Article
Immunology
Fereshteh Ashrafi, Sanaz Ahmadi Ghezeldasht, Mohadeseh Zarei Ghobadi
Summary: The study revealed common gene groups between HTLV-1 and BLV infections that promote viral malignancy by enhancing cell proliferation and overall survival of cancer cells in both human and ovine hosts. The dysregulated genes and pathways may serve as efficient candidates for the therapy of these life-threatening diseases.
MICROBIAL PATHOGENESIS
(2021)
Review
Biochemistry & Molecular Biology
Greta Forlani, Mariam Shallak, Roberto Sergio Accolla, Maria Grazia Romanelli
Summary: Cellular and animal models play important roles in studying HTLV-1-associated diseases; cell models can be used to study the mechanisms of HTLV proteins, while animal models can be used to recapitulate pathological processes and validate the effectiveness of treatment methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Nicolas Ducasa, Daniel Grasso, Paula Benencio, Daniela L. Papademetrio, Mirna Biglione, Fatah Kashanchi, Carolina Berini, Maria Noe Garcia
Summary: HTLV-1, as the etiological agent of ATLL, interacts with host cells through its viral proteins to promote viral replication and T cell transformation by engaging NF-kappa B and autophagy pathways. Additionally, HTLV-1 utilizes the autophagy pathway to achieve its infective goals.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Masooma Rahimzada, Mehri Nahavandi, Mona Saffari, Azam Shafaei, Arman Mosavat, Sanaz Ahmadi Gezeldasht, Nazila Ariaee, Narges Valizadeh, Hossein Rahimi, Seyed Abdolrahim Rezaee, Mohammad Derakhshan
Summary: This study evaluated the interaction between the HTLV-1 virus and host at the gene expression level in HTLV-1-associated malignant disease ATLL. The importance of pyroptosis in disease development and progression was also investigated. The results showed that the expression of CXCR3 and CCR6 was decreased in ATLL patients, while the HTLV-1 proviral load was higher. There was no significant difference in IL-1β expression between ATLL patients and asymptomatic carriers, but there was a meaningful difference in Caspase-1 expression.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Virology
Hengbo Li, Feng Zou, Jie Zhang, Shengyu Zhu, Kaifei Chu, Xu Zhang, Tiejun Zhao
Summary: This study reveals the negative regulatory role of Yes-associated protein (YAP) in Tax activation of HTLV-1 transcription. YAP interacts with Tax and prevents the association between Tax and p300, resulting in the inhibition of HTLV-1 transcription. This finding is important for understanding the mechanisms underlying the persistent infection and latency of HTLV-1 in host cells.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Oncology
Asami Yamada, Jun-ichirou Yasunaga, Lihan Liang, Wenyi Zhang, Junya Sunagawa, Shinji Nakaoka, Shingo Iwami, Yasunori Kogure, Yuta Ito, Keisuke Kataoka, Masanori Nakagawa, Masako Iwanaga, Atae Utsunomiya, Ki-Ryang Koh, Toshiki Watanabe, Kisato Nosaka, Masao Matsuoka
Summary: This study demonstrates that profiling the humoral immunity to HTLV-1 antigens and measuring proviral load can be used to classify disease status and predict the development of HTLV-1-associated diseases. The study also highlights the importance of anti-Gag proteins in disease prognosis.
Review
Biochemistry & Molecular Biology
Shinsuke Nakajima, Kazu Okuma
Summary: This article summarizes the characteristics and applications of animal models of adult T cell leukemia (ATL) caused by human T cell leukemia virus type 1 (HTLV-1) infection, including transgenic and immunodeficient mice. Mouse models play an important role in ATL research, helping to understand the pathogenesis of ATL and develop related treatment methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Aurelie Beauvois, Helene Gazona, Pradeep Singh Chauhan, Majeed Jamakhani, Jean-Rock Jacques, Marc Thiry, Emmanuel Dejardin, Emmanuel Di Valentin, Jean-Claude Twizere, Jean-Marie Peloponese, Makon-Sebastien Njock, Jun-Ichirou Yasunaga, Masao Matsuoka, Malik Hamaidia, Luc Willems
Summary: This study identified HLTF as a restriction factor against HTLV-1 by activating autophagic flux to inhibit viral replication. The viral protein Tax suppresses HLTF transcription and induces proteasomal degradation of HLTF protein. HLTF induction leads to dispersal of the Golgi apparatus and overproduction of secretory granules, resulting in an increase in defective viral particles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Tiejun Zhao, Zhilong Wang, Jinyong Fang, Wenzhao Cheng, Yiling Zhang, Jinhua Huang, Lingling Xu, Hongwei Gou, Linghui Zeng, Zhigang Jin, Masao Matsuoka
Summary: In this study, it was found that YAP activation is associated with HTLV-1 infection in adult T-cell leukemia/lymphoma (ATL), and this activation is mediated by the NF-kappa B/p65 pathway. YAP activation plays a critical role in the pathogenesis of ATL, suggesting YAP as a potential therapeutic target for ATL treatment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Medicine, General & Internal
Michi Miura, Tadasuke Naito, Mineki Saito
Summary: Human T-cell leukemia virus type 1 (HTLV-1) is a replication-competent human retrovirus that is associated with two types of diseases, adult T-cell leukemia-lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Although most infected individuals remain asymptomatic, a subpopulation develops these diseases. The complex virus-host interactions and the host immune response may regulate the development of HTLV-1-associated diseases.
