Article
Chemistry, Analytical
Bohdan Josypcuk, Jan Langmaier, Sofiia Tvorynska
Summary: Silver amalgam screen-printed electrodes (AgA-SPEs) were designed, prepared, and tested as representatives among SPEs for measuring at high negative potentials, showing superior PHE values compared to traditional silver-SPEs. The practical application of AgA-SPEs was demonstrated by analyzing iodine content in table salt, with consistent results as declared by the manufacturer.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Physical
Jagriti Sethi, Ahmed Suhail, Mina Safarzadeh, Anas Sattar, Yinghui Wei, Genhua Pan
Summary: Surface modification with NH2 linker on graphene-based sensor surfaces enhances the sensitivity and specificity for detecting A beta biomarkers. The functionalized sensors show excellent performance in spiked human plasma with high stability and reliability. This novel technique provides a promising approach to improve the detection sensitivity of graphene biosensors for minimally invasive disease diagnosis in biofluids.
Article
Engineering, Electrical & Electronic
Tianyuan Hou, Xianfeng Zhou, Yi Xin, Tingting Lin
Summary: A parallel-stripes electrode pattern was proposed and used to prepare piezoelectric nanofibers sensors, showing higher sensitivity and stability through experiments.
IEEE SENSORS JOURNAL
(2021)
Article
Chemistry, Analytical
Alejandro Fidel Alba, Roberto Fernandez-de Luis, Joseba Totoricaguena-Gorrino, Leire Ruiz-Rubio, Julia Sanchez, Jose Luis Vilas-Vilela, Senentxu Lanceros-Mendez, Francisco Javier Del Campo
Summary: Screen printed graphite electrodes are commonly used in electroanalysis and electrochemiluminescence (ECL) applications. This study investigates the ECL of the Ru(bpy)32+/TPrA system on different graphite electrodes printed from various graphite pastes. The ECL intensity of the graphite electrodes is found to depend on electrode kinetics and wettability, which are influenced by paste composition.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
V. Sunil Kumar, Shekher Kummari, Gaelle Catanante, K. Vengatajalabathy Gobi, Jean Louis Marty, K. Yugender Goud
Summary: A label-free electrochemical impedimetric immunosensor was developed for rapid and sensitive detection of zearalenone (ZEN) using palladium nanoparticle embedded functionalized carbon nanotube-chitosan (SPCE/CS-CNT-Pd) modified electrode. The structure of the nanocomposite was analyzed and verified using X-ray diffraction, transmission electron microscopy, and energy dispersive X-ray spectroscopy. The coexistence of Pd nanoparticles with CNTs enhanced the conductivity and catalytic properties of the transducer surface, as well as improved antibody immobilization. The immunosensor showed high sensitivity and simplicity compared to conventional methods and successfully detected ZEN in corn samples with good recovery values.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Engineering, Biomedical
Andrea Spanu, Alberto Botter, Andrea Zedda, Giacinto Luigi Cerone, Annalisa Bonfiglio, Danilo Pani
Summary: This research presents an optimized screen printing technique for the fabrication of textile electrodes based on PEDOT:PSS directly into stretchable garments, demonstrating good performance for dynamic sEMG detections. The results achieved pave the way for the integration of unobtrusive electrodes, obtained by screen printing of conductive polymers, into technical fabrics for rehabilitation and sport monitoring in dynamic conditions.
IEEE TRANSACTIONS ON NEURAL SYSTEMS AND REHABILITATION ENGINEERING
(2021)
Review
Chemistry, Analytical
Rafael Matias Silva, Alexsandra Dias da Silva, Jessica Rocha Camargo, Bruna Santos de Castro, Lais Muniz Meireles, Patricia Soares Silva, Bruno Campos Janegitz, Tiago Almeida Silva
Summary: Electrochemical sensors consisting of screen-printed electrodes (SPEs) are widely used in different fields due to their inherent characteristics such as miniaturization, cost reduction, low sample consumption, compatibility with portable equipment, and disposability. The performance of SPEs is directly influenced by the quality of the material used for printing and modifying the electrodes. Various carbon nanomaterials, including graphene, carbon nanotubes, carbon black, carbon quantum dots, graphitic carbon nitride, and biochar, have been explored for the preparation and modification of SPEs, enhancing their electrochemical response and sensitivity, as well as allowing the immobilization of biological agents for biosensor manufacturing. This review provides an overview of the current use of carbonaceous nanomaterials in the context of electrochemical SPE sensors and discusses potential future directions.
