Article
Immunology
Julia Harwardt, Jan P. Bogen, Stefania C. Carrara, Michael Ulitzka, Julius Grzeschik, Bjoern Hock, Harald Kolmar
Summary: In this study, a Two-in-One antibody was identified for the first time through yeast surface display screening. The antibody can simultaneously target EGFR and PD-L1, and has a dual action by inhibiting EGFR signaling and blocking the PD-1/PD-L1 interaction. This research provides a new strategy for the development of therapeutic antibodies with novel binding properties.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Zijun Bie, Yang Chen
Summary: An IFN-alpha specific plastic antibody was prepared using boronate affinity oriented surface imprinting, showing strong affinity and high specificity for selective enrichment of IFN-alpha. This plastic antibody has potential applications in the pharmaceutical industry.
Article
Chemistry, Multidisciplinary
Markus Goethel, Martin Listek, Katrin Messerschmidt, Anja Schloer, Anja Hoenow, Katja Hanack
Summary: This study combined bioinformatic analyses and novel immunization techniques to design monoclonal antibodies for the detection of whole microorganisms, successfully targeting E. coli O157:H7.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Analytical
He Chen, Yuan Ding, Jiao Li, Lianrun Huang, Gualberto Gonzalez-Sapienza, Bruce D. Hammock, Minghua Wang, Xiude Hua
Summary: This study presents a new unconstrained ratiometric signal approach by integrating negative-readout competitive and positive-readout noncompetitive immunoassays into a single assay. The method has been demonstrated to be applicable to fluorescence and colorimetry readouts, and exhibits strong anti-interference ability to external light environment.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
John P. Giddens, John E. Schiel
Summary: This study describes the development and application of a strategy to generate functionally relevant analytical challenge materials with unique site-specific attributes. By using a ligand bound to the antibody during oxidative stress, unique oxidation patterns with site-specific modifications were generated. The study demonstrates rapid detection of antibody oxidation using surface plasmon resonance assay and qualitative thermal melting.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Guillaume Lefebvre, Antoine Maze, Rosa Alvarez-Palencia Jimenez, Franz Bruckert, Vasco Filipe, Sylvain Huille, Marianne Weidenhaupt
Summary: This study investigates the impact of plastic material surface properties on surfactant and monoclonal antibody adsorption. Results show that Polysorbate 80 provides significantly better protection against monoclonal antibody adsorption on polystyrene surfaces compared to hexadecane self-assembled monolayers, with protection efficacy depending on the concentration of surfactants and antibodies. The nature of plastic polymer surfaces affects surfactant adsorption and influences their protection efficacy in therapeutic antibody formulations, beyond just hydrophobicity.
PHARMACEUTICAL RESEARCH
(2021)
Article
Nanoscience & Nanotechnology
Meiyun Xu, Lei Zhou, Linjuan Zhang, Shitong Zhang, Fulong Chen, Ruhong Zhou, Daoben Hua
Summary: This study adopts a two-dimensional imprinting strategy to design 2D imprinted networks with specific nanotraps for effective uranium capture. The as-prepared adsorbents possess tailored 2D nanotraps open and specific to uranyl, showing excellent selectivity, high adsorption capacity, and good regeneration performance for uranium. This pioneering strategy of 2D imprinting networks has significant implications for material design in various fields.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Chemistry, Analytical
Elly Septia Yulianti, Siti Fauziyah Rahman, Yudan Whulanza
Summary: Cortisol, a steroid hormone, is closely related to the stress response and can be used as a biomarker for early stress detection. An electrochemical immunosensor, with antibodies as its bioreceptor, is a widely used method for cortisol detection. However, both antibodies and aptamers face issues with short shelf life. Molecularly imprinted polymers (MIPs) provide an alternative to natural antibodies, offering high durability, long shelf life, and the ability to detect cortisol molecules at a low concentration.
