Article
Biology
Samantha R. Hartmann, Andrew J. Charnesky, Simon P. Fruh, Robert A. Lopez-Astacio, Wendy S. Weichert, Nadia DiNunno, Sung Hung Cho, Carol M. Bator, Colin R. Parrish, Susan L. Hafenstein
Summary: This study investigates the immune response of dogs to vaccination using cryo-electron microscopy and computational methods. The results reveal a specific polyclonal immune response in the serum of dogs infected with canine parvovirus and provide insights into the binding of multiple antibodies or ligands to the virus.
COMMUNICATIONS BIOLOGY
(2023)
Article
Immunology
Michele Bergmann, Monika Freisl, Katrin Hartmann, Stephanie Speck, Uwe Truyen, Yury Zablotski, Matthias Mayr, Astrid Wehner
Summary: The study found that dogs with levothyroxine-treated HypoT responded to vaccination similarly to healthy dogs, with no significant differences in pre-vaccination antibody levels, vaccination response, or post-vaccination adverse events between the two groups.
Article
Multidisciplinary Sciences
Chady H. Hakim, Sandeep R. P. Kumar, Dennis O. Perez-Lopez, Nalinda B. Wasala, Dong Zhang, Yongping Yue, James Teixeira, Xiufang Pan, Keqing Zhang, Emily D. Million, Christopher E. Nelson, Samantha Metzger, Jin Han, Jacqueline A. Louderman, Florian Schmidt, Feng Feng, Dirk Grimm, Bruce F. Smith, Gang Yao, N. Nora Yang, Charles A. Gersbach, Shi-jie Chen, Roland W. Herzog, Dongsheng Duan
Summary: The study investigates the immune responses induced by AAV-CRISPR therapy in canine models of DMD, indicating that the Cas9-specific T cell response may pose a critical barrier to treatment.
NATURE COMMUNICATIONS
(2021)
Review
Immunology
Cecilia Righi, Giulia Franzoni, Francesco Feliziani, Clinton Jones, Stefano Petrini
Summary: Bovine Alphaherpesvirus 1 (BoHV-1) is a significant respiratory pathogen in cattle worldwide, causing bovine respiratory disease. Immunosuppression initially occurs after infection but is followed by the development of innate and adaptive immune responses. Vaccines targeting both humoral and cell-mediated immunity have been developed. This review summarizes the current understanding of cell-mediated immune responses against BoHV-1 infection and vaccination.
Review
Immunology
Peiyu Zheng, Yongqing Dou, Qinying Wang
Summary: Chronic hepatitis B virus (HBV) infection poses a major threat to global public health, despite increasing vaccination rates. The outcome of HBV infection depends on the interaction between viral replication and host immune response. While innate immunity plays an initial role, long-term immunity is lacking due to HBV's ability to evade detection. Therefore, adaptive immunity involving T cells and B cells is crucial in controlling and clearing HBV infections. However, the persistence of HBV leads to immune tolerance and makes achieving a functional cure challenging.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Food Science & Technology
Cristina Valeria Bulgaru, Daniela Eliza Marin, Gina Cecilia Pistol, Ionelia Taranu
Summary: ZEA is an estrogenic fusariotoxin that affects reproductive health and immune response. It can cause reproductive disorders and impact immune response, with studies showing effects on different types of immune cells and immune organs. Despite the increasing research on the mechanisms of ZEA in modulating immune response, data are currently insufficient and further investigation is needed.
Article
Immunology
Min Joo Choi, Jung Yeon Heo, Yu Bin Seo, Young Kyung Yoon, Jang Wook Sohn, Ji Yun Noh, Hee Jin Cheong, Woo Joo Kim, Ju-yeon Choi, Hwa Jung Kim, Young Jae Lee, Hye Won Lee, Sung Soon Kim, Byoungguk Kim, Joon Young Song
Summary: This study aimed to investigate the humoral and cellular immune response to the mRNA-1273 vaccine through a prospective longitudinal cohort. The results showed that the two-dose mRNA-1273 vaccination induced a strong antibody response that remained elevated over time. A higher peak antibody titer was associated with better cross-reactive immunity against Delta and Omicron variants and longer-lasting humoral immunity. Overall, the cellular immune response was not correlated with antibody titers.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Jacqueline Pearce, Norman Spibey, David Sutton, Ian Tarpey
Summary: A novel recombinant canine parvovirus type 2c vaccine strain has been developed to overcome maternal antibody interference and provide effective immunity against the virus in young puppies. The vaccine, combined with a distemper virus vaccine, has proven to be safe and successful in inducing sterilising immunity in naive animals.
