Article
Oncology
Kanakaraju Manupati, Ritama Paul, Mingang Hao, Michael Haas, Zhaoqun Christine Bian, Tammy M. Holm, Jun-Lin Guan, Syn Kok Yeo
Summary: Breast cancer stem cells (BCSCs) are a small population of tumor cells with high metastatic potential. The molecule biglycan (BGN) has been identified as a potential molecular target in BCSCs that regulates their aggressive characteristics. Removing BGN reduces BCSC proportions and tumorsphere formation, as well as decreases BCSC-associated migration, invasion, and metabolism. These findings suggest that targeting BGN could be an important therapeutic strategy for treating metastatic breast cancer.
Article
Multidisciplinary Sciences
Chloe Maurizy, Claire Abeza, Benedicte Lemmers, Monica Gabola, Ciro Longobardi, Valerie Pinet, Marina Ferrand, Conception Paul, Julie Bremond, Francina Langa, Francois Gerbe, Philippe Jay, Celine Verheggen, Nicola Tinari, Dominique Helmlinger, Rossano Lattanzio, Edouard Bertrand, Michael Hahne, Berengere Pradet-Balade
Summary: The R2TP chaperone plays essential roles in intestinal cell proliferation and colorectal cancer, with Rpap3 deletion inhibiting cell proliferation in mice intestine, while elevated RPAP3 levels in colorectal tumors are associated with poor prognosis.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Jose D. D. Puentes-Pardo, Sara Moreno-SanJuan, Jorge Casado, Julia Escudero-Feliu, David Lopez-Perez, Paula Sanchez-Uceta, Paula Gonzalez-Novoa, Julio Galvez, Angel Carazo, Josefa Leon
Summary: Poly(ADP-ribose) polymerase-1 (PARP-1) is involved in various physiological processes. Elevated PARP-1 expression is found in several tumors, associated with stemness and tumorigenesis. In colorectal cancer patients, PARP-1 expression correlated with differentiation grade, with a significant association only in tumors with wild-type p53. Additionally, PARP-1 and cancer stem cell markers were positively correlated in these tumors. In vitro studies showed that PARP-1 regulates cancer stem cell phenotype depending on p53 status, with overexpression increasing marker expression and sphere forming ability in wild-type p53 cells, while reducing these features in mutated p53 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Qiqi Tang, Beibei Liang, Lisha Zhang, Xuhui Li, Hengyu Li, Wei Jing, Yingjie Jiang, Felix Zhou, Jian Zhang, Yanchun Meng, Xinhua Yang, Hao Yang, Gang Huang, Jian Zhao
Summary: Our study reveals a novel mechanism for de novo cholesterol biosynthesis in regulating CCDC25 expression, NETs formation, and breast cancer metastasis. Targeting cholesterol biosynthesis may be a promising therapeutic strategy for treating breast cancer metastasis.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Shao Wenhua, Takaaki Tsunematsu, Masaaki Umeda, Hiroaki Tawara, Natsumi Fujiwara, Yasuhiro Mouri, Rieko Arakaki, Naozumi Ishimaru, Yasusei Kudo
Summary: Recently, it has been reported that the partial-epithelial-mesenchymal transition (p-EMT) program is associated with metastasis in head and neck squamous cell carcinoma (HNSCC). The study focused on the role of a novel cancer-specific POSTN isoform, Iso5, in HNSCC and found that Iso5 promoted invasion together with another isoform, Iso3. The researchers suggest that Iso5 can be a useful marker for detecting cancer cells undergoing p-EMT and a potential target for diagnosis and therapy in HNSCC.
