Article
Plant Sciences
Maryam Rahmati Ishka, Elizabeth Brown, Alexa Rosenberg, Shawn Romanowsky, James A. Davis, Won-Gyu Choi, Jeffrey F. Harper
Summary: In Arabidopsis, ER-localized ACA1, 2, and 7 are essential for plant growth and pollen fertility. Single-gene knockouts had weak or undetected phenotypes, while the triple KO of aca1/2/7 showed decreased pollen transmission efficiency and smaller rosettes with lesions. Expression of transgenes encoding ACA1, 2, or 7 rescued both vegetative and reproductive phenotypes, suggesting biochemical redundancy. Loss of these ER-localized pumps resulted in male fertility and growth deficiencies.
Article
Plant Sciences
Zhan Li, Jeffrey F. Harper, Chrystle Weigand, Jian Hua
Summary: Calmodulin-regulated Ca2+ pumps play a crucial role in maintaining basal cytosol Ca2+ levels, which are important for biotic and abiotic stress responses in Arabidopsis. The resting cytosol Ca2+ levels have an impact on plant survival under chilling and heat-stress conditions, and the regulation of these levels is critical for plant immune activation and environmental responses. Loss-of-function mutants of the calmodulin-regulated Ca2+ pumps exhibited elevated resting cytosol Ca2+ levels and increased susceptibility to chilling and heat stress, indicating the importance of these pumps in plant stress tolerance.
Article
Cell Biology
Samar Sayedyahossein, Louise Thines, David B. Sacks
Summary: The Hippo signaling pathway regulates various cellular processes, including cell proliferation, differentiation, migration, and mechanosensation. Calcium ion (Ca2+) has been found to influence core components of the Hippo pathway. However, the crosstalk between Ca2+ signaling and the Hippo pathway is not fully understood.
CELLULAR SIGNALLING
(2023)
Article
Chemistry, Applied
Huagen Xu, Pei Qiao, Jiali Pan, Ziyi Qin, Xia Li, Hock Eng Khoo, Xinhong Dong
Summary: This study aimed to investigate the changes in peel color, physicochemical characteristics, textural properties, and peel ultrastructure between CaCl2-treated and water-soaked passion fruit during short-term storage. The results showed that CaCl2 treatment effectively maintained the appearance and color of the fruit, inhibited peel browning, and improved fruit quality. The treatment also delayed the senescence of passion fruit and preserved the structural integrity of the fruit peel by stimulating the relative expression of the PeCaM gene.
Article
Biochemistry & Molecular Biology
Ronghui Ma, Na Tian, Jiashui Wang, Minlei Fan, Bin Wang, Pengyan Qu, Shiyao Xu, Yanbing Xu, Chunzhen Cheng, Peitao Lu
Summary: This study identified and classified the Ca2+-ATPase gene family in banana. The expression of banana Ca2+-ATPases is influenced by Ca2+ concentration, which may be related to root growth and Ca accumulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Xiaobo Cai, Xiazhen Yu, Jiawen Yang, Lin Lu, Ning Hua, Xin Duan, Peiwu Ye, Lei Ni, Linhua Jiang, Wei Yang, Tingbo Liang, Peilin Yu
Summary: In this study, it was found that the TRPM2 channel plays a crucial role in the development and progression of HCC. Inhibition or silencing of TRPM2, as well as inhibition of the downstream signaling pathway, significantly suppressed the proliferation of HCC cells. Furthermore, inhibition or depletion of TRPM2 also slowed down the growth of HCC xenografts in mice, indicating its potential as a therapeutic target for HCC.
HEPATOLOGY COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Zhenghao Chen, Satoshi Watanabe, Hironori Hashida, Michio Inoue, Yasukazu Daigaku, Masahide Kikkawa, Kenji Inaba
Summary: This study used nanobody/megabody technologies to determine the structures of human SPCA1a in the ATP and Ca2+/Mn2+-bound (E1-ATP) state and the metal-free phosphorylated (E2P) state, revealing the unique mechanisms of SPCA1a-mediated Ca2+/Mn2+ transport.
