4.6 Article

Effect of mono- and dichromatic light quality on growth rates and photosynthetic performance of Synechococcus sp PCC 7002

Journal

FRONTIERS IN MICROBIOLOGY
Volume 5, Issue -, Pages -

Publisher

FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2014.00488

Keywords

cyanobacteria; photosynthesis; chlorophyll; phycobiliprotein; turbidostat; fluorescence

Categories

Funding

  1. Genomic Science Program (GSP)
  2. Office of Biological and Environmental Research (BER), U.S. Department of Energy (DOE)
  3. Pacific Northwest National Laboratory (PNNL) Biofuels Scientific Focus Area (BSFA)
  4. Laboratory Research and Development Program
  5. Linus Pauling Distinguished Post-doctoral Fellowship program at PNNL
  6. National Science Foundation [MCB-1021725]
  7. Battelle Memorial Institute [DE-AC05-76RLO 1830]
  8. Direct For Biological Sciences
  9. Div Of Molecular and Cellular Bioscience [1021725] Funding Source: National Science Foundation

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Synechococcus sp. PCC 7002 was grown to steady state in optically thin turbidostat cultures under conditions for which light quantity and quality was systematically varied by modulating the output of narrow-band LEDs. Cells were provided photons absorbed primarily by chlorophyll (680 nm) or phycocyanin (630 nm) as the organism was subjected to four distinct mono- and dichromatic regimes. During cultivation with dichromatic light, growth rates were generally proportional to the total incident irradiance at values <275 mu mol photons m(-2) . s(-1) and were not affected by the ratio of 630:680 nm wavelengths. Notably, under monochromatic light conditions, cultures exhibited similar growth rates only when they were irradiated with 630 nm light; cultures irradiated with only 680 nm light grew at rates that were 60-70% of those under other light quality regimes at equivalent irradiances. The functionality of photosystem II and associated processes such as maximum rate of photosynthetic electron transport, rate of cyclic electron flow, and rate of dark respiration generally increased as a function of growth rate. Nonetheless, some of the photophysiological parameters measured here displayed distinct patterns with respect to growth rate of cultures adapted to a single wavelength including phycobiliprotein content, which increased under severely light-limited growth conditions. Additionally, the ratio of photosystem II to photosystem I increased similar to 40% over the range of growth rates, although cells grown with 680 nm light only had the highest ratios. These results suggest the presence of effective mechanisms which allow acclimation of Synechococcus sp. PCC 7002 acclimation to different irradiance conditions.

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