Article
Multidisciplinary Sciences
Cheng-Hai Zhang, Yao Gao, Han-Hwa Hung, Zhu Zhuo, Alan J. Grodzinsky, Andrew B. Lassar
Summary: Creb5 transcription factor regulates joint formation and the development of articular cartilage by blocking Wnt5a expression.
NATURE COMMUNICATIONS
(2022)
Review
Biotechnology & Applied Microbiology
Kai Qiao, Qi Chen, Yiguo Cao, Jie Li, Gang Xu, Jiaqing Liu, Xiaolin Cui, Kang Tian, Weiguo Zhang
Summary: Cartilage lesions and degenerative joint diseases are two leading contributors to global disability, with current cell-based therapies facing limitations such as high tumorigenicity risk and low retention rate. Non-stem cell-based therapy, particularly extracellular vesicles (EVs), have gained popularity as they have been shown to achieve therapeutic effects similar to stem cells in cartilage tissue engineering. Further research on the therapeutic role of EVs in these conditions is warranted, given their potential to offer effective treatment options in the future.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Maria Figueres-Onate, Rebeca Sanchez-Gonzalez, Laura Lopez-Mascaraque
Summary: Understanding the strategies used to study cell lineages in the brain and their role in deciphering functional clonal relationships among neural cells; future perspectives will focus on studying cell heterogeneity and the origins of different cell lineages.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Orthopedics
Kezhou Wu, Kar Wey Yong, Maha Ead, Mark Sommerfeldt, Tamara D. Skene-Arnold, Lindsey Westover, Kajsa Duke, Leila Laouar, Janet A. W. Elliott, Nadr M. Jomha
Summary: This study aimed to investigate whether the repair effect of vitrified particulated cartilage would be equivalent to or better than that of fresh cartilage. The experimental results showed that both fresh and vitrified particulated cartilage had equivalent regeneration within cartilage defects when evaluated at 6 months after surgery.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Article
Biology
Fan Zhang, Yuanyuan Wang, Ying Zhao, Manqi Wang, Bin Zhou, Xianpeng Ge, Di Chen
Summary: NFATc1 is a crucial regulator of articular chondrocyte differentiation, expressed in the early stage of joint development. It negatively regulates the transcriptional activity of the COL2A1 gene in articular cartilage progenitors, determining the differentiation of articular chondrocytes. These findings highlight the important role of NFATc1 in articular cartilage development.
Article
Cell & Tissue Engineering
Chase A. Pagani, Amanda K. Huber, Charles Hwang, Simone Marini, Karthik Padmanabhan, Nicholas Livingston, Johanna Nunez, Yuxiao Sun, Nicole Edwards, Yu-Hao Cheng, Noelle Visser, Pauline Yu, Nicole Patel, Joseph A. Greenstein, Husain Rasheed, Reagan Nelson, Karen Kessel, Kaetlin Vasquez, Amy L. Strong, Geoffrey E. Hespe, Jane Y. Song, Deneen M. Wellik, Benjamin Levi
Summary: The research discovered a specific lineage of cells as ectopic bone progenitors in the zeugopod, providing a new perspective on fate specification and multipotency acquired after injury.
