Article
Microbiology
Stephen C. Watts, Louise M. Judd, Rosemary Carzino, Sarath Ranganathan, Kathryn E. Holt
Summary: Respiratory infection during childhood is a key risk factor in early cystic fibrosis lung disease progression. Haemophilus influenzae and Haemophilus parainfluenzae are commonly isolated from the lungs of children with CF, with a high frequency of colonization and antimicrobial resistance. The genetic basis for AMR in these bacteria was identified, and Haemophilus parainfluenzae had a higher prevalence of acquired AMR genes than H. influenzae.
Article
Immunology
Sara E. Oliver, Amy B. Rubis, Heidi M. Soeters, Arthur Reingold, Meghan Barnes, Susan Petit, Monica M. Farley, Lee H. Harrison, Kathy Como-Sabetti, Sarah A. Khanlian, Rachel Wester, Ann Thomas, William Schaffner, Henju Marjuki, Xin Wang, Susan Hariri
Summary: This study evaluated the epidemiology of NTHi disease in the US, including among pregnant women, infants, and PWH. Results showed that NTHi causes substantial invasive disease, especially among older adults, pregnant/postpartum women, and neonates. Enhanced surveillance and targeted interventions are needed to prevent perinatal NTHi infections.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Biochemical Research Methods
Sam J. Moons, Emiel Rossing, Jurriaan J. A. Heming, Mathilde A. C. H. Janssen, Monique van Scherpenzeel, Dirk J. Lefeber, Marien de Jonge, Jeroen D. Langereis, Thomas J. Boltje
Summary: The study explores the structure-activity relationship of sialic acid-based inhibitors that prevent the transfer of host sialic acids to Nontypeable Haemophilus influenzae. Small modifications are tolerated at the C-5 and C-9 positions, while the C-8 position does not allow for modification. These findings define the chemical space available for developing selective bacterial sialylation inhibitors.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Pediatrics
Grigorios Chatziparasidis, Ahmad Kantar, Keith Grimwood
Summary: The respiratory tract antimicrobial defense system protects the lungs from microorganisms through various defense mechanisms. Nontypeable Haemophilus influenzae (NTHi), a potential pathogen, uses multiple strategies to colonize the lower airways and establish persistent infections. NTHi impairs mucociliary clearance, evades host defenses, and forms biofilms, causing chronic infections and inflammation. Understanding the molecular pathogenesis of NTHi is important for developing effective therapies and vaccines, considering its genetic heterogeneity. Currently, there are no vaccine candidates ready for large clinical trials.
PEDIATRIC PULMONOLOGY
(2023)
Article
Nutrition & Dietetics
Elliot Mathieu, Quentin Marquant, Florian Chain, Edwige Bouguyon, Vinciane Saint-Criq, Ronan Le-Goffic, Delphyne Descamps, Philippe Langella, Thomas A. A. Tompkins, Sylvie Binda, Muriel Thomas
Summary: Microbiota studies have shown that certain strains of Streptococcus have potential antimicrobial properties against respiratory pathogens. In this study, a Streptococcus mitis strain from a human newborn was found to have in vitro antimicrobial activity against non-typable Haemophilus influenza, Pseudomonas aeruginosa, and Staphylococcus aureus. However, further research is needed to determine its efficacy in protecting against respiratory infections, as treatment with this strain did not provide effective protection against NTHi lung infection and may have even exacerbated the infection.
Article
Microbiology
Nadav Topaz, Raymond Tsang, Ala-Eddine Deghmane, Heike Claus, Thien-Tri Lam, David Litt, Maria Paula Bajanca-Lavado, Maria Perez-Vazquez, Didrik Vestrheim, Maria Giufre, Arie Van Der Ende, Olivier Gaillot, Alicja Kuch, Martha McElligott, Muhamed-Kheir Taha, Xin Wang
Summary: Invasive diseases caused by Haemophilus influenzae serotype a (Hia) have been rising in North America and Europe. Genomic analysis identified four genetically distinct clades, with some clades mainly composed of isolates from North America or Europe. Capsule duplication was found in certain isolates, while the presence of known antibiotic resistance factors was low.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Pediatrics
Lin Yang, YaFei Zhu, GuangSheng Wu
Summary: The purpose of this study is to improve the clinical understanding of acute empyema caused by Haemophilus influenzae by analyzing the clinical data of a child with this condition and conducting a literature review. The study found that comprehensive treatment, including antibiotics, closed pleural drainage, and intrapleural injection of urokinase, effectively controlled the patient's condition.