FRONTIERS IN MEDICINE
(2022)
Article
Hematology
Wenbin Zhong, Xiuye Cao, Guoping Pan, Qun Niu, Xiaoqin Feng, Mengyang Xu, Mingchuan Li, Yu Huang, Qing Yi, Daoguang Yan
Summary: This study reveals that ORP4L is expressed in ATL cells and plays an important role in the initiation of T-cell leukemogenesis induced by HTLV-1. Loss of miR-31 leads to increased ORP4L expression, which interacts with PI3Kd to promote PI(3,4,5)P3 generation, resulting in AKT hyperactivation, NF-kappa B-dependent p53 inactivation, and pro-oncogene expression.
Review
Oncology
Kisato Nosaka, Masao Matsuoka
Summary: ATL is a lymphomatous neoplasm caused by HTLV-1 infection, with viral genes and alterations of the host genome playing important roles in leukemogenesis. Expression patterns of HBZ and Tax vary among ATL cases, leading to proposed subtypes. Host immune responses to Tax also contribute to the disease's heterogeneity.
Article
Biochemistry & Molecular Biology
Ji-Lin Chen, Pei-Yi Chu, Chun-Teng Huang, Tzu-Ting Huang, Wan-Lun Wang, Yu-Hsuan Lee, Yuan-Ya Chang, Ming-Shen Dai, Chung-Wai Shiau, Chun-Yu Liu
Summary: This study demonstrates that SHP-1 agonist has potent anti-tumor effects in DLBCL and may serve as a potential therapeutic drug by targeting SHP-1/p-Lyn.
MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Susan Smith, Jaideep Seth, Amanda Midkiff, Rachel Stahl, Yu-Ci Syu, Nikoloz Shkriabai, Mamuka Kvaratskhelia, Karin Musier-Forsyth, Pooja Jain, Patrick L. Green, Amanda R. Panfil
Summary: In this study, YBX1 was found to interact with the viral gene HBZ of HTLV-1 and enhance transcription activity from the 5 & PRIME; LTR promoter. The interaction between YBX1 and Tax or HBZ affected the transcription and protein abundance of both genes. Overall, YBX1 positively influences HTLV-1 gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Virology
Xiaorui Zuo, Ruoning Zhou, Sikai Yang, Guangyong Ma
Summary: HTLV-1 is an oncogenic retrovirus that mainly persists in the infected host cell as a provirus; it also causes a malignancy called adult T-cell leukemia/lymphoma (ATLL) in about 5% of infection. HTLV-1 replication is silent in vivo and relies on clonal proliferation of infected T cells for viral propagation. The mechanism of leukemic clone formation and ATLL onset during HTLV-1 infection is still unclear. This review summarizes the current understanding of HTLV-1 persistence and oncogenesis, incorporating recent cutting-edge discoveries obtained by high-throughput sequencing.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Microbiology
Chieh-Wen Lo, Shin-nosuke Takeshima, Kosuke Okada, Etsuko Saitou, Tatsuo Fujita, Yasunobu Matsumoto, Satoshi Wada, Hidetoshi Inoko, Yoko Aida
Summary: This study revealed that different alleles of BoLA-DRB3 are associated with susceptibility to BLV-induced lymphoma in cattle, with certain amino acid positions showing correlation with lymphoma susceptibility. Additionally, the study showed that the binding pocket of resistance DRB3 is positively charged, while susceptible DRB3 alleles are neutrally charged.
