Article
Biochemistry & Molecular Biology
Wenlong Zhao, Huixia Zhu, Feng Wei, Donglai Zhou, Youguo Li, Xue-Xian Zhang
Summary: The introduction of exogenous mreB into Mesorhizobium huakuii resulted in enlarged cell size and slower growth, as well as the formation of ineffective nodules on Astragalus sinicus. The study revealed that MreB interacts with FtsZ(1) and FtsZ(2) in rhizobia, leading to the cessation of symbiosis at the late stage of bacteroid differentiation. This suggests that the loss of mreB in the common ancestor of Rhizobiales and subsequent acquisition of ftsZ(2) are critical evolutionary steps in the development of legume-rhizobial symbiosis.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Ginaini Grazielli Doin de Moura, Saida Mouffok, Nil Gaudu, Anne-Claire Cazale, Marine Milhes, Tabatha Bulach, Sophie Valiere, David Roche, Jean-Baptiste Ferdy, Catherine Masson-Boivin, Delphine Capela, Philippe Remigi
Summary: During the experimental evolution of a plant pathogenic bacterium into a legume symbiont, improved competitiveness for host entry was the main driver of fast adaptation, outweighing adaptation to within-host proliferation. Continuous accumulation of new mutations and sequential sweeps of mutations were observed, with multiple adaptive mutations co-occurring in the same cohort. Selective bottlenecks before within-host proliferation and after the hypermutagenesis phase were found to alter the relative influence of selective pressures during bacterial adaptation to multistep infection processes.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Plant Sciences
Haibo Huo, Le Zong, Yao Liu, Wenfeng Chen, Juan Chen, Gehong Wei
Summary: In this study, the researchers found that hmuS(pSym) of M. amorphae CCNWGS0123 plays a crucial role in rhizobial infection, nitrogen fixation, and nodule development in symbiosis with Robinia pseudoacacia. The protein HmuS(pSym) is involved in heme-binding and is strongly expressed in the nitrogen-fixation zone of mature nodules. The inactivation of hmuS(pSym) leads to the impairment of bacteroid survival and defense reaction in root nodules.
PLANT CELL AND ENVIRONMENT
(2022)
Review
Plant Sciences
Justin P. Hawkins, Ivan J. Oresnik
Summary: The interaction between bacteria and plants can be positive, negative, or neutral. The rhizobium-legume interaction is a well-studied positive interaction model. This review discusses how rhizobia tolerate stresses and how these stress tolerance mechanisms play a role in signal exchange between rhizobia and legumes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Bikash Raul, Oindrila Bhattacharjee, Amit Ghosh, Priya Upadhyay, Kunal Tembhare, Ajeet Singh, Tarannum Shaheen, Asim Kumar Ghosh, Ivone Torres-Jerez, Nick Krom, Josh Clevenger, Michael Udvardi, Brian E. Scheffler, Peggy Ozias-Akins, Ravi Datta Sharma, Kaustav Bandyopadhyay, Vineet Gaur, Shailesh Kumar, Senjuti Sinharoy
Summary: The study reveals dynamic changes in plant gene expression during peanut nodule development using comprehensive analysis and transcriptomics. Compared to other model legumes, peanut demonstrates diversification in oxygen-scavenging mechanisms and molecular mechanisms of terminal bacteroid differentiation.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Microbiology
Marta Ballesteros-Gutierrez, Marta Albareda, Coral Barbas, Angeles Lopez-Gonzalvez, Michael F. Dunn, Jose M. Palacios
Summary: Rhizobium leguminosarum bv. viciae (Rlv) UPM791 effectively nodulates pea and lentil, and a host-dependent protein called C189 was identified to have diaminobutyrate-2-oxoglutarate aminotransferase (DABA-AT) activity. The dat gene was found to be strongly induced in pea nodules, but not in lentil. Mutants defective in dat showed impaired symbiotic performance with pea plants, while no significant differences were observed in symbiosis with lentil. Comparative metabolomic analysis revealed differences in pea bacteroids, but not in lentil. The presence of L-homoserine and the absence of 2,4-diaminobutyrate (DABA) in pea nodules indicate that the Dat enzyme is involved in the adaptation mechanism of Rlv UPM791 to a homoserine-rich environment.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Christopher Carlson, Megan E. Frederickson
Summary: Mutualistic relationships can mask underlying conflicts of interest, and it is debated how often symbionts cheat their hosts and hosts control uncooperative symbionts. This antagonistic coevolution should result in selective sweeps or balancing selection in host and symbiont genomes. However, the genomic analyses suggest that there is little ongoing fitness conflict between legumes and rhizobia in this system.
