Review
Biotechnology & Applied Microbiology
Hussein M. El-Husseiny, Eman A. Mady, Walaa A. El-Dakroury, Ahmed S. Doghish, Ryou Tanaka
Summary: Recent advancements in biomedicine and tissue regeneration have had a positive impact on healthcare as a whole, particularly in the field of revitalizing damaged tissues. Cardiac tissue engineering (CTE) using biomolecules, biomimetic scaffolds, and cells is crucial in this process. Stimuli-responsive hydrogels (SRHs) are excellent biomaterials for tissue engineering, as they can mimic the characteristics of natural tissues and act as effective delivery platforms. This review outlines various strategies used in CTE, highlights the limitations of conventional hydrogels, and discusses the characteristics, assembly, and exploitation of SRHs in CTE. Overall, the development of SRHs holds great potential for the reconstruction of degenerated cardiac tissues.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Nanoscience & Nanotechnology
Mingyu Lee, Min Chul Kim, Jae Young Lee
Summary: Cardiovascular disease is a leading cause of death, and biomaterial-based tissue engineering approaches have shown promise in cardiac repair. This review focuses on the use of nanomaterial-incorporated conductive hydrogels for improving cardiac function post-myocardial infarction.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Review
Pharmacology & Pharmacy
Sonia Trombino, Federica Curcio, Roberta Cassano, Manuela Curcio, Giuseppe Cirillo, Francesca Iemma
Summary: Cardiac regeneration aims to reconstruct the heart contractile mass and prevent functional deterioration by delivering pro-regenerative cells, drugs, or growth factors to the injury site. Biomaterials, including engineered hydrogels and polymeric nanoparticles, have shown promise in post-infarction therapy and tissue engineering for cardiovascular diseases. Italian research groups have made significant contributions in this field, focusing on natural and synthetic biomaterials, fabrication techniques, and the current status of clinical trials.
Review
Chemistry, Multidisciplinary
Aleksandra Benko, Thomas J. Webster
Summary: Cardiovascular diseases have significant impact on the worldwide healthcare system, environment, and pharmaceutical, biotechnological, and biomedical companies. There is an urgent need to develop new environmentally-friendly strategies that promise cardiac tissue regeneration and offer solutions for drug screening to avoid cardiovascular toxicity.
FRONTIERS IN CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Jie Qiu, Xiang-Ju Liu, Bei-An You, Na Ren, Hong Liu
Summary: Cardiovascular disease is a leading cause of disability and death worldwide. Stem cell-based therapies, although promising, face challenges such as low transplantation efficiency. Nanomaterials play a crucial role in improving cardiac repair and regeneration through regulating stem cell differentiation. This study discusses the current status, limitations, and recent advances in nanomaterials used in cardiac therapy, as well as the potential clinical applications and future research directions.
Article
Biochemistry & Molecular Biology
Kaveh Roshanbinfar, Tilman U. Esser, Felix B. Engel
Summary: Heart failure is a leading cause of morbidity worldwide with a high mortality rate. Recent advances in utilizing acellular constructs and stem-cell-derived cardiomyocytes have shown promise in improving cardiac function. However, challenges remain in generating vascularized cardiac patches with clinically relevant dimensions and achieving proper electrical coupling between engineered and host tissue for preventing arrhythmia. Future directions include utilizing novel materials and biofabrication strategies to generate prevascularized three-dimensional multicellular constructs for improved cardiac tissue engineering.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Review
Engineering, Biomedical
Yimeng Li, Leqian Wei, Lizhen Lan, Yaya Gao, Qian Zhang, Hewan Dawit, Jifu Mao, Lamei Guo, Li Shen, Lu Wang
Summary: Myocardial infarction (MI) is a fatal disease with increasing incidence worldwide. Limited regenerative capacity of cardiomyocytes leads to scar tissue formation and impaired electrical conduction. Conductive biomaterials have been extensively studied for myocardial repair. This review provides an overview of conductive biomaterials, fabrication methods, in vitro myocardial tissue construction, and in vivo cardiac repair using conductive patches.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Multidisciplinary
Priyadarshi Chakraborty, Hadas Oved, Darya Bychenko, Yifei Yao, Yiming Tang, Shai Zilberzwige-Tal, Guanghong Wei, Tal Dvir, Ehud Gazit
Summary: This di-Fmoc-based hydrogelator containing the RGD fragment exhibits good cell adhesion capacity and achieves semiconductivity by incorporating polyaniline into the hydrogel network, demonstrating excellent antibacterial activity and DNA binding capacity while supporting the growth of functional synchronized monolayers of cardiac cells.
