Small molecule phosphorescent probes for O2 imaging in 3D tissue models

Monitoring of oxygenation is important for physiological experiments investigating the growth, differentiation and function of individual cells in 3D tissue models. Phosphorescence based O-2 sensing and imaging potentially allow this task; however, current probes do not provide the desired bio-distribution and analytical performance. We present several new cell-penetrating phosphorescent conjugates of a Pt(II)-tetrakis(pentafluorophenyl) porphine (PtPFPP) dye produced by click-modification with thiols, and perform their evaluation as O-2 imaging probes for 3D tissue models. The hydrophilic glucose (Pt-Glc) and galactose (Pt-Gal) conjugates demonstrated minimal aggregation and self-quenching in aqueous media, and efficient in-depth staining of different cell types and multi-cellular aggregates at working concentrations <= 10 mu M. The Pt-Glc probe was applied in high-resolution phosphorescence lifetime based O-2 imaging (PLIM) in multi-cellular spheroids of cancer cells (PC12), primary neural cells (neurospheres) and slices of brain tissue, where it showed good analytical performance, minimal effects on cell viability and appropriate responses to O-2 with phosphorescence lifetimes changing from 20 mu s in air-saturated to 57 mu s under deoxygenated conditions. In contrast, mono-and tetra-substituted oligoarginine conjugates of PtPFPP showed marked aggregation and unstable photophysical properties precluding their use as O-2 sensing probes.