Article
Biochemistry & Molecular Biology
Murray Stewart
Summary: Although the nuclear envelope separates the nucleus and cytoplasm in eukaryotes, a semipermeable barrier is necessary to allow material exchange between these compartments. This barrier is achieved through nuclear pores, which facilitate the active transport of macromolecules. The diffusion of macromolecules through these pores is impeded by a dense network of unfolded regions of nucleoporins. Additional mechanisms, such as binding to immobile components and active removal, are required to maintain the distinctive compositions of each compartment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Patrick P. Collins, Ronan C. Broad, Krithika Yogeeswaran, Arvind Varsani, Anthony M. Poole, David A. Collings
Summary: The nuclear pore is highly conserved across eukaryotes, as are many of the proteins that make up the pore. The transmembrane nuclear pore proteins GP210 and NDC1 play a role in anchoring the nuclear pore in place. In Arabidopsis, the orthologues of GP210 and NDC1 were investigated through analysis of T-DNA insertional mutants. The results showed that the ndc1/gp210 double mutant displayed a range of phenotypic effects, including nuclear-specific changes in nuclear shape and alterations in nuclear transport.
Article
Oncology
Cen-Shan Lin, Yuyi Liang, Shu-Guang Su, Yin-Li Zheng, Neng Jiang, Jing Zhou, Ying Zhang, Ru Deng, Chun-Hua Wang, Shi-Xun Lu, Yu-Hua Huang, Li-Li Liu, Chao Zhang, Jing-Ping Yun
Summary: The study identified elevated Nup93 expression in HCC tissues, especially in cases with metastasis, and its association with poorer prognosis. Nup93 was found to promote HCC metastasis and proliferation by regulating beta-catenin translocation, while the interaction between Nup93 and beta-catenin, as well as a positive feedback loop involving LEF1 and beta-catenin, further facilitated metastasis and proliferation in HCC. The findings suggest potential therapeutic targets in the LEF1-Nup93-beta-catenin pathway for HCC treatment.
Article
Biochemistry & Molecular Biology
Guillaume Holzer, Paola De Magistris, Cathrin Gramminger, Ruchika Sachdev, Adriana Magalska, Allana Schooley, Anja Scheufen, Birgitt Lennartz, Marianna Tatarek-Nossol, Hongqi Lue, Monika Linder, Ulrike Kutay, Christian Preisinger, Daniel Moreno-Andres, Wolfram Antonin
Summary: Nup50 plays a crucial role in NPC assembly independent of its nuclear transport function. It interacts with Nup153 and MEL28/ELYS through a conserved central region, and its N-terminal fragment stimulates the Ran GTPase guanine nucleotide exchange factor RCC1 to facilitate NPC assembly at the end of mitosis.
Article
Biochemistry & Molecular Biology
Rojapriyadharshini Gandhimathi, Dorothea Pinotsi, Mario Koehler, Jorg Mansfeld, Caroline Ashiono, Tatjana Kleele, Sumit Pawar, Ulrike Kutay
Summary: During mitotic entry, nuclear pore complexes (NPCs) disintegrate and so do the large nuclear and cytoplasmic rings formed by Y-complexes. Y-complex Nups dissociate slower than other Nups during in vitro NPC disassembly and remain associated with mitotic ER membranes. Super-resolution microscopy reveals two types of higher-order assemblies of mitotic Y-complexes: large clusters at kinetochores and small focal ER-associated structures, which refine current models of nuclear reassembly.
Article
Biochemistry & Molecular Biology
Xi Wu, Junyou Han, Changkui Guo
Summary: The nucleus is essential for fundamental biological processes in eukaryotes, and is surrounded by the nuclear envelope with nuclear pore complexes (NPC) that regulate the transport of biological macromolecules. The NPC plays a role in genome organization, adaptation, and evolution. In plants, different NPC subcomponents have distinct defensive functions in immune defense, growth and development, hormone signaling, and temperature response. Understanding the components of the NPC is important for breeding high-quality and broad spectrum resistance crops.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Ramona Juehlen, Birthe Fahrenkrog
Summary: Selective exchange between the cytoplasm and nucleus is essential for normal cell function, and mutations in nuclear pore complex proteins have been linked to human diseases. The number of identified mutations and associated diseases is increasing, with different mutations affecting different organs.
Article
Biology
Jacqueline T. Brown, Alexandra J. Haraczy, Christopher M. Wilhelm, Kenneth D. Belanger
Summary: The N-terminal 200 amino acids of Pom152 are sufficient for localization to the NPC, while alterations in other domains may impact Pom152 targeting or affinity for the NPC.
Article
Biochemistry & Molecular Biology
Yuri Y. Shevelyov
Summary: Heterochromatin is mainly localized at the nuclear periphery by interacting with the nuclear lamina and nuclear pore complexes. Recent studies have shown that these interactions play a crucial role in maintaining its peripheral localization, and there are differences in chromatin interactions with the nuclear envelope in cell populations and individual cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Manuel Rogg, Jasmin Maier, Markus Ehle, Alena Sammarco, Oliver Schilling, Martin Werner, Christoph Schell
Summary: This study established an in vitro model to investigate the pathomechanisms of NUP133-related nucleoporopathies. The loss of NUP133 resulted in nuclear pore disruption, altered podocyte-specific transcriptome, and impaired cellular protrusion generation. Comparative analysis of SRNS-related NUP133 mutations showed mild defects.