Article
Biochemistry & Molecular Biology
Jisoo Park, Sangwoo Lee, Yuseon Kim, Tae Hyeon Yoo
Summary: Antibody-protein conjugates are valuable tools for studying biological systems and advancing therapeutics and diagnostics. Challenges in generating bispecific antibodies and antibody-enzyme conjugates can be addressed through enzymatic and chemical approaches, offering solutions for site-specific conjugation of antibodies with other proteins.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Zhanchen Guo, Rongrong Xing, Menghuan Zhao, Ying Li, Haifeng Lu, Zhen Liu
Summary: The study introduces a novel approach called ROSIC for engineering nanoparticles with specific targeting capability toward proteins and peptides. Using engineered coreless imprinted and cladded silica nanoparticles, the study demonstrates high specificity and high affinity for protein targeting. The approach successfully achieves targeted imaging of triple-negative breast cancer cells and mice, showing great promise for biomedical applications.
Article
Business
Shuangfa Huang, Martina Battisti, David Pickernell
Summary: This study uses fsQCA to examine how venture strategy, founding team knowledge diversity, and demographic diversity interact to explain revenue growth of new ventures. Based on a longitudinal dataset of 210 new ventures, we find that the effects of team diversity are complex, with different conditions explaining short-term and sustained growth, contingent on the venture's strategy. We identify different recipes for short-term and long-term revenue growth, suggesting the potential role of imprinting and an innovation strategy. The findings provide insights into the relative relevance of innovation strategy, knowledge diversity, and demographic diversity at distinct stages of venture founding.
JOURNAL OF BUSINESS RESEARCH
(2023)
Article
Chemistry, Analytical
Tongtong Hou, Yue Huang, Xin Wang, Xiaoling Hu, Ping Guan
Summary: In this study, lysozyme-imprinted mesoporous Zr-based MOF (mesoUiO-66-NH2@MIPs) with specific recognition of lysozyme (Lyz) was prepared using surface imprinting technology. The mesoUiO-66-NH2@MIPs showed excellent hydrophilicity and high loading capacity for macromolecular protein Lyz, and could specifically recognize Lyz from mixed protein systems.
Article
Biophysics
Yu Yang, Chuan Zeng, Jing Huang, Mengfan Wang, Wei Qi, Haixia Wang, Zhimin He
Summary: In this study, a surface cell imprinted SERS mapping platform was developed for specific and quantitative detection of bacteria. The platform exhibited excellent specificity towards Escherichia coli (E. coli) and demonstrated good quantitative ability with a wide linear range and low detection limit. Furthermore, the platform proved to be effective in detecting E. coli in real samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Immunology
Shengli Song, Miriam Manook, Jean Kwun, Annette M. Jackson, Stuart J. Knechtle, Garnett Kelsoe
Summary: Antibody-mediated allograft rejection is a major cause of kidney transplant failure, with research focusing on identifying antibodies and optimizing therapies. New reagents and techniques have been used to measure MHC sensitization and isolate HLA-specific B cells from sensitized patients.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Engineering, Multidisciplinary
Jun Qian, Xiaoqian Xu, Jiajia Su, Weihuan Zeng, Bing Han, Xiaolong Hao, Guoyin Kai
Summary: This study reported a strategy for effective adsorption of Salvianolic acid A (SAA) from aqueous solution using surface molecularly imprinted magnetic nanoparticles (SIMNP). By selecting appropriate functional monomers, high-efficiency and highly selective adsorption of SAA was achieved in a short period of time, providing a new approach for the effective capture of SAA.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Materials Science, Biomaterials
Zahid Ullah Khan, Mayara Klimuk Uchiyama, Latif Ullah Khan, Koiti Araki, Hiro Goto, Maria Claudia Franca Cunha Felinto, Ana Olivia de Souza, Hermi Felinto de Brito, Magnus Gidlund
Summary: The study focused on the development of water-dispersible Eu3+/Mn2+ co-doped ZnSe@ZnS core/shell QDs as bionanoprobes for biomedical applications, evaluating their biocompatibility, fluorescence behavior, and cellular uptake and exocytosis. By co-incorporating Eu3+/Mn2+ codopants, the QDs exhibited tunable emission colors and enhanced emission intensity, with high cell viability and no cell activation observed. The QDs were internalized by cells predominantly through macropinocytosis and lipid raft-mediated endocytic pathways, showing color-tunable fluorescence and increasing intensity over time.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Review