Review
Agronomy
Sara Fahde, Said Boughribil, Badreddine Sijilmassi, Ahmed Amri
Summary: For many years, the scientific community focused on understanding how rhizobia promote the growth of legumes through nitrogen fixation. However, recent research has shown that rhizobia can also stimulate the growth of non-legume plants through various mechanisms such as hormone production, nutrient uptake improvement, pathogen control, and water regulation. This review aims to analyze the existing knowledge on how rhizobia promote the growth of non-legumes and gain new insights into their interactions.
Review
Biotechnology & Applied Microbiology
W. F. Chen, E. T. Wang, Z. J. Ji, J. J. Zhang
Summary: Symbiotic rhizobia convert dinitrogen gas into ammonia for the host plant. Specificity exists between rhizobia and leguminous species. Nodulation specificity and efficiency are mainly determined by soil conditions and host plants.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Review
Plant Sciences
Yanlin Ma, Rujin Chen
Summary: Nitrogen and phosphorus are essential mineral elements for plant growth and development, particularly in the symbiotic N fixation of legumes. Plants have evolved complex signaling networks to respond to phosphates and nitrates, optimizing symbiotic N fixation. Research in model organisms has provided insight into the signaling and transport processes of N and P during symbiosis.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Irene Jimenez-Guerrero, Carlos Medina, Jose Maria Vinardell, Francisco Javier Ollero, Francisco Javier Lopez-Baena
Summary: Rhizobia establish a symbiotic association with legumes, forming plant nodules where they fix atmospheric nitrogen into ammonia. The type 3 secretion system (T3SS) plays a crucial role in several rhizobium-legume symbioses by injecting effectors into host cells to alter host pathways or suppress host defense responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Plant Sciences
Shunxin Zhou, Chanjuan Zhang, Yi Huang, Haifeng Chen, Songli Yuan, Xinan Zhou
Summary: Delaying nodule senescence in legume crops can improve crop yield and reduce nitrogen fertilizer use, but effective measures are currently lacking. The review summarized the characteristics of nodule senescence and key genes involved, as well as factors affecting nodule senescence, providing a foundation for future research.
Review
Microbiology
Albin Teulet, Alicia Camuel, Xavier Perret, Eric Giraud
Summary: The type III secretion systems (T3SSs) used by rhizobia deliver effector proteins (T3Es) inside host cells to suppress plant immunity and promote infection for nitrogen fixation. The immune system of legume hosts and the cocktail of T3Es secreted by rhizobia determine the outcome of their symbiotic interaction. Rhizobial T3Es can either reduce plant immunity and promote infection or trigger defense responses if recognized by plant receptors. Some rhizobial T3Es can also bypass nodulation factors to induce nodule formation.
