Article
Critical Care Medicine
Anthony J. Fischer, Sachinkumar B. Singh, Mason M. LaMarche, Lucas J. Maakestad, Zoe E. Kienenberger, Tahuanty A. Pena, David A. Stoltz, Dominique H. Limoli
Summary: Staphylococcus aureus and Pseudomonas aeruginosa are both abundant in sputum cultures of cystic fibrosis patients. Contrary to common assumption, there is no rapid replacement of S. aureus by P. aeruginosa. Many CF patients have long-term coinfection with these organisms.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Article
Immunology
Wusheng Deng, Yanmei Lei, Xiujia Tang, Dingbin Li, Jinhua Liang, Jing Luo, Liuyuan Liu, Wenshu Zhang, Liumei Ye, Jinliang Kong, Ke Wang, Zhaoyan Chen
Summary: This study investigated whether DNase I can inhibit the early formation of biofilms in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models. The results showed that DNase I significantly inhibited early biofilm formation in a dose-dependent manner, without affecting bacterial growth. Therefore, DNase I has therapeutic potential against empyema biofilms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
Lalitha Biswas, Friedrich Goetz
Summary: Cystic fibrosis is a genetic disorder characterized by recurrent lung infections caused by Staphylococcus aureus and Pseudomonas aeruginosa. The interactions between these pathogens play a significant role in their survival, antibiotic resistance, and disease progression. Understanding these interactions is crucial for the treatment and prevention of cystic fibrosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Ines Reigada, Paola San-Martin-Galindo, Shella Gilbert-Girard, Jacopo Chiaro, Vincenzo Cerullo, Kirsi Savijoki, Tuula A. Nyman, Adyary Fallarero, Ilkka Miettinen
Summary: When two clinically relevant bacterial pathogens, Staphylococcus aureus and Pseudomonas aeruginosa, co-culture in a biofilm, P. aeruginosa shows higher motility and lower pigment production, while S. aureus exhibits increased persistence due to lower abundances of proteins related to cell wall biosynthesis and cell division. Both species also display higher antimicrobial tolerance when co-cultured. This study provides insights into the dynamics of both the surfaceome and exoproteome of the dual-species biofilms.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Eryn E. Bernardy, Vishnu Raghuram, Joanna B. Goldberg
Summary: The interaction between Staphylococcus aureus and Pseudomonas aeruginosa in the lungs of cystic fibrosis patients is key to understanding the severity of respiratory infections. The survival of S. aureus is significantly higher when coinfected with P. aeruginosa, suggesting that interactions between these two bacteria promote coexistence in the CF lung environment.
MICROBIOLOGY SPECTRUM
(2022)
Review
Biochemistry & Molecular Biology
Vincent Jean-Pierre, Agathe Boudet, Pauline Sorlin, Quentin Menetrey, Raphael Chiron, Jean-Philippe Lavigne, Helene Marchandin
Summary: Staphylococcus aureus is a major human pathogen that can survive and cause diseases in various clinical settings, including Cystic Fibrosis. In Cystic Fibrosis, S. aureus is the most commonly identified opportunistic pathogen, and its colonization/infection can become chronic in about one third of patients. S. aureus's ability to form biofilm is a crucial factor contributing to its persistence and protection in the lungs of CF patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Wing-Hin Lee, Ramin Rohanizadeh, Ching-Yee Loo
Summary: This study developed a novel bioactive bone substitute with improved anti-biofilm activity by functionalizing with curcumin, which effectively inhibits biofilm formation and controls viable planktonic cells. Curcumin was successfully incorporated onto HA and sustainably released over 14 days, showing stronger anti-biofilm effect against Staphylococcus aureus. The curcumin-loaded HA composite could be an alternative antimicrobial agent to control the risk of infections in postsurgical implants.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Microbiology
Hui Zhao, Lu Liu, Lingshuang Yang, Qihui Gu, Ying Li, Jumei Zhang, Shi Wu, Moutong Chen, Xinqiang Xie, Qingping Wu
Summary: The study identified a strain FJKB0103 isolated from the rhizosphere of Garcinia mangostana with strong anti-MRSA activity. Through genomic analysis, the researchers discovered that DAPG produced by the strain was the compound responsible for its activity. DAPG was found to be effective against MRSA strains and could inhibit biofilm formation.
Article
Chemistry, Multidisciplinary
Alessandra Fusco, Vittoria Savio, Debora Stelitano, Adone Baroni, Giovanna Donnarumma
Summary: The intestinal microbiota interacts with intestinal epithelial cells to protect the host from external environment. The anti-biofilm activity of HBD-2 and HBD-3 can inhibit biofilm formation of P. aeruginosa and S. aureus. Exploiting endogenous antimicrobial peptides as a new anti-biofilm therapy may be an interesting prospect for treating chronic and multi-drug resistant infections.
