Journal
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
Volume 43, Issue 1, Pages 50-61Publisher
INFORMA HEALTHCARE
DOI: 10.3109/21691401.2013.837476
Keywords
alginate; bioartificial liver; CYP450 activity; chitosan; cryopreservation; hepatocyte; encapsulation; synthetic metabolism; xenobiotic metabolism
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Funding
- TUBA (The Turkish Academy of Sciences)
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Encapsulation techniques have the potential to protect hepatocytes from cryoinjury. In this study, we comparatively evaluated the viability and metabolic function of primary rat hepatocytes encapsulated in calcium alginate microbeads, in chitosan tripolyphosphate beads, and in three-layered alginate-chitosan-alginate (ACA) microcapsules, before and after cryopreservation at -80 degrees C and in liquid nitrogen (LN2) for 1 and 3 months. Findings demonstrated that LN2 was atop of -80 degrees C in regard to preservation of viability (> 90%) and hepatic functions. LN2-cryopreserved hepatocytes encapsulated in ACA microcapsules retained metabolic function post-thawing, with > 90% of the albumin, total protein and urea syntheses activities, and > 80% of oxidative function.
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