Biochemistry & Molecular Biology
Diana A. Sousa, Mariana Carneiro, Debora Ferreira, Felismina T. C. Moreira, Maria Goreti F. Sales, Ligia R. Rodrigues
Summary: Early diagnosis of cancer can be significantly improved by using specific nucleic acid aptamers. These aptamer-based biosensors have high affinity and specificity, and are used in various cancer detection strategies.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Electrochemistry
Carolina Hora, Fatima Santos, Ana M. V. M. Pereira, M. Goreti F. Sales, Dzmitry Ivanou, Adelio Mendes
Summary: Dye-sensitized solar cells (DSSC) are a promising technology for indoor photovoltaics in powering wireless sensor nodes for Internet of Things (IoTs). This study presents a new PEDOT:PSS (PP) and graphene nano platelets (GNPs) composite layer as a counter-electrode for Co(III/II) mediated DSSCs, which exhibits higher electrochemical activity and protruding morphology. Despite the difference in catalytic activity, the PP:GNP counter-electrode shows comparable power conversion efficiency (PCE) to pristine PP in traditional DSSCs, but outperforms PP under artificial light.
ELECTROCHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Ana Margarida L. Piloto, David S. M. Ribeiro, S. Sofia M. Rodrigues, Joao L. M. Santos, Paula Sampaio, Maria Goreti Ferreira Sales
Summary: In this study, molecularly imprinted polymers (MIPs) were successfully assembled around quantum dots (QDs) for the detection of the protein biomarker CA19-9 associated with pancreatic cancer (PC). The imprinted cellulose hydrogel exhibited fluorescence quenching with increasing concentrations of CA19-9, showing linearity in a range that is clinically significant. The MIPs conjugated to QDs increased the sensitivity of the system for optical detection, making it a potential candidate for sensitive and low-cost point-of-care tests (PoCTs).
Review
Chemistry, Analytical
Mariana C. C. G. Carneiro, Ligia R. Rodrigues, Felismina T. C. Moreira, Maria Goreti F. Sales
Summary: This review discusses the potential of using paper-based sensors in the early diagnosis, screening, and treatment of cancer. Paper sensors offer low-cost and disposable devices with colorimetric detection for cancer biomarkers. However, the main challenges with these devices are low sensitivity and inefficiency in quantitative measurements.
Article
Chemistry, Analytical
Carla F. Pereira, M. Goreti F. Sales, Manuela F. Frasco
Summary: This study reports a label-free sensor for accurate and timely diagnosis of venous thromboembolism (VTE) by using a photonic molecularly imprinted polymer (PMIP) that shows linear response to D-dimer in synthetic urine and selectively recognizes D-dimer in the presence of fibrinopeptide B.
Article
Engineering, Environmental
Liliana P. T. Carneiro, Alexandra M. F. R. Pinto, M. Goreti F. Sales
Summary: This work presents a paper-based direct methanol fuel cell platform (PB-DMFC) that serves as both an energy source and a biosensor for point-of-care applications. By assembling the fuel cell components on a treated paper strip, a flexible and self-sufficient biosensor is created, capable of detecting sarcosine. The platform demonstrates stable electrical signals and good reproducibility, with a sensing layer built on the anode electrode for sarcosine detection.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Artur Filipe Rodrigues, Ana P. M. Tavares, Susana Simoes, Rui P. F. F. Silva, Tomas Sobrino, Bruno R. Figueiredo, Goreti Sales, Lino Ferreira
Summary: Graphene-based materials (GBMs) have been explored for developing flexible interfaces to treat neurological disorders by improving brain function and tissue regeneration through electrical stimulation. Recent findings of GBM electrodes generating electrical response under light exposure have inspired the development of non-genetic approaches, such as optogenetics, to selectively modulate brain cells. In this study, graphene was conjugated with upconversion nanoparticles (UCNPs) to enable wireless transcranial activation using near-infrared (NIR) radiation. The optimized UCNP formulations attached to graphene nanoplatelets showed enhanced electrical conductivity upon NIR activation, promoting cell proliferation and downregulating neuronal and glial differentiation markers in human neuroblastoma cells.