Article
Virology
Ippei Takeuchi, Tadahiro Nasukawa, Ryosuke Sugimoto, Iyo Takemura-Uchiyama, Hironobu Murakami, Jumpei Uchiyama
Summary: The study found that the propagation processes of phages need to be tailored according to the type of phage, and careful consideration should be given to the choice of methodologies for measuring phage concentration.
Article
Microbiology
Iyo Takemura-Uchiyama, Hiroki Tsurui, Hidekatsu Shimakura, Tadahiro Nasukawa, Ichiro Imanishi, Jumpei Uchiyama, Tomoki Fukuyama, Shuji Sakamoto, Keiko Morisawa, Masato Fujimura, Hironobu Murakami, Shuji Kanamaru, Kenji Kurokawa, Keiko Kawamoto, Keita Iyori, Masahiro Sakaguchi
Summary: Staphylococcus pseudintermedius is a major pathogen causing canine skin infection, and it likely exacerbates the severity of canine atopic dermatitis (AD). This study investigated the IgE reactivities to different strains of S. pseudintermedius and identified several IgE-reactive proteins. The findings suggest that S. pseudintermedius may worsen AD in dogs differently from Staphylococcus aureus in human AD.
FEMS MICROBIOLOGY LETTERS
(2022)
Article
Veterinary Sciences
Naoyuki Aihara, Anna Momoki, Nanase Hattori, Hironobu Murakami, Motoharu Oishi, Yoshiaki Goto, Reiichiro Sato, Makoto Nagai, Kazutaka Yamada, Junichi Kamiie
Summary: This is a case of congenital malformations of the external and middle ear accompanied by temporal bone anomaly, characterized by bilateral microtia and absent external acoustic meatus.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2022)
Article
Microbiology
Ayumi Nakatsuchi, Sonoko Watanuki, Liushiqi Borjigin, Hirotaka Sato, Lanlan Bai, Ryosuke Matsuura, Maho Kuroda, Hironobu Murakami, Reiichiro Sato, Sakurako Asaji, Asako Ando, Yasunobu Matsumoto, Shin-Nosuke Takeshima, Yoko Aida
Summary: This study revealed that BoLA-DRB3 polymorphism affects the PVL and infectivity of milk from BLV-infected dams, with susceptible dams showing significantly higher BLV provirus detection rate and PVL compared to resistant dams. The visualization of infectivity using a luminescence syncytium induction assay confirmed a markedly high infectious risk of milk from susceptible dams.
Article
Microbiology
Lanlan Bai, Liushiqi Borjigin, Hirotaka Sato, Shin-Nosuke Takeshima, Sakurako Asaji, Hiroshi Ishizaki, Keiji Kawashima, Yuko Obuchi, Shinji Sunaga, Asako Ando, Hidehito Inoko, Satoshi Wada, Yoko Aida
Summary: This study found that polymorphism in BoLA-DRB3 alleles is associated with bovine leukemia virus (BLV) infection, with susceptible cattle showing stronger BLV infectivity and a strong positive correlation between BLV infectivity and PVL. BLV-infected susceptible cattle were found to be at higher risk of horizontal transmission, while BLV-infected resistant cattle had low transmission risk.
Article
Virology
Yuka Fukase, Fujiko Minami, Tsuneyuki Masuda, Toru Oi, Hitoshi Takemae, Hiroho Ishida, Hironobu Murakami, Naoyuki Aihara, Takanori Shiga, Junichi Kamiie, Tetsuya Furuya, Tetsuya Mizutani, Mami Oba, Makoto Nagai
Summary: This study investigates Japanese porcine orthoreoviruses and finds that they exhibit genetic diversity and potential zoonotic transmission. In addition, intragenetic reassortment and homologous recombination events contribute to the evolution of these viruses.
