Article
Multidisciplinary Sciences
Miriam Zago, Lucia Massimiliano, Barbara Bonvini, Giuseppe Penna, Giorgio Giraffa, Maria Rescigno
Summary: This study investigated the functional characteristics of four strains of L. helveticus isolated from Italian hard cheeses, showing their immunomodulatory and antioxidant activities, and positive effects on the immune system.
Article
Food Science & Technology
Anna V. Begunova, Olga S. Savinova, Olga A. Glazunova, Konstantin V. Moiseenko, Irina V. Rozhkova, Tatyana V. Fedorova
Summary: Bioactive peptides derived from milk proteins have positive effects on various physiological systems and are considered promising ingredients for functional food. These peptides are usually inactive within parent proteins and can be released during milk fermentation by specific proteolytic systems of different Lactobacillus spp.
Article
Agriculture, Dairy & Animal Science
Lina Zhao, Qinggang Xie, Fengyi Shi, Shengnan Liang, Qingxue Chen, Smith Etareri Evivie, Ji Qiu, Bailiang Li, Guicheng Huo
Summary: This study aimed to reduce cow milk protein allergenicity by fermenting skim milk protein with Lactobacillus helveticus and Lactobacillus plantarum strains. The fermentation process inhibited antigenicity of specific milk proteins and cleaved major allergic epitopes, demonstrating a potential method for reducing milk sensitization.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Lina Zhao, Fengyi Shi, Qinggang Xie, Yifan Zhang, Smith Etareri Evivie, Xuetong Li, Shengnan Liang, Qingxue Chen, Bowen Xin, Bailiang Li, Guicheng Huo
Summary: This study aimed to evaluate the therapeutic potential of co-fermented milk protein in cow milk protein allergy management. It found that different fermentation methods can significantly alter the conformational structures of the cow milk protein and reduce its potential allergenicity by regulating the immune response balance.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Chemistry, Applied
Naoki Shirotani, Anni Bygvraa Hougaard, Rene Lametsch, Mikael Agerlin Petersen, Fergal P. Rattray, Richard Ipsen
Summary: The study found that during the fermentation of soy protein isolates, the Lactobacillus helveticus strain LH88 can specifically degrade proteins, generating free amino acids and volatile compounds. However, most proteins remained intact after seven days of fermentation, suggesting that further modifications to the fermentation conditions are needed to promote the production of flavor compounds.
Article
Food Science & Technology
Dan Hu, Jinyong Wu, Long Jin, Lixia Yuan, Jun Li, Xiangsong Chen, Jianming Yao
Summary: This study evaluated the fermentation characteristics and properties of soybean milk fermented by four Pediococcus pentosaceus strains. The results showed that these strains exhibited antibacterial activity, with survival rates above 90% in simulated gastrointestinal environments, satisfying probiotic requirements. Among the strains, QK-1 showed the weakest acidification of fermented soybean milk, and all strains maintained stable bacterial counts after 28 days of storage, with enhanced antioxidant ability post-fermentation.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Mingzhe Wang, Wanyi Zhou, Ying Yang, Jianrong Xing, Xiaodan Xu, Yuqing Lin
Summary: The EPS derived from Lactobacillus pentosus strain YY-112 has beneficial effects on the intestinal microbiota and short-chain fatty acid production, making it a potential probiotic strain.
Article
Food Science & Technology
Kobika Chelladhurai, Mutamed Ayyash, Mark S. Turner, Afaf Kamal-Eldin
Summary: This article reviews the key features of L. helveticus in the dairy industry, including technological advantages, health effects, current applications, and future trends. L. helveticus produces active peptides through its strong proteolytic activity, which contribute to the flavor and health benefits of products. The consumption of L. helveticus-fermented dairy products has been shown to provide bioactive compounds with antimicrobial, antioxidant, antihypertensive, anticarcinogenic, gut-wellness, psychobiotic, and immunostimulatory effects.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Food Science & Technology
Wenping Lei, Jie Luo, Ku Wu, Qi Chen, Lulu Hao, Xingrong Zhou, Xianyue Wang, Chengguo Liu, Hui Zhou
Summary: Dendrobium candidum extract (DCE) has a positive impact on the fermentation of milk using probiotics, increasing viable bacteria count and improving the growth and acid production ability of the strain. Additionally, DCE enhances the ability to scavenge free radicals and survival rates in simulated gastrointestinal fluids.