Article
Biochemistry & Molecular Biology
Michal Marcinkowski, Tomas Pilzys, Damian Garbicz, Jan Piwowarski, Kaja Przygonska, Maria Winiewska-Szajewska, Karolina Ferenc, Oleksandr Skorobogatov, Jaroslaw Poznanski, Elzbieta Grzesiuk
Summary: FTO is an N-6-methyladenosine demethylase which can remove methyl groups from nucleic acids, forming complexes with other proteins. Research indicates an interaction between FTO and calmodulin (CaM), influencing calcium-binding loops in CaM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Jinhong Wei, Wenting Guo, Ruiwu Wang, John Paul Estillore, Darrell Belke, Yong-Xiang Chen, Alexander Vallmitjana, Raul Benitez, Leif Hove-Madsen, S. R. Wayne Chen
Summary: The physiological significance of PKA phosphorylation of RyR2 in the heart is still poorly understood. Recent structural studies have shown that the PKA phosphorylation site S2030 in RyR2 is located within a pathway that is important for the termination of Ca2+ release. We investigated the impact of S2030 mutations on Ca2+ release termination in cells and generated a mouse model to study the role of S2030 in a physiological setting.
CIRCULATION RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Yen-Lin Chen, Zdravka Daneva, Maniselvan Kuppusamy, Matteo Ottolini, Thomas M. Baker, Eliska Klimentova, Soham A. Shah, Jennifer D. Sokolowski, Min S. Park, Swapnil K. Sonkusare
Summary: This study demonstrates the role of TRPV4(SMC) channels in blood pressure regulation, showing that their activity increases resting blood pressure in normal mice. The study also reveals the opposite effects of α1AR-TRPV4(SMC) signaling and intraluminal pressure-TRPV4(SMC)-BK channel signaling on blood pressure. In hypertension, constrictor α1AR-PKCα-TRPV4 signaling is upregulated, while dilator pressure-TRPV4-BK channel signaling is disrupted, leading to increased vasoconstriction and elevated blood pressure.
Article
Multidisciplinary Sciences
Ronja Schiemann, Annika Buhr, Eva Cordes, Stefan Walter, Jurgen J. Heinisch, Paola Ferrero, Hendrik Milting, Achim Paululat, Heiko Meyer
Summary: In this study, the researchers found that the endopeptidase Neprilysin 4 plays an essential role in regulating SERCA and maintaining Ca2+ homeostasis in cardiomyocytes, thus contributing to proper heart function.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Plant Sciences
Yunjing Wang, Qian Gong, Zhenhui Jin, Yule Liu, Yiguo Hong
Summary: A new study reveals the genetic link between calcium signaling and RNA interference (RNAi) in plants, showing that wound-triggered calcium flux serves as an initial messenger for priming RNAi for its role in plant antiviral defense. This discovery provides insights into plant development and response to various stresses.
TRENDS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Na Tang, Wen Tian, Guang-Yuan Ma, Xiong Xiao, Lei Zhou, Ze-Zhi Li, Xiao-Xiao Liu, Chong-Yao Li, Ke-Han Wu, Wenjuan Liu, Xue-Ying Wang, Yuan-Yuan Gao, Xin Yang, Jianzhao Qi, Ding Li, Yang Liu, Wen-Sheng Chen, Jinming Gao, Xiao-Qiang Li, Wei Cao
Summary: Deficiency of TRPC1 and TRPC6 channels ameliorates LPS-induced heart failure, prolongs survival, and suppresses cardiac inflammation and ER Ca2+ release.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Xiao-Hua Zhang, Cheng Ma, Lu Zhang, Min Su, Juan Wang, Sheng Zheng, Teng-Guo Zhang
Summary: This study investigates the enhancement of salt tolerance in cucumber by the exogenous strigolactone analog GR24, as well as the role of calcium ion (Ca2+) and hydrogen peroxide (H2O2) in this regulation. The results show that GR24 helps to maintain ion balance, increase antioxidant enzyme activity, reduce lipid peroxidation, and improve osmotic balance and ionic homeostasis in cucumber seedlings under salt stress. The effects of GR24 are dependent on the presence of H2O2 and Ca2+.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Xinxin Zhang, Juan Tian, Sai Li, Yuying Liu, Ting Feng, Yunyun Wang, Yuanjin Li, Xinxin Huang, Dahui Li
Summary: This study revealed the characteristics of the CAM/CML family in Ginkgo biloba, including their distribution in the genome, sequence diversity, and expression patterns. The results showed that Ginkgo CAMs/CMLs may have certain conservation and diversity in evolution and expression. Transgenic experiments and electrophoresis mobility shift assays demonstrated the role of the Gb_30819 gene in Ca2+ binding activity.