ANNUAL REVIEW OF MICROBIOLOGY
(2022)
Article
Plant Sciences
Sanhita Chakraborty, Oswaldo Valdes-Lopez, Christina Stonoha-Arther, Jean-Michel Ane
Summary: This article explores the transcriptional regulation mechanism of the symbiotic relationship between legume roots and rhizobia, highlighting the role of certain transcription factors in symbiosis and the response to the abiotic environment. The article also discusses the potential utility of modern methods in studying transcription factor-target interactions in plants.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nico Nouwen, Jean-Francois Arrighi, Djamel Gully, Eric Giraud
Summary: The ribBX gene in Bradyrhizobium ORS285 is not essential for riboflavin biosynthesis under free-living conditions, but may be needed to sustain riboflavin biosynthesis under certain symbiotic conditions.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Bettina Szerencses, Attila Gacser, Gabriella Endre, Ildiko Domonkos, Hilda Tiricz, Csaba Vagvolgyi, Janos Szolomajer, Dian H. O. Howan, Gabor K. Toth, Ilona Pfeiffer, Eva Kondorosi
Summary: This study demonstrates the anticandidal activity of NCR335 and NCR169 peptide derivatives against five Candida species, showing the ability to inhibit biofilm formation and yeast-hypha transformation. Combined treatment with fluconazole revealed synergistic interactions and reduced minimal inhibitory concentrations of C. auris. Shortening NCR peptides can enhance and broaden their anticandidal activity and therapeutic potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Wendy A. Stirk, Peter Balint, Gergely Maroti, Zoltan Varga, Zsuzsanna Lantos, Johannes van Staden, Vince Ordog
Summary: The study examined the growth of three Chlorellaceae strains in outdoor culture, finding that Micractinium sp. was the most suitable for outdoor cultivation with the highest biomass production and least affected by environmental conditions. Mixed culture of Chlorellaceae showed good biomass production performance but lower lipid content, with antioxidant and plant-stimulating activities varying with strain and culture age.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Microbiology
Quentin Nicoud, Quentin Barriere, Nicolas Busset, Sara Dendene, Dmitrii Travin, Mickael Bourge, Romain Le Bars, Claire Boulogne, Marie Lecroel, Sandor Jenei, Atilla Kereszt, Eva Kondorosi, Emanuele G. Biondi, Tatiana Timchenko, Benoit Alunni, Peter Mergaert
Summary: Various functions in Sinorhizobium meliloti are involved in protecting endosymbionts, including peptide transporters, bacterial envelope structures, and stress response regulators.
Article
Cell Biology
Vaishali Rani, Gergely Maroti
Summary: The study found that Chlamydomonas sp. MACC-216 and Chlorella sp. MACC-360 were able to remove nitrate from water bodies, with decreasing total pigment content and increasing lipid content as nitrate concentration increased. Both microalgae demonstrated good growth and nitrate removal capacity in synthetic wastewater.
Article
Plant Sciences
Margaret Mukami Gitau, Attila Farkas, Benedikta Balla, Vince Ordog, Zoltan Futo, Gergely Maroti
Summary: This study investigated the plant-growth-promoting effects of three selected strains of eukaryotic green microalgae on Medicago truncatula plants. The results showed that the application of live algae cells significantly increased plant growth parameters, with Chlorella species demonstrating the most pronounced effects.
Article
Energy & Fuels
Mark Szuhaj, Roland Wirth, Zoltan Bagi, Gergely Maroti, Gabor Rakhely, Kornel L. Kovacs
Summary: The study focused on the performance of a mixed microbial community in lab-scale power-to-methane reactors at 55°C, revealing the community's response to different levels of H2 supply. Low H2 triggered immediate CH4 evolution, while high H2 doses inhibited biomethane formation. However, biomethane production continued steadily with the daily delivery of stoichiometric H2/CO2.
Article
Biochemistry & Molecular Biology
Balint Barath, David K. Jasz, Tamara Horvath, Bence Barath, Gergely Maroti, Gerda Strifler, Gabriella Varga, Lilla Sandor, Domonkos Perenyi, Szabolcs Tallosy, Tibor Donka, Peter Javor, Mihaly Boros, Petra Hartmann
Summary: This study found that antibiotics such as ceftriaxone and rifaximin, despite being clinically effective, can cause mitochondrial dysfunction and have negative effects on the intestinal mucosa. They also alter the composition and diversity of the gut microbiome, although they do not increase the abundance of toxin-producing species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Zane Vitenberga-Verza, Mara Pilmane, Ksenija Serstnova, Ivars Melderis, Lukasz Gontar, Maksymilian Kochanski, Andzelika Drutowska, Gergely Maroti, Beatriz Prieto-Simon
Summary: This study aimed to investigate the inflammatory and regulatory cytokines in the milk of dairy cows with subclinical and clinical mastitis. The results showed that IL-1 alpha, IL-4, and IL-17A drive the inflammatory responses in mastitis cases. Furthermore, the host defense response in mastitis is characterized by the continuation or resolution of the initial inflammation.
