Journal
BIOMATERIALS SCIENCE
Volume 2, Issue 3, Pages 390-398Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3bm60204d
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Funding
- National Natural Science Foundation of China [51033005, 21074121, 21090354, 81171829]
- Provincial Natural Science Foundation of Anhui [KJ2011ZD04]
- Program for New Century Excellent Talents in Universities [NCET-08-0520]
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Disulfide-exchange was found to cross-link the polyplex of disulfide-containing poly(amido amine) and pDNA with heating of the polyplex solution over a short time. The cross-linked polyplexes based on disulfide-containing poly(amido amine) have excellent stability under physiological salt conditions, and have significantly enhanced transfection activity in the serum media compared to non-cross-linked polyplexes. In vivo, ICR mice were injected with the polyplex through the tail vein, the results show that the transfection efficiency of the cross-linked polyplex is higher than that of the non-cross-linked variety. Furthermore, the polyplex containing Cy5 labelled DNA was also injected into the mice to illustrate the stability and distribution of the polyplex, cross-linked polyplexes show a much brighter luminescence than the non-cross-linked ones. This method does not need a cross-linker or catalyst, and there are no impurities produced, it may be an elegant approach to resolve the dilemma of in vivo application of a DNA polyplex, with excellent stability whilst in circulation and a rapid unpacking of the polyplex inside the cells.
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