Journal
BIOMED RESEARCH INTERNATIONAL
Volume 2018, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2018/4349170
Keywords
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [15H04677, 26560225, 15K12524, 15K21444, 16K18993, 15J10205]
- Japan Heart Foundation
- Waseda University [2016 K-225]
- Grants-in-Aid for Scientific Research [15H04677, 16K18993, 15K21444, 15J10205, 15K12524, 26560225] Funding Source: KAKEN
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The present study was conducted to systematically investigate the optimal viral titer as well as the volume of the adenovirus vector (ADV) that expresses alpha-actinin-AcGFP in the Z-disks of myocytes in the left ventricle (LV) of mice. An injection of 10 mu L ADV at viral titers of 2 to 4 x 10(11) viral particles per mL (VP/mL) into the LV epicardial surface consistently expressed alpha-actinin-AcG FP in myocytes in vivo, with the fraction of AcG FP-expressing myocytes at similar to 10%. Our analysis revealed that SI, was similar to 1.90-2.15 mu m upon heart arrest via deep anesthesia. Likewise, we developed a novel fluorescence labeling method of the T-tubular system by treating the LV surface with CellMask Orange (CellMask). We found that the T-tubular distance similar to 2.10-2.25 mu m, similar to SL, in the healthy heart in vivo. Therefore, the present high-precision visualization method for the Z-disks or the T-tubules is beneficial to unveiling the mechanisms of myocyte contraction in health and disease in vivo.
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