Article
Chemistry, Multidisciplinary
Cong Yao, Zhennan Qiu, Xiao Li, Hui Zhu, Dichen Li, Jiankang He
Summary: Electrohydrodynamic (EHD) printing is used to fabricate microfibrous architectures for cellular orientation, but orderly deposition of microfibers with cell-scale spacing is challenging. A finite element method is established to analyze the electrostatic forces induced on the EHD-printed microfibers, and it is found that uniform fiber arrangement can be achieved when the fiber spacing is five times larger than the fiber diameter. Decreasing the fiber spacing improves neurite outgrowth length and Schwann cell migration.
Article
Materials Science, Biomaterials
Jiaqi Fang, Xuehan Jin, Bo Xu, Liping Nan, Shuhao Liu, Jianguang Wang, Na Niu, Zhong Wu, Feng Chen, Junjian Liu
Summary: Chlorogenic acid (CGA) is a polyphenol with high bioactivity and therapeutic potential for various diseases. This study investigated the role of CGA in promoting peripheral nerve regeneration. A biodegradable conduit containing CGA-releasing microspheres was successfully prepared and used for repairing sciatic nerve defects, demonstrating favorable effects on functional recovery.
BIOMATERIALS SCIENCE
(2023)
Article
Engineering, Biomedical
Tabitha N. Rosenbalm, Nicole H. Levi, Michael J. Morykwas, William D. Wagner
Summary: In this study, bioresorbable materials capable of producing electric field gradients were developed to assess their impact on neuronal cell growth and migration. The results showed that the biphasic conductive composites can maintain nerve mechanical properties while promoting neuronal cell growth. Tubular conduits composed of these materials were found to be superior to silicone conduits for nerve repair.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2023)
Article
Endocrinology & Metabolism
Vuong M. Pham, Prashanth Komirishetty, Aparna Areti, Trevor Poitras, Nitish Thakor, Douglas W. Zochodne
Summary: This study suggests that insulin signaling may have additive impacts on neuropathy independent of glycemia and PTEN knockdown. The findings show that insulin can promote survival of neurons and proliferation of Schwann cells. In a chronic mouse model of diabetic polyneuropathy, local PTEN inhibition and insulin injections had synergistic effects on correcting key features of the disease. Overall, this suggests that local PTEN inhibition and insulin can provide beneficial trophic support for sensory neurons and Schwann cells in the treatment of diabetic polyneuropathy without requiring metabolic correction.
Article
Neurosciences
Anda Mann, Bibiane Steinecker-Frohnwieser, Aida Naghilou, Flavia Millesi, Paul Supper, Lorenz Semmler, Sonja Wolf, Lena Marinova, Lukas Weigl, Tamara Weiss, Christine Radtke
Summary: This study investigated the effect of low nuclear magnetic resonance therapy (NMRT) on cells involved in peripheral nerve regeneration. The results showed that NMRT stimulated Schwann cell proliferation and promoted dorsal root ganglion neuron regeneration. Furthermore, the medium obtained from NMRT treated Schwann cell cultures supported neuron survival and neurite outgrowth.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Cell Biology
Aurelia A. Mapps, Michael B. Thomsen, Erica Boehm, Haiqing Zhao, Samer Hattar, Rejji Kuruvilla
Summary: In this study, five different populations of satellite glia from sympathetic and sensory ganglia were identified using single-cell RNA sequencing analysis. Furthermore, sensory and sympathetic-specific satellite glia were found to have different molecular characteristics.
Article
Cell & Tissue Engineering
Pierre C. Dromel, Deepti Singh, Eliot Andres, Molly Likes, Motoichi Kurisawa, Alfredo Alexander-Katz, Myron Spector, Michael Young
Summary: The biomaterial-based cell replacement approach using gelatin-hyaluronic acid IPN shows promising potential in enhancing intravitreal retinal cell therapy. It improves cell viability, promotes regeneration, and reduces immune cell response, making it a viable treatment option worth further exploration and application in the future.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Engineering, Biomedical
Nora Hlavac, Deanna Bousalis, Raffae N. Ahmad, Emily Pallack, Angelique Vela, Yuan Li, Sahba Mobini, Erin Patrick, Christine E. Schmidt
Summary: This study investigated the impact of low-level electrical stimulation on the regenerative potential of adipose-derived stem cells, revealing that frequency and duration significantly affect the expression of growth factors. Continuous exposure to 1 Hz pulses for 24 hours was found to optimally promote the expression of neural growth factors and induce significant increase in neurite extension.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
D. Merryweather, S. R. Moxon, A. J. Capel, N. M. Hooper, M. P. Lewis, P. Roach
Summary: Cellular metabolism and behaviour are closely linked to cytoskeletal tension and scaffold mechanics. In this study, a 3D culture system using diluted type I collagen hydrogel to produce variation in gel stiffness was explored. The results showed that hydrogel scaffold remodelling was linked to gel collagen concentration and different concentrations of gel led to significant changes in programmed cell behaviour and cell morphology.
