Journal
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
Volume 20, Issue 2, Pages 205-212Publisher
ELSEVIER
DOI: 10.1016/j.sjbs.2013.02.002
Keywords
Haloarcheon; Natrinema sp.; Halocin; Production; Medium optimization
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Funding
- Ministry of Earth Sciences - Centre for Marine Living Resources and Ecology (CMLRE), Government of India [MOES/10-MLR/2/2007]
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Halobacteria, members of the domain Archaea that live under extremely halophilic conditions, are often considered as dependable source for deriving novel enzymes, novel genes, bioactive compounds and other industrially important molecules. Protein antibiotics have potential for application as preserving agents in food industry, leather industry and in control of infectious bacteria. Halocins are proteinaceous antibiotics synthesized and released into the environment by extreme halophiles, a universal characteristic of halophilic bacteria. Herein, we report the production of halocin (SH10) by an extremely halophilic archeon Natrinema sp. BTSH10 isolated from salt pan of Kanyakumari, Tamilnadu, India and optimization of medium for enhanced production of halocin. It was found that the optimal conditions for maximal halocin production were 42 degrees C, pH 8.0, and 104 h of incubation at 200 rpm with 2% (V/V) inoculum concentration in Zobell's medium containing 3 M NaCl, Galactose, beef extract, and calcium chloride as additional supplements. Results indicated scope for fermentation production of halocin for probable applications using halophilic archeon Natrinema sp. BTSH10. (C) 2013 Production and hosting by Elsevier B.V. on behalf of King Saud University.
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