Review
Materials Science, Multidisciplinary
Raghavv Raghavender Suresh, Muthaiyan Lakshmanakumar, J. B. B. Arockia Jayalatha, K. S. Rajan, Swaminathan Sethuraman, Uma Maheswari Krishnan, John Bosco Balaguru Rayappan
Summary: Screen printing has gained popularity in recent times due to its versatility, economic advantages and ease of use. The technique's simplicity in equipment design and requirement of basic materials make it a preferred choice for mass production of electrodes. Optimizing parameters such as ink rheology and mesh size is crucial for achieving desired outcomes in this additive manufacturing technique.
JOURNAL OF MATERIALS SCIENCE
(2021)
Article
Chemistry, Analytical
Melinda David, Adrian Serban, Teodor Adrian Enache, Monica Florescu
Summary: This study described and compared the performances of commercial screen-printed carbon electrodes with different nanostructured materials for LT4 detection. The presence of carbon nanotubes resulted in the best performance for LT4 detection.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Elena Alberto, Julio Bastos-Arrieta, Clara Perez-Rafols, Nuria Serrano, M. Silvia Diaz-Cruz, Jose Manuel Diaz-Cruz
Summary: A DPV method using SPCEs was developed for the determination of SMX, showing a good detection limit (15 μg/L) and a wide linear range (50-600 μg/L) with good repeatability and reproducibility. The method was successfully applied to the analysis of a spiked tap water sample, demonstrating high reproducibility and good trueness. Additional considerations were made on the pH-dependent electrochemical oxidation of SMX and the role of the free terminal amino group in oxidation.
MICROCHEMICAL JOURNAL
(2023)
Article
Engineering, Electrical & Electronic
Marcus A. Hintermueller, Christina Offenzeller, Bernhard Jakoby
Summary: Microfluidic co-flow viscometers use capacitive measurements instead of optical means to determine interface position, with a device fabricated from simple processes for a fully polymeric microfluidic chip and screen-printed capacitive sensor, eliminating the need for coloring liquids or tracer particles.
IEEE SENSORS JOURNAL
(2021)
Review
Chemistry, Analytical
Giti Paimard, Ehsan Ghasali, Mireia Baeza
Summary: As electrochemical measuring instruments, screen-printed electrodes (SPEs) are simple, inexpensive, and rapid to analyze substances with high reproducibility, sensitivity, and accuracy. The latest research has focused on expanding SPE electrochemical biosensors, such as aptasensors, immunosensors, DNA sensors, and enzymatic biosensors, to identify, distinguish, and quantify various biocompounds. This review discusses the use of different nanomaterials in manufacturing on-screen electrode methods and strategies for stable diagnosis of biorecognition elements in the future.
Article
Chemistry, Analytical
Sheila Hernandez, Lydia Garcia, Martin Perez-Estebanez, William Cheuquepan, Aranzazu Heras, Alvaro Colina
Summary: A new, simple and fast protocol using time-resolved Raman spectroelectrochemistry to generate gold-based SERS substrates and detect melamine is presented in this work. The protocol shows significant figures of merit in a very short experiment time and provides valuable information about the interaction between the molecule and the generated substrate.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Alaine Sanchez, Ahmed Esmail Shalan, Maibelin Rosales, Idoia Ruiz de Larramendi, Francisco Javier del Campo
Summary: This work presents a methodology for developing functional metal-oxide based inks for screen-printing by growing nickel hydroxide on the surface of conducting microparticles using different particle to nickel salt ratios. The resulting particles are used as screen-printing inks for electrodes and test structures. The materials are characterized through various techniques and the screen printed electrodes exhibit capabilities for water oxidation and glucose oxidation, as well as electrochromism and an optical bandgap in the range of 4.06-4.15 eV. The approach is applicable to other metal oxides, expanding the possibilities for screen-printed semiconductors and catalysts.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Chiydem Hyusein, Vessela Tsakova
Summary: Carbon nanofibers and single-walled carbon nanotubes screen printed electrodes were used for Pd-Cu modification. Pd nanoparticles were deposited on the electrodes through electroless process, followed by galvanostatic deposition of copper. The Pd-Cu-modified single-walled carbon nanotubes electrode showed higher reductive currents and better stability for nitrate detection compared to the carbon nanofibers electrode.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Maria G. Sande, Debora Ferreira, Joana L. Rodrigues, Luis D. R. Melo, Dirk Linke, Carla J. Silva, Felismina T. C. Moreira, Maria Goreti F. Sales, Ligia R. Rodrigues
Summary: In this study, new aptamers that bind to the virulence factor Yersinia adhesin A (YadA) of Yersinia enterocolitica were identified using cell-systematic evolution of ligands by exponential enrichment (cell-SELEX). A simple electrochemical biosensor was developed using the most promising aptamer candidate (Apt1) to detect YadA. The biosensor showed a linear range of detection and could potentially be used as a sensitive point-of-care detection system for Y. enterocolitica infections.
