Article
Cell & Tissue Engineering
Hao Liu, Tianyue Pan, Yifan Liu, Yuan Fang, Gang Fang, Xiaolang Jiang, Bin Chen, Zheng Wei, Shiyang Gu, Peng Liu, Weiguo Fu, Zhihui Dong
Summary: This study reports the 5-year outcomes of a randomized single-blinded noninferiority trial on peripheral blood mononuclear cells (PBMNCs) and purified CD34(+) cells (PCCs) transplantation for treating angiitis-induced critical limb ischemia (AICLI). The results showed similar efficacy and safety for the two types of autoimplants and a satisfactory cumulative return to work (RTW) rate in AICLI patients who underwent cell transplantation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Hao Liu, Yuan Fang, Tianyue Pan, Gang Fang, Yifan Liu, Xiaolang Jiang, Bin Chen, Shiyang Gu, Zheng Wei, Peng Liu, Weiguo Fu, Jue Yang, Zhihui Dong
Summary: This study investigated the recurrence conditions of a relatively large cohort of patients with angiitis-induced critical limb ischemia (AICLI). The results showed that patients with AICLI who underwent cell transplantation had a higher risk of recurrence if multiple limbs were involved and if the period of ischemia relief was longer.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Urology & Nephrology
Mu-Yang Hsieh, Shao-Yuan Chuang, Chih-Kuo Lee, Chien-Ming Luo, Chi-Hung Cheng, Min-Tsun Liao, Po-Lin Lin, Ten-Fang Yang, Chih-Cheng Wu
Summary: This study evaluated the incidence, risk factors, and outcomes of critical limb ischemia (CLI) in patients undergoing hemodialysis. It found that patients on hemodialysis had a higher rate of newly diagnosed PAD, and those with CLI had a higher risk for amputation or PAD-related death. Clinical variables such as disability, diabetes mellitus, current smoking, and atrial fibrillation were significantly associated with the development of newly diagnosed CLI.
CLINICAL KIDNEY JOURNAL
(2023)
Review
Cell & Tissue Engineering
Laura Lozano Navarro, Xueyi Chen, Lady Tatiana Girata Viviescas, Andrea K. Ardila-Roa, Maria L. Luna-Gonzalez, Claudia L. Sossa, Martha L. Arango-Rodriguez
Summary: Peripheral arterial disease affects hundreds of millions of people worldwide and can lead to limb amputation or death. Revascularization is crucial for preventing amputation, but a significant portion of patients are not suitable for traditional surgical treatment, resulting in higher amputation rates. Mesenchymal stem cells have emerged as a promising cell-based therapy for peripheral arterial disease due to their regenerative properties.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Medicine, General & Internal
Alessia Scatena, Pasquale Petruzzi, Filippo Maioli, Francesca Lucaroni, Cristina Ambrosone, Giorgio Ventoruzzo, Francesco Liistro, Danilo Tacconi, Marianna Di Filippi, Nico Attempati, Leonardo Palombi, Leonardo Ercolini, Leonardo Bolognese
Summary: The study showed that treatment with autologous PBMNC implants in NO-CLI patients with DFUs significantly reduced amputation rate, improved survival, and promoted wound healing. Autologous PBMNC injection may effectively prevent amputations in patients with DFUs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell Biology
Yipeng Du, Xiaoting Li, Wenying Yan, Zhaohua Zeng, Dunzheng Han, Hong Ouyang, Xiudi Pan, Bihui Luo, Bohua Zhou, Qiang Fu, Dongfeng Lu, Zheng Huang, Zhiliang Li
Summary: The study utilized mutant MetRS to label proteomes of mesenchymal stem cells and demonstrated efficient incorporation of ANL in protein synthesis in ischemic hind limb. The transplanted MSCs significantly improved blood reperfusion and vessel density while reducing inflammation in hindlimb ischemia model. Proteomic analysis revealed differential protein expression in apoptosis and energy metabolism pathways between ischemic and sham groups.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Medicine, General & Internal
Bobak Moazzami, Zinat Mohammadpour, Zohyra E. Zabala, Ermia Farokhi, Aria Roohi, Elena Dolmatova, Kasra Moazzami
Summary: This study aimed to assess the benefits and harms of local intramuscular transplantation of autologous adult bone marrow mononuclear cells (BMMNCs) as a treatment for critical limb ischaemia (CLI). The results showed that there is currently no clear evidence for the effectiveness of BMMNCs compared to the control group in the treatment of CLI. Larger randomized controlled trials are needed to validate the efficacy of this procedure.