Article
Biochemistry & Molecular Biology
Bogdan Goranov, Desislava Teneva, Rositsa Denkova-Kostova, Vesela Shopska, Nadia Oulahal, Zapryana Denkova, Georgi Kostov, Pascal Degraeve, Rafael Pagan
Summary: The study found that chocolate mousse can serve as a good medium for delivering probiotic bacteria and inhibiting the growth of foodborne pathogens.
Article
Food Science & Technology
Shuman Gao, Yang Jiang, Xinyi Zhang, Shumao Cui, Xiaoming Liu, Jianxin Zhao, Hao Zhang, Wei Chen
Summary: This study investigated the proteolysis patterns of different Lactobacillus helveticus strains with different cell envelope proteinase (CEP) genes during milk fermentation using peptidomics. The results showed that variations in peptide patterns of casein fractions were correlated with CEP genotypes, and the bioactive peptides derived from the common hydrolysis region exhibited antihypertensive effects.
Article
Food Science & Technology
Katarzyna Petka, Pawel Sroka, Tomasz Tarko, Aleksandra Duda-Chodak
Summary: Under specific conditions, Lactobacillus acidophilus LA-5 has the ability to degrade acrylamide, but this capability disappears under certain conditions. Additionally, the presence of acrylamide in milk can modulate the bacteria metabolism.
Article
Microbiology
Haripriya Gupta, Sung Hun Kim, Seul Ki Kim, Sang Hak Han, Hak Cheol Kwon, Ki Tae Suk
Summary: This study investigated the effects of probiotic treatment on alcoholic hepatitis patients by restoring gut microbial balance. The results showed that probiotics improved liver function, decreased lipopolysaccharide levels, and modulated the composition of gut microbiota.
Article
Microbiology
Hyeonji Kim, Kippeum Lee, Ju-Yeon Kim, Jae-Jung Shim, Junghyun Lim, Joo-Yun Kim, Jung-Lyoul Lee
Summary: Lactiplantibacillus plantarum LP158, Lactobacillus helveticus HY7804, and Lacticaseibacillus paracasei LPC226 isolated from raw milk have been shown to alleviate non-alcoholic fatty acid disease (NAFLD). These strains inhibit fatty acid accumulation, modulate gene expression related to lipogenesis and beta-oxidation, and improve blood biochemistry markers and liver steatosis in a mouse model of NAFLD. HY7804 exhibits the strongest effects both in vitro and in vivo, suggesting its potential as a supplement for NAFLD treatment.
Review
Agriculture, Dairy & Animal Science
Marta Michalak, Konrad Wojnarowski, Paulina Cholewinska, Natalia Szeligowska, Marcel Bawej, Jakub Pacon
Summary: The increasing production of ruminants has led to a focus on feed additives' impact on the microbiome for improved performance and reduced methane emissions. Research is shifting towards finding safe alternatives to antibiotics as ruminant populations grow.
Article
Microbiology
Katarina Novovic, Milka Malesevic, Lazar Gardijan, Milan Kojic, Branko Jovcic
Summary: The rapid and appropriate response of Pseudomonas spp. to environmental fluctuations is facilitated by various signal transduction regulatory systems. In this study, a novel activator of the intI1 transcription, sensor kinase RclS, was discovered in Pseudomonas putida WCS358. The regulatory mechanism is limited to the stationary growth phase and appears to be indirect, possibly through the regulation of rpoS expression. The RclSAR regulatory system seems to be part of a complex intI1 regulatory network that includes major stress response regulons.
