4.7 Article

Rapid triacylglyceride detection and quantification in live micro-algal cultures via liquid state 1H NMR

Journal

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
Volume 1, Issue 2, Pages 166-175

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.algal.2012.07.003

Keywords

Microalgae; NMR; Triacylglycerides; Biofuels; Lipid

Funding

  1. NIH [RR0631401, RR12948]
  2. NSF [CHE-9115282, DBI-9604689]
  3. Murdock Charitable Trust
  4. DOE [DE-EE0003112]

Ask authors/readers for more resources

Non-invasive methods for measuring lipid content in live microalgal cultures are critically needed for algal biofuel research and development. A non-destructive method requiring minimal sample preparation was developed utilizing liquid state H-1 NMR for quantifying triacylglycerides (TAGS) in live algae cultures. H-1 NMR and H-1 HR-MAS spectra of live algae cells show outstanding correlation with published chemical shifts for TAGs. H-1 NMR spectra of Chlamydomonas reinhardtii whole cells and isolated lipid bodies were compared with a standard oleic TAG H-1 spectrum, demonstrating that only lipid body TAGs were observed. A model TAG was derived, providing a proton count and molecular weight for conversion of TAG H-1 NMR integrals to volumetric TAG or fatty acid methyl ester (FAME) equivalent concentrations, which were correlated to FAME concentrations by gas chromatography (FAME-GC) at several time points. A customized NMR flow cell was subsequently constructed, allowing real-time, continuous measurements of multiple cultures. (c) 2012 Elsevier B.V. All rights reserved.

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