Article
Developmental Biology
Akane Morohoshi, Haruhiko Miyata, Yuki Oyama, Seiya Oura, Taichi Noda, Masahito Ikawa
Summary: The acrosome is a cap-shaped organelle derived from the Golgi apparatus, located over the anterior of the sperm nucleus, and highly conserved throughout evolution. FAM71F1 plays a crucial role in male fertility by forming a complex with active RAB2A/B to suppress excessive vesicle trafficking during acrosome formation.
Article
Cell Biology
Naila Umer, Lena Arevalo, Sharang Phadke, Keerthika Lohanadan, Gregor Kirfel, Dominik Sons, Denise Sofia, Walter Witke, Hubert Schorle
Summary: Profilin (PFN) proteins play crucial roles in actin polymerization and are encoded by four Pfn genes in mice and humans. PFNs, particularly PFN3, are essential for spermiogenesis, sperm morphology, and fertility in males. Lack of PFN3 leads to abnormal acrosome formation, reduced sperm motility, and dysregulation of autophagy, ultimately impacting male reproductive function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Fabiana Faja, Francesco Pallotti, Francesco Cargnelutti, Giulia Senofonte, Tania Carlini, Andrea Lenzi, Francesco Lombardo, Donatella Paoli
Summary: The study showed that DPY19L2 defects are the most common genetic alteration in Italian patients with globozoospermia, while sperm with acrosomal defects may also exhibit high levels of sperm DNA fragmentation.
Article
Obstetrics & Gynecology
Lars Bjorndahl, Jackson Kirkman Brown
Summary: The principles of basic semen investigation are crucial for obtaining reliable and internationally comparable results. However, many studies and practices in reproductive medicine fail to adhere to these principles, leading to a lack of comparable data and accuracy. To address this issue, the sixth edition of the WHO manual introduces revised methods and a complementary formal standard from the International Standards Organization. The new edition also reintroduces the four-category distinction of sperm motility and emphasizes the importance of semen examination for assessing male reproductive functions and overall male health.
FERTILITY AND STERILITY
(2022)
Article
Biochemistry & Molecular Biology
Ya-Lan Wei, Xiao-Jing Fan, Xin-Chen Lin, Ai-Zhu Lin, Zhen-Yu She, Xin-Rui Wang
Summary: In this study, we revealed the essential role of KIFC1 in regulating the trafficking, fusion, and maturation of acrosomal vesicles during spermiogenesis. Inhibition of KIFC1 resulted in structural deformities of acrosomes and multinucleated undifferentiated spermatogenic cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Anatomy & Morphology
Maider Bizkarguenaga, Laura Gomez-Santos, Juan Francisco Madrid, Francisco Jose Saez, Edurne Alonso
Summary: The study found that improper spermiogenesis leads to deregulation of ZP3R expression in globozoospermic spermatids, but even though the lack of GOPC causes failures in pre-acrosomal vesicle transport, the ZP3R protein still localizes in the acrosome and is only distributed through the cytoplasm during spermiogenesis.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Editorial Material
Cell Biology
Hana Popelka, Daniel J. J. Klionsky
Summary: A recent study used cryo-electron tomography combined with computational analysis to gain insights into autophagosome biogenesis in yeast cells. This approach provided new information on autophagic structures, their contacts with organelles, membrane sources, and transition mechanisms. These findings open new avenues for autophagy research and highlight the potential of cryo-ET in cell biology.
Article
Andrology
Yoshitaka Fujihara, Kiyonori Kobayashi, Ferheen Abbasi, Tsutomu Endo, Zhifeng Yu, Masahito Ikawa, Martin M. Matzuk
Summary: In this study, Pdcl2 knockout mice were generated to investigate the essential roles of PDCL2 in mammalian reproduction. The findings showed that PDCL2 is crucial for sperm acrosome development and male fertility, suggesting it as a potential contraceptive target in men.
Article
Obstetrics & Gynecology
Pingping Chen, Hexige Saiyin, Ruona Shi, Bin Liu, Xu Han, Yuping Gao, Xiantao Ye, Xiaofei Zhang, Yu Sun
Summary: The study identified a critical role of the SPACAI gene in globozoospermia, expanding the spectrum of causative genes. Additionally, evidence was provided for ICSI clinical outcomes in patients with SPACAI deficiency, potentially guiding treatment strategies. The study also explored the pathogenesis of globozoospermia caused by SPACAI deficiency.