Article
Biology
Jinbin Zhai, Hyukmin Kim, Seung Baek Han, Meredith Manire, Rachel Yoo, Shuhuan Pang, George M. Smith, Young-Jin Son
Summary: In mice, eliminating myelin inhibitors and CSPGs together only slightly increased regeneration of conditioned-lesioned DR axons across the DREZ, while their absence significantly enhanced regeneration of GDNF-stimulated axons, emphasizing the importance of elevating intrinsic growth capacity. This suggests that myelin inhibitors and CSPGs are not the primary mechanisms stopping axons at the DREZ.
Review
Engineering, Biomedical
Bradyn J. Parker, David I. Rhodes, Carmel M. O'Brien, Andrew E. Rodda, Neil R. Cameron
Summary: NGCs for peripheral nerve repairs have potential for enhancing nerve regeneration outcomes, but consideration of factors such as regulatory clearance, reimbursement processes, manufacturability and quality management are essential for successful translation of new technologies from bench to clinic.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Kathrin Baumgartner, Sophie C. F. Mauritz, Sebastian Angermann, Manuel S. Brugger, Christoph Westerhausen
Summary: This research examines the impact of SSAW on the neurite outgrowth of B35 cells and explores the applicable range of SSAW parameters. The findings indicate that SSAW treatment significantly enhances the growth of neurites in preferential directions.
Review
Materials Science, Biomaterials
Chun-Yi Yang, Wei-Yuan Huang, Liang-Hsin Chen, Nai-Wen Liang, Huan-Chih Wang, Jiaju Lu, Xiumei Wang, Tzu-Wei Wang
Summary: During nervous system development, the extracellular matrix (ECM) plays a crucial role in neuronal maturation through surface topography and microenvironment signals. Mimicking the microenvironment to promote neural cell adhesion and growth may be a key strategy for neural tissue regeneration.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Review
Materials Science, Biomaterials
Pooja Sharma, Vijay Kumar Pal, Sangita Roy
Summary: Neural tissue engineering shows great potential in functional brain recovery with self-assembling peptides being of interest for their physiochemical properties. Short peptide-derived bioactive hydrogels are easily synthesized and highly tunable. Despite being in early stages, peptides in neural tissue engineering have injectability and wide biomedical applications.
BIOMATERIALS SCIENCE
(2021)
Article
Cell Biology
Daniela F. Duarte Campos, Siyuan Zhang, Franziska Kreimendahl, Marius Koepf, Horst Fischer, Michael Vogt, Andreas Blaeser, Christian Apel, Marcella Esteves-Oliveira
CONNECTIVE TISSUE RESEARCH
(2020)
Article
Anatomy & Morphology
Anja Boeckers, Horst Claassen, Kirsten Haastert-Talini, Jurgen Westermann
Summary: In this viewpoint, representatives of the Teaching Commission of the Anatomical Society summarize their teaching experiences during the COVID-19 pandemic and recommend universities to prioritize face-to-face teaching while also using online teaching as necessary. The transition to remote learning during the pandemic revealed technical shortcomings and deficits in practical hands-on teaching.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2021)
Review
Neurosciences
Svenja Kankowski, Claudia Grothe, Kirsten Haastert-Talini
Summary: This review discusses neuropathic pain development and handling. The first part describes the pathways of neuropathic pain and different in vivo models. The second part presents the knowledge about cellular and molecular mechanisms that may lead to neuropathic pain. Established animal models can simulate the diversity of neuropathic pain observed in clinics.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Marwa El Soury, Benedetta Elena Fornasari, Giacomo Carta, Federica Zen, Kirsten Haastert-Talini, Giulia Ronchi
Summary: Peripheral nerves are easily prone to injuries and require supportive strategies to stimulate nerve growth and functional recovery. The impact of diet on health and body function is crucial, with balanced nutrition being essential for the health of the central and peripheral nervous systems. Many dietary supplements have shown to play a significant role in peripheral nerve development and regeneration, emphasizing the importance of tailored dietary plans for patients with nerve injuries in promoting nerve regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Zhong Huang, Svenja Kankowski, Ella Ertekin, Mara Almog, Zvi Nevo, Shimon Rochkind, Kirsten Haastert-Talini
Summary: The study found that hydrogel luminal fillers can play a positive role in nerve regeneration, but functional recovery still falls short compared to autologous nerve grafts. In addition, M-HAL showed improvement in the performance of different types of hollow nerve guides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Oleksandr Gryshkov, Fedaa Al Halabi, Antonia Isabel Kuhn, Sara Leal-Marin, Lena Julie Freund, Maria Forthmann, Nils Meier, Sven-Alexander Barker, Kirsten Haastert-Talini, Birgit Glasmacher
Summary: PVDF and P(VDF-TrFE) are promising biomaterials for nerve regeneration, with piezoelectric properties stimulating cell growth. The study compared their structural and piezoelectric properties, using a dynamic impact test machine. PVDF showed increased beta-phase content post-electrospinning and similar zeta potential to P(VDF-TrFE), while also demonstrating guiding properties for nerve outgrowth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Veterinary Sciences
Magdalena A. Czubala, Eva Eilles, Andreas Staubi, Natacha Ipseiz, Michael Vogt, Leonie Zieglowski, Lisa Ernst, Rene H. Tolba, Philip R. Taylor, Ralf Weiskirchen
Summary: The research team has proposed a digital platform called '3R Blackboard' to facilitate the sharing of surplus biological materials from animals, aiming to reduce the number of animals used. Pilot study has shown that this platform is highly effective, resulting in a significant reduction in the number of animals used, as well as resource saving, knowledge exchange, and establishment of new collaborations.