RESEARCH IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lazar Gardijan, Marija Miljkovic, Mina Obradovic, Branka Borovic, Goran Vukotic, Goran Jovanovic, Milan Kojic
Summary: The aim of this study is to construct an improved pMAL expression vector for the efficient purification of small native peptides. By introducing a His(6) tag and an enterokinase cleavage site, the purification strategy was optimized, and successfully applied in the purification of active native small peptides.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Dusan Usjak, Katarina Novovic, Brankica Filipic, Milan Kojic, Nenad Filipovic, Magdalena M. Stevanovic, Marina T. Milenkovic
Summary: This study aimed to investigate the synergistic activity of colistin and selenium nanoparticles against pandrug-resistant Ac. baumannii. The results showed an exceptionally strong synergistic activity between colistin and SeNPs. This combination could be a new option to fight pandrug-resistant Ac. baumannii.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Brankica Filipic, Milka Malesevic, Zorica Vasiljevic, Katarina Novovic, Milan Kojic, Branko Jovcic
Summary: This study found that 33 out of 98 Achromobacter spp. isolates from pediatric patients were resistant to SXT, and among these, 10 resistant isolates were found to carry the intI1 gene. Genome sequencing revealed a deletion of 86 amino acids in the IntI1S protein compared to canonical IntI1 protein. The results suggest that IntI1S isolates are derived from clinical settings and that cystic fibrosis patients may serve as potential reservoirs for healthcare-associated infections in non-cystic fibrosis patients.
FOLIA MICROBIOLOGICA
(2023)
Article
Plant Sciences
Aleksandra Mesaros, Marija Nedeljkovic, Dario Danojevic, Sladjana Medic-Pap, Slavisa Stankovic, Svetlana Radovic, Jelena Lozo
Summary: With changing environmental conditions, food production is becoming increasingly challenging. One solution to this problem is the production of cultivars resistant to various abiotic and/or biotic stresses. In this study, the response of antioxidant system in two different bell pepper genotypes to Xanthomonas euvesicatoria was analyzed. The susceptible genotype showed a higher activity of antioxidant enzymes and concentration of ascorbic acid, while the tolerant genotype adapted through regulation of chlorophyll and carotenoid concentration. Obtaining a tolerant genotype through crossbreeding can provide tolerance to both biotic and abiotic stressors.
PLANT GROWTH REGULATION
(2023)
Article
Ecology
Milka Malesevic, Nemanja Stanisavljevic, Danka Matijasevic, Jovana Curcic, Vukasin Tasic, Srdjan Tasic, Milan Kojic
Summary: This study conducted a comprehensive analysis of microbial diversity in the Vranjska Banja hot spring using culture-independent and culture-dependent methods. Novel taxa were discovered, and their genomic characteristics were analyzed. These microbes have the potential to produce thermostable enzymes and antimicrobial molecules, which can be of great importance for various industrial, agricultural, and biotechnological applications.
Article
Virology
Mina Obradovic, Milka Malesevic, Mariagrazia Di Luca, Dusan Kekic, Ina Gajic, Olivia McAuliffe, Horst Neve, Nemanja Stanisavljevic, Goran Vukotic, Milan Kojic
Summary: Klebsiella pneumoniae is a global health threat and phages LASTA and SJM3 show potential in combating pandrug-resistant K. pneumoniae infections. The two phages have narrow host ranges and long latent periods, but are not lysogenic. Genome analysis reveals they belong to the new genus Lastavirus. In tests, both phages demonstrate significant bacterial reduction, with SJM3 performing slightly better.
Article
Microbiology
Katarina Novovic, Snezana Kuzmanovic Nedeljkovic, Mirjana Poledica, Gordana Nikolic, Bojana Grujic, Branko Jovcic, Milan Kojic, Brankica Filipic
Summary: Since the declaration of the COVID-19 pandemic, the disease has overwhelmed the health system, leading to an increase in ICU admissions. This study examines the antibiotic resistance and virulence potential of A. baumannii isolates recovered from COVID-19 patients requiring mechanical ventilation. The results reveal the presence of multidrug-resistant A. baumannii strains with varying levels of antibiotic resistance and virulence potential.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Tamara Krstic Tomic, Iva Atanaskovic, Ivan Nikolic, Natasa Jokovic, Tatjana Stevic, Slavisa Stankovic, Tanja Beric, Jelena Lozo
Summary: This study aimed to investigate whether 16S rRNA gene metabarcoding has predictive power in identifying the majority of known bacteria with plant-beneficial traits in the sugar beet microbiome. Through isolation and testing of bacterial isolates, it was found that culture-dependent analysis is necessary for identifying bacteria with beneficial traits, and plant-based media is effective for high-yield isolation of such bacteria.