Article
Chemistry, Multidisciplinary
Zhefan Yuan, Bowen Li, Wenchao Gu, Sijin Luozhong, Ruoxin Li, Shaoyi Jiang
Summary: This study presents a new approach to enhance immune tolerance and reduce immunological response to AAV vectors by using an immunosuppressive zwitterionic polypeptide. The modified AAV vectors maintain their transduction efficiency while avoiding the production of AAV-specific antibodies.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Immunology
Simona Arientova, Katerina Matuskova, Oldrich Bartos, Michal Holub, Ondrej Beran
Summary: This study examined the dynamics and duration of immune responses after a three-dose regimen of the BNT162b2 mRNA vaccine. The results showed that the vaccine induced potent and enduring humoral and cellular immune responses, with enhanced protection after the third dose that remained stable up to a year later.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Veterinary Sciences
F. Shams, H. Pourtaghi, Z. Abdolmaleki
Summary: This study investigates the importance of timely vaccination against CPV-2 in puppies and suggests that measuring MDA titers can help reduce the risk of vaccine failure.
BMC VETERINARY RESEARCH
(2022)
Review
Immunology
Vladimir N. Uversky, Elrashdy M. Redwan, William Makis, Alberto Rubio-Casillas
Summary: The effectiveness of the COVID-19 vaccines in preventing severe disease and hospitalization in individuals with comorbidities is being questioned. Recent investigations have found abnormally high levels of IgG4 in individuals who received mRNA vaccines, which may suppress natural antiviral responses and promote SARS-CoV2 infection and replication.
Article
Virology
Robert A. Lopez-Astacio, Oluwafemi F. Adu, Daniel J. Goetschius, Hyunwook Lee, Wendy S. Weichert, Brian R. Wasik, Simon P. Frueh, Brynn K. Alford, Ian E. H. Voorhees, Joseph F. Flint, Sarah Saddoris, Laura B. Goodman, Edward C. Holmes, Susan L. Hafenstein, Colin R. Parrish
Summary: Canine parvovirus (CPV) is a small DNA virus that causes serious diseases in dogs worldwide. In this study, in vitro selection and deep sequencing were used to investigate the escape mutations in CPV induced by two antibodies. The results provide insights into the selection of viral variants and the interactions between antibodies and receptors.
JOURNAL OF VIROLOGY
(2023)
Article
Immunology
Partha Chattopadhyay, Pallavi Mishra, Priyanka Mehta, Jyoti Soni, Rohit Gupta, Bansidhar Tarai, Sandeep Budhiraja, Rajesh Pandey
Summary: The emergence of multiple Spike mutations in SARS-CoV-2 has led to enhanced immune escape, resulting in vaccination breakthrough infections. Transcriptome sequencing of VBT and unvaccinated patients revealed significant differential expression of lncRNAs and mRNAs, with Alu, LINE1, and LTRs playing a role in mediating interactions between lncRNAs and genes within the same TADs. This repeat element-mediated regulation may explain the milder symptoms and shorter hospital stays in VBT cases.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Abigail L. Reens, Damien J. Cabral, Xue Liang, James E. Norton, Alex G. Therien, Daria J. Hazuda, Gokul Swaminathan
Summary: Emerging evidence suggests that the success of immunotherapies can be influenced by the microbiome, and understanding its role in immune-targeted interventions could help predict outcomes and improve efficacy. Key studies have revealed interactions between the microbiome, immune system, and treatment outcomes, particularly in cancer immunotherapy and vaccination, highlighting factors like short-chain fatty acids, MAMPs, and inflammatory cytokines that play a role in microbial immunomodulation. Continued research into these interactions may lead to novel approaches that enhance the efficacy of immune-targeted interventions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Orthopedics
Leo H. Wang, Laura M. Johnstone, Michael Bindschadler, Stephen J. Tapscott, Seth D. Friedman
Summary: This study investigated the effect of immunosuppressive therapy on FSHD patients and found a slight increase in STIR signal, indicating that STIR may not be a dynamic marker for suppressing inflammation in FSHD. Functional outcome measures remained stable throughout the treatment.