Article
Biochemistry & Molecular Biology
Libang Yang, Adam Gilbertsen, Blake Jacobson, Jenny Pham, Naomi Fujioka, Craig A. Henke, Robert A. Kratzke
Summary: Cancer markers are measurable molecules produced by tumor cells or immune cells, and they play a crucial role in clinical diagnosis, prognosis, and anti-drug monitoring. SFPQ, a protein, shows significant differences in its expression between normal and cancer cells, and its elevated levels and isoform location could serve as a diagnostic and prognostic marker for lung cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Hongbin Su, Mengdi Qin, Qiang Liu, Bo Jin, Xianjun Shi, Zheng Xiang
Summary: UBD, a member of the UBL family, is highly expressed in CRC and plays a regulatory role in promoting CRC cell proliferation. Overexpression of UBD in CRC tissues is associated with tumor size and TNM stage of patients. Mechanistically, UBD interacts with p53, leading to its degradation and subsequent effects on cell cycle regulators, ultimately promoting CRC progression.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kuo-Chu Lai, Prabha Regmi, Chung-Ji Liu, Jeng-Fan Lo, Te-Chang Lee
Summary: The study demonstrates that IFIT2 depletion promotes the CSC-like phenotypes in oral cancer cells. Clinically, there is a positive correlation between IFIT2 and CD24 in oral cancer patients, and the expression pattern of IFIT2(high)/CD24(high)/CD44(low) predicts a better prognosis. Furthermore, TNF-alpha blockade can abolish the sphere formation induced by IFIT2 depletion, suggesting its involvement in CSC-like phenotypes in oral cancer.
Article
Oncology
Taoyuan Wang, Tiansheng Tang, Youguo Jiang, Tao He, Luyu Qi, Hongkai Chang, Yaya Qiao, Mingming Sun, Changliang Shan, Xinyuan Zhu, Jianshi Liu, Jiyan Wang
Summary: p53 mutations are common in human cancer, and in this study, it was found that elevated expression of PRIM2 in p53-mutated lung cancer is associated with tumor progression. The expression of PRIM2 is regulated by p53 and serves as a biomarker for lung cancer malignancy and survival prognosis.
Article
Oncology
Jie Jiang, Li Gao, Yongting Lan, Yang Wang, Peiqing Zhao
Summary: Previous studies have shown that TIPE1 acts as a tumor suppressor gene in several tumor types, but in cervical cancer, it promotes proliferation by inhibiting p53 activity, suppressing cell apoptosis, and facilitating chemoresistance in a p53-dependent manner. This suggests that TIPE1 plays a crucial role in the transition from chemosensitivity to chemoresistance and could be a potential target for cervical cancer chemotherapy.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ashutosh S. Yende, Emily C. Williams, Andrew Pletcher, Alexandra Helfand, Helen Ibeawuchi, Tanya M. North, Patricia S. Latham, Anelia Horvath, Maho Shibata
Summary: TRIM28 is found to be significantly upregulated in high-grade and metastatic prostate cancers. Through a genetically-engineered mouse model, scientists discovered that TRIM28 plays an important role in the progression of prostate cancer. Despite a reduction in luminal cells resembling proximal luminal lineage cells, the mouse prostates still evolved into invasive prostate carcinoma with a shorter overall survival.
Article
Hematology
Sofia Gialesaki, Daniela Braeuer-Hartmann, Hasan Issa, Raj Bhayadia, Oriol Alejo-Valle, Lonneke Verboon, Anna-Lena Schmell, Stephanie Laszig, Eniko Regenyi, Konstantin Schuschel, Maurice Labuhn, Michelle Ng, Robert Winkler, Christian Ihling, Andrea Sinz, Markus Glass, Stefan Huettelmaier, Sören Matzk, Lena Schmid, Farina Josepha Struewe, Sofie-Katrin Kadel, Dirk Reinhardt, Marie-Laure Yaspo, Dirk Heckl, Jan-Henning Klusmann
Summary: It is proposed that the imbalance of the RUNX1 isoform, with a bias towards RUNX1A, is key to the development of myeloid leukemia in individuals with Down syndrome. Excess RUNX1A in combination with the Gata1s mutation displaces RUNX1C and induces oncogenic programs with the MYC cofactor MAX, an effect that can be reversed by restoring the balance between RUNX1A and RUNX1C. Additionally, targeting MYC:MAX dimerization shows strong anti-leukemic effects.
