Article
Biochemistry & Molecular Biology
Hironori Mizutani, Yoshifumi Sato, Masaya Yamazaki, Tatsuya Yoshizawa, Yukio Ando, Mitsuharu Ueda, Kazuya Yamagata
Summary: This study found that the expression of SIRT7 is increased in the brain of Alzheimer's disease patients. Treatment with A beta(42) increased the expression of NOX4, leading to the production of reactive oxygen species (ROS) and apoptosis. Knockdown of SIRT7 inhibited A beta(42)-induced ROS production and apoptosis by suppressing the upregulation of NOX4. These findings suggest that inhibiting SIRT7 may be beneficial in the pathogenesis of AD by regulating ROS production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lukas Gola, Laura Bierhansl, Julia Csatari, Christina B. Schroeter, Lisanne Korn, Venu Narayanan, Manuela Cerina, Sara Abdolahi, Anna Speicher, Alexander M. Hermann, Simone Koenig, Albena T. Dinkova-Kostova, Tawfeeq Shekh-Ahmad, Sven G. Meuth, Heinz Wiendl, Ali Gorji, Matthias Pawlowski, Stjepana Kovac
Summary: Hyperexcitability is associated with neuronal dysfunction, cellular death, and neurodegeneration. NOX4, an NADPH oxidase, is found to regulate reactive oxygen species (ROS) levels and calcium homeostasis, thereby preventing hyperexcitability and neuronal death. These findings suggest that NOX4 might have a crucial role in neurodegenerative diseases by acting as a potential redox regulator.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Juan-Hua Quan, Fei Fei Gao, Jia-Qi Chu, Guang-Ho Cha, Jae-Min Yuk, Weiyun Wu, Young-Ha Lee
Summary: Silver nanoparticles (AgNPs) demonstrate anticancer effects against colorectal cancer through inducing mitochondrial reactive oxygen species production, mitochondrial dysfunction, and endoplasmic reticulum stress responses, leading to cell apoptosis.
Review
Oncology
Cecilia H. Kim, Heather A. Leitch
Summary: Iron overload-induced reactive oxygen species affect cellular activities, leading to dysfunction in hematopoietic stem cells and other cells, exacerbating the pathophysiology of myelodysplastic syndromes.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ruifeng Ji, Dou Wu, Qiang Liu
Summary: ICA has been found to inhibit the expression of genes and molecules related to osteoclastogenesis and reduce reactive oxygen species production in induced osteoclasts. These findings suggest that ICA has the potential to be used as a therapeutic agent for osteolytic diseases such as osteoporosis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Hongxiang Jiang, Fei Li, Linzhi Cai, Qianxue Chen
Summary: NOX4 plays a role in angiogenesis in glioblastoma through ROS production, and TSPO is an upstream target of NOX4-derived mitochondrial ROS. The TSPO-NOX4 signaling pathway could serve as a potential molecular target for therapeutic strategies in glioblastoma.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Flavia Rezende, Pedro Felipe Malacarne, Niklas Mueller, Birgit Rathkolb, Martin Hrabe de Angelis, Katrin Schroeder, Ralf P. Brandes
Summary: The study found that Nox4 knockout mice exhibited similar weight loss and sodium sparing function under a low sodium diet, but showed reduced systolic blood pressure, heart-rate ratio, and heart to body weight ratio compared to wild-type mice, indicating that Nox4 plays a role in blood pressure regulation induced by low sodium diets.
Review
Immunology
Miranda D. Chavez, Hubert M. Tse
Summary: Mitochondrial dysfunction and oxidative stress are associated with T cell-mediated autoimmune diseases, and targeting metabolic pathways can inhibit autoreactive T cell activation. Increasing the requirements of Tregs for ROS and oxidative phosphorylation can promote self-tolerance and inhibit the activity of autoreactive T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ali A. A. Akhiani, Anna Martner
Summary: Activation of NADPH oxidases (NOX) and the resulting formation of reactive oxygen species (ROS) play important roles in antimicrobial defense and oncogenesis. Enhanced NOX activity has been linked to the activation of oncogenic cascades such as the phosphoinositide 3-kinase (PI3K) signaling pathway, which is upregulated in various malignancies. This review examines the role of PI3K in regulating NOX-induced ROS formation in cancer.
