Article
Environmental Sciences
Gerhard P. Aigner, Pamela Nenning, Birgit Fiechtner, Maja Srut, Martina Hoeckner
Summary: This study found that the demethylating agent Aza inhibits gene activity while Cd induces MT2 gene expression, but neither has an effect on global DNA methylation levels. Thus, Cd detoxification based on MT2 induction is not related to changes in DNA methylation.
Article
Genetics & Heredity
Yanlong Zhao, Xiaorong Zeng, Xinli Xu, Wenjing Wang, Lei Xu, Yiying Wu, Hang Li
Summary: The aim of this study was to investigate the effect of the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (Aza) on early renal injury. The results showed that low-dose Aza attenuated apoptosis and inflammation, and improved renal function. Furthermore, low-dose Aza upregulated the expression of Klotho protein, and the inhibition of Klotho protein reversed the protective effects of low-dose Aza on apoptosis and inflammation.
Article
Oncology
Huiying Cheng, Sijie Tang, Xueqi Lian, Hong Meng, Xiang Gu, Jiajia Jiang, Xiaohua Li
Summary: DNA methylation, mediated by DNA methyltransferase, affects gene expression and plays a role in malignant diseases. The classic inhibitor 5-Aza inhibits tumor cell proliferation and induces cell cycle arrest. It increases tumor suppressive proteins and transcription factors, while decreasing oncogene expression. These results suggest potential clinical applications in prostate cancer treatment.
Article
Agronomy
Shuo Liu, Yu Bao, Hui Deng, Guanqing Liu, Yangshuo Han, Yuechao Wu, Tao Zhang, Chen Chen
Summary: DNA methylation is a crucial epigenetic modification that regulates gene expression and genome stability. The methylation inhibitor, AzaD, can effectively reduce genome-wide hypomethylation and cause a series of epigenetic variations.
Article
Biochemistry & Molecular Biology
Karolina Luczkowska, Piotr Kulig, Klaudia Rusinska, Bartlomiej Baumert, Boguslaw Machalinski
Summary: This study investigates the effects of a methylation inhibitor on the proliferation of multiple myeloma cells and the development of resistance to treatment, finding that alterations in DNA methylation are associated with drug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Xiaoli Zhang, Gang Wang, Hui Li, Xiangming Jiang, Xiaoyong Zhao
Summary: The study demonstrated that 5-AZA-DC can alleviate the symptoms of preeclampsia by modulating the expression and interaction of STAT3, PTEN, and TSC2. It also promotes cell proliferation, inhibits apoptosis, and relieves the physiological abnormalities induced by PE.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
Article
Immunology
Shramana M. Banerjee, Pilar Acedo, Soha El Sheikh, Rania Harati, Amelia Meecham, Norman R. Williams, Gareth Gerard, Mohammed R. S. Keshtgar, Alexander J. Macrobert, Rifat Hamoudi
Summary: Functional experiments showed that combining 5-ADC and PDT had a synergistic effect in vitro and increased cytotoxicity in TNBC 4T1 tumor cells. Treatment with 5-ADC/PDT showed histopathologically defined margins of necrosis and increased T cell expression, indicating an anti-tumor immune response. Bioinformatics analysis identified altered immune response biomarkers in TNBC compared to other breast cancer subtypes.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Isis Salviano Soares de Amorim, Juliana Alves Rodrigues, Pedro Nicolau, Sandra Konig, Carolina Panis, Adenilson de Souza da Fonseca, Andre Luiz Mencalha
Summary: This study investigated the molecular effects of DNA methyltransferase inhibitor in response to treatment in normoxia and hypoxia in breast cancer model. It was found that the inhibitor upregulated proinflammatory pathways, with a greater effect observed in normoxia than hypoxia. Specifically, NF-kappa B expression increased only in normoxia after treatment with the inhibitor, suggesting a potential context-dependent impact on inflammatory response in the tumor microenvironment.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Oncology
Yang-Jie Zhang, Ran Xie, Jie Jiang, Li Zhai, Cong-Hui Yang, Jing Zhang, Xi Wang, Dong-Xue Chen, Hua-Tao Niu, Long Chen
Summary: The study demonstrates the methylation modification of GAS5 in melanoma and the reversible effect of 5-Aza-dC on this modification. Treatment with 5-Aza-dC inhibits cell proliferation, increases apoptosis, and reduces migration and invasion in melanoma cells. The antitumor effect of 5-Aza-dC in melanoma is related to the methylation modification of GAS5.