Article
Biochemistry & Molecular Biology
Ravi Gor, Shruthi Sanjitha Sampath, Lizha Mary Lazer, Satish Ramalingam
Summary: This study is the first to demonstrate the role of PUM1 in colon cancer development, showing its high expression in colon cancer cells and its promotion of proliferation, migration, and colony formation. PUM1 overexpression also leads to an increase in spheroid size and number, indicating its involvement in maintaining cancer stem cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Multidisciplinary Sciences
Vivek K. Dwivedi, Carlos Pardo-Pastor, Rita Droste, Ji Na Kong, Nolan Tucker, Daniel P. Denning, Jody Rosenblatt, H. Robert Horvitz
Summary: Cell extrusion is a mechanism of cell elimination utilized by various organisms. Through a genome-wide RNA interference screen, S-phase specific cell cycle genes were identified to play a role in the extrusion process. Cells experiencing replication stress in S-phase activate a replication-stress response, leading to cell extrusion. This mechanism is conserved across different species.
Review
Biochemistry & Molecular Biology
Sunali Mehta, Hamish Campbell, Catherine J. Drummond, Kunyu Li, Kaisha Murray, Tania Slatter, Jean-Christophe Bourdon, Antony W. Braithwaite
Summary: p53 is a crucial protein in maintaining biological homeostasis, with its network playing a vital role in maintaining stability within multicellular organisms, facilitating cooperation between cells to adapt to environmental changes.
Article
Anesthesiology
J. A. Dubowitz, J. P. Cata, A. P. De Silva, S. Braat, D. Shan, K. Yee, F. Hollande, O. Martin, E. K. Sloan, B. Riedel
Summary: The study explored the feasibility of conducting a randomised controlled trial to investigate the impact of total intravenous anaesthesia with propofol versus inhalational volatile anaesthesia on postoperative outcomes for cancer patients. The successful recruitment rate and adherence to assigned anaesthetic treatment were high, and intra-operative and postoperative complications were comparable between the two intervention groups. This feasibility and pilot study supports the viability of a future large, randomised controlled trial to investigate the effect of anaesthesia technique on postoperative cancer outcomes.
Article
Medicine, Research & Experimental
Sophia Ceder, Sofi E. Eriksson, Emarndeena H. Cheteh, Swati Dawar, Mariana Corrales Benitez, Vladimir J. N. Bykov, Kenji M. Fujihara, Melodie Grandin, Xiaodun Li, Susanne Ramm, Corina Behrenbruch, Kaylene J. Simpson, Frederic Hollande, Lars Abrahmsen, Nicholas J. Clemons, Klas G. Wiman
Summary: The tumor suppressor gene TP53 is commonly mutated in cancer, and the compound APR-246 shows promise in targeting mutant p53 protein for cancer therapy. Combining APR-246 with inhibitors of efflux pump MRP1/ABCC1 results in synergistic tumor cell death, particularly in TP53 mutant cells, indicating the importance of redox homeostasis in response to mutant p53-targeted therapy.
EMBO MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Sabrina Blondy, Hugo Talbot, Sofiane Saada, Niki Christou, Serge Battu, Julie Pannequin, Marie-Odile Jauberteau, Fabrice Lalloue, Mireille Verdier, Muriel Mathonnet, Aurelie Perraud
Summary: Colorectal cancer (CRC) is the third most common cancer worldwide, and resistance to 5-fluorouracil (5-FU) can lead to poor clinical outcomes. Sortilin overexpression has been identified as a potential biomarker of 5-FU resistance in CRC, with higher levels correlating with tumor grades. This new prognostic factor may be used to combat CRC aggressiveness.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Medicine, General & Internal
Maarten J. IJzerman, Jasper de Boer, Arun Azad, Koen Degeling, Joel Geoghegan, Chelsee Hewitt, Frederic Hollande, Belinda Lee, Yat Ho To, Richard W. Tothill, Gavin Wright, Jeanne Tie, Sarah-Jane Dawson
Summary: Blood-based liquid biopsies are emerging as a new and promising diagnostic and monitoring tool for cancer, with potential for better clinical utility but at a higher cost. Routine implementation will require defining clinical utility, minimum quality standards, and testing platform performance to integrate them into current clinical workflows effectively.