BMC MUSCULOSKELETAL DISORDERS
(2021)
Article
Genetics & Heredity
Richard J. L. F. Lemmers, Patrick J. van der Vliet, Ana Blatnik, Judit Balog, Janez Zidar, Don Henderson, Rianne Goselink, Stephen J. Tapscott, Nicol C. Voermans, Rabi Tawil, George W. A. M. Padberg, Baziel G. M. van Engelen, Silvere M. van der Maarel
Summary: This study identified two FSHD families in which the disease is caused by a de novo D4Z4 repeat exchange between chromosomes 4 and 10, leading to repeat contraction and DUX4 expression from chromosome 10. The genetic lesion causal to FSHD in these families is physically separated from other candidate genes on chromosome 4. Muscle cell cultures from affected family members exhibited the characteristic molecular features of FSHD, indicating that DUX4 derepression is the dominant disease pathway for FSHD.
JOURNAL OF MEDICAL GENETICS
(2022)
Article
Orthopedics
Leo H. Wang, Dennis W. W. Shaw, Anna Faino, Christopher B. Budech, Leann M. Lewis, Jeffrey Statland, Katy Eichinger, Stephen J. Tapscott, Rabi N. Tawil, Seth D. Friedman
Summary: This study found that muscles with intermediate baseline fat fraction were more likely to progress in FSHD patients. Functional outcome measures were correlated with overall MRI disease burden but did not show significant changes in the short term. Quantitative strength measurement of the TA revealed a steep loss of strength when the muscle had more than 20% fatty infiltration.
BMC MUSCULOSKELETAL DISORDERS
(2021)
Article
Medicine, Research & Experimental
Zachary M. Howard, Lisa E. Dorn, Jeovanna Lowe, Megan D. Gertzen, Pierce Ciccone, Neha Rastogi, Guy L. Odom, Federica Accornero, Jeffrey S. Chamberlain, Jill A. Rafael-Fortney
Summary: Gene replacement therapy for DMD using micro-dystrophins is being tested in clinical trials, but its effectiveness in preventing heart failure is uncertain. Current animal models are not sufficient to evaluate the efficacy of micro-dystrophins on DMD-related heart failure. A new mouse model has been created to study heart failure progression in DMD and test the effectiveness of micro-dystrophin gene therapy for the first time.
Article
Biochemistry & Molecular Biology
Chao-Jen Wong, Leo Wang, V. Michael Holers, Ashley Frazer-Abel, Silvere M. van der Maarel, Rabi Tawil, Jeffrey M. Statland, Stephen J. Tapscott
Summary: Advances in understanding the pathophysiology of facioscapulohumeral dystrophy (FSHD) have led to the development of therapeutic approaches and the need for biomarkers of disease activity and progression. This study found elevated complement components in plasma from FSHD patients, suggesting the potential use of complement activation measurements as a non-invasive assessment of FSHD disease activity, progression, and response to therapies.
HUMAN MOLECULAR GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Chao-Jen Wong, Jennifer L. Whiddon, Ashlee T. Langford, Andrea E. Belleville, Stephen J. Tapscott
Summary: Mis-expression of DUX4 in skeletal muscle causes facioscapulohumeral muscular dystrophy (FSHD). Dogs have a DUXC gene that is similar to DUX4 and Dux, and the expression of DUXC in canine cells induces a pluripotent program similar to DUX4 and Dux. The conservation of homeodomains and transcriptional program activation between human DUX4 and canine DUXC suggests the potential use of DUXC-containing mammals for preclinical models of FSHD.