Article
Engineering, Biomedical
Ana R. Cardoso, Joao Frederico Alves, Manuela F. Frasco, Ana Margarida Piloto, Veronica Serrano, Daniela Mateus, Ana Isabel Sebastia, Ana Miguel Matos, Analia Carmo, Teresa Cruz, Elvira Fortunato, M. Goreti F. Sales
Summary: This work presents an innovative ultra-sensitive biosensor with Spike protein for monitoring SARS-CoV-2 antibodies. The biosensor demonstrated a high sensitivity and accuracy in capturing antibodies in serum using electrochemical impedance spectroscopy. The ability to detect antibodies at low concentrations makes it a promising tool for assessing protective immunity and guiding revaccination.
MATERIALS TODAY BIO
(2022)
Article
Biotechnology & Applied Microbiology
Maria G. G. Sande, Debora Ferreira, Joana L. L. Rodrigues, Luis D. R. Melo, Athanasios Saragliadis, Dirk Linke, Felismina T. C. Moreira, Maria Goreti F. Sales, Ligia R. R. Rodrigues
Summary: In this study, a new point-of-care (PoC) diagnostic platform was developed to detect bacterial infections and address the increasing antimicrobial resistance crisis. By utilizing cell-systematic evolution of ligands by exponential enrichment (cell-SELEX) and next-generation sequencing, new aptamer sequences with high affinity for the ubiquitous surface protein A2 (UspA2) of Moraxella catarrhalis were identified. The best-performing aptamer, Apt1_RC, had a K-d value of 3.4 nM and was functionalized onto a label-free electrochemical biosensor for UspA2 detection.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Silvia Soares, Isabel Faria, Fatima Aires, Armanda Monteiro, Gabriela Pinto, Maria Goreti Sales, Miguel A. Correa-Duarte, Susana G. Guerreiro, Ruben Fernandes
Summary: More than 50% of prostate cancer patients are treated with radiotherapy. Gold nanoparticles (AuNPs) could be used as potential radiosensitizers to overcome the limitations of radiotherapy. This study assessed the biological interaction of different morphologies of AuNPs with ionizing radiation (IR) in prostate cancer cells. The results showed that AuNPs combined with IR decreased cell viability and increased apoptosis, suggesting that AuNPs could improve the efficacy of radiotherapy in prostate cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Carolina S. Hora, Ana P. M. Tavares, Liliana P. T. Carneiro, Dzmitry Ivanou, Adelio M. Mendes, Goreti F. Sales, M. Goreti F. Sales
Summary: This work proposes an integrated power solution, with an autonomous and self-signaling biosensing device, to detect sarcosine, a known biomarker for prostate cancer. The biosensor is assembled on the counter-electrode of a dye-sensitized solar cell (DSSC), using molecular imprinting to produce the biorecognition element. The device is completely equipment-free and suitable for point-of-care analysis.
Article
Cell Biology
Silvia Soares, Claudia Pereira, Andre P. Sousa, Ana Catarina Oliveira, Maria Goreti Sales, Miguel A. Correa-Duarte, Susana G. Guerreiro, Ruben Fernandes
Summary: Nanomaterials have diverse applications in biomedicine, and the shapes of gold nanoparticles can modulate tumor cell behavior. In this study, different shapes of polyethylene glycol coated-gold nanoparticles were synthesized and their impact on metabolic activity and cellular proliferation in prostate cancer cells was evaluated. The results demonstrated that the morphology of AuNPs is an essential modulator of cell behavior and should be carefully considered for their application in nanomedicine.
