Article
Engineering, Aerospace
Jianwei Wang, Lin Li, Pingyang Wang, Guanrong Hang, Changbin Zhou
Summary: A normally closed one-shot isolation valve has been developed to isolate high-pressure gaseous propellant in micropropulsion systems. This valve utilizes a hollow-designed starting needle to direct metal debris downstream during the valve opening process. The valve was opened at a pressure of 10.6 MPa with a power of 5.6 W, and its performance was mainly analyzed by the volume flow ratio before and after activation.
AEROSPACE SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Claudia Patricia Durasiewicz, Sophia Thekla Guentner, Philipp Klaus Maier, Wolfgang Hoelzl, Gabriele Schrag
Summary: This study presents a normally open microvalve based on energy-efficient piezoelectric actuation for high closing forces and micromachined valve seat trenches for reliable valve operation for medical applications. Additional valve seat coating and a high force actuator are introduced for further leakage reduction. The microvalve is suitable for the design of safe and reliable microfluidic devices.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Analytical
Joel Dungan, Juanita Mathews, Michael Levin, Valencia Koomson
Summary: In this study, an oligomer stamping technique for selective bonding of normally closed valves is optimized for PDMS devices on glass substrates. The process is quantitatively characterized using contact angle and blister bursting testing measurements, and recommendations are made for plasma treatment conditions, microstamping technique, and valve construction. The glass-PDMS devices demonstrate electrical isolation and sealing capability under a pulsed actuation scheme.
Article
Chemistry, Multidisciplinary
Wei Zhan, Ziwei Liu, Shaokun Jiang, Chunying Zhu, Youguang Ma, Taotao Fu
Summary: In this study, the generation processes of bubbles and droplets in a step-emulsification microfluidic device are compared to show the similarities and differences in the emulsification process. By changing the placement of the microdevice, the effects of buoyancy and gravity on the generation of bubbles and droplets are introduced, and the feedback mechanism of the bubble layer and the effect of droplet accumulation on the emulsification process are clarified.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Zhiwei Zhang, Zhongdong Wang, Fengrui Bao, Mengyu Fan, Shaokun Jiang, Chunying Zhu, Youguang Ma, Taotao Fu
Summary: This study investigates the bubble generation in a step-emulsification microdevice using a two-angle photography method, and establishes a model for predicting bubble size. It is found that the bubble generation mechanism is controlled by different factors depending on the capillary number, and the dynamic contact angle and hydrodynamic feedback play important roles in bubble formation.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Xin Liu, Xun Zhang, Yao Yao, Peijun Shi, Chenyi Zeng, Qiang Zhang
Summary: In this study, a switching strategy based on lambda exonuclease is proposed to build molecular circuits. The switch has time-responsive characteristics and can achieve opposite logic output. A time-delay relay with delayed disconnection function is also developed for quantitative control of outputs. Furthermore, digital square and square root circuits are constructed using cascading switches, demonstrating the versatility of the design.
Article
Chemistry, Analytical
Mao Fukuyama, Lin Zhou, Tetsuo Okada, Kristina Simonova, Mikhail Proskurnin, Akihide Hibara
Summary: The technique of deterministically enriching substances in droplets by controlling water transport has the potential to be a powerful pretreatment method for bioassays in droplet microfluidics.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Zhiwei Zhang, Mengyu Fan, Qianqiao Wang, Huaiyu Li, Chunying Zhu, Youguang Ma, Taotao Fu
Summary: This study focuses on the influence of the resultant force caused by the density difference of the two phases on the droplet generation process in a step-emulsification microfluidic device. Threedimensional visualization is used to observe the characteristics of droplet generation. The study proposes a model for droplet generation and obtains a droplet size prediction formula.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Maximilian Fahland, Rohit Mishra
Summary: The generation of droplets in microfluidic systems is widely used in chemical and biological applications. Both actively and passively driven systems are employed for two-phase immiscible micro-droplet formation. In this study, a CFD method was used to model and simulate step-emulsification of water-in-oil on a centrifugal microfluidics platform. The effects of contact angles, supply channel geometry, and capillary number on droplet formation were investigated using the numerical model and compared to experimental data.
MICROFLUIDICS AND NANOFLUIDICS
(2023)
Article
Chemistry, Analytical
Yanming Xia, Chao Song, Yingchao Meng, Peng Xue, Andrew J. deMello, Quan Gao, Stavros Stavrakis, Shenglin Ma, Xiaobao Cao
Summary: Centrifugal microfluidic platforms integrate and perform complex biological and chemical processes within disc-like substrates and valving using centrifugal forces and the Coriolis effect. Researchers have developed and tested an addressable electrowetting centrifugal (EWC) valve, which can be triggered to open by altering its wettability through an external electric field. The valve demonstrates good performance in multiple operations on different fluid samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Junwei Wang, Simon Hahn, Esther Amstad, Nicolas Vogel
Summary: Double emulsions, such as water-in-oil-in-water droplets, are important material platforms for research and applications. A new simple method using a vortex mixer has been introduced for producing single-core double emulsions with high yield. This method allows the creation of tailored double emulsions with minimal time, cost, effort, and expertise, widening its application to nonspecialized scientific communities.
