Journal
REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
Volume 21, Issue 3, Pages 304-307Publisher
BRAZILIAN COLL VETERINARY PARASITOLOGY
DOI: 10.1590/S1984-29612012000300024
Keywords
Kala-azar; visceral leishmaniasis; molecular diagnosis; 18S rRNA gene; L. infantum chagasi
Categories
Ask authors/readers for more resources
The objective of this study was to design and evaluate new primers for species-specific detection of L. infantum chagasi using PCR. Two combinations of primer pairs were established with the aim of obtaining specific amplification products from the L. infantum chagasi 18S rRNA gene. The combinations of the primer pairs and the respective sizes of the PCR products, based on the U422465 GenBank reference sequence of L. infantum chagasi, were: LCS1/LCS3 (259 bp) and LCS2/LCS3 (820 bp). It was concluded that the new PCR assays optimized using the primer pairs LCS1/LCS3 and LCS2/LCS3 were effective for specific detection of L. infantum chagasi, with analytical sensitivity to detect 1 pg/mu L of DNA.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available