Journal
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
Volume 41, Issue 2, Pages 98-102Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/10731199.2012.712043
Keywords
agitation speed; cord blood; ex vivo expansion; fluid shear stress; hematopoietic stem/progenitor cells; stirred suspension culture
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Funding
- National Natural Science Foundation of China [20776043]
- Key Project of Medicine, Shanghai [074319109]
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Tehe mononuclear cells were cultivated in stirred flasks at different agitation speeds of 30 rpm, 45 rpm, 60 rpm and 80 rpm. At the agitation speed of 30 rpm, total cells achieved higher expansion folds and the CFC density increased. When at higher agitation speed of 60 rpm or 80 rpm, the number of cells dropped rapidly and characteristics of hematopoietic stem/progenitor cells (HSPCs) were not maintained. Moreover, the culture duration of 6-9 days was better for HSPCs ex vivo expansion. These data indicated that HSPCs should be cultured at relatively low agitation speed and for a short-term period when cultured in stirred suspension system.
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