HUMAN REPRODUCTION
(2021)
Article
Developmental Biology
Gabriela Carrasquel Martinez, Andres Aldana, Jorge Carneiro, Claudia Lydia Trevino, Alberto Darszon
Summary: Mammalian sperm capacitation, involving biochemical and physiological modifications, is a crucial step for successful fertilization. This study showed that the acrosome pH gradually increases during human sperm capacitation, and the V-ATPase plays a key role in regulating the acrosome acidity. These findings provide important insights into the factors influencing sperm fusion and fertilization.
MOLECULAR HUMAN REPRODUCTION
(2022)
Review
Reproductive Biology
Wenfeng Xiong, Chunling Shen, Zhugang Wang
Summary: Sexual reproduction involves the fusion of two gametes through fertilization, with acrosome reaction playing a crucial role in ensuring successful fertilization. Acrosome, a specialized organelle covering the anterior portion of the sperm head, undergoes a multistep biogenesis process requiring coordination between various organelles and cytoplasmic structures. Research on acrosome formation in gene-manipulated mice has revealed a complex molecular regulatory mechanism involving multiple interacting proteins.
BIOLOGY OF REPRODUCTION
(2021)
Article
Chemistry, Medicinal
Yukiko Karuo, Riona Shiraki, Ayaka Yoshida, Ryo Tsunokawa, Mayuko Nakahara-Yamada, Atsushi Tarui, Kazuyuki Sato, Kentaro Kawai, Masaaki Omote, Hitoshi Nishimura
Summary: By investigating sperm activation mechanisms in Caenorhabditis elegans and mice, a compound named DDI-6 was found to moderately activate both types of sperm.
CHEMICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Reproductive Biology
Camilo Hernandez-Aviles, Luisa Ramirez-Agamez, Dickson D. Varner, Charles C. Love
Summary: The process of fertilization involves acrosomal exocytosis (AE) as the interaction between sperm and oocyte. While the role of sperm acrosome in fertilization has been extensively studied, there is limited knowledge about the molecular mechanisms governing acrosomal function in non-mice or human species. Evaluating sperm acrosomal function should be a routine test for individuals selected for breeding potential, even though acrosomal dysfunction-related subfertility is less common in large animals. High throughput technologies such as flow cytometry and mass spectrometry-based proteomic analysis can be used to study acrosomal function and discover new markers of acrosomal dysfunction in stallions.
Article
Biochemistry & Molecular Biology
Roland Abi Nahed, Magali Dhellemmes, Christine Payre, Emilie Le Blevec, Jean-Philippe Perrier, Sylviane Hennebicq, Jessica Escoffier, Pierre F. Ray, Corinne Loeuillet, Gerard Lambeau, Christophe Arnoult
Summary: The secreted phospholipase A2 (PLA2G10) plays an important role in mouse fertilization by enhancing acrosome reaction and secretion, improving fertilization rate, and binding to PLA2R1 on gametes. It is shown that the catalytic activity and PLA2R1 binding of PLA2G10 both contribute to its profertility effect, and the study provides new insights into the complexity of PLA2G10's action in optimizing fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shuai Wang, Hongjie Qiao, Pengxiang Wang, Yuan Wang, Danian Qin
Summary: Zdhhc19 knockout mice did not show disruption in spermatogenesis but exhibited male infertility with defects in sperm morphology and functions, highlighting the essential role of Zdhhc19 in sperm production and fertility in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Andrology
Jessika Moreau, Nicolas Gatimel, Jean Parinaud, Roger Leandri
ASIAN JOURNAL OF ANDROLOGY
(2018)