Article
Pharmacology & Pharmacy
Ana Isabel Sebastiao, Daniela Mateus, Mylene A. Carrascal, Catia Sousa, Luisa Cortes, Martin F. Bachmann, Analia do Carmo, Ana Miguel Matos, Maria Goreti F. Sales, Maria Teresa Cruz
Summary: Dendritic cells (DCs) are specialized antigen-presenting cells that prime antigen-specific T cells. The interaction between DCs and the spike protein of SARS-CoV-2 plays a pivotal role in inducing effective immunity. Virus-like particles (VLPs) containing the receptor-binding motif of the spike protein promoted DC maturation and activation of T cells, suggesting their potential as a vaccine against SARS-CoV-2.
Article
Nanoscience & Nanotechnology
Zahid U. Khan, Latif U. Khan, Mayara K. Uchiyama, Fernanda M. Prado, Rodrigo L. Faria, Israel F. Costa, Sayuri Miyamoto, Koiti Araki, Magnus Gidlund, Hermi F. Brito, Paolo Di Mascio
Summary: In biomedical sciences, it is important to utilize multifunctional nanoparticles of similar size that have tunable emission. This study developed a method using interfacial ion exchange to synthesize color-tunable CdSe/ZnS quantum dots (QDs). The QDs showed wide-range color-tunability without a significant change in size. The study also evaluated the biocompatibility, bioimaging, and delivery pathways of the QDs for future photodynamic therapy.
ACS APPLIED NANO MATERIALS
(2023)
Article
Materials Science, Biomaterials
Izabela Stupka, Artur P. Biela, Bernard Piette, Agnieszka Kowalczyk, Karolina Majsterkiewicz, Kinga Borzecka-Solarz, Antonina Naskalska, Jonathan G. Heddle
Summary: Artificial protein cages, such as TRAP-cages, have potential applications in vaccines and drug delivery. TRAP-cages have the ability to control the disassembly conditions by modifying the interface between their building blocks. By using TRAP rings with different numbers of monomers, it is possible to predict the formation of other cages.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Guo Zhang, Yu Wang, Hua Qiu, Lei Lu
Summary: This study presents a one-pot synthesis method for flower-like AMPs@EAMP particles by combining antimicrobial peptides with ellagic acid, offering enlarged surface area, excellent biocompatibility, and broad-spectrum antibacterial activity. In vivo studies indicate their potential for tissue repair and immune barrier reconstruction.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Jiamei Zhang, Lingshuang Wang, Cheng Xu, Yingui Cao, Shengsheng Liu, Rui L. Reis, Subhas C. Kundu, Xiao Yang, Bo Xiao, Lian Duan
Summary: Pluronic F127 modified silk fibroin film with different types of antibacterial agents could accelerate wound recovery by promoting fibroblast adhesion, eradicating bacteria, and facilitating angiogenesis and re-epithelialization.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Article
Materials Science, Biomaterials
Yinsheng Liu, Mingyue Wang, Yinfei Hui, Lei Sun, Yanrui Hao, Henlong Ren, Hao Guo, Wu Yang
Summary: In this study, a rare-earth hybrid luminescent material was developed for the detection of a biomarker for anthrax. The material showed excellent selectivity and high sensitivity, allowing for the determination of the biomarker in saliva and urine. Additionally, a convenient point-of-care testing method using fluorescent test paper and a smartphone was established for the initial diagnosis of anthrax.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)
Review
Materials Science, Biomaterials
Wenshuai Yang, Jingsi Chen, Ziqian Zhao, Meng Wu, Lu Gong, Yimei Sun, Charley Huang, Bin Yan, Hongbo Zeng
Summary: Injectable hydrogels with shear-thinning and/or in situ formation properties offer distinct advantages in bioengineering applications, as they can be directly delivered to target sites, possess self-healing abilities, and simplify the implantation process.
JOURNAL OF MATERIALS CHEMISTRY B
(2024)