Article
Biochemistry & Molecular Biology
Alain Aguirre-Vazquez, Luis A. Salazar-Olivo, Xochitl Flores-Ponce, Ana L. Arriaga-Guerrero, Dariela Garza-Rodriguez, Maria E. Camacho-Moll, Ivan Velasco, Fabiola Castorena-Torres, Nidheesh Dadheech, Mario Bermudez de Leon
Summary: By utilizing epigenetic molecules and small molecules, the expression of pluripotency genes can be induced and methylation in the OCT4 promoter can be reduced in human somatic cells, enhancing reprogramming efficiency. Further studies are needed to elucidate the mechanisms by which these compounds exert their effects on reprogramming.
Review
Pharmacology & Pharmacy
William B. Parker, Jaideep V. Thottassery
Summary: DNMT1 depletion is a rational goal in DNA methylation-dependent disorders, and current agents like decitabine and azacitidine have off-target activities limiting their use. Aza-T-dCyd is a promising candidate with less toxicity and higher therapeutic index for selectively depleting DNMT1. Further research is needed to understand its mechanism of action and potential clinical applications.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2021)
Article
Multidisciplinary Sciences
Reem Miari, Naiel Azzam, Rinat Bar-Shalom, Fuad Fares
Summary: 5-Aza-2'-deoxycytidine has shown effectiveness in treating HPV(-) HNSCC by inhibiting proliferation and inducing apoptosis. Future studies are needed to clarify the molecular mechanism of action of 5-AzaD in HPV(-) cancer cells.
Article
Biology
Amir Ali Khan, Muhammad Nasir Khan Khattak, Divyasree Parambath, Ahmed Taher El-Serafi
Summary: Our study demonstrated that 5-aza inhibits adipogenic differentiation of MG-63 cells, while SAHA enhances it. Genome-wide expression analysis revealed high variability within samples and distinct expression profiles for cells derived with 5-aza. Differential analysis identified numerous DE genes and transcription factors across different pairwise comparisons. Additionally, GO terms related to enhanced adipogenesis and up-regulation of certain signaling pathways were enriched in 5-aza treated cells. Further research is required to understand the roles of these genes in adipogenesis.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Z. Feng, R. Meng, Q. Li, D. Li, Q. Xu
Summary: The study revealed that AZA modulated the LTA-induced inflammatory response through the NF-κB signaling pathway in hOBs, enhancing the production of specific inflammatory cytokines. Additionally, AZA had a significant impact on the NF-κB and MAPK signaling pathways in LTA-stimulated hOBs, further confirming the roles of these pathways in hOBs treated with LTA.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Article
Oncology
Gordon Greville, Esther Llop, Jane Howard, Stephen F. Madden, Antoinette S. Perry, Rosa Peracaula, Pauline M. Rudd, Amanda McCann, Radka Saldova
Summary: This study demonstrates that the DNA methyltransferase inhibitor 5-AZA-dC can increase branching and sialylation of N-glycans in cancer cells, and regulates glycosyltransferases through GATA transcription factors. Treated cells, especially in chemo-sensitive cell lines, showed increased migration and apoptotic markers. The findings provide insight into the impact of epigenetic alterations on cancer cell glycosylation.