ARCHIVES OF VIROLOGY
(2022)
Article
Biotechnology & Applied Microbiology
J. Uchiyama, T. Osumi, K. Mizukami, T. Fukuyama, A. Shima, A. Unno, I Takemura-Uchiyama, Y. Une, H. Murakami, M. Sakaguchi
Summary: This study found that atopic dermatitis can alter the oral and gut microbiota in dogs. The diversity of the oral microbiota varied significantly among the dogs, while there was no significant difference in the gut microbiota. The gut microbiota of AD dogs had a differential abundance of the Anaerovoracaceae family, while no specific bacterial taxa were detected in the oral microbiota. Furthermore, the bacteria in the oral microbiota associated with AD were related to human periodontitis, while those in the gut microbiota were associated with dysbiosis and gut inflammation.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Virology
Jumpei Uchiyama, Iyo Takemura-Uchiyama, Kazuyoshi Gotoh, Shin-ichiro Kato, Yoshihiko Sakaguchi, Hironobu Murakami, Tomoki Fukuyama, Mao Kaneki, Osamu Matsushita, Shigenobu Matsuzaki
Summary: This study examined the phylogeny of dU jumbophages, a type of phage with uracil in their DNA. Through gene analysis and gene-sharing network analysis, the researchers found that dU jumbophages are similar to each other and form a clustered group. They also discovered two uncultured phages that may be related to dU jumbophages. These findings suggest that dU jumbophages may have originated from a common source and diverged before the divergence of Gram-negative and Gram-positive bacteria.
Article
Microbiology
Junko Kohara, Lanlan Bai, Shin-nosuke Takeshima, Yuki Matsumoto, Tsunao Hirai, Yoko Aida
Summary: This study experimentally infected seven cattle with BLV and analyzed the distribution of the viral infection in blood and various organs. BLV was first detected in the blood after one week of infection and the viral load increased during the first three weeks. The viral load remained high in five cattle but decreased in two cattle. BLV was found in multiple organs, especially the spleen and lymph nodes, and the amount of BLV in organs was comparable to that in the blood. The findings suggest that the blood proviral load can be used to estimate the distribution of BLV in organs, lymph nodes, and body fluids.
Article
Veterinary Sciences
Sakurako Neo, Iyo Takemura-Uchiyama, Jumpei Uchiyama, Hironobu Murakami, Ayaka Shima, Hideki Kayanuma, Taiki Yokoyama, Satoshi Takagi, Eiichi Kanai, Masaharu Hisasue
Summary: This study analyzed the microbial community in the canine gallbladder and found a specific bacterial community present in the bile of diseased dogs, while no bacteria were detected in healthy dogs.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Mariko Komuro, Masaki Nagane, Tomoki Fukuyama, Xiaolin Luo, Shinobu Hiraki, Masakatsu Miyanabe, Miyuki Ishikawa, Chiaki Niwa, Hironobu Murakami, Mariko Okamoto, Tadashi Yamashita
Summary: The study found that sphingomyelin plays a vital role in maintaining the cutaneous barrier through regulation of the STAT3 pathway. These results suggest that sphingomyelin could be a potential therapeutic target for treating dermatitis.
Article
Veterinary Sciences
Kanumporn Mungthong, Soe Thiri Khaing, Takehiko Otsubo, Chihiro Hatanaka, Shuji Yoneyama, Shin Hisamatsu, Hironobu Murakami, Kenji Tsukamoto
Summary: The P25 primer set was designed for broad detection of BVDV1 and BVDV2 with high sensitivity. Compared to the Aebischer primer set, the P25 primer set can detect a wider range of subgenotypes.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Hirofumi Kawakubo, Shinji Kamisuki, Kei Suzuki, Jesus Izaguirre-Carbonell, Shiki Saito, Hiroshi Murata, Atsushi Tanabe, Ayumi Hongo, Hironobu Murakami, Sachihiro Matsunaga, Kengo Sakaguchi, Hiroeki Sahara, Fumio Sugawara, Kouji Kuramochi
Summary: Sulfoglycolipid, SQAP, is a radiosensitizing agent that increases the sensitivity of tumor cells to radiation therapy. In low oxygen conditions, SQAP shows higher efficacy in inhibiting the growth of hepatocarcinoma cells and affecting the expression of key proteins.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Microbiology
Tadahiro Nasukawa, Ryosuke Sugimoto, Jumpei Uchiyama, Iyo Takemura-Uchiyama, Hironobu Murakami, Ken Fukuda, Shigenobu Matsuzaki, Masahiro Sakaguchi
Summary: In this study, high-performance flow cytometry was used to analyze membrane vesicles (MVs) from Gram-positive bacteria, Staphylococcus aureus and Bacillus subtilis, showing that flow cytometric sorting provided higher purity and uniformity compared to gradient isolation alone. The MV purification method using flow cytometry should be useful for applications requiring very high purity of MV samples such as proteomic, metagenomic, or lipidomic studies.
RESEARCH IN MICROBIOLOGY
(2021)