FRONTIERS IN PEDIATRICS
(2022)
Article
Infectious Diseases
Anne Sofie Froekiaer Mathiasen, Lene Hyldgaard Bigum, Kim Hovgaard Andreassen, Hans-Christian Slotved, Dahlia Caroline Adjal Berring, Valeria Antsupova
Summary: This article presents a rare case of urosepsis caused by Haemophilus influenzae, a pathogen with specific growth requirements that should be considered in the diagnostic process. The patient was young and had nephrocalcinosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Immunology
Ying-Chun Chien, Yu-Tsung Huang, Chun-Hsing Liao, Jung-Yien Chien, Po-Ren Hsueh
Summary: This study investigated the clinical characteristics and outcomes of bacteremia caused by Haemophilus and Aggregatibacter species in patients treated at a medical center between 2006 and 2018. The results showed that the majority of patients with bacteremia had community-acquired disease, with a higher Sequential Organ Failure Assessment (SOFA) score being an independent predictor of poor 7-day clinical outcomes in patients with community-acquired H. influenzae bacteremia.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Pediatrics
Marika Paalanne, Minna Honkila, Niko Paalanne, Suvi Mattila, Tytti Pokka, Marjo Renko, Terhi Tapiainen
Summary: This study investigated the social burden of nasopharyngeal detection of different respiratory viruses and the co-detection of viral and bacterial pathogens. It was found that co-detection of any respiratory virus and Streptococcus pneumoniae or Haemophilus influenzae in the nasopharynx was associated with longer parental absence from work compared to the detection of viruses alone.
Article
Immunology
Xiao Wu, Runfeng Li, Yunceng Weng, Hongxia Zhou, Haiming Jiang, Jin Zhao, Bin Liu, Ruifeng Chen, Xinxin Chen, Weimin Yang, Zifeng Yang, Xinhua Wang
Summary: Primary influenza virus infection can predispose hosts to secondary infection with Haemophilus influenzae, which increases disease severity and mortality. Sequential infection with influenza A virus and Haemophilus influenzae induced lethal synergy in a mouse model, possibly due to increased bacterial loads and lung damage. Analysis showed a correlation between specific adhesion molecules and bacterial growth in secondary pneumonia following primary viral infection.
MICROBES AND INFECTION
(2021)
Article
Immunology
Janet R. Gilsdorf
Summary: Hib vaccines are crucial in preventing serious infections in children, with conjugated vaccines offering enhanced immunogenicity and prolonged protection. Global incidence of Hib infections has significantly decreased due to widespread vaccination efforts.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Microbiology
Jake G. Natalini, Shivani Singh, Leopoldo N. Segal
Summary: New methods and technologies have revealed that the human lungs are not sterile, but have a distinct lung microbiome with low biomass and dynamic microbial immigration. Understanding the microbial ecology of the lung is crucial for identifying new therapeutic targets and improving lung health.