ADVANCED MATERIALS
(2021)
Review
Engineering, Biomedical
Kamil Elkhoury, Margaretha Morsink, Laura Sanchez-Gonzalez, Cyril Kahn, Ali Tamayol, Elmira Arab-Tehrany
Summary: Natural hydrogels are highlighted as promising biomaterials in tissue engineering applications due to their biocompatibility and biodegradability. Innovative biofabrication techniques, such as textile techniques and 3D bioprinting, play a significant role in developing advanced scaffolds for tissue engineering. This review also discusses the emerging applications of biofabricated natural hydrogels in cardiac, neural, and bone tissue engineering.
BIOACTIVE MATERIALS
(2021)
Article
Engineering, Biomedical
Kaveh Roshanbinfar, Maria Kolesnik-Gray, Miriam Angeloni, Stefan Schruefer, Maren Fiedler, Dirk W. Schubert, Fulvia Ferrazzi, Vojislav Krstic, Felix B. Engel
Summary: Cardiac tissue engineering is a promising strategy to prevent heart failure, but several issues remain unsolved. This study develops a biohybrid hydrogel that enhances the beating properties of engineered cardiac tissues and allows for concurrent drug release.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Sonali Sudhir Sali, Maree L. Gould, Muhammad Qasim, M. Azam Ali
Summary: This study investigates the potential of casein as a biomaterial for tissue engineering through chemical modification and combination with other polymers. Results show that the modified casein scaffold exhibits good cell adhesion and biodegradability, promoting cell proliferation. The study not only provides insights into the mechanism of casein methacrylation, but also highlights the use of waste materials like DAF-casein in tissue engineering applications.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Biotechnology & Applied Microbiology
Tatyana Isayeva Waldrop, Caleb Graham, William Gard, Kevin Ingle, Travis Ptacek, Nguyen Nguyen, Bailey Lose, Palaniappan Sethu, Timmy Lee
Summary: Cardiovascular events are the leading cause of death in dialysis patients. Researchers have developed a three-dimensional cardiac tissue chip that can mimic the hemodynamic changes associated with arteriovenous fistula (AVF) creation. By subjecting the tissue constructs to volume overload conditions, they observed fibrosis and gene expression changes similar to those seen in AVF mice.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Plant Sciences
Ritesh Ghosh, Adelin Barbacci, Nathalie Leblanc-Fournier
Summary: Plants have the ability to remember events associated with environmental fluctuations through priming, allowing them to cope with future stressors more efficiently. Mechanical stimulation enhances plants' basal defence responses and stress tolerance, making it a potential priming method for sustainable agriculture.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Polymer Science
Kaylee Meyers, Bruce P. Lee, Rupak M. Rajachar
Summary: Myocardial infarction can cause permanent damage to the heart, leading to cardiac failure. Current transplantation methods face issues such as donor shortages and complications. Polymeric scaffolds offer an alternative, but mechanical property mismatch and limited electrical conductivity are challenges to be addressed for clinical applicability.