CLINICAL EPIGENETICS
(2021)
Article
Oncology
Srimanta Patra, Soumya R. Mishra, Bishnu P. Behera, Kewal K. Mahapatra, Debasna P. Panigrahi, Chandra S. Bhol, Prakash P. Praharaj, Gautam Sethi, Samir K. Patra, Sujit K. Bhutia
Summary: Autophagy is a self-degradation process that eliminates dysfunctional cytoplasmic cargos to maintain cellular homeostasis. It plays a dual role in cytoprotection and cytostasis during malignant transformation, and is regulated by phyto-products. Autophagy also regulates epigenetic modulation and inflammation, limiting tumor metastasis.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Oncology
Dipta Sengupta, Moonmoon Deb, Swayamsiddha Kar, Nibedita Pradhan, Sabnam Parbin, R. Kirtana, Suraj P. Singh, S. G. Suma, Niharika, Ankan Roy, Saumen Manna, Priyanka Saha, Priyanka Chakraborty, Soumya Dash, Chahat Kausar, Samir Kumar Patra
Summary: miRNAs play a key role in regulating biological processes and development in animals and plants, they are involved in post-transcriptional gene regulation by preventing translation. Aberrant regulation of miRNAs is associated with cancer development, understanding the biogenesis and function of miRNAs could offer potential therapeutic implications for treating breast cancers.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Oncology
Soumya Ranjan Mishra, Kewal Kumar Mahapatra, Bishnu Prasad Behera, Chandra Sekhar Bhol, Prakash Priyadarshi Praharaj, Debasna Pritimanjari Panigrahi, Srimanta Patra, Amruta Singh, Shankargouda Patil, Rohan Dhiman, Samir Kumar Patra, Sujit Kumar Bhutia
Summary: Inflammasomes play a critical role in tumor development, and their components have significant effects on tumorigenesis in a tissue-specific and stage-dependent manner. The proinflammatory cytokines produced by inflammasome activation can regulate tissue homeostasis and induce antitumor immune responses, but they can also promote cancer growth and proliferation by directing oncogenic signaling pathways in cancer cells. Epigenetic mechanisms and autophagy control inflammasome activation and contribute to cancer development.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Prajna Paramita Naik, Subhadip Mukhopadhyay, Prakash Priyadarshi Praharaj, Chandra Sekhar Bhol, Debasna Pritimanjari Panigrahi, Kewal Kumar Mahapatra, Srimanta Patra, Sarbari Saha, Aditya Kumar Panda, Krupasindhu Panda, Subhankar Paul, Palok Aich, Samir Kumar Patra, Sujit Kumar Bhutia
Summary: Clusterin induces autophagy in oral cancer through the AMPK/Akt/mTOR signaling pathway, protecting cells from apoptosis, interacts with ULK1 to induce autophagy, and promotes mitophagy in nutrient deprivation conditions. High CLU expression is associated with therapy resistance and clusterin-mediated mitophagy inhibits cell death by cisplatin in cancer cells.
Review
Genetics & Heredity
Samir Kumar Patra
Summary: Gene expression is crucial for cellular functions and homeostasis, with histone modifications playing a role in regulating gene expression. Recent studies have shown how tissue transglutaminase 2 mediated histone glutamine modifications can regulate gene expression, opening new avenues in the field of epigenetics and neuroepigenetics. The research aims to identify enzymes responsible for reversible reactions of histone de-dopaminylation and de-serotonylation.
Review
Biochemistry & Molecular Biology
Prakash Priyadarshi Praharaj, Srimanta Patra, Debasna Pritimanjari Panigrahi, Samir Kumar Patra, Sujit Kumar Bhutia
Summary: Clusterin (CLU) is a significant cancer regulator that controls various cancer-associated cellular events, making it an ideal target for cancer therapy. Inhibition of CLU enhances the anticancer potential of chemotherapy drugs and improves patient survival.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2021)
Article
Biochemistry & Molecular Biology
Chandra Sekhar Bhol, Soumya Ranjan Mishra, Shankargouda Patil, Sunil Kumar Sahu, R. Kirtana, Soumen Manna, Muthu Kumaraswamy Shanmugam, Gautam Sethi, Samir Kumar Patra, Sujit Kumar Bhutia
Summary: Procaine inhibits DNA methylation by suppressing DNMT activity and increases the expression of PAX9 in oral squamous cell carcinoma cells, triggering an anticancer mechanism and potentially offering a new therapeutic strategy for OSCC.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Chemistry, Inorganic & Nuclear
Rupam Dinda, Sudarshana Majumder, Monalisa Mohanty, Deepika Mohapatra, Sushree Aradhana Patra, Rakesh Parida, Santanab Giri, Hans Reuter, Chahat Kausar, Samir Kumar Patra
Summary: The synthesis and interaction between three new dioxidomolybdenum(VI) complexes and aroylhydrazone ligands containing azobenzene moiety have been reported. The structures and properties of the ligands and complexes were characterized, and the redox properties of the complexes were studied. The molecular structures and electronic spectra of the complexes were determined using X-ray crystallography and time dependent density functional theory. The complexes were found to interact with DNA and exhibit cytotoxic activity.