APPLIED SCIENCES-BASEL
(2021)
Article
Food Science & Technology
Yulu Li, Haoran Wang, Xiaoying Zheng, Zhuo Li, Muxue Wang, Kunyao Luo, Chunling Zhang, Xiaodong Xia, Yutang Wang, Chao Shi
Summary: This project explored the antibacterial mechanism of DDAB towards Staphylococcus aureus and Pseudomonas aeruginosa, as well as its removal effect on biofilms. The study found that DDAB has bacteriostatic and biofilm removal effects, and the effect of DDAB combined with SAEW on biofilms is more significant than that of using the two methods separately.
FOOD RESEARCH INTERNATIONAL
(2022)
Review
Biochemistry & Molecular Biology
Isaac Martin, Valerie Waters, Hartmut Grasemann
Summary: The treatment of lung infection in cystic fibrosis is limited by the biofilm growth of pathogenic organisms. Interventions targeting bacteria, extracellular matrix components, and signaling pathways offer potential new therapeutic strategies to overcome current limitations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Jiwasmika Baishya, Jake A. Everett, Walter J. Chazin, Kendra P. Rumbaugh, Catherine A. Wakeman
Summary: Calprotectin, a transition metal chelating protein, plays a role in nutritional immunity and has antimicrobial activity. It interacts with biofilms of Pseudomonas aeruginosa and Staphylococcus aureus, leading to compositional changes in the biofilm extracellular polymeric substance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Min Lu, Yongli Li, Mei X. Wu
Summary: Multidrug-resistant bacteria pose a significant threat and are depleting treatment options. This study explores the combination of blue-laser and thymol to eradicate these bacteria, successfully preventing systematic dissemination in mice. The strategy leverages the unique properties of bacteria to transform harmless thymol into a powerful sterilizer when activated by blue-laser excitation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Microbiology
Eu-Ri Jo, Jeonghyun Oh, Sung Il Cho
Summary: The study demonstrated that thymol has antibiofilm activity against MRSA and CRPA strains, effectively preventing biofilm formation and eradicating preformed biofilms, showing potential value for the treatment of persistent otorrhea in tympanostomy tubes.
Article
Multidisciplinary Sciences
Samuel L. Neff, Thomas H. Hampton, Charles Puerner, Liviu Cengher, Georgia Doing, Alexandra J. Lee, Katja Koeppen, Ambrose L. Cheung, Deborah A. Hogan, Robert A. Cramer, Bruce A. Stanton
Summary: Researchers have developed an application called CF-Seq, which allows users to filter studies and view differential gene expression analyses using a compendium of 128 studies and 1,322 samples.
Article
Multidisciplinary Sciences
Eszter Kovacs, Judit Sahin-Toth, Adrienn Tothpal, Mark van der Linden, Tamas Tirczka, Orsolya Dobay
Review
Dentistry, Oral Surgery & Medicine
Kasidid Ruksakiet, Lilla Hanak, Nelli Farkas, Peter Hegyi, Wuttapon Sadaeng, Laszlo Mark Czumbel, Thanyaporn Sang-ngoen, Andras Garami, Alexandra Miko, Gabor Varga, Zsolt Lohinai
JOURNAL OF ENDODONTICS
(2020)
Article
Multidisciplinary Sciences
Patrik Keringer, Nelli Farkas, Noemi Gede, Peter Hegyi, Zoltan Rumbus, Zsolt Lohinai, Margit Solymar, Kasidid Ruksakiet, Gabor Varga, Andras Garami
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Pongsiri Jaikumpun, Kasidid Ruksakiet, Balazs Stercz, Eva Pallinger, Martin Steward, Zsolt Lohinai, Orsolya Dobay, Akos Zsembery
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Judit Sahin-Toth, Eszter Kovacs, Adrienn Tothpal, Janos Juhasz, Barbara Forro, Krisztian Banyai, Kata Havril, Andrea Horvath, Agoston Ghidan, Orsolya Dobay
Summary: The study conducted in Hungary screened dogs and owners, finding a higher carriage rate of S. aureus in humans and more common presence of S. pseudintermedius in dogs. Although the isolates were generally susceptible to antibiotics, high tetracycline resistance was noted in S. pseudintermedius strains. Co-carried isolates shared similar resistance and virulence gene patterns, providing evidence for potential human-to-dog and dog-to-human transmission.
Article
Genetics & Heredity
James R. Knight, Eileen M. Dunne, E. Kim Mulholland, Sudipta Saha, Catherine Satzke, Adrienn Tothpal, Daniel M. Weinberger
Summary: The new software tool SeroCall can accurately identify and quantify different serotypes of Streptococcus pneumoniae in samples, even when multiple serotypes are present. This method achieved 100% accuracy for detecting the major serotype and up to 86% accuracy for detecting minor serotypes in mixed cultures.
MICROBIAL GENOMICS
(2021)
Article
Infectious Diseases
Janos Juhasz, Balazs Ligeti, Mario Gajdacs, Nora Makra, Eszter Ostorhazi, Ferenc Balazs Farkas, Balazs Stercz, Akos Toth, Judit Domokos, Sandor Pongor, Dora Szabo
Summary: Our study revealed that the composition of the microbial community plays a primary role in MDR colonization rate, while the antibiotic susceptibility of individual MDR strains has a lesser impact on this process. Distinct bacterial families have associated into microbial clusters, collecting taxonomically close species to produce survival benefits in the gut.
Article
Multidisciplinary Sciences
Balazs Stercz, Ferenc B. Farkas, Akos Toth, Mario Gajdacs, Judit Domokos, Viola Horvath, Eszter Ostorhazi, Nora Makra, Bela Kocsis, Janos Juhasz, Balazs Ligeti, Sandor Pongor, Dora Szabo
Summary: The study assessed the effects of antibiotics on the intestinal colonization of carbapenemase-producing Enterobacteriaceae (CPE) and found that ampicillin and ceftazidime increased the colonization rates of KP5825, while ciprofloxacin treatments led to significantly decreased colonization rates.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Kasidid Ruksakiet, Balazs Stercz, Gergo Toth, Pongsiri Jaikumpun, Ilona Grof, Roland Tengolics, Zsolt M. Lohinai, Peter Horvath, Maria A. Deli, Martin C. Steward, Orsolya Dobay, Akos Zsembery
Summary: The study reveals that bicarbonate modulates bacterial second messenger concentrations, inhibits biofilm formation, and could be used as a supportive treatment for CF and other respiratory diseases associated with chronic bacterial infections and viscous mucus production.
Article
Chemistry, Analytical
Eniko Farkas, Robert Tarr, Tamas Gerecsei, Andras Saftics, Kinga Dora Kovacs, Balazs Stercz, Judit Domokos, Beatrix Peter, Sandor Kurunczi, Inna Szekacs, Attila Bonyar, Anita Banyai, Peter Furjes, Szilvia Ruszkai-Szaniszlo, Mate Varga, Barnabas Szabo, Eszter Ostorhazi, Dora Szabo, Robert Horvath
Summary: There is a growing demand for bacteria repellent surfaces and antibody-based coatings in the field of biosensors. The optical waveguide lightmode spectroscopy (OWLS) technique offers a non-invasive and label-free method for characterizing protein and bacterial adsorption. In this study, an OWLS-based method was used to develop bacteria repellent surfaces and characterize different antibody-based coatings for bacterial assays. Nonspecific binding blocking agents were tested and immobilization methods for selected antibodies were applied. The performance of the biosensor was evaluated using enzyme-linked immunosorbent assay (ELISA) measurements. The best performance was achieved with a polyclonal antibody in combination with protein A-based immobilization and blocking of nonspecific binding.
Article
Biochemistry & Molecular Biology
Bassel Mansour, Adam Monyok, Mario Gajdacs, Balazs Stercz, Nora Makra, Kinga Penzes, Istvan Vadnay, Dora Szabo, Eszter Ostorhazi
Summary: The balance between the microbiome associated with bladder mucosa and human beta defensin (HBD) levels in urine is a dynamic and host-specific relationship. In bladder cancer patients, the levels of antibacterial and antitumor defensin (HBD1) decrease, while other defensins (HBD2 and HBD3) increase significantly. The composition of microbiome in tumor samples is also related to the levels of these defensins.
Article
Geriatrics & Gerontology
Dora Szabo, Eszter Ostorhazi, Balazs Stercz, Nora Makra, Kinga Penzes, Katalin Kristof, Istvan Antal, Janos M. Rethelyi, Reka I. Zsigmond, Ede Birtalan, Bela Merkely, Laszlo Tamas
Summary: The composition of nasopharyngeal microbiota plays a crucial role in individual susceptibility to SARS-CoV-2 infection. Corynebacteria, especially C. accolens strains, reduce the expression of ACE2, TMPRSS2, and cathepsin L receptors in the host, inhibit S1-ACE2 binding, and produce lipase. These findings suggest that C. accolens strains could be used as probiotics in the nasopharynx to decrease the individual susceptibility to SARS-CoV-2 infection.
Article
Veterinary Sciences
Katalin Reka Tarcsai, Igor Kapran, Mate Hidvegi, Balazs Stercz, Karoly Nagy, Jozsef Ongradi
MAGYAR ALLATORVOSOK LAPJA
(2020)