Article
Oncology
J. Giraud, M. Foroutan, J. Boubaker-Vitre, F. Grillet, Z. Homayed, U. Jadhav, P. Crespy, C. Breuker, J-F. Bourgaux, J. Hazerbroucq, C. Pignodel, B. Brulin, R. A. Shivdasani, P. Jay, F. Hollande, J. Pannequin
Summary: Progastrin, produced by a subset of endocrine cells in intestinal crypts, transitions to being mainly sourced from intestinal stem cells in early adenomas, suggesting a feed-forward mechanism between progastrin and Wnt signaling as a hallmark of early neoplastic transformation in mouse and human colonic adenomas.
TRANSLATIONAL ONCOLOGY
(2021)
Review
Medicine, Research & Experimental
Morgan Brisset, Melodie Grandin, Agnes Bernet, Patrick Mehlen, Frederic Hollande
Summary: Dependence receptors play a role in promoting cell survival and positive signaling when activated, but trigger apoptosis when unbound to their ligand. Their abnormal regulation in cancer cells allows them to evade apoptosis and promote tumorigenesis, making them potential targets for therapy.
EMBO MOLECULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Jean Berthelet, Verena C. Wimmer, Holly J. Whitfield, Antonin Serrano, Thomas Boudier, Stefano Mangiola, Michal Merdas, Farrah El-Saafin, David Baloyan, Jordan Wilcox, Steven Wilcox, Adam C. Parslow, Anthony T. Papenfuss, Belinda Yeo, Matthias Ernst, Bhupinder Pal, Robin L. Anderson, Melissa J. Davis, Kelly L. Rogers, Frederic Hollande, Delphine Merino
Summary: The study optimized the use of optical barcoding to visualize and characterize cancer subclones, revealing that lung metastases were highly polyclonal while liver metastases were not. The transcriptome of the subclones varied according to their metastatic niche, indicating that the heterogeneity observed in metastases is largely dictated by the tumor microenvironment.
Review
Biochemistry & Molecular Biology
Laureline Roger, Fanny Tomas, Veronique Gire
Summary: Cellular senescence is an essentially irreversible proliferative arrest state where cells release pro-inflammatory and proteolytic factors. Different types of senescent cells accumulate in various tissues and organs with unique functions. Understanding how cells undergo extensive changes to induce a common cellular state is crucial for cancer prevention and improving health during aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Carolyn Shembrey, Jai Smith, Melodie Grandin, Nathalia Williams, Hyun-Jung Cho, Christina Molck, Corina Behrenbruch, Benjamin N. J. Thomson, Alexander G. Heriot, Delphine Merino, Frederic Hollande
Summary: Colorectal cancer is the second most common cancer worldwide, and tumor heterogeneity affects treatment outcome. This study presents a multi-color barcoding method for real-time quantification of tumor heterogeneity. The results show limited heterogeneity in traditional cell culture models and better representation of heterogeneity in patient-derived organoids. The optimized imaging protocol allows for real-time tracking of clonal dynamics. In summary, this technique is of great importance for monitoring tumor heterogeneity in cell culture models.