LABORATORY ANIMALS
(2022)
Review
Biochemistry & Molecular Biology
Luisa Muratori, Federica Fregnan, Monica Maurina, Kirsten Haastert-Talini, Giulia Ronchi
Summary: Peripheral nerve injuries can lead to loss of motor and sensory function, and current methods for nerve regeneration have limited success. A potential approach for enhancing peripheral nerve regeneration is through improving diet and lifestyle, and consuming polyphenols.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Instruments & Instrumentation
Federica De Lorenzi, Larissa Yokota Rizzo, Rasika Daware, Alessandro Motta, Maike Baues, Matthias Bartneck, Michael Vogt, Marc van Zandvoort, Leonard Kaps, Qizhi Hu, Marielle Thewissen, Luca Casettari, Cristianne J. F. Rijcken, Fabian Kiessling, Alexandros Marios Sofias, Twan Lammers
Summary: This study explores the use of cyclic arginine-glycine-aspartic acid (cRGD) peptide-decorated polymeric micelles for tumor-targeted drug delivery. The results show that decoration density, incubation time and temperature, and the expression levels of the alpha(v)beta(3) integrin receptor on target cells all influence the uptake of cRGD-decorated polymeric micelles by the target cells.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Jeffrey Momoh, Dimitrios Kapsokalyvas, Michael Vogt, Sjoerd Hak, Fabian Kiessling, Marc van Zandvoort, Twan Lammers, Alexandros Marios Sofias
Summary: Intravital microscopy (IVM) has broad applications in various fields, including biology, physiology, immunology, and drug development. This review provides an overview of the modalities, methods, and model organisms used in IVM, as well as its applications in nanomedicine and biological process studies.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Editorial Material
Neurosciences
Kirsten Haastert-Talini
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Zhong Huang, Rebecca Powell, Svenja Kankowski, James B. Phillips, Kirsten Haastert-Talini
Summary: Researchers have successfully generated Schwann cells from human induced pluripotent stem cells by modifying existing protocols, resulting in sufficient cell numbers. They also found that specific coating and glucose levels are crucial for improving the differentiation process and increasing the yield of viable cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sonia Calabro, Svenja Kankowski, Matilde Cescon, Giovanna Gambarotta, Stefania Raimondo, Kirsten Haastert-Talini, Giulia Ronchi
Summary: It is widely recognized that the gut microbiota plays a crucial role in human health, and its disruption is associated with various diseases. Recent studies have expanded our understanding of the relationship between the gut microbiota and the peripheral nervous system (PNS), highlighting that the PNS can be affected by GM perturbations. This review provides a comprehensive summary of the current knowledge on the impact of the gut microbiota on the PNS, considering both its somatic and autonomic divisions in physiological, regenerative, and pathological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Ramin Nasehi, Ali T. Abdallah, Marcella Pantile, Carlo Zanon, Michael Vogt, Stephan Ruetten, Horst Fischer, Sanja Aveic
Summary: The lack of in vitro cell models is a challenge for discovering new molecular targets and therapeutics for pediatric bone metastatic disease. Using a beta-tricalcium phosphate (β-TCP) multicellular 3D in vitro bone microtissue model, the study successfully reconstitutes key phenotypic and transcriptional patterns of native metastatic tumor cells and enhances their stemness and proinvasive features. The geometry and chemical impact of β-TCP reveals a prometastatic landscape of the tumor microenvironment, providing insights for further analysis of the disease and preclinical target validations.
MATERIALS TODAY BIO
(2023)
Article
Materials Science, Biomaterials
Sanja Aveic, Simon Janssen, Ramin Nasehi, Max Seidelmann, Michael Vogt, Marcella Pantile, Stephan Ruetten, Horst Fischer
Summary: The geometry of synthetic beta-tricalcium-phosphate structures was found to impact the interaction between neuroblastoma tumor cells and stromal components. Growth conditions in a 3D environment are crucial for determining cell proliferation rates, with channeled 3D structures stimulating more cytokine production.
BIOMATERIALS SCIENCE
(2021)