Journal
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Volume 71, Issue -, Pages 477-482Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2053230X15005178
Keywords
NDH-II; NADH dehydrogenase; respiratory chain; Staphylococcus aureus
Funding
- Fundacao para a Ciencia e a Tecnologia [SFRH/BPD/79224/2011, SFRH/BPD/80741/2011, PTDC/BIA-PRO/118535/2010, PTDC/BBB-BQB/2294/2012, PEst-OE/EQB/LA0004/2011]
- Bio-Struct-X [1493]
- Fundação para a Ciência e a Tecnologia [PTDC/BBB-BQB/2294/2012, PTDC/BIA-PRO/118535/2010, SFRH/BPD/79224/2011] Funding Source: FCT
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In recent years, type II NADH dehydrogenases (NDH-IIs) have emerged as potential drug targets for a wide range of human disease causative agents. In this work, the NDH-II enzyme from the Gram-positive human pathogen Staphylococcus aureus was recombinantly expressed in Escherichia coli, purified, crystallized and a crystallographic data set was collected at a wavelength of 0.873 angstrom. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 81.8, b = 86.0, c = 269.9 angstrom, contained four monomers per asymmetric unit and diffracted to a resolution of 3.32 angstrom. A molecular-replacement solution was obtained and model building and refinement are currently under way.
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