Article
Biochemistry & Molecular Biology
Linhua Tai, Guoliang Yin, Fei Sun, Yun Zhu
Summary: The nuclear pore complex (NPC) is a large protein assembly that penetrates the nuclear membrane. Recent breakthroughs in cryo-EM and artificial intelligence-based modeling have allowed for improved understanding of the complex structure of the NPC. This review article summarizes the history and latest advancements in studying the architecture of the NPC, as well as future directions for research.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Arianna Penzo, Benoit Palancade
Summary: In this review, the mechanisms of nuclear pore complex (NPC) construction and its role in regulating molecular exchange between the nucleus and cytoplasm are summarized. The study of NPCs not only sheds light on the biogenesis of these complexes but also provides insight into the formation of multiprotein complexes in general.
Article
Multidisciplinary Sciences
Teun A. P. M. Huijben, Hamidreza Heydarian, Alexander Auer, Florian Schueder, Ralf Jungmann, Sjoerd Stallinga, Bernd Rieger
Summary: Particle fusion in single molecule localization microscopy can improve signal-to-noise ratio, but is limited by structural heterogeneity. The authors demonstrate an unsupervised classification method that can differentiate structurally different DNA origami structures without prior knowledge.
NATURE COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Parisa Nobari, Valerie Doye, Charlene Boumendil
Summary: This article reviews the mechanisms by which nuclear pore complexes (NPCs) and nucleoporins affect transcription and DNA repair, including the role of nucleoporin localization and their effects on chromatin organization and physical properties, as well as the contribution of NPCs to genome stability.
Article
Multidisciplinary Sciences
Eszter E. Najbauer, Sheung Chun Ng, Christian Griesinger, Dirk Goerlich, Loren B. Andreas
Summary: The cohesive FG domains in nuclear pore complexes form a selective permeability barrier, with nanoscopic insight into their interactions hindered by sequence heterogeneity. By using an engineered repetitive sequence and NMR spectroscopy, researchers were able to overcome this challenge and map the dynamics of cohesive interactions. The study found that the FG repeats remained disordered in both phase-separated and soluble states, with slow translation observed in phase-separated domains.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Ikram Mossaid, Birthe Fahrenkrog
Article
Multidisciplinary Sciences
Birthe Fahrenkrog, Valerie Martinelli, Nadine Nilles, Gernot Fruhmann, Guillaume Chatel, Sabine Juge, Ursula Sauder, Danika Di Giacomo, Cristina Mecucci, Juerg Schwaller
Article
Cell Biology
Vincent Duheron, Nadine Nilles, Sylvia Pecenko, Valerie Martinelli, Birthe Fahrenkrog
JOURNAL OF CELL SCIENCE
(2017)
Review
Cell Biology
Nadine Nilles, Birthe Fahrenkrog
Article
Biochemistry & Molecular Biology
Guillaume Chatel, Sachin H. Desai, Alexa L. Mattheyses, Maureen A. Powers, Birthe Fahrenkrog
JOURNAL OF STRUCTURAL BIOLOGY
(2012)
Article
Multidisciplinary Sciences
Muhammad Shoaib, David Walter, Peter J. Gillespie, Fanny Izard, Birthe Fahrenkrog, David Lleres, Mads Lerdrup, Jens Vilstrup Johansen, Klaus Hansen, Eric Julien, J. Julian Blow, Claus S. Sorensen
NATURE COMMUNICATIONS
(2018)
Article
Genetics & Heredity
David Walter, Anja Matter, Birthe Fahrenkrog
Review
Cell Biology
Ramona Juhlen, Birthe Fahrenkrog
HISTOCHEMISTRY AND CELL BIOLOGY
(2018)
Review
Cell Biology
Adelia Mendes, Birthe Fahrenkrog
Editorial Material
Cell Biology
Birthe Fahrenkrog, Amnon Harel
Article
Cell Biology
Adelia Mendes, Ramona Juhlen, Sabrina Bousbata, Birthe Fahrenkrog
Article
Cell Biology
Ikram Mossaid, Guillaume Chatel, Valerie Martinelli, Marcela Vaz, Birthe Fahrenkrog
JOURNAL OF CELL SCIENCE
(2020)
Article
Cell Biology
Marcela Vaz, Birthe Fahrenkrog
Summary: Chromosomal translocations involving the NUP98 gene are frequently found in leukemia, causing changes in nuclear organization and histone modification, which largely depend on the tumor suppressor RB. The study reveals an unexpected interplay between NUP98 fusion proteins and RB, potentially contributing to leukemogenesis.
Review
Biochemistry & Molecular Biology
Ramona Juehlen, Birthe Fahrenkrog
Summary: Selective exchange between the cytoplasm and nucleus is essential for normal cell function, and mutations in nuclear pore complex proteins have been linked to human diseases. The number of identified mutations and associated diseases is increasing, with different mutations affecting different organs.