Article
Oncology
Guillaume Belthier, Zeinab Homayed, Fanny Grillet, Christophe Duperray, Julie Vendrell, Ilona Krol, Sophie Bravo, Jean-Christophe Boyer, Olivia Villeronce, Jihane Vitre-Boubaker, Diana Heaug-Wane, Francoise Macari-Fine, Jai Smith, Matthieu Merlot, Gerald Lossaint, Thibault Mazard, Fabienne Portales, Jerome Solassol, Marc Ychou, Nicola Aceto, Emilie Mamessier, Francois Bertucci, Jean Marc Pascussi, Emmanuelle Samalin, Frederic Hollande, Julie Pannequin
Summary: This study demonstrated the potential of using CD44v6 as a complementary marker to detect and purify CTCs from colorectal cancer and breast cancer patients. CD44v6 is not limited to colorectal cancer, indicating its usefulness in detecting CTCs from cancers of different origins. The results suggest the need for more efficacious combined markers to encompass CTC heterogeneity.
Review
Oncology
Ronnie Ren Jie Low, Wei Wen Lim, Paul M. Nguyen, Belinda Lee, Michael Christie, Antony W. Burgess, Peter Gibbs, Sean M. Grimmond, Frederic Hollande, Tracy L. Putoczki
Summary: PDO biobanks derived from PDAC patients have provided opportunities for developing new therapies and advancing our understanding of PDAC biology. Developing coculturing techniques for specific immune or stromal cell populations will help improve our understanding of the tumor microenvironment and its impact on treatment response.
Article
Cell Biology
Gerald Lossaint, Andela Horvat, Veronique Gire, Katarina Bacevic, Karim Mrouj, Fabienne Charrier-Savournin, Virginie Georget, Daniel Fisher, Vjekoslav Dulic
Summary: The balance between p21 and Chk1 controls cyclin D-CDK activity during cell cycle arrest, with p21 activating RB in non-transformed cells and Chk1 maintaining its activity in cancer cells. The compromised G2 exit in cancer cells is associated with sustained Chk1 activity, delayed p21 induction, untimely cyclin E1 re-expression, and genome reduplication.
JOURNAL OF CELL SCIENCE
(2022)
Article
Cell & Tissue Engineering
Lucile Bansard, Oceane Bouvet, Elisa Moutin, Gaetan Le Gall, Alessandro Giammona, Elodie Pothin, Marion Bacou, Cedric Hassen-Khodja, Benoit Bordignon, Jean Francois Bourgaux, Michel Prudhomme, Frederic Hollande, Julie Pannequin, Jean Marc Pascussi, Chris Planque
Summary: This study identifies miR-148a as a targetable element upstream of PXR signaling in CSCs, which can decrease PXR expression and impair tumor relapse after chemotherapy. The anti-helminthic drug niclosamide is identified as an inducer of miR-148a expression, which decreases PXR expression and CSC numbers, and synergizes with chemotherapeutic agents to prevent tumor relapse.
Article
Oncology
Jean Berthelet, Momeneh Foroutan, Dharmesh D. Bhuva, Holly J. Whitfield, Farrah El-Saafin, Joseph Cursons, Antonin Serrano, Michal Merdas, Elgene Lim, Emmanuelle Charafe-Jauffret, Christophe Ginestier, Matthias Ernst, Frederic Hollande, Robin L. Anderson, Bhupinder Pal, Belinda Yeo, Melissa J. Davis, Delphine Merino
Summary: This study identified gene partners associated with sensitivity or resistance to anti-cancer therapies and used predictive models to estimate drug responses in patient samples. It discovered a new gene signature to identify breast cancer tumors likely to respond to cisplatin in the absence of BRCA1 mutations. This research is an important advance in applying platinum-based therapies in non-routine patient groups and guiding drug choice based on tumor molecular profiles.
Article
Biochemistry & Molecular Biology
Alawiya Reslan, Juliano G. Haddad, Philippe Despres, Jean-Loup Bascands, Gilles Gadea
Summary: High glucose levels inhibit the replication of Zika virus and stimulate the production of antiviral substances in cells. Viperin is one of these substances and it inhibits Zika virus growth by stimulating the production of IFN-beta.