Journal
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
Volume 21, Issue 6, Pages 539-542Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/PAI.0b013e31827d7f16
Keywords
major histocompatibility complex class I and II expression; immunostaining; idiopathic inflammatory myopathy
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Introduction:We sought to study the intensity and pattern of major histocompatibility complex (MHC) I and II expression in muscle from patients with biopsy-proven idiopathic inflammatory myositis (IIM) including the subgroups, polymyositis (PM), dermatomyositis (DM), and inclusion body myositis (IBM).Methods:A total of 120 muscle biopsies (61 PM, 14 DM, and 45 IBM) were immunostained for MHC I and II. Staining was graded as follows. 0: no staining, 1+: 10% fibers, 2+: 10% to 25%, 3+: 25% to 50%, 4+: 50% to 99%, and 5+ 100%.Results:All IIM biopsies showed MHC I positivity; 93% showed MHC II positivity. The proportion of patients with MHC II score 3+ was higher in IBM than DM or PM. In DM, MHC I expression showed a perifascicular pattern. All IBM biopsies were immunopositive for MHC I and II; 30/45 were scored 5+.Discussion:Immunostaining for MHC I and II is a useful adjunctive test in diagnosis and subclassification of IIM.
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