Review
Medicine, Research & Experimental
Yawei Bi, Xiao Lei, Ningli Chai, Enqiang Linghu
Summary: NOX4 is upregulated in pancreatic cancer and primarily produces hydrogen peroxide, exhibiting different properties from other NOX family subtypes. Recent studies suggest that NOX4 promotes pancreatic cancer occurrence and development, making it a potential therapeutic target in pancreatic cancer.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ildiko Szanto
Summary: Cancer cells can adapt to hypoxic environments through metabolic rewiring and synthesis of antioxidant molecules. NOX4, a member of the NOX family enzymes, plays a key role in the oncogenic metabolic adaptation of cancer cells by regulating diverse metabolic processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Gang Liu, Qingbai Liu, Bin Yan, Ziqiang Zhu, Yaozeng Xu
Summary: OA is a common joint disease that affects middle-aged to elderly people, current treatment options for which are limited. Research has shown that USP7 plays a role in promoting OA progression by regulating NOX4 activity in the ROS/NLPR3 axis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, General & Internal
Huan Li, Fang Hu, Robert Peter Gale, Mikkael A. Sekeres, Yang Liang
Summary: Myelodysplastic syndromes (MDS) are a group of blood cancers characterized by dysregulated hematopoiesis and risk of transformation to acute myeloid leukemia. Prognostic systems can predict survival in MDS patients. Treatment goals differ for low-risk and high-risk MDS, with the aim to improve quality of life and prolong survival, respectively. Hematopoietic cell transplantation can cure MDS, but it is not widely used.
NATURE REVIEWS DISEASE PRIMERS
(2022)
Article
Biochemistry & Molecular Biology
Anton G. Rogov, Tatiana N. Goleva, Khoren K. Epremyan, Igor I. Kireev, Renata A. Zvyagilskaya
Summary: Mitochondria are considered the primary source of ROS in cells, with ROS-induced ROS release triggered in cardiac myocytes under excessive oxidative stress. Using Dipodascus magnusii yeast cells, it was found that prooxidant-induced oxidative stress begins in mitochondria before spreading to the whole cell, with mitochondrial ROS leading to mitochondrial fragmentation.
Review
Oncology
Yan Jiang, Su-Jun Gao, Benoit Soubise, Nathalie Douet-Guilbert, Zi-Ling Liu, Marie-Berengere Troadec
Summary: Gene variants, particularly TP53 mutations, play a crucial role in the prognosis of myelodysplastic syndromes (MDS), being associated with higher risk categories, resistance to traditional therapies, rapid leukemic transformation, and poor outcomes. Current prognosis classification systems for MDS do not consider gene variants, but the impact is significant on the clinical heterogeneity and prognosis of the disease.