Article
Endocrinology & Metabolism
Jessika Moreau, Nicolas Gatimel, Clementine Cohade, Jean Parinaud, Roger Leandri
GYNECOLOGICAL ENDOCRINOLOGY
(2018)
Article
Obstetrics & Gynecology
Clelia Chalumeau, Jessika Moreau, Nicolas Gatimel, Clementine Cohade, Florence Lesourd, Jean Parinaud, Roger Leandri
REPRODUCTIVE BIOMEDICINE ONLINE
(2018)
Article
Endocrinology & Metabolism
Cynthia Simon, Jessika Moreau, Nicolas Gatimel, Clementine Cohade, Jean Parinaud, Roger Leandri
GYNECOLOGICAL ENDOCRINOLOGY
(2019)
Article
Obstetrics & Gynecology
Cynthia Simon, Lise Branet, Jessika Moreau, Nicolas Gatimel, Clementine Cohade, Florence Lesourd, Jean Parinaud, Roger Leandri
REPRODUCTIVE BIOMEDICINE ONLINE
(2019)
Article
Andrology
J. Moreau, S. Fargeon, N. Gatimel, J. Parinaud, R. D. Leandri
Article
Obstetrics & Gynecology
Nadia Prisant, Guillaume Joguet, Catherine Moriniere, Lynda Pavili, Sylvia Lurel, Cecile Herrmann-Stock, Louis Bujan
REPRODUCTIVE BIOMEDICINE ONLINE
(2019)
Article
Andrology
S. El Fekih, C. Tous, N. Gueganic, F. Brugnon, H. Ben Ali, L. Bujan, N. Moinard, E. Caire-Tetauru, M. Ajina, N. Douet-Guilbert, F. Morel, A. Perrin
Article
Environmental Sciences
Veronique Gavrard, Marlene Z. Lacroix, Flare C. Grandin, Severine H. Collet, Hanna Mila, Catherine Viguie, Clemence A. Gely, Blandine Rabozzi, Michele Bouchard, Roger Leandri, Pierre-Louis Toutain, Nicole Picard-Hagen
ENVIRONMENTAL HEALTH PERSPECTIVES
(2019)
Article
Reproductive Biology
Mohamed Hadi Mohamed Abdelhamid, Camille Esquerre-Lamare, Marie Walschaerts, Gulfam Ahmad, Roger Mieusset, Safouane Hamdi, Louis Bujan
REPRODUCTIVE BIOLOGY
(2019)
Review
Genetics & Heredity
Nicolas Gatimel, Jessika Moreau, Jean Parinaud, Roger D. Leandri
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2020)
Article
Pharmacology & Pharmacy
Veronique Gayrard, Marlene Z. Lacroix, Clemence A. Gely, Flore C. Grandin, Roger Leandri, Michele Bouchard, Beatrice Roques, Pierre-Louis Toutain, Nicole Picard-Hagen
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2020)
Article
Obstetrics & Gynecology
J. Moreau, N. Gatimel, Y. Lippi, G. Tavenier, P. Fauque, M. Guilleman, C. Naylies, A. A. Huesca, V Gayrard, J. Parinaud, R. D. Leandri
Summary: An experimental study using mouse embryos found that daily manipulations with PC-made strippers enhanced blastocyst development rate compared to glass strippers, but this effect was not BPA-related. No differential gene expression, including epigenetic markers, was observed in embryos manipulated with PC strippers, suggesting that the use of BPA in plastics near human embryos warrants further investigation.
HUMAN REPRODUCTION
(2021)
Article
Environmental Sciences
Sara El Fouikar, Veronique Duranthon, Virginie Helies, Helene Jammes, Anne Couturier-Tarrade, Veronique Gayrard, Nathalie Van Acker, Francois-Xavier Frenois, Catherine Archilla, Delphine Rousseau-Ralliard, Nicolas Gatimel, Roger Leandri
Summary: Animal toxicological studies often fail to mimic the complexity of the human exposome, making it difficult to understand the reproductive health effects of environmental toxicants. The FEDEXPO project aims to evaluate the impacts of exposure to a mixture of endocrine disrupting chemicals (EDCs) on ovarian function and reproductive health in a rabbit model. The project will also monitor the development and health of offspring from the preimplantation stage, with a focus on reproductive health. Additionally, the study will explore potential mechanisms for the inheritance of health disruption via the oocyte or the preimplantation embryo.
Letter
Andrology
J. Moreau, N. Gatimel, J. Parinaud, R. D. Leandri