Article
Chemistry, Applied
Joana J. Costa, Felismina T. C. Moreira, Susana Soares, Elsa Branda, Nuno Mateus, Victor De Freitas, M. Goreti F. Sales
Summary: This study presents an innovative approach for monitoring astringent polyphenols in beverages using an electrochemical biosensor. The biosensor was constructed by adsorbing salivary alpha-amylase or proline-rich protein onto amined gold screen-printed electrodes. The sensor showed high sensitivity and quantitative monitoring capability for astringent compounds in wine samples.
Article
Chemistry, Analytical
Nadia S. Ferreira, Liliana P. T. Carneiro, Christian Viezzer, Maria J. T. Almeida, Ana C. Marques, Alexandra M. F. R. Pinto, Elvira Fortunato, M. Goreti F. Sales
Summary: This work presents an innovative electrochemical biosensor powered by a passive direct methanol fuel cell (DMFC). The biosensor utilizes a molecularly imprinted polymer (MIP) layer on the anode side of the DMFC to selectively detect sarcosine, a potential cancer biomarker. By serially assembling a stack of hybrid DMFC/biosensor devices, the concentration-dependent detection of sarcosine triggers a color change in an external electrochromic cell (EC), making this biosensor a self-controlled and self-signalling device. This approach offers a simple, low-cost solution for autonomous biosensors, suitable for point-of-care analysis.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Tomas Pinheiro, Ricardo Correia, Maria Morais, Joao Coelho, Elvira Fortunato, M. Goreti F. Sales, Ana C. Marques, Rodrigo Martins
Summary: By introducing aromatic chemical structures and controlling laser fluence, we enhanced the conductivity of paper-based LIG. With these improved properties, we developed a simple transfer method to transfer LIG structures from paper substrates to flexible substrates, providing a solution for multiple applications in wearable electronics.
Article
Engineering, Environmental
Liliana P. T. Carneiro, Alexandra M. F. R. Pinto, M. Goreti F. Sales
Summary: This work presents a paper-based direct methanol fuel cell platform (PB-DMFC) that serves as both an energy source and a biosensor for point-of-care applications. By assembling the fuel cell components on a treated paper strip, a flexible and self-sufficient biosensor is created, capable of detecting sarcosine. The platform demonstrates stable electrical signals and good reproducibility, with a sensing layer built on the anode electrode for sarcosine detection.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Electrochemistry
Luiza A. Wasiewska, Fernando Garrido Diaz, Sofia Rodrigues Teixeira, Catherine M. Burgess, Geraldine Duffy, Alan O'Riordan
Summary: In this study, a silicon-based chip with six interdigitated gold microelectrodes (IDE) sensors was used to develop an amplification-free multiplex electrochemical sensor for the simultaneous detection of stx1 and stx2 genes. The developed sensor showed high selectivity and achieved a lower limit of detection of 100 zM (10-19 M) compared to previous reports. This sensor has the potential to revolutionize the point-of-use detection of Shiga toxin-producing E. coli (STEC) and other pathogens in on-farm or food industry settings.
ELECTROCHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Artur Filipe Rodrigues, Ana P. M. Tavares, Susana Simoes, Rui P. F. F. Silva, Tomas Sobrino, Bruno R. Figueiredo, Goreti Sales, Lino Ferreira
Summary: Graphene-based materials (GBMs) have been explored for developing flexible interfaces to treat neurological disorders by improving brain function and tissue regeneration through electrical stimulation. Recent findings of GBM electrodes generating electrical response under light exposure have inspired the development of non-genetic approaches, such as optogenetics, to selectively modulate brain cells. In this study, graphene was conjugated with upconversion nanoparticles (UCNPs) to enable wireless transcranial activation using near-infrared (NIR) radiation. The optimized UCNP formulations attached to graphene nanoplatelets showed enhanced electrical conductivity upon NIR activation, promoting cell proliferation and downregulating neuronal and glial differentiation markers in human neuroblastoma cells.
Article
Biotechnology & Applied Microbiology
Maria G. G. Sande, Debora Ferreira, Joana L. L. Rodrigues, Luis D. R. Melo, Athanasios Saragliadis, Dirk Linke, Felismina T. C. Moreira, Maria Goreti F. Sales, Ligia R. R. Rodrigues
Summary: In this study, a new point-of-care (PoC) diagnostic platform was developed to detect bacterial infections and address the increasing antimicrobial resistance crisis. By utilizing cell-systematic evolution of ligands by exponential enrichment (cell-SELEX) and next-generation sequencing, new aptamer sequences with high affinity for the ubiquitous surface protein A2 (UspA2) of Moraxella catarrhalis were identified. The best-performing aptamer, Apt1_RC, had a K-d value of 3.4 nM and was functionalized onto a label-free electrochemical biosensor for UspA2 detection.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Silvia Soares, Isabel Faria, Fatima Aires, Armanda Monteiro, Gabriela Pinto, Maria Goreti Sales, Miguel A. Correa-Duarte, Susana G. Guerreiro, Ruben Fernandes
Summary: More than 50% of prostate cancer patients are treated with radiotherapy. Gold nanoparticles (AuNPs) could be used as potential radiosensitizers to overcome the limitations of radiotherapy. This study assessed the biological interaction of different morphologies of AuNPs with ionizing radiation (IR) in prostate cancer cells. The results showed that AuNPs combined with IR decreased cell viability and increased apoptosis, suggesting that AuNPs could improve the efficacy of radiotherapy in prostate cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Carolina S. Hora, Ana P. M. Tavares, Liliana P. T. Carneiro, Dzmitry Ivanou, Adelio M. Mendes, Goreti F. Sales, M. Goreti F. Sales
Summary: This work proposes an integrated power solution, with an autonomous and self-signaling biosensing device, to detect sarcosine, a known biomarker for prostate cancer. The biosensor is assembled on the counter-electrode of a dye-sensitized solar cell (DSSC), using molecular imprinting to produce the biorecognition element. The device is completely equipment-free and suitable for point-of-care analysis.
Article
Cell Biology
Silvia Soares, Claudia Pereira, Andre P. Sousa, Ana Catarina Oliveira, Maria Goreti Sales, Miguel A. Correa-Duarte, Susana G. Guerreiro, Ruben Fernandes
Summary: Nanomaterials have diverse applications in biomedicine, and the shapes of gold nanoparticles can modulate tumor cell behavior. In this study, different shapes of polyethylene glycol coated-gold nanoparticles were synthesized and their impact on metabolic activity and cellular proliferation in prostate cancer cells was evaluated. The results demonstrated that the morphology of AuNPs is an essential modulator of cell behavior and should be carefully considered for their application in nanomedicine.
Article
Pharmacology & Pharmacy
Ana Isabel Sebastiao, Daniela Mateus, Mylene A. Carrascal, Catia Sousa, Luisa Cortes, Martin F. Bachmann, Analia do Carmo, Ana Miguel Matos, Maria Goreti F. Sales, Maria Teresa Cruz
Summary: Dendritic cells (DCs) are specialized antigen-presenting cells that prime antigen-specific T cells. The interaction between DCs and the spike protein of SARS-CoV-2 plays a pivotal role in inducing effective immunity. Virus-like particles (VLPs) containing the receptor-binding motif of the spike protein promoted DC maturation and activation of T cells, suggesting their potential as a vaccine against SARS-CoV-2.