COCHRANE DATABASE OF SYSTEMATIC REVIEWS
(2022)
Review
Cell Biology
Mehdi Hassanpour, Amankeldi A. Salybekov, Shuzo Kobayashi, Takayuki Asahara
Summary: CD34 is a cell surface antigen expressed in stem/progenitor cells such as HSCs and EPCs, which are potential sources for regenerative therapy. CD34(+) cells have been shown to improve therapeutic angiogenesis through direct incorporation and paracrine activity. Preclinical, pilot, and clinical trials have demonstrated the safety and efficacy of CD34(+) cell therapy in various diseases. However, the clinical application of CD34(+) cell therapy has sparked debates and controversies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Pegah Nammian, Seyedeh-Leili Asadi-Yousefabad, Sajad Daneshi, Mohammad Hasan Sheikhha, Seyed Mohammad Bagher Tabei, Vahid Razban
Summary: The study compared the effects of allogeneic bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue mesenchymal stem cells (AT-MSCs) in the treatment of critical limb ischemia (CLI). The results indicated that BM-MSCs had a stronger effect on endothelial cell migration, muscle restructure, functional improvements, and neovascularization in ischemic tissues compared to AT-MSCs. This suggests that BM-MSC transplantation could be a more effective therapy for diseases with insufficient angiogenesis, such as hindlimb ischemia.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Margarita Jimenez-Palomares, Ismael Sanchez-Gomar, Antonio Rosal-Vela, Marta Rojas-Torres, Sara Eslava-Alcon, Jose Angel Alonso-Pinero, Almudena Gonzalez-Rovira, Ma Jesus Extremera-Garcia, Rosario Conejero, Esther Doiz, Manuel Rodriguez-Pinero, Martin R. Larsen, Ma Carmen Duran-Ruiz
Summary: The study suggests that circulating angiogenic cells (CACs) have regenerative potential and can enhance blood flow recovery in mice with critical limb ischemia. Pre-stimulation of CACs with factors secreted by atherosclerotic plaques enhances their regenerative properties. Proteomics analysis identified potential proteins involved in the response to ischemia and the effect of CACs administration, indicating a possible mechanism for the therapeutic effects of CACs in CLI.
Review
Cell & Tissue Engineering
Zeinab Shirbaghaee, Mohammad Hassani, Saeed Heidari Keshel, Masoud Soleimani
Summary: This article reviews the immunomodulatory features of mesenchymal stem cells (MSCs) and their potential applications as therapeutic agents in the treatment of critical limb ischemia (CLI). Despite advances in clinical research, MSC therapy is still considered experimental and not recommended for regular clinical use. The article provides recommendations to improve the efficacy of cell therapy for CLI patients.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Engineering, Biomedical
Zheng Xing, Chen Zhao, Siwen Wu, Chunchen Zhang, Haifeng Liu, Yubo Fan
Summary: Therapeutic angiogenesis provides a potential solution for critical limb ischemia by manipulating blood vessel growth in vivo without the need for extra pharmaceutics and surgery. With the rise of interdisciplinary research, regenerative medicine strategies offer new possibilities for treating various clinical diseases.
Article
Cardiac & Cardiovascular Systems
Xiaolang Jiang, Hao Liu, Tianyue Pan, Shiyang Gu, Yuan Fang, Zheng Wei, Gang Fang, Bin Chen, Junhao Jiang, Yun Shi, Peng Liu, Weiguo Fu, Zhihui Dong
Summary: The study demonstrated that using peripheral blood mononuclear cells for AICLTI treatment has favorable long-term outcomes, improving patients' quality of life, restoring labor competence, and significantly reducing major amputation rates.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cell Biology
Daniel Santillan-Cortez, Eduardo Vera-Gomez, Alejandro Hernandez-Patricio, Atzin Sua Ruiz-Hernandez, Juan Ariel Gutierrez-Buendia, Karen De la Vega-Moreno, Yasser Alberto Rizo-Garcia, Oscar Antonio Loman-Zuniga, Ignacio Escotto-Sanchez, Juan Miguel Rodriguez-Trejo, Mario Antonio Tellez-Gonzalez, Christian Gabriel Toledo-Lozano, Tania Ortega-Rosas, Silvia Garcia, Paul Mondragon-Teran, Juan Antonio Suarez-Cuenca
Summary: This study investigated the relationship between endothelial progenitor cells (EPCs) and outcomes in patients with critical limb ischemia (CLI) undergoing angioplasty. The results showed that EPCs were related to endothelial dysfunction, and a higher baseline number of CD45(+)CD34(+)KDR(+) subset, as well as an impaired reactivity of CD45(+)CD34(+)CD133(+)CD184(+) subset after angioplasty, were predictive for major limb amputation in CLI patients.
Article
Surgery
Robert Myers, Bakhtawar Mushtaq, Nicholas Taylor, Hajar Rashid, Danielle M. Pineda
Summary: This study aims to understand the impact of bypass surgery on limb salvage in patients aged >80 years with critical limb ischemia. The study found that there was no significant difference in limb salvage, hospital length of stay, and primary patency between patients aged <80 years and those aged >=80 years. However, further studies are needed to determine the statistical impact on mortality in this population with a larger cohort.
JOURNAL OF VASCULAR SURGERY
(2023)