Article
Multidisciplinary Sciences
Yuexia Wang, Khurts Shilagardi, Trunee Hsu, Kamsi O. Odinammadu, Takamitsu Maruyama, Wei Wu, Chyuan-Sheng Lin, Christopher B. Damoci, Eric D. Spear, Ji-Yeon Shin, Wei Hsu, Susan Michaelis, Howard J. Worman
Summary: Prelamin A, a farnesylated precursor of lamin A, can cause progeria syndrome when accumulated as progerin. Mutations in ZMPSTE24, the processing enzyme of prelamin A, can lead to progeroid disorders. This study generated a mouse model with permanently farnesylated prelamin A and found that these mice have extended lifespan and exhibit skeletal and nuclear defects during physiological aging.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Magdalena Klimek-Chodacka, Miron Gieniec, Rafal Baranski
Summary: The study demonstrates an efficient, recombinant DNA-free, multiplex gene-editing method using gRNA:Cas9 ribonucleoprotein (RNP) complexes delivered directly to plant protoplasts. By forming three RNPs in vitro and delivering them to protoplasts, various editing effects were achieved with high efficiency, showcasing the potential of this method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Hyeong Jae Kim, Peter C. W. Lee, Jeong Hee Hong
Summary: With the discovery of the role of the nuclear envelope protein lamin in human genetic diseases, further diverse roles of lamins have been elucidated. The roles of lamins have been addressed in cellular homeostasis including gene regulation, cell cycle, cellular senescence, adipogenesis, bone remodeling as well as modulation of cancer biology. The modulatory roles of lamin-A/C in stem cell differentiation, skin, cardiac regulation, and oncology have also been elucidated.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Pathology
Ji-Yeon Shin, Howard J. Worman
Summary: Nuclear envelope is composed of various components and mutations in genes encoding these components can lead to laminopathies, a group of diseases that selectively affect different tissues or organs. The mechanisms underlying these diseases are still unclear and current hypotheses focus on the impact of nuclear envelope alterations on gene expression and cellular mechanics.
ANNUAL REVIEW OF PATHOLOGY-MECHANISMS OF DISEASE
(2022)
Article
Plant Sciences
Joseph F. McKenna, Hardeep K. Gumber, Zachary M. Turpin, Alexis M. Jalovec, Andre C. Kartick, Katja Graumann, Hank W. Bass
Summary: The study demonstrates that nucleoskeletal proteins NCH1, NCH2, and MKAKU41 in maize have characteristic properties of LINC-associated plant nucleoskeletal proteins, impacting the nuclear periphery structure and overall nuclear architecture.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sotirios D. Zervopoulos, Aristeidis E. Boukouris, Bruno Saleme, Alois Haromy, Saymon Tejay, Gopinath Sutendra, Evangelos D. Michelakis
Summary: The study reveals a noncanonical pathway for nuclear import of PDC and the role of mitochondria clustering around the nucleus in this process, providing new insights into mitochondria-to-nucleus communication and nuclear metabolism.
Review
Cell Biology
Eric C. Schirmer, Leena Latonen, Sylvain Tollis
Summary: Research suggests that changes in nuclear size are associated with tumor metastasis. Recent studies have found that reversing tumor type-dependent nuclear size changes is correlated with reduced cell migration and invasion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Rhiannon M. Sears, Kyle J. Roux
Summary: Mutations in LMNA and BANF1 genes can cause progeria, leading to accelerated aging. The proteins produced by these genes are involved in nuclear envelope rupture and repair processes. A-type lamins are recruited to the rupture sites in a BAF-dependent manner. Progeria-associated mutations inhibit the recruitment of A-type lamins to nuclear ruptures.
Article
Biochemistry & Molecular Biology
Yuri Y. Shevelyov
Summary: Heterochromatin is mainly localized at the nuclear periphery by interacting with the nuclear lamina and nuclear pore complexes. Recent studies have shown that these interactions play a crucial role in maintaining its peripheral localization, and there are differences in chromatin interactions with the nuclear envelope in cell populations and individual cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Athanasios A. Panagiotopoulos, Konstantina Kalyvianaki, Paraskevi K. Tsodoulou, Maria N. Darivianaki, Dimitris Dellis, George Notas, Vangelis Daskalakis, Panayiotis A. Theodoropoulos, Christos A. Panagiotidis, Elias Castanas, Marilena Kampa
Summary: In this study, the nuclear localization signals (NLS) for Importins 4 and 5 were identified and validated. The sequences LPPRS(G/P)P and KP(K/Y)LV were found to be recognition motifs for Importins 4 and 5 binding, respectively. These NLS signals are involved in the nuclear transport of important proteins.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Lihong He, Wei Shao, Jiang Li, Fei Deng, Hualin Wang, Zhihong Hu, Manli Wang
Summary: This study systematically analyzed the nuclear localization of 154 ORFs encoded by AcMNPV, with 25 viral proteins found to be localized in the nucleus in the absence of virus infection. Upon virus infection, 11 proteins originally located in the cytoplasm were completely translocated into the nucleus, suggesting their nuclear import is facilitated by other viral or host proteins. Further experiments identified specific proteins that were imported into the nucleus with the assistance of other nuclear localization proteins.
JOURNAL OF GENERAL VIROLOGY
(2021)
Editorial Material
Pharmacology & Pharmacy
Elizabeth R. Smith, Jing-Quan Wang, Dong-Hua Yang, Xiang Xi Xu
Summary: Taxanes are a group of successful drugs commonly used in chemotherapy to treat malignant tumors. However, drug resistance to taxanes is a common issue in subsequent treatments. In this review, the focus is on experimental findings and ideas related to the mechanism of taxane resistance involving the cancer nuclear envelope, aiming to provide new insights for overcoming taxane resistance.
DRUG RESISTANCE UPDATES
(2022)
Article
Virology
Luis Adrian De Jesus-Gonzalez, Selvin Noe Palacios-Rapalo, Jose Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Carlos Daniel Cordero-Rivera, Bulmaro Cisneros, Ana Lorena Gutierrez-Escolano, Rosa Maria del Angel
Summary: Recent evidence suggests that nuclear elements play a role in the replication of Flaviviruses, with certain viral proteins being imported into the nucleus. Furthermore, nuclear localization of these proteins has been observed in infected cells. The presence of ZIKV NS3 in the nucleus has been investigated, showing that NS3 is imported into the nucleus via the importin pathway and exported back to the cytoplasm.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemical Research Methods
Li-Chun Cheng, Xi Zhang, Kanishk Abhinav, Julie A. Nguyen, Sabyasachi Baboo, Salvador Martinez-Bartolome, Tess C. Branon, Alice Y. Ting, Esther Loose, John R. Yates, Larry Gerace
Summary: By using engineered biotin ligase TurboID and quantitative proteomics, this study explored the neighborhoods of Emerin and LBR in cultured mouse embryonic fibroblasts. The analysis revealed 232 high confidence proximity partners interacting selectively with Emerin and/or LBR, with 49 shared by both.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Virology
Nhan L. T. Nguyen, Wei Wu, Nelly Pante
Summary: This study investigates the role of the nucleoprotein (NP) localization signals in the nuclear import of viral ribonucleoprotein complexes (vRNPs) during influenza A virus infection. The study demonstrates that both NLS1 and NLS2 of the NP protein are crucial for successful infection, indicating their important role in the infection cycle of the virus.