite Design (CCD). The predicted fibrinolytic enzyme activity was 2340 U/g, and the observed fibrinolytic enzyme activity was 2294 +/- 12.8 U/g. The fibrinolytic enzyme degraded blood clot in vitro completely. This study is the first report on statistical optimization of fibrinolytic enzyme production in SSF from Xanthomonas sp. The crude extract has immense activity on proteinaceous wastes. The production of fibrinolytic protease using the low-cost substrate could reduce the production cost of enzyme. (C) 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.

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