Article
Green & Sustainable Science & Technology
Margaret Mukami Gitau, Attila Farkas, Vince Ordog, Gergely Maroti
Summary: This study evaluated the biostimulant effects of two microalgae strains on tomato plants and found that they could promote plant growth and fruit development. The study also revealed strain-specific actions of the two algae strains on tomato.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Vaishali Rani, Gergely Maroti
Summary: In this study, Chlamydomonas sp. MACC-216 was used to investigate the removal of nitrate in TAP medium under different light conditions. It was found that both light color and intensity affected the efficiency of nitrate removal. Specifically, the blue + red light combination showed the highest nitrate removal efficiency and nitrate reductase activity in synthetic wastewater. These results suggest that the blue + red light combination, together with high light intensity, is a promising light condition for nitrate removal.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ecology
Roland Wirth, Zoltan Bagi, Prateek Shetty, Mark Szuhaj, Teur Teur Sally Cheung, Kornel L. Kovacs, Gergely Maroti
Summary: Multi-omics analysis was used to study inter-kingdom interactions in biogas-producing microbial communities. The microbiomes of three biogas digesters were analysed using a metagenomics framework, revealing the relationship between methanogenic communities and their bacterial partners. The study identified high-quality metagenome-assembled genomes and found that the abundance of microorganisms was independent of their activity. The most active metabolic pathway was found to be methanogenesis.
Article
Biotechnology & Applied Microbiology
Bettina Hupp, Gabriella Huszar, Attila Farkas, Gergely Maroti
Summary: Biohydrogen production from wastewater using eukaryotic green algae can be enhanced by selecting appropriate bacterial partners and cultivation conditions. The study found that specific Bacillus bacterial partners, especially Bacillus amyloliquefaciens, Bacillus mycoides, and Bacillus cereus, promoted algal biomass yield and hydrogen production. Co-culture combinations of Chlorella sp. MACC-360 with Bacillus amyloliquefaciens showed the highest algal hydrogen yields. The formation of co-culture-specific exopolysaccharides and tight physical interactions between the algal and bacterial partners were observed.
FERMENTATION-BASEL
(2023)
Article
Plant Sciences
Senlei Zhang, Ting Wang, Rui M. Lima, Aladar Pettko-Szandtner, Attila Kereszt, J. Allan Downie, Eva Kondorosi
Summary: Symbiotic nitrogen fixation by Rhizobium bacteria in legume root nodules alleviates the need for nitrogen fertilizers. Terminal differentiation of bacteroids is controlled by nodule-specific cysteine-rich (NCR) peptides, which are absent in legumes like soybean. The expression of NCR genes does not require NCR-specific transcription factors, but two AHL transcription factors found in Medicago truncatula and soybean nodules induce NCR gene expression. Mutation of NCR169 arrests bacteroid development, while the absence of MtAHL1 or MtAHL2 completely blocks bacteroid differentiation. These findings suggest the possibility of increasing nitrogen fixation efficiency in legumes lacking NCRs.
Article
Chemistry, Multidisciplinary
Gyula Kajner, Adam Belteki, Martin Cseh, Zsolt Geretovszky, Tibor Ajtai, Lilla Barna, Maria A. Deli, Bernadett Pap, Gergely Maroti, Gabor Galbacs
Summary: In this study, a completely 3D-printed polymer sample introduction system was developed for single particle ICP-MS analysis. The new system showed significant improvements in particle detection efficiency, signal-to-noise ratio, size detection limit, and the upper limit of transportable particle diameters.
Article
Biotechnology & Applied Microbiology
Bettina Hupp, Bernadett Pap, Attila Farkas, Gergely Maroti
Summary: Eukaryotic algae, particularly unicellular green microalgae, have the potential for biohydrogen production. This study focuses on the engineering of stable synthetic communities consisting of green algae and starch-degrading bacteria for continuous photoheterotrophic biohydrogen production. The results show that the engineered pairwise algal-bacterial associations can significantly increase biomass and biohydrogen yield compared to axenic conditions.
FERMENTATION-BASEL
(2022)