Article
Biochemistry & Molecular Biology
Samir Kumar Patra, Moshe Szyf
Summary: COVID-19 has caused global devastation, yet many aspects of the virus' behavior and impact on the body remain unknown. This article focuses on the role of cellular metabolism and epigenetic mechanisms in the progression of the disease.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Environmental Sciences
Ranjay Kumar Thakur, Mukesh Singh, Samir Patra, Prasanta Kumar Biswas, Atanu Bhowmik
Summary: In this study, fruit peels waste was collected from a residential and fruit-juice center in Purba Medinipur district of West Bengal, India. The powder generated from these wastes was analyzed, and it was found to have heterogeneous particles with a diameter ranging from 10 to 55 μm and a moisture content of approximately 4%. Various minerals and 33 bioactive molecules were detected in the powder. Different extracts were prepared from the powder using solvents and showed high antioxidant properties. Methanol, ethanol, petroleum ether, and butanol extracts exhibited cytotoxic potential against Pseudomonas aeruginosa MZ269380, and all extracts were biocompatible with goat's erythrocytes at MIC concentration. The biovalorization of fruit peels can produce useful phytochemicals for biomedical, food processing, and cosmetics industries.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Biochemistry & Molecular Biology
Soumen Manna, R. Kirtana, Ankan Roy, Tirthankar Baral, Samir Kumar Patra
Summary: Understanding the molecular mechanism(s) of small compounds in cellular growth control is crucial for combating diseases. Oral cancers have a high mortality rate due to their metastatic potential. Aberrant EGFR, RAR, HH signaling, elevated [Ca2+] and oxidative stress are important characteristics of oral cancer. Therefore, we targeted these features for our study. We tested the effects of fendiline hydrochloride (FH), erismodegib (a SMO inhibitor), and all-trans retinoic acid (RA) on cellular differentiation.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Review
Genetics & Heredity
Soumen Manna, Jagdish Mishra, Tirthankar Baral, R. Kirtana, Piyasa Nandi, Ankan Roy, Subhajit Chakraborty, Samir K. Niharika, Samir K. Patra
Summary: Chromatin modifications, such as DNA methylation, histone modification, and recruitment of noncoding RNAs, play essential roles in epigenetic events. These modifications converge into a complex epigenetic landscape, involving the recognition of promoter DNA methylation by MeCP2/methyl CpG binding domain proteins and the recruitment of SETDB1/SUV39 to establish higher order chromatin structure. While many studies have provided new information on different epigenetic modifications and associated factors, clear maps of interconnected pathways are also emerging. This review focuses on the significant crosstalk mechanisms utilized by cells for various cellular processes and how deregulation or aberrant gene expression contributes to disease progression.
Article
Oncology
Swayamsiddha Kar, Niharika, Ankan Roy, Samir Kumar Patra
Summary: SOX2 plays a critical role in tumor development, cancer stem cell maintenance, and cancer progression. The mechanisms of SOX2 gene regulation in human breast and prostate cancers are still unknown. This study found that both prostate and breast cancer tissues showed high expression of SOX2, and inhibiting SOX2 significantly decreased cell proliferation and migration. Epigenetic modulators enhanced SOX2 expression, and histone modifications were crucial for the overexpression of SOX2 during tumorigenesis. Co-targeting SOX2 and its active epigenetic modifier enzymes could be a potential strategy for effectively treating aggressive prostate and breast cancers.
Review
Cell & Tissue Engineering
Ankan Roy, Samir Kumar Patra
Summary: Over the past fifty years, there have been changing views on the organization and dynamics of the plasma membrane at the molecular level. Lipid rafts, nanoscale subdomains enriched with cholesterol and sphingolipids, play a unique role among other subdomains by dynamically harboring signaling proteins and orchestrating multiple signaling pathways. They are involved in cellular physiology, homeostasis, embryonic development, stem cell maintenance, and cancer biology.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Cell & Tissue Engineering
Prajna Paramita Naik, Swagatika Panigrahi, Ratnakar Parida, Prakash Priyadarshi Praharaj, Chandra Sekhar Bhol, Shankargouda Patil, N. M. L. Manjunath, Dipanjan Ghosh, Samir Kumar Patra, Sujit Kumar Bhutia
Summary: Cancer stem cells (CSCs) are a rare subset of malignant cells with stem cell-like features that acquire stemness through metabolic rewiring and epigenetic reprogramming, influencing tumor progression. Specific drugs targeting the metaboloepigenetic circuitry of CSCs may serve as potential cancer therapeutics.
STEM CELL REVIEWS AND REPORTS
(2022)