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Nadia A. Kadry, Eric A. Porsch, Hao Shen, Joseph W. St Geme
Summary: NTHi is a common cause of respiratory tract disease and preventing colonization is a strategy to prevent disease. Immunization with HMW1 and HMW2 can protect against colonization by both parent NTHi strain and heterologous strains expressing distinct HMW1 and HMW2 proteins. The protection against heterologous strains is IL-17A dependent.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Environmental Sciences
Shaocheng Zhang, Xi Chen, Jing Wang, Chunmei Dai, Yeran Gou, Huanhuan Wang
Summary: Particulate air pollution is associated with respiratory Heamophilus influenzae infection. High pollution and infection rates are observed in winter, while low rates are seen in summer. PM2.5 is an independent risk factor for infection, as shown in single-pollutant model analysis.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Editorial Material
Microbiology
Brian J. Akerley
Article
Immunology
Sandy M. Wong, Jutamas Shaughnessy, Sanjay Ram, Brian J. Akerley
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2016)
Article
Multidisciplinary Sciences
Wenchao Li, Xinyun Zhang, Ying Yang, Qingqin Yin, Yan Wang, Yong Li, Chuan Wang, Sandy M. Wong, Ying Wang, Howard Goldfine, Brian J. Akerley, Hao Shen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Immunology
Sandy M. S. Wong, Frank St. Michael, Andrew Cox, Sanjay Ram, Brian J. Akerley
INFECTION AND IMMUNITY
(2011)
Article
Immunology
Charles V. Rosadini, Jeffrey D. Gawronski, Daniel Raimunda, Jose M. Argueello, Brian J. Akerley
INFECTION AND IMMUNITY
(2011)
Article
Immunology
Charles V. Rosadini, Sanjay Ram, Brian J. Akerley
INFECTION AND IMMUNITY
(2014)
Article
Multidisciplinary Sciences
Sandy M. Wong, Mariana Bernui, Hao Shen, Brian J. Akerley
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Article
Immunology
Sandy M. Wong, Mary Darby Jackson, Brian J. Akerley
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2019)
Article
Immunology
Mary Darby Jackson, Sandy M. Wong, Brian J. Akerley
INFECTION AND IMMUNITY
(2019)
Article
Microbiology
Angela H. Benton, Mary Darby Jackson, Sandy M. Wong, Justine L. Dees, Brian J. Akerley, Mary E. Marquart
Article
Immunology
Mary Darby Jackson, Sandy M. Wong, Brian J. Akerley
Summary: NTHi utilizes sialic acid to evade host immune defenses and sialylation protects NTHi glycan structures from IgM and complement-mediated killing. Sialylation of LOS reduced the survival of siaB mutant in the murine lung due to decreased IgM binding, highlighting the importance of this evasion strategy in NTHi infection.
INFECTION AND IMMUNITY
(2021)
Article
Immunology
Jorge E. Vidal, Meagan N. Wier, Uriel A. Angulo-Zamudio, Erin McDevitt, Ana G. Jop Vidal, Babek Alibayov, Anna Scasny, Sandy M. Wong, Brian J. Akerley, Larry S. McDaniel
Summary: The study found that deoxycholic acid can kill all tested strains of Streptococcus pneumoniae within a short period of time and has an eradication effect on its colonization in the nasopharynx. The mechanism of action of deoxycholic acid does not involve autolysis and is not effective against oral streptococcal species.
INFECTION AND IMMUNITY
(2021)
Article
Immunology
Sandy M. Wong, Jeffrey Gawronski, Brian J. Akerley
Summary: Copper is toxic at high concentrations, but its role in pathogenesis and mechanisms of copper resistance in Haemophilus influenzae are not well understood. In this study, we found that the ATPase gene copA and the metallochaperone gene copZ are involved in copper homeostasis in H. influenzae. Deletion of these genes increased sensitivity to copper but not to other metals. We also showed that the expression of the copZA operon is activated by copper and regulated by CueR. Mutants with deletions in copA and copZ genes exhibited decreased copper tolerance and higher copper accumulation. These findings suggest that the cop system plays an important role in alleviating copper toxicity and maintaining copper homeostasis in H. influenzae.
INFECTION AND IMMUNITY
(2023)
Article
Microbiology
Yong Li, Ying Yang, Dafan Chen, Yan Wang, Xinyun Zhang, Wenchao Li, Shengsen Chen, Sandy M. M. Wong, Mengwen Shen, Brian J. Akerley, Hao Shen
Summary: Streptococcus pneumoniae (Sp) frequently causes secondary pneumonia after influenza A virus (IAV) infection, leading to high morbidity and mortality worldwide. Concomitant pneumococcal and influenza vaccination improves protection against coinfection but does not always yield complete protection. In this study, we showed that preceding low-dose IAV infection caused persistent Sp infection and suppression of bacteria-specific T-helper type 17 (Th17) responses in mice. Prior Sp infection protected against subsequent IAV/Sp coinfection by improving bacterial clearance and rescuing bacteria-specific Th17 responses in the lungs. Furthermore, blockade of IL-17A by anti-IL-17A antibodies abrogated the protective effect of Sp preinfection. Importantly, memory Th17 responses induced by Sp preinfection overcame viral-driven Th17 inhibition and provided cross-protection against different Sp serotypes following coinfection with IAV. These results indicate that bacteria-specific Th17 memory cells play a key role in providing protection against IAV/Sp coinfection in a serotype-independent manner and suggest that a Th17-based vaccine would have excellent potential to mitigate disease caused by coinfection.