Article
Biochemistry & Molecular Biology
Marinela Sokarda Slavic, Milan Kojic, Aleksandra Margetic, Nemanja Stanisavljevic, Lazar Gardijan, Natasa Bozic, Zoran Vujcic
Summary: Amylase from thermophilic bacterial strain Anoxybacillus vranjensis ST4 was cloned, expressed, and purified successfully. The enzyme showed maximum activity at 60-80°C and was active and stable over a wide pH range (4.0-9.0). It efficiently hydrolyzed starch granules, releasing maltose, maltotriose, and maltopentaose as the main products. These findings indicate that this highly stable and versatile enzyme has great potential for industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Marinela Sokarda Slavic, Milan Kojic, Aleksandra Margetic, Marina Ristovic, Marija Pavlovic, Stefan Nikolic, Zoran Vujcic
Summary: The study focused on the impact of the enzyme endo-beta-1,3-1,4-glucanase from Bacillus subtilis 168 on improving the nutritional and bioactive properties of barley beta-glucan. A new procedure for isolating pure beta-glucan from barley flour was developed, enabling it to be free from starch contamination. The cloned enzyme showed high efficiency in degrading beta-glucan and increasing its antioxidant capacities, suggesting potential applications in functional foods.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Entomology
Nemanja Stanisavljevic, Milka Malesevic, Sladan Rasic, Goran Vukotic, Lazar Gardijan, Mina Obradovic, Milan Kojic
Summary: In this study, honey bees were fed with a diet supplemented with spores of Brevibacillus laterosporus BGSP11. The short-term effects on their micro- and mycobiota were analyzed using Illumina MiSeq sequencing. The results show that this treatment does not harm the bacterial microbiome of the bees, but slightly affects the composition of core microbiota. The treatment leads to an increase in beneficial bacteria species and a reduction in potential pathogens. However, it has a more profound effect on the mycobiota, reducing phytopathogenic fungi that can affect the quality of honey.
JOURNAL OF APICULTURAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Jelena Lozo, Nemanja Ristovic, Goran Kungulovski, Zivko Jovanovic, Tamara Rakic, Slavisa Stankovic, Svetlana Radovic
Summary: Rhizosphere microbial communities play a key role in plant health and productivity. The rhizobacteria Pseudomonas putida P2 and Bacillus cereus P5 were effective in promoting plant growth and alleviating oxidative stress in the Belija wheat cultivar under drought conditions. Next Generation Sequencing revealed extremophilic bacteria such as Vicinamibacter silvestris, Chthoniobacter flavus, and Gaiella occulta in the rhizosphere of resurrection plants R. serbica and R. nathaliae. Penicillium was the most abundant fungi, while Fusarium and Mucor were specific to R. serbica and Metarhizium and Tolypocladiumu were specific to R. nathaliae. These findings highlight the importance of in vivo experiments to confirm the properties of plant growth-promoting bacteria and the potential of resurrection plant rhizosphere as a valuable source for improving crop drought stress tolerance.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Milka Malesevic, Lazar Gardijan, Marija Miljkovic, Paula M. O'Connor, Nemanja Mirkovic, Branko Jovcic, Paul D. Cotter, Goran Jovanovic, Milan Kojic
Summary: The lactic acid bacterium Lactococcus lactis BGBU1-4 produces a bacteriocin called lactolisterin BU (LBU) which has potent antibacterial activity against Gram-positive pathogens. Additionally, it produces an unknown antibacterial product. It was discovered that LBU breaks down to form a truncated version consisting of 31 amino acids, called LBU1-31, which has 12.5% the antibacterial activity of the full-length LBU. The study also revealed that the N-terminal 24 amino acids of LBU are responsible for antibacterial activity.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Horticulture
Jelena Lozo, Dario Danojevic, Zivko Jovanovic, Zeljka Nenadovic, Djordje Fira, Slavisa Stankovic, Svetlana Radovic
Summary: In this study, the effects of two drought-tolerant Bacillus strains, SS-2.7 and SS-29.2, on four sweet pepper genotypes (133, 274, California Wonder-CalW, and Matica) under water deficiency conditions were examined. The results showed that antioxidant enzyme activity under drought conditions was dependent on genotype and bacterial treatment. It was also observed that different genotypes exhibited varying responses to bacterial treatment under water deficiency. The study highlights the growth-promoting effect of drought-tolerant Bacillus strains on selected pepper genotypes and the genotype-dependent response to biostimulant application, especially under stress conditions.