HUMAN MOLECULAR GENETICS
(2022)
Article
Multidisciplinary Sciences
Anita van den Heuvel, Saskia Lassche, Karlien Mul, Anna Greco, David San Leon Granado, Arend Heerschap, Benno Kusters, Stephen J. Tapscott, Nicol C. Voermans, Baziel G. M. van Engelen, Silvere M. van der Maarel
Summary: This study investigates FSHD-associated transcriptome signatures in FSHD skeletal muscle biopsies and explores their correlation with various disease-associated factors. It also analyzes the predictive power of imaging-based biomarkers for detecting FSHD signatures in clinical trials. Additionally, the study examines the role of infiltrating non-muscle cell types in FSHD signature expression.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Remko Goossens, Mara S. Tihaya, Anita van den Heuvel, Klorane Tabot-Ndip, Iris M. Willemsen, Stephen J. Tapscott, Roman Gonzalez-Prieto, Jer-Gung Chang, Alfred C. O. Vertegaal, Judit Balog, Silvere M. van der Maarel
Summary: The study identified and characterized novel SMCHD1 interacting proteins, with RUVBL1 being crucial for DUX4 repression in FSHD myocytes. Additionally, the interaction of SMCHD1 with EZHIP provides new insights into its potential function in early embryonic repression of DUX4. The SMCHD1 interactome can guide further research on its function at various genomic loci.
SCIENTIFIC REPORTS
(2021)
Correction
Cell Biology
Sarah B. Crist, Travis Nemkov, Ruth F. Dumpit, Jinxiang Dai, Stephen J. Tapscott, Lawrence D. True, Alexander Swarbrick, Lucas B. Sullivan, Peter S. Nelson, Kirk C. Hansen, Cyrus M. Ghajar
NATURE CELL BIOLOGY
(2022)
Article
Cell Biology
Sarah B. Crist, Travis Nemkov, Ruth F. Dumpit, Jinxiang Dai, Stephen J. Tapscott, Lawrence D. True, Alexander Swarbrick, Lucas B. Sullivan, Peter S. Nelson, Kirk C. Hansen, Cyrus M. Ghajar
Summary: This study reveals that skeletal muscle imposes oxidative stress on disseminated tumour cells (DTCs), which inhibits their proliferation and prevents metastatic colonization. The findings suggest new vulnerabilities of DTCs that can be targeted to prevent metastasis in susceptible tissues.
NATURE CELL BIOLOGY
(2022)
Editorial Material
Medicine, General & Internal
Jeffrey S. Chamberlain
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yee Nip, Sean R. Bennett, Andrew A. Smith, Takako Jones, Peter L. Jones, Stephen J. Tapscott
Summary: Human DUX4 and its mouse ortholog Dux are normally expressed in the early embryo and activate a portion of the first wave of zygotic gene expression. FSHD-causing mutations result in aberrant expression of DUX4 in skeletal muscle, leading to muscle pathology. This study identifies pig DUXC mRNA expressed in early development and shows that both pig DUXC and human DUX4 activate a similar early embryonic program in pig muscle cells, suggesting pig models for FSHD research.
HUMAN MOLECULAR GENETICS
(2023)
Article
Cell Biology
Andrew A. Smith, Yee Nip, Sean R. Bennett, Danielle C. Hamm, Richard J. L. F. Lemmers, Patrick J. van der Vliet, Manu Setty, Silvere M. van der Maarel, Stephen J. Tapscott
Summary: The transcription factor DUX4 plays a role in regulating the early embryonic stem cell program in cancer cells. It is expressed transiently in a small subset of cells, activating the gene expression program associated with early embryonic lineages while suppressing certain proteins. Changes in growth conditions or DNA damage can increase the expression of DUX4.
Article
Biochemistry & Molecular Biology
Danielle C. Hamm, Ellen M. Paatela, Sean R. Bennett, Chao-Jen Wong, Amy E. Campbell, Cynthia L. Wladyka, Andrew A. Smith, Sujatha Jagannathan, Andrew C. Hsieh, Stephen J. Tapscott
Summary: The transcription factor DUX4 regulates translation to change the cellular proteome, and its misexpression is associated with muscular dystrophy and immune evasion in cancer.
Article
Cell Biology
Marcelo dos Santos Voltani Lorena, Estela Kato dos Santos, Renato Ferretti, G. A. Nagana Gowda, Guy L. Odom, Jeffrey S. Chamberlain, Cintia Yuri Matsumura
Summary: By studying the tongue muscles of mdx mice, it was found that the tongue muscles of old mdx mice remain partially spared from severe myonecrosis observed in other muscles. Certain metabolites and proteins have the potential to be used as specific biomarkers for DMD progression independent of aging, while caution should be taken when using tongue muscle metabolites for disease progression monitoring due to age-related changes.