Review
Biochemistry & Molecular Biology
Karine Tadevosyan, Olalla Iglesias-Garcia, Manuel M. Mazo, Felipe Prosper, Angel Raya
Summary: Cardiac tissue engineering is a promising strategy for cardiac disease modeling, cardiotoxicity testing, and cardiovascular repair, but challenges remain in replicating tissue-like properties and in assessing functionality and maturation state. Progress has been made in the field over the last two decades, but there is still a need for a unified framework for comparison of engineered cardiac tissue constructs generated using different methodologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Physical
Marco Govoni, Leonardo Vivarelli, Alessandro Mazzotta, Cesare Stagni, Alessandra Maso, Dante Dallari
Summary: Over the past two decades, various options for orthopaedic implants have been developed to meet the increasing demand in the medical and market sectors. Commercially available bone graft products can be classified into three main categories, including cellular bone matrices, growth factor enhanced bone grafts, and peptide enhanced xeno-hybrid bone grafts. Data was collected from search engines and bibliographic databases to analyze the safety, efficacy, and properties of these products in preclinical/clinical studies, with peptide enhanced xeno-hybrid bone grafts showing the most promising risk/benefit ratio but requiring further controlled studies for routine clinical use.
Review
Biochemistry & Molecular Biology
Marco Govoni, Francesca Danesi
Summary: This scoping review provides an overview of the therapeutic potential of hydrolyzable tannins (HTs) and their metabolites in preventing or alleviating the course of osteoarthritis (OA). Although further clinical trials are needed to unravel the potential benefits of these compounds, these studies offer a new dietary strategy for improving OA.
Article
Biotechnology & Applied Microbiology
Pasqualina Scala, J. Lovecchio, E. P. Lamparelli, R. Vitolo, V Giudice, E. Giordano, C. Selleri, L. Rehak, N. Maffulli, G. Della Porta
Summary: An in-vitro model of human bone marrow mesenchymal stem cells (hBM-MSCs) myogenic commitment was developed using static co-seeding and perfused culture systems. Static co-seeding showed a significant increase in gene expression, while the myogenic induction ability of hSkMs on hBM-MSCs under perfusion conditions was mainly exerted through paracrine effect. The dynamic culture also resulted in a significant increase in anti-inflammatory cytokine gene expression, confirming the immunomodulatory activity of hBM-MSCs along with commitment events.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
J. Lovecchio, V Betti, M. Cortesi, E. Ravagli, S. Severi, E. Giordano
Summary: Monitoring the maturation of bone tissue engineered constructs is crucial for ensuring their quality. Traditional destructive methods require sacrificing samples, while light spectroscopy-based methods provide non-destructive monitoring. In this study, a miniaturized and inexpensive custom-made spectrometer device is proposed for real-time/in-line analysis and integration with bioreactor systems.
ROYAL SOCIETY OPEN SCIENCE
(2022)
Article
Chemistry, Physical
Joseph Lovecchio, Marilisa Cortesi, Marco Zani, Marco Govoni, Dante Dallari, Emanuele Giordano
Summary: 3D printing has provided new opportunities for the fabrication of biocompatible hybrid pseudo-tissues. This study focuses on modifying a commercial 3D printer into a 3D bioplotter to control scaffold fiber thickness and porosity. The results demonstrate the feasibility of printing hydrogels commonly used for cell culture and tissue engineering applications.
Article
Medicine, General & Internal
Leonardo Vivarelli, Marco Govoni, Dario Attala, Carmine Zoccali, Roberto Biagini, Dante Dallari
Summary: By simulating the machining of massive bone allografts using different machines, the study showed that the robot outperformed the four-axes machine. This research paves the way for cleanroom machining of massive bone allografts, but further studies are needed.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Pietro Cimatti, Isabella Andreoli, Maurizio Busacca, Marco Govoni, Leonardo Vivarelli, Nicolandrea Del Piccolo, Alessandra Maso, Cesare Stagni, Giovanni Pignatti
Summary: This study evaluated the clinical and radiological results of using a biomimetic collagen-hydroxyapatite composite biomaterial in hip revision surgery. The results showed that this material can effectively and safely restore acetabular bone loss, and patients demonstrated significant improvement in functional recovery.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell Biology
Alberto Ruffilli, Francesca Barile, Michele Fiore, Marco Manzetti, Giovanni Viroli, Antonio Mazzotti, Marco Govoni, Lucia De Franceschi, Dante Dallari, Cesare Faldini
Summary: This study reviewed the records of 340 patients who received allogenic bone graft during spinal fusion surgery and found that positive intraoperative swab culture results were not predictive of an increased risk of infection and did not influence the surgeon's choices in postoperative management.