ADVANCED MATERIALS
(2022)
Article
Pediatrics
Ajaya Kumar Gahan, Suksham Jain, Supreet Khurana, Deepak Chawla
Summary: This study compared the effect of closed versus open endotracheal tube suction on the occurrence of ventilator-associated pneumonia in mechanically ventilated neonates. The results showed that the choice of suction method did not significantly impact the incidence of ventilator-associated pneumonia in a unit with a low incidence of this condition.
EUROPEAN JOURNAL OF PEDIATRICS
(2022)
Article
Engineering, Chemical
Xiyang Liu, Shaokun Jiang, Chunying Zhu, Youguang Ma, Taotao Fu
Summary: This article investigates the formation mechanism of viscoelastic fluid droplets in a step-emulsification microdevice using polyethylene oxide glycerin aqueous solution as the dispersed phase and cyclohexane as the continuous phase. It is found that elasticity has a delaying effect on the necking and pinch-off stages of droplet formation, leading to the beading phenomenon.
Review
Chemistry, Multidisciplinary
Mao Fukuyama
Summary: Micrometer-sized water-in-oil droplets have gained attention as carriers for high-throughput parallel analysis of trace samples. By confining trace amounts of water-soluble samples or reagents within the droplets, reactions and detection can be performed while suppressing diffusion dilution. However, concentration enrichment and purification techniques for microdroplet inclusions are still limited.
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2023)
Article
Chemistry, Multidisciplinary
Sheng Mi, Chunying Zhu, Youguang Ma, Taotao Fu
Summary: This study investigates the bubble formation process in high-viscosity liquids using step-emulsification microdevices and analyzes the flow patterns and interface evolution.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Chang-Ni Lin, Yi-Cheng Tsai, Ching-Cheng Hsu, Yu-Ling Liang, Yi-Ying Wu, Chieh-Yi Kang, Chun-Hong Lin, Pang-Hung Hsu, Gwo-Bin Lee, Keng-Fu Hsu
Summary: Tx-01, a novel aptamer, shows specificity in recognizing ovarian cancer cells and tissues, inhibiting migration and invasion, and suppressing tumor growth through disrupting HSP70/NICD interaction. It acts as a prognostic factor and potential inhibitor in ovarian cancer treatment.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Nanoscience & Nanotechnology
Chi-Chien Huang, Yu-Hsuan Kuo, Yi-Sin Chen, Po-Chiun Huang, Gwo-Bin Lee
Summary: Breast cancer, a commonly diagnosed cancer with high mortality rates, can be detected earlier and with improved survival rates using minimally invasive screening approaches based on extracellular vesicle-encapsulated microRNA biomarkers. An integrated microfluidic system was developed to quantify concentrations of breast cancer biomarkers, automating the process within 5 hours and showing potential as a sensitive platform for early-stage breast cancer diagnosis.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Article
Chemistry, Analytical
Yu-Jen Cheng, Chih-Hung Wang, Keng-Fu Hsu, Gwo-Bin Lee
Summary: In this study, an integrated microfluidic system was developed to automatically capture and quantify the methylation level of cfDNA. The system achieved high capture rate and low limit of quantification, allowing for efficient detection of methylated cfDNA from plasma samples in a short time.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Rishabh Gandotra, Hung-Bin Wu, Priya Gopinathan, Yi-Cheng Tsai, Feng-Chih Kuo, Mel S. Lee, Gwo-Bin Lee
Summary: Periprosthetic joint infections (PJIs) are serious complications that are difficult to diagnose accurately, but a newly developed HNP 1 aptamer and associated assay may help improve diagnostic accuracy and assess treatment responses for PJI.