Article
Dermatology
Francesca Beretti, Laura Bertoni, Francesca Farnetani, Cristina Pellegrini, Greta Gorelli, Anna Maria Cesinaro, Luca Reggiani Bonetti, Lucia Di Nardo, Shaniko Kaleci, Johanna Chester, Caterina Longo, Daniela Massi, Maria C. Fargnoli, Giovanni Pellacani
EXPERIMENTAL DERMATOLOGY
(2019)
Article
Cell Biology
Jessika Bertacchini, Laura Mediani, Francesca Beretti, Marianna Guida, Aram Ghalali, Federica Brugnoli, Valeria Bertagnolo, Emanuel Petricoin, Francesco Poti, Jessica Arioli, Laura Anselmi, Alessia Bari, James McCubrey, Alberto M. Martelli, Lucio Cocco, Silvano Capitani, Sandra Marmiroli
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Manuela Zavatti, Francesca Beretti, Francesca Casciaro, Emma Bertucci, Tullia Maraldi
Article
Chemistry, Analytical
Valentina Marassi, Francesca Beretti, Barbara Roda, Andrea Alessandrini, Paolo Facci, Tullia Maraldi, Andrea Zattoni, Pierluigi Reschiglian, Marinella Portolani
ANALYTICA CHIMICA ACTA
(2019)
Review
Biochemistry & Molecular Biology
Cristina Angeloni, Martina Gatti, Cecilia Prata, Silvana Hrelia, Tullia Maraldi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Cell Biology
Francesca Casciaro, Michela Borghesan, Francesca Beretti, Manuela Zavatti, Emma Bertucci, Matilde Yung Follo, Tullia Maraldi, Marco Demaria
MECHANISMS OF AGEING AND DEVELOPMENT
(2020)
Article
Cell Biology
Martina Gatti, Manuela Zavatti, Francesca Beretti, Daniela Giuliani, Eleonora Vandini, Alessandra Ottani, Emma Bertucci, Tullia Maraldi
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2020)
Article
Biochemistry & Molecular Biology
Martina Gatti, Francesca Beretti, Manuela Zavatti, Emma Bertucci, Soraia Ribeiro Luz, Carla Palumbo, Tullia Maraldi
Summary: The study found that EV secreted by human amniotic fluid stem cells can improve the differentiation ability of pre-osteoblasts, increase cell viability, and reduce apoptotic markers. Moreover, EV positively modulates the oxidative stress induced by dexamethasone.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Francesca Beretti, Francesca Farnetani, Luca Reggiani Bonetti, Luca Fabbiani, Manuela Zavatti, Antonino Maiorana, Giovanni Pellacani, Tullia Maraldi
Summary: This study reveals a correlation between high positivity for Nox4 and c-met in BRAF mutated patients with melanoma, and metastasis occurring at least one year later than diagnosis. In vitro experiments demonstrate that the HGF/c-met/Nox4/ROS axis triggers epithelial-mesenchymal transition. The findings suggest that Nox4 inhibitors could be a potential addition to current therapies for treating melanoma patients with BRAF mutations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Manuela Zavatti, Martina Gatti, Francesca Beretti, Carla Palumbo, Tullia Maraldi
Summary: This study found that exosomes derived from human amniotic fluid stem cells can alleviate inflammatory injury caused by microglia and significantly recover neurotoxicity. This suggests that hAFSC-exo may serve as a potential therapeutic agent for inflammation-related neurological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Francesca Casciaro, Francesca Beretti, Martina Gatti, Giuseppe Persico, Emma Bertucci, Marco Giorgio, Tullia Maraldi
Summary: Human amniotic fluid cells (hAFSCs) are a heterogeneous population with stem cell characteristics. This study compares the gene expression profiles of adherent amniotic fluid cells and their c-Kit(+) subpopulation through single-cell and bulk RNAseq analysis. Differences in cellular distribution, pathways related to stemness, and secretome composition were identified between the two populations. Selecting c-Kit positive fractions with higher potential could be important in regenerative medicine applications.
Article
Biochemistry & Molecular Biology
Martina Gatti, Katarina Stoklund Dittlau, Francesca Beretti, Laura Yedigaryan, Manuela Zavatti, Pietro Cortelli, Carla Palumbo, Emma Bertucci, Ludo van den Bosch, Maurilio Sampaolesi, Tullia Maraldi
Summary: Neuromuscular junctions are important for communication between spinal motor neurons and skeletal muscle, and their vulnerability in degenerative diseases like muscle atrophy is poorly understood. Recent studies have shown the regenerative potential of stem cells and extracellular vesicles in muscle fiber regeneration, but their role in counteracting NMJ perturbations is not clear. In this study, a co-culture system was used to investigate the effects of AFSC-derived EVs on NMJ alterations induced by muscle atrophy. The presence of EVs reduced morphological and functional defects and prevented oxidative stress in atrophic myotubes. This study provides a valuable tool for studying MN and myotube interactions and demonstrates the efficacy of AFSC-EVs in counteracting NMJ perturbations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)