CELL AND TISSUE BANKING
(2022)
Article
Multidisciplinary Sciences
Martina Alunni Cardinali, Marco Govoni, Matilde Tschon, Silvia Brogini, Leonardo Vivarelli, Assunta Morresi, Daniele Fioretto, Martina Rocchi, Cesare Stagni, Milena Fini, Dante Dallari
Summary: In this study, a new diagnostic tool for Osteoarthritis (OA) was developed using Brillouin and Raman micro-Spectroscopy (BRamS) and Machine Learning. The degeneration degree of OA-affected patients was analyzed by BRamS to identify mechanical and chemical markers, and the results were compared to histological staining. The diagnostic prediction was optimized using a machine learning algorithm, reducing measurement time and allowing potential in vivo characterization of the articular surface through endoscopic probes during arthroscopy.
SCIENTIFIC REPORTS
(2023)
Article
Endocrinology & Metabolism
Michela Battistelli, Enrico Tassinari, Giovanni Trisolino, Marco Govoni, Gianluca Ruspaggiari, Lucia De Franceschi, Dante Dallari, Debora Burini, Roberta Ramonda, Marta Favero, Francesco Traina, Brunella Grigolo, Eleonora Olivotto
Summary: This study compared the differences in ultrastructure and tissue composition of the hip labrum between healthy individuals and those with femoroacetabular impingement (FAI) and early hip osteoarthritis (OA). The findings suggest that both FAI and OA patients exhibit similar pathological changes in labral tissue, indicating that FAI patients may have a higher susceptibility to develop OA.
CALCIFIED TISSUE INTERNATIONAL
(2023)
Meeting Abstract
Cell & Tissue Engineering
Melania Maglio, Matilde Tschon, Leonardo Vivarelli, Marco Govoni, Martina Rocchi, Alessandro Mazzotta, Enrico Pennello, Carmelo Quinto, Stefania Pagani, Dante Dallari, Milena Fini
TISSUE ENGINEERING PART A
(2022)
Article
Biotechnology & Applied Microbiology
Silvia Zia, Barbara Roda, Chiara Zannini, Francesco Alviano, Laura Bonsi, Marco Govoni, Leonardo Vivarelli, Nicola Fazio, Dante Dallari, Pierluigi Reschiglian, Andrea Zattoni
Summary: Adipose tissue is a widely available source of stem cells. This study investigated the use of the Non-Equilibrium Earth Gravity Assisted Dynamic Fractionation (NEEGA-DF) method to separate cells based on their physical characteristics. The results showed that this method is capable of purifying and analyzing adipose stromal vascular fraction cells, providing a potential tool for medical applications.
BIOENGINEERING-BASEL
(2022)
Article
Multidisciplinary Sciences
M. Alunni Cardinali, A. Di Michele, M. Mattarelli, S. Caponi, M. Govoni, D. Dallari, S. Brogini, F. Masia, P. Borri, W. Langbein, F. Palombo, A. Morresi, D. Fioretto
Summary: This study unveils the potential of Brillouin-Raman microspectroscopy (BRaMS) in assessing the mechanical and chemical properties of bone. The complex microarchitecture and informative spectra of bone were revealed through correlative hyperspectral imaging. Additionally, an innovative method for mechanical properties analysis was proposed, enabling the mapping of different tissue regions involved in various physiological processes.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Meeting Abstract
Biophysics
Martina Alunni Cardinali, Marco Govoni, Dante Dallari, Leonardo Vivarelli, Cesare Stagni, Martina Rocchi, Matilde Tschon, Silvia Brogini, Milena Fini, Maurizio Mattarelli, Silvia Caponi, Daniele Fioretto, Assunta Morresi
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
