Molecular Approaches to Improve the Insecticidal Activity of Bacillus thuringiensis Cry Toxins

A conserved tetrameric interaction of cry toxin helix α3 suggests a functional role for toxin oligomerization

(2014) Xin Lin et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

New Insight to Structure-Function Relationship of GalNAc Mediated Primary Interaction between Insecticidal Cry1Ac Toxin and HaALP Receptor of Helicoverpa armigera

(2013) Anindita Sengupta et al.   PLoS One

Structure and Glycolipid Binding Properties of the Nematicidal Protein Cry5B

(2012) Fan Hui et al.   BIOCHEMISTRY

Current models of the mode of action of Bacillus thuringiensis insecticidal crystal proteins: A critical review

(2012) Vincent Vachon et al.   JOURNAL OF INVERTEBRATE PATHOLOGY

Affinity Maturation of Cry1Aa Toxin to the Bombyx mori Cadherin-Like Receptor by Directed Evolution

(2012) Yuki Fujii et al.   MOLECULAR BIOTECHNOLOGY

Evolution ofBacillus thuringiensisCry toxins insecticidal activity

(2012) Alejandra Bravo et al.   Microbial Biotechnology

Molecular cloning, characterization, genomic organization and promoter analysis of the α1,6-fucosyltransferase gene (fut8) expressed in the rat hybridoma cell line YB2/0

(2011) Béatrice Teylaert et al.   BMC BIOTECHNOLOGY

Bacillus thuringiensis: A story of a successful bioinsecticide

(2011) Alejandra Bravo et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Mortality Impact of Bt Transgenic Maize Roots Expressing eCry3.1Ab, mCry3A, and eCry3.1Ab Plus mCry3A on Western Corn Rootworm Larvae in the Field

(2011) Bruce E. Hibbard et al.   JOURNAL OF ECONOMIC ENTOMOLOGY

Participation of Valine 171 in  -Helix 5 of Bacillus thuringiensis Cry1Ab  -Endotoxin in Translocation of Toxin into Lymantria dispar Midgut Membranes

(2010) O. Alzate et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Lepidopteran-Active Variable-Region Sequence Imparts Coleopteran Activity in eCry3.1Ab, an Engineered Bacillus thuringiensis Hybrid Insecticidal Protein

(2010) F. S. Walters et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A Cry1Ac Toxin Variant Generated by Directed Evolution has Enhanced Toxicity against Lepidopteran Insects

(2010) Shiping Shan et al.   CURRENT MICROBIOLOGY

The Role of β20–β21 Loop Structure in Insecticidal Activity of Cry1Ac Toxin from Bacillus thuringiensis

(2010) Yuan Lv et al.   CURRENT MICROBIOLOGY

Role of Alkaline Phosphatase fromManduca sextain the Mechanism of Action ofBacillus thuringiensisCry1Ab Toxin

(2010) Iván Arenas et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Short-Term Evaluation in Growing Rats of Diet ContainingBacillus thuringiensisCry1Ia12 Entomotoxin: Nutritional Responses and Some Safety Aspects

(2010) Luciane Mourão Guimarães et al.   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

Characterization of a Cry1Ac toxin-binding alkaline phosphatase in the midgut from Helicoverpa armigera (Hübner) larvae

(2010) Changming Ning et al.   JOURNAL OF INSECT PHYSIOLOGY

Analysis of Cry8Ka5-binding proteins from Anthonomus grandis (Coleoptera: Curculionidae) midgut

(2010) Erich Y.T. Nakasu et al.   JOURNAL OF INVERTEBRATE PATHOLOGY

Residue 544 in Domain III of the Bacillus thuringiensis Cry1Ac Toxin is Involved in Protein Structure Stability

(2010) Yong Le Liu et al.   PROTEIN JOURNAL

Signaling versus punching hole: How do Bacillus thuringiensis toxins kill insect midgut cells?

(2009) M. Soberón et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Domain II Loop 3 ofBacillus thuringiensisCry1Ab Toxin Is Involved in a “Ping Pong” Binding Mechanism with Manduca sexta Aminopeptidase-N and Cadherin Receptors

(2009) Sabino Pacheco et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Variant Cry1Ia toxins generated by DNA shuffling are active against sugarcane giant borer

(2009) Kilvia I.C. Craveiro et al.   JOURNAL OF BIOTECHNOLOGY

N546 in β18–β19 loop is important for binding and toxicity of the Bacillus thuringiensis Cry1Ac toxin

(2009) Wang Fa Xiang et al.   JOURNAL OF INVERTEBRATE PATHOLOGY

Crystal structure of Bacillus thuringiensis Cry8Ea1: An insecticidal toxin toxic to underground pests, the larvae of Holotrichia parallela

(2009) Shuyuan Guo et al.   JOURNAL OF STRUCTURAL BIOLOGY

Dominant Negative Mutants of Bacillus thuringiensis Cry1Ab Toxin Function as Anti-Toxins: Demonstration of the Role of Oligomerization in Toxicity

(2009) Claudia Rodríguez-Almazán et al.   PLoS One

Helix  4 of the Bacillus thuringiensis Cry1Aa Toxin Plays a Critical Role in the Postbinding Steps of Pore Formation

(2008) F. Girard et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Cysteine Scanning Mutagenesis of  4, a Putative Pore-Lining Helix of the Bacillus thuringiensis Insecticidal Toxin Cry1Aa

(2008) F. Girard et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Investigating the Properties of Bacillus thuringiensis Cry Proteins with Novel Loop Replacements Created Using Combinatorial Molecular Biology

(2008) C. R. Pigott et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Location of the Bombyx mori 175 kDa cadherin-like protein-binding site on Bacillus thuringiensis Cry1Aa toxin

(2008) Shogo Atsumi et al.   FEBS Journal

The pre-pore from Bacillus thuringiensis Cry1Ab toxin is necessary to induce insect death in Manduca sexta

(2008) N. Jiménez-Juárez et al.   PEPTIDES

Strategies to improve the insecticidal activity of Cry toxins from Bacillus thuringiensis

(2008) L. Pardo-López et al.   PEPTIDES

How to cope with insect resistance to Bt toxins?

(2008) Alejandra Bravo et al.   TRENDS IN BIOTECHNOLOGY