Review
Physiology
Wenzheng Zhang, Chao Gao, Akaki Tsilosani, Rohan Samarakoon, Robert Plews, Paul J. Higgins
Summary: This article summarizes the recent advances in utilizing the Cre-LoxP system for tracking stem/progenitor cells in kidney development and maturity, as well as the development and characterization of various Cre drivers.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)
Article
Engineering, Biomedical
Hyuna Kim, Anna Wirasaputra, Farnaz Mohammadi, Aritra Nath Kundu, Jennifer A. E. Esteves, Laura M. M. Heiser, Aaron S. S. Meyer, Shelly R. R. Peyton
Summary: Breast cancer is a major cause of cancer-related deaths worldwide, primarily due to metastasis and dormancy. Dormancy, a prolonged period between tumor resection and relapse, poses significant challenges in current therapies. To overcome this, a live cell lineage approach is used to track and distinguish dormant cells from proliferative and dying cells in various in vitro microenvironments. The study reveals that the proportion of long-term quiescent cells is regulated by both intrinsic and extrinsic factors, with the highest percentage found in 3D collagen gels. This approach is expected to benefit the study of dormant tumor cells for biologists and bioengineers in the dormancy community.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Cell Biology
Wenguang Liu, Meng Feng, Peng Xu
Summary: Growing evidence has shown the strong potential of cartilage-derived progenitor cells (CPCs) in proliferation, migration, and differentiation. While CPCs play a crucial role in cartilage development and repair in immature tissue, they are unable to fully repair cartilage defects during maturation and aging. Internal factors such as chronic inflammation and external factors like mechanical loading and nutritional imbalances can hamper the healing and regeneration of cartilage by CPCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Orthopedics
Heyong Yin, Kelei Mao, Yufu Huang, Ai Guo, Lin Shi
Summary: This study aimed to evaluate the potential use of stem cells derived from tendon tissue for cartilage repair. The results showed that tendon stem cells could successfully attach to and proliferate on decellularized cartilage matrix, indicating their potential as reparative cell sources for promoting cartilage repair.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Article
Chemistry, Medicinal
Jiaxiang Gu, Bin Wang, Tianliang Wang, Naichen Zhang, Hongjun Liu, Jianchao Gui, Yiming Lu
Summary: In this study, the effects of cartilage progenitor cells (CPCs), bone marrow mesenchymal stem cells (BMSCs), and chondrocytes (CCs) on cartilage repair were investigated. The results showed that CPCs had higher proliferation ability compared to CCs and BMSCs. Moreover, CPCs seeded in agarose gel exhibited significantly higher levels of collagen, glycosaminoglycan, DNA content, and chondrocyte-related gene expression. Integration strength in the CPCs seeded gel was also higher than the other two gels. These findings suggest that CPCs have advantages over CCs and BMSCs as seed cells in tissue engineering for cartilage repair.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Review
Engineering, Biomedical
Liangbin Zhou, Jietao Xu, Andrea Schwab, Wenxue Tong, Jiankun Xu, Lizhen Zheng, Ye Li, Zhuo Li, Shunxiang Xu, Ziyi Chen, Li Zou, Xin Zhao, Gerjo J. V. M. van Osch, Chunyi Wen, Ling Qin
Summary: As a highly specialized connective tissue, articular cartilage has limited self-repair capacity after traumatic injuries, resulting in significant socioeconomic burden. Current clinical therapies for cartilage defects often lead to mechanically inferior fibrocartilage and have limited cost-effectiveness and durability. Therefore, there is an urgent need for innovative approaches to create a proregenerative microenvironment and regenerate hyaline-like cartilage. Acellular regenerative biomaterials provide a promising solution by creating a favorable local environment for repair without the concerns of cell-based treatments. This review summarizes the current understanding of endogenous cartilage repair, discusses the challenges of using regenerative biomaterials for cartilage repair, and highlights recent advances in their design and application.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Marija Mazor, Eric Lespessailles, Thomas M. Best, Mazen Ali, Hechmi Toumi
Summary: Recent research has shown that cells isolated from osteoarthritic cartilage have the potential to form hyaline-like cartilage tissue. In this study, the researchers examined the marker expression and chondrogenetic potential of cells from mild, moderate, and severe osteoarthritic tissue. They found that only cells from moderate osteoarthritis were able to form hyaline cartilage-like tissue, while cells from severe and mild osteoarthritis did not. These findings have implications for understanding the repair capacity of articular cartilage in osteoarthritis and suggest that these progenitor cells may be a candidate for cartilage repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Geriatrics & Gerontology
Hongzhi Hu, Weijian Liu, Caixia Sun, Qiuyuan Wang, Wenbo Yang, ZhiCai Zhang, Zhidao Xia, Zengwu Shao, Baichuan Wang
Summary: Articular cartilage has limited self-repair capacity, but recruiting endogenous mesenchymal stem/progenitor cells to injured sites for regeneration shows promise as an alternative approach. Challenges in this strategy include inadequate recruitment of cells and unfavorable tissue microenvironment post-injury.
Editorial Material
Oncology
Eunmi Lee, Yibin Kang
Summary: The combination of single-cell lineage tracing and RNA sequencing has opened up unprecedented opportunities for prospectively exploring metastatic dynamics. In a pancreatic cancer model, Simeonov et al. developed the macsGESTALT lineage recording system, revealing that hybrid EMT states and S100 expression are associated with increased metastatic abilities.
