Journal
BIOMATERIALS SCIENCE
Volume 2, Issue 10, Pages 1440-1449Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4bm00202d
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Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Alberta Crop Industry Development Fund Ltd (ACIDF)
- Alberta Livestock and Meat Agency Ltd (ALMA)
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Cellulose-based nanoparticles were prepared from oppositely charged carboxymethyl cellulose (CMC) and quaternized cellulose (QC) in an aqueous medium. The DNA complexing capacity of CMC-QC nanoparticles and the transfection efficiency of the DNA-loaded nanoparticles in COS-7 cells were first investigated using pEGFP-N1 a plasmid DNA encoding the enhanced green fluorescent protein (EGFP) as a model, and then with a candidate DNA vaccine, pMASIA-tPAs-tE2.2, that has been developed against infection with bovine viral diarrhea virus (BVDV). The results revealed that CMC-QC nanoparticles could bind DNA efficiently, and the optimized DNA-loaded nanoparticles mediated very effective transfection in COS-7 cells, which was comparable to that achieved with Lipofectamine 2000. The novel CMC-QC nanoparticles show promise as a delivery system for DNA vaccines.
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