Article
Multidisciplinary Sciences
Sam Buckberry, Xiaodong Liu, Daniel Poppe, Jia Ping Tan, Guizhi Sun, Joseph Chen, Trung Viet Nguyen, Alex de Mendoza, Jahnvi Pflueger, Thomas Frazer, Dulce B. Vargas-Landin, Jacob M. Paynter, Nathan Smits, Ning Liu, John F. Ouyang, Fernando J. Rossello, Hun S. Chy, Owen J. L. Rackham, Andrew L. Laslett, James Breen, Geoffrey J. Faulkner, Christian M. Nefzger, Jose M. Polo, Ryan Lister
Summary: Cells undergo a significant epigenome reconfiguration during reprogramming to hiPS cells. The differences in epigenomes between hiPS cells and hES cells affect the function of hiPS cells. Researchers characterized the emergence and persistence of these epigenetic differences and developed a TNT reprogramming strategy that corrects epigenetic memory and aberrations, making hiPS cells more similar to hES cells at a molecular and functional level.
Article
Cell & Tissue Engineering
Chuti Laowtammathron, Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Pimonwan Srisook, Phatchanat Klaihmon, Pakpoom Kheolamai, Sudjit Luanpitpong, Surapol Issaragrisil
Summary: The study explores the role of YAP in hematopoietic differentiation of hiPSCs. It is found that suppressing YAP activity enhances the efficiency of hematopoietic differentiation and increases the yield of HSPCs.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Biochemistry & Molecular Biology
Agnieszka Fus-Kujawa, Barbara Mendrek, Anna Trybus, Karolina Bajdak-Rusinek, Karolina L. Stepien, Aleksander L. Sieron
Summary: Induced pluripotent stem cells (iPSCs) show potential in gene therapy and disease modeling. To safely apply this technology in clinical settings, optimal reprogramming factors and vectors must be considered to reduce the risk of insertional tumorigenesis.
Article
Genetics & Heredity
Niccolo Liorni, Alessandro Napoli, Stefano Castellana, Sebastiano Giallongo, Daniela Rehakova, Oriana Lo Re, Irena Koutna, Tommaso Mazza, Manlio Vinciguerra
Summary: This study used a combination of CUT&Tag and RNA-Seq data to analyze the differences between macroH2A1.1 and macroH2A1.2 during iPSCs reprogramming. The results demonstrated that macroH2A1.1 had wider genome occupancy and specifically activated ectoderm/neural processes during reprogramming.
Article
Multidisciplinary Sciences
Karlijn A. L. Hasaart, Freek Manders, Joske Ubels, Mark Verheul, Markus J. van Roosmalen, Niels M. Groenen, Rurika Oka, Ewart Kuijk, Susana M. Chuva de Sousa Lopes, Ruben van Boxtel
Summary: This study compares the mutation burden between cultured iPSCs and their isogenic embryonic cells, finding that iPSCs maintain genomic integrity during culture at a similar degree as pluripotent cells in vivo.
Article
Cell & Tissue Engineering
Shijia Liuyang, Guan Wang, Yanglu Wang, Huanjing He, Yulin Lyu, Lin Cheng, