Article
Engineering, Environmental
Charles P. Henriot, Helene Celle, Victor Klaba, Adrien Biguenet, Cecile Miege, Amandine Daval, Philippe Amiotte-Suchet, Jean-Charles Beugnot, Thomas Karbowiak, Xavier Bertrand
Summary: This study examined the effect of karst on antibiotic-resistant bacteria, specifically extended-spectrum beta-lactamases-producing Escherichia coli (ESBL-Ec), in a rural karst hydrosystem providing drinking water. The concentrations of ESBL-Ec were found to be spatially and temporally heterogeneous, with no filtration effect observed in the karst. The results suggest that karst poses a risk for the transmission of antibiotic-resistant bacteria.
Article
Environmental Sciences
Emily K. Rousham, Muhammad Asaduzzaman, T. I. M. Amin Uddin Mozmader, Mohammed Badrul Amin, Mahdia Rahman, Muhammed Iqbal Hossain, Md Rayhanul Islam, Zahid Hayat Mahmud, Leanne Unicomb, Mohammad Aminul Islam
Summary: The study revealed a high prevalence of ESBL-EC colonization in humans, but it was not significantly associated with poultry exposure. In these community settings, bidirectional transmission of antibiotic resistance between humans, poultry, and the environment is likely to occur, highlighting the importance of One Health mitigation strategies.
ENVIRONMENTAL HEALTH PERSPECTIVES
(2021)
Article
Infectious Diseases
Tristan Delory, Josselin Le Bel, Sylvie Lariven, Nathan Peiffer-Smadja, Francois-Xavier Lescure, Elisabeth Bouvet, Pauline Jeanmougin, Florence Tubach, Pierre-Yves Boelle
Summary: Online computerized decision support systems (CDSS) provide real-time information on the ecology and surveillance of E. coli resistance in community-acquired UTIs, assisting prescribers in selecting appropriate antimicrobial agents.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Environmental Sciences
Laurice Beatrice Raphaelle O. dela Pena, Mae Ashley G. Nacario, Nicole R. Bolo, Windell L. Rivera
Summary: This study detected multidrug-resistant Escherichia coli in samples collected around Laguna Lake in the Philippines and identified fecal and sewage sources as potential reservoirs of antibiotic-resistant bacteria. The results emphasize the importance of responsible antibiotic usage to mitigate the spread of antibiotic resistance.
Article
Public, Environmental & Occupational Health
Akinniyi Paul Akinduti, Oluwafunmilayo Ayodele, Babatunde Olanrewaju Motayo, Yemisi Dorcas Obafemi, Patrick Omoregie Isibor, Olubukola Wuraola Aboderin
Summary: The study evaluated the geospatial spread and antibiotic-resistant relatedness of Escherichia coli O157 in southwest Nigeria, which is an important virulent serotype causing severe complications. The results showed that human strains of stx-encoded Escherichia coli O157 were more prevalent compared to water and food animal strains, and exhibited high conjugative and transformative activity. The strains also displayed resistance to several common antibiotics. Analysis of the strains from different sources revealed similar biological characteristics and resistance patterns. The study highlights the importance of implementing One Health approach and population-target antimicrobial stewardship to mitigate the spread of antibiotic-resistant Escherichia coli O157 and reduce its impact on intestinal morbidity and mortality.
Article
Multidisciplinary Sciences
Daniel Schar, Cheng Zhao, Yu Wang, D. G. Joakim Larsson, Marius Gilbert, Thomas P. Van Boeckel
Summary: The study found concerning levels of resistance to medically important antimicrobials in aquatic food animals in Asia. The resistance rate in aquaculture remained stable between 2000 and 2018, while in fisheries it decreased. Resistance hotspots were identified along major river systems and coastal waters of China and India, with eastern China and India benefiting most from future surveillance efforts.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Gracie Hornsby, Temitope D. Ibitoye, Shivaramu Keelara, Angela Harris
Summary: The emergence of antimicrobial resistant (AMR) bacteria is a major public health threat of the 21st century. The World Health Organization recognizes the importance of environmental surveillance in understanding and combating the global rise of antimicrobial resistance. A modified IDEXX assay was used to enumerate cefotaxime resistant E. coli in environmental reservoirs and showed promising results, making it a potential method for global monitoring efforts.
ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS
(2023)
Article
Multidisciplinary Sciences
Claudia Cobo-Angel, Steven M. Roche, Stephen J. LeBlanc
Summary: This study aimed to investigate the factors influencing dairy cattle veterinarians' prescribing of antimicrobials and their attitudes towards reducing antimicrobial use in the dairy industry. The study found that veterinarians have to consider various factors and face conflicts when making prescribing decisions. Younger veterinarians showed higher awareness of antimicrobial resistance but also experienced more pressure to comply with farmers' wishes.
Article
Microbiology
Timothy J. Dallman, Saskia Neuert, Cristina Fernandez Turienzo, Michelle Berin, Emily Richardson, Pablo Fuentes-Utrilla, Nicholas Loman, Saheer Gharbia, Claire Jenkins, Ron H. Behrens, Gauri Godbole, Michael Brown
Summary: International travel is a risk factor for acquisition of resistant organisms, as shown by increased abundance and diversity of antibiotic resistance genes in fecal samples post-travel. Resistance genes for macrolides, third-generation cephalosporins, aminoglycosides, and sulfonamides were particularly increased. The study also found a significant association between diarrhea or antibiotic use and increased resistome abundance.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
Daniel Yu, Kanghee Ryu, Shuai Zhi, Simon J. G. Otto, Norman F. Neumann
Summary: Antibiotic resistance is a pressing public health concern driven by the overuse of antibiotics and environmental factors. The development of sanitation engineering has contributed to the spread of resistance and the evolution of resistance to water treatment. Pathogenic microbes may be developing resistance to both antibiotics and water treatment, which are important interventions for controlling infectious diseases in modern society.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Fisheries
Soon Bum Shin, Sung Rae Cho, Jong Soo Mok
Summary: The safety of seafood is a critical public health concern in Korea due to the high rate of raw seafood consumption. A study was conducted in 2018 to investigate the prevalence and antimicrobial resistance of fecal-associated bacteria in seawater samples and aquatic animals collected from aquaculture farms along the Korean coast. The results showed that Escherichia coli was the most prevalent and exhibited resistance to multiple antimicrobials.
AQUACULTURE RESEARCH
(2023)
Article
Cell Biology
Timothy Kudinha, Fanrong Kong
Summary: The prevalence of ST131, especially among ciprofloxacin resistant isolates, increased from fecal to clinical samples in women, men, and children. ST131 isolates showed higher virulence potential compared to non-ST131 isolates, particularly in urinary samples. There was a strong association between ST131 prevalence and fluoroquinolone resistance, with FQ resistant ST131 isolates harboring more virulence genes.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
An-Ni Zhang, Jeffry M. Gaston, Chengzhen L. Dai, Shijie Zhao, Mathilde Poyet, Mathieu Groussin, Xiaole Yin, Li-Guan Li, Mark C. M. van Loosdrecht, Edward Topp, Michael R. Gillings, William P. Hanage, James M. Tiedje, Katya Moniz, Eric J. Alm, Tong Zhang
Summary: Antibiotic resistance genes are common among bacteria, but not all pose high risks to human health. Researchers have developed an omics-based framework to rank these genes by risk, taking into account their enrichment in human associated environments, gene mobility, and host pathogenicity.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Saleh Afsharikhah, Reza Ghanbarpour, Parvin Mohseni, Nasrin Adib, Mahboube Bagheri, Maziar Jajarmi
Summary: This study aimed to assess the prevalence of common beta-lactam resistance genes and phenotypic resistance in urinary tract infections caused by Escherichia coli isolates. The results showed high prevalence of antibiotic resistance to beta-lactams and fluoroquinolones, suggesting the need for changes in drug resistance diagnosis and antibiotic therapy strategies.
Article
Infectious Diseases
Abdullahi B. Inuwa, Qaisar Mahmood, Jamshed Iqbal, Emilie Widemann, Sarfraz Shafiq, Muhammad Irshad, Usman Irshad, Akhtar Iqbal, Farhan Hafeez, Rashid Nazir
Summary: This study investigated the use of microalgae strains for municipal wastewater treatment and found significant improvements in the physicochemical qualities of the wastewater, with some genes being reduced beyond detectable levels within the first 4 days of treatment. Monocultures were more consistent in gene suppression compared to multi-species combinations. Water quality parameters such as TDS, TSS, and E. coli proved to be reliable proxies for ARGs mitigation.
Article
Infectious Diseases
Alexandra K. Heaney, Kathleen A. Alexander, Jeffrey Shaman
Article
Microbiology
Nicole A. Lerminiaux, Keith D. MacKenzie, Andrew D. S. Cameron
Article
Ecology
Kathleen Anne Alexander, Carol Anne Nichols
FRONTIERS IN ECOLOGY AND EVOLUTION
(2020)
Article
Biodiversity Conservation
Claire E. Sanderson, Kathleen A. Alexander
GLOBAL CHANGE BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Stephen Fitzgerald, Stefani C. Kary, Ebtihal Y. Alshabib, Keith D. MacKenzie, Daniel M. Stoebel, Tzu-Chiao Chao, Andrew D. S. Cameron
NUCLEIC ACIDS RESEARCH
(2020)
Article
Ecology
Kelton Verble, Eric M. Hallerman, Kathleen A. Alexander
Summary: The population genetic structure of banded mongooses in northern Botswana, infected with M. mungi, showed detectable differentiation and gene flow between troops in urban and natural landscapes. Individuals infected with M. mungi tended to stay within their natal groups, suggesting a link between dispersal behavior and infection. Urban landscapes may increase gene flow and dispersal behavior, potentially leading to higher risk of pathogen spread.
ECOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Joshua P. Ramsay, Tahlia R. Bastholm, Callum J. Verdonk, Dinah D. Tambalo, John T. Sullivan, Liam K. Harold, Beatrice A. Panganiban, Elena Colombi, Benjamin J. Perry, William Jowsey, Calum Morris, Michael F. Hynes, Charles S. Bond, Andrew D. S. Cameron, Christopher K. Yost, Clive W. Ronson
Summary: The horizontal transfer of ICEMlSym (R7A) can cause non-symbiotic Mesorhizobium spp. to become nitrogen-fixing legume symbionts. A subpopulation of Mesorhizobium japonicum R7A called R7A* is epigenetically primed for quorum sensing and horizontal transfer, maintained in laboratory culture. The epigenetic maintenance of the R7A* state requires ICEMlSym (R7A)-encoded copies of both qseC and qseC2, forming a stable epigenetic switch that controls qseM transcription and primes a subpopulation of R7A cells for quorum sensing and horizontal transfer.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Lawa Shaban, Anna S. Ershova, Fergal J. Hamrock, Ali Shaibah, Maha M. Sulimani, Mohammad R. Amin, Jennifer N. Russell, Rushiil Ravichandran, Kirsten Schaffer, Marta Martins, Andrew D. S. Cameron, Carsten Kroger
Summary: The draft genome sequence of Acinetobacter soli AS15, isolated from a rectal screen of a patient at St. Vincent's University Hospital (Dublin, Ireland) in 2018, consists of 3,589,002 bp and was assembled into 82 contigs.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Biochemistry & Molecular Biology
Mohammad R. Amin, Lisa Korchinski, Joshua K. Yoneda, Rishi Thakkar, Carla L. A. Sanson, Stephen F. Fitzgerald, Rod A. Kelln, Andrew D. S. Cameron
Summary: A point mutation in the putative cyclic AMP receptor protein (CRP) binding site was found to regulate the expression of inner membrane transporter DctA in Salmonella enterica and Escherichia coli. This mutation allowed the mutant dctA promoter to respond to CRP even in the presence of glucose, and resulted in higher dctA expression in the mutant compared to the wild type in the presence of alternative carbon sources that activate CRP.
CANADIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Supriya Bhat, Heather Maughan, Andrew D. S. Cameron, Christopher K. Yost
Summary: Delftia is a diverse genus of bacteria with ecological versatility and industrial relevance. Phylogenomic comparison of published genomes revealed distinct clades and highlighted the importance of industrially relevant functions in their ecological roles.
MICROBIAL GENOMICS
(2022)
Article
Biology
Kevin S. Kuchinski, Kara D. Loos, Danae M. Suchan, Jennifer N. Russell, Ashton N. Sies, Charles Kumakamba, Francisca Muyembe, Placide Mbala Kingebeni, Ipos Ngay Lukusa, Frida N'Kawa, Joseph Atibu Losoma, Maria Makuwa, Amethyst Gillis, Matthew LeBreton, James A. Ayukekbong, Nicole A. Lerminiaux, Corina Monagin, Damien O. Joly, Karen Saylors, Nathan D. Wolfe, Edward M. Rubin, Jean J. Muyembe Tamfum, Natalie A. Prystajecky, David J. McIver, Christian E. Lange, Andrew D. S. Cameron
Summary: This study evaluated the feasibility of using hybridization probe capture technique to recover more extensive genomic information from specimens. The results showed that probe capture achieved more genome sequence compared to standard amplicon sequencing protocols, and identified new coronaviruses. The study suggests that probe capture can be effectively used alongside other sequencing technologies for high-throughput discovery and surveillance of coronaviruses.
Article
Microbiology
Joseph P. P. Nowlan, Ashton N. N. Sies, Scott R. R. Britney, Andrew D. S. Cameron, Ahmed Siah, John S. S. Lumsden, Spencer Russell
Summary: Tenacibaculum is a genus of Gram-negative bacteria found worldwide. This study focused on Tenacibaculum genomes from Norway, Chile, and Canada, specifically their impacts on salmon aquaculture. The researchers used sequencing techniques to select and analyze isolates from Canadian salmon with mouthrot, discovering several known and unknown species of Tenacibaculum with potential virulence and antimicrobial resistance genes.
Article
Microbiology
Danae M. Suchan, Kara D. Loos, Keith D. MacKenzie, Sean D. Workman, Lori Johnson, Andrew D. S. Cameron, David C. Alexander
Summary: Ignavigranum ruoffiae is a rare human pathogen. Strain CPL 242382-20 was isolated from a breast cyst in Manitoba, Canada. Whole-genome sequencing was conducted using Oxford Nanopore Technologies MinION and Illumina MiSeq platforms, revealing a circular chromosome of 1,949,382 bp with a G+C content of 39.68% and 1,765 protein-coding genes.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Barbara A. Brown-Elliott, Richard J. Wallace, Nancy L. Wengenack, Sean D. Workman, Andrew D. S. Cameron, Georgie Bush, M. Dolores Hughes, Stephanie Melton, Bibiana Gonzalez-Ramirez, Eliana Rodriguez, Kavya Somayaji, Courtney Klapperich, Mary Viers, Ayooluwa J. Bolaji, Emma Rempel, David C. Alexander
Summary: Macrolides are commonly used to treat infections caused by nontuberculous mycobacteria (NTM). Recently, inducible macrolide resistance has been observed in Mycobacterium chelonae, a rapidly growing mycobacteria species, due to a novel plasmid-mediated erm gene. The presence of this gene has also been detected in other mycobacteria species, indicating the potential spread of plasmid-mediated macrolide resistance and its implications for treatment.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Eric M. Bottos, Ebtihal Y. AL-shabib, Dayton M. J. Shaw, Breanne M. McAmmond, Aditi Sharma, Danae M. Suchan, Andrew D. S. Cameron, Jonathan D. Van Hamme