Review
Chemistry, Multidisciplinary
Claudia Pereira, Claudio Parolo, Andrea Idili, Roger R. Gomis, Ligia Rodrigues, Goreti Sales, Arben Merkoci
Summary: Implementing a widespread diagnostic campaign for early cancer detection could save millions of lives and billions of dollars annually. However, current approaches to cancer diagnosis are complex and expensive, limiting their use in low- and middle-income settings. This article explores the technical challenges in achieving precise cancer diagnostics and discusses the potential of paper-based sensors as widely available sensing platforms for cancer detection. The authors also present their vision on how to make paper-based sensors a commonly used diagnostic tool for cancer.
TRENDS IN CHEMISTRY
(2022)
Article
Chemistry, Physical
Ana Claudia Pereira, Andre F. Pina, Diana Sousa, Debora Ferreira, Catia Santos-Pereira, Joana L. Rodrigues, Luis D. R. Melo, Goreti Sales, Sergio F. Sousa, Ligia R. Rodrigues
Summary: The study used the SELEX method to generate and isolate new aptamers targeting CatB, and assessed their binding affinity using bioinformatics tools. Molecular docking studies and simulations identified a new aptamer with potential to target CatB. Cell binding assays confirmed the predictions, validating the newly discovered aptamer as a promising targeting ligand for CatB-overexpressing cancer cells.
MOLECULAR SYSTEMS DESIGN & ENGINEERING
(2022)
Review
Chemistry, Analytical
Matheus Siqueira Silva, Ana P. M. Tavares, Henrique Dipe de Faria, Maria Goreti Ferreira Sales, Eduardo Costa Figueiredo
Summary: This review discusses the rational design and applications of molecularly imprinted polymers (MIPs) in solid phase extraction of disease biomarkers from biological samples, as well as the remaining challenges and future trends in the application of Molecular Imprinting Technology in sample preparation for clinical diagnosis.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Biomaterials
Izabela Stupka, Artur P. Biela, Bernard Piette, Agnieszka Kowalczyk, Karolina Majsterkiewicz, Kinga Borzecka-Solarz, Antonina Naskalska, Jonathan G. Heddle
Summary: Artificial protein cages, such as TRAP-cages, have potential applications in vaccines and drug delivery. TRAP-cages have the ability to control the disassembly conditions by modifying the interface between their building blocks. By using TRAP rings with different numbers of monomers, it is possible to predict the formation of other cages.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Guo Zhang, Yu Wang, Hua Qiu, Lei Lu
Summary: This study presents a one-pot synthesis method for flower-like AMPs@EAMP particles by combining antimicrobial peptides with ellagic acid, offering enlarged surface area, excellent biocompatibility, and broad-spectrum antibacterial activity. In vivo studies indicate their potential for tissue repair and immune barrier reconstruction.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Jiamei Zhang, Lingshuang Wang, Cheng Xu, Yingui Cao, Shengsheng Liu, Rui L. Reis, Subhas C. Kundu, Xiao Yang, Bo Xiao, Lian Duan
Summary: Pluronic F127 modified silk fibroin film with different types of antibacterial agents could accelerate wound recovery by promoting fibroblast adhesion, eradicating bacteria, and facilitating angiogenesis and re-epithelialization.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Yinsheng Liu, Mingyue Wang, Yinfei Hui, Lei Sun, Yanrui Hao, Henlong Ren, Hao Guo, Wu Yang
Summary: In this study, a rare-earth hybrid luminescent material was developed for the detection of a biomarker for anthrax. The material showed excellent selectivity and high sensitivity, allowing for the determination of the biomarker in saliva and urine. Additionally, a convenient point-of-care testing method using fluorescent test paper and a smartphone was established for the initial diagnosis of anthrax.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Review
Materials Science, Biomaterials
Wenshuai Yang, Jingsi Chen, Ziqian Zhao, Meng Wu, Lu Gong, Yimei Sun, Charley Huang, Bin Yan, Hongbo Zeng
Summary: Injectable hydrogels with shear-thinning and/or in situ formation properties offer distinct advantages in bioengineering applications, as they can be directly delivered to target sites, possess self-healing abilities, and simplify the implantation process.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)