Article
Chemistry, Analytical
Yu-Hung Cheng, Chih-Hung Wang, Keng-Fu Hsu, Gwo-Bin Lee
Summary: Ovarian cancer is one of the most severe gynecologic cancers with asymptomatic early stages. This study developed an integrated microfluidic chip that can extract cfDNA from plasma, detect and quantify mutations in the OvCa biomarker BRCA1. By automating the cfDNA extraction and qPCR processes, this chip can be used for clinical screening of OvCa-associated mutations.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yi-Sin Chen, Chihchen Chen, Charles Pin-Kuang Lai, Gwo-Bin Lee
Summary: This study developed a membrane-based EV isolation/counting platform that can efficiently isolate and quantify specific EVs from blood. The platform has a limit of detection of 105 EVs/mL, demonstrating high sensitivity for disease diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
You-Ru Jhou, Chih-Hung Wang, Huey-Pin Tsai, Yan-Shen Shan, Gwo-Bin Lee
Summary: An integrated microfluidic platform utilizing real-time reverse-transcription loop-mediated isothermal amplification was developed for detection and quantification of three genes of SARS-CoV-2, providing a useful tool for COVID-19 diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Rishabh Gandotra, To -Wen Chen, Feng-Chih Kuo, Mel S. Lee, Gwo-Bin Lee
Summary: In this study, a new microfluidic platform based on magnetic beads and fluorescent probes was developed to detect α-defensin and human neutrophil protein 1 (HNP 1) in synovial fluid. The method showed high sensitivity and specificity, and could rapidly and accurately detect infection biomarkers within 60 minutes. This compact device with a wide dynamic range has great potential for future diagnostics of periprosthetic joint infection.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Tsung-Han Lu, Nai-Jung Chiang, Yi-Cheng Tsai, Priya Gopinathan, Chien-Jui Huang, Jui-Lin Chang, Hsiu-Chi Tu, Yan-Shen Shan, Shang-Cheng Hung, Gwo-Bin Lee
Summary: Cancer can have devastating consequences and is often difficult to detect in its early stages. A new automated integrated microfluidic system has been developed to improve the diagnosis of bile duct cholangiocarcinoma (CCA), a type of cancer that is challenging to diagnose. This system uses three CCA-specific affinity reagents to detect tumor cells in a small sample of CCA patient bile and can automate the entire detection process in just 80 minutes.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
To-Wen Chen, Rishabh Gandotra, Hwan-You Chang, Mel S. Lee, Feng-Chih Kuo, Gwo-Bin Lee
Summary: This study developed a novel integrated microfluidic system (IMS) for detecting two common periprosthetic joint infection (PJI) biomarkers, alpha defensin human neutrophil peptide 1 (HNP-1) and C-reactive protein (CRP), from synovial fluid (SF). The system automatically carried out a magnetic bead-based one-aptamer-one-antibody assay within 45 minutes on a single chip, enabling simultaneous detection of both biomarkers at specific concentration ranges. It is the first report to utilize these two biomarkers as targets in an on-chip assay, and the IMS showed promising diagnostic accuracy in clinical samples.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Rishabh Gandotra, Feng-Chih Kuo, Mel S. Lee, Gwo-Bin Lee
Summary: This study introduces a paper-based microfluidic chip for the quantitative detection of HNP 1 in synovial fluid, providing a rapid and cost-effective diagnostic tool for periprosthetic joint infections (PJI) cases. The chip demonstrates high sensitivity and selectivity, with a wide dynamic range, making it a promising solution for PJI diagnosis in clinical settings.
ANALYTICA CHIMICA ACTA
(2023)
Proceedings Paper
Engineering, Electrical & Electronic
Chi-Chien Huang, Chia-Yu Sung, Yi-Sin Chen, Keng-Fu Hsu, Gwo-Bin Lee
Summary: This study reports an integrated microfluidic system for automated extraction and quantification of extracellular vesicle-derived microRNA from ovarian cancer. By using specific beads, the capture rate of extracellular vesicles was improved and two microRNA biomarkers were simultaneously detected and quantified, providing a precise and specific diagnostic tool for OvCa.
2022 IEEE 35TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS CONFERENCE (MEMS)
(2022)
Article
Biochemical Research Methods
Chia-Yu Sung, Chi-Chien Huang, Yi-Sin Chen, Keng-Fu Hsu, Gwo-Bin Lee
Summary: The study developed an integrated microfluidic platform for isolating and quantifying ovarian cancer blood biomarkers, demonstrating high sensitivity and accuracy. The system showed promising potential as a valuable tool for ovarian cancer biomarker measurements.
Proceedings Paper
Engineering, Electrical & Electronic
Yu-Jen Cheng, Chih-Hung Wang, Gwo-Bin Lee
Summary: An integrated microfluidic chip was reported for capturing and analyzing methylated cfDNA in plasma, with a detection limit as low as 2 pg/μL and a dynamic range from 2 to 200 pg/μL. The automated process can be completed in 2 hours, indicating potential for early diagnosis and prognosis of ovarian cancer using liquid biopsy.
2021 34TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2021)
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Yi-Da Chung, Yuan-Jhe Chuang, Chang-Ni Lin, Keng-Fu Hsu, Gwo-Bin Lee
Summary: An integrated microfluidic platform has been developed for automating the entire process of OCCC diagnosis, discovering the strong correlation between FXYD2 gene expression and OCCC, and proposing a new protocol for FXYD2 gene detection.
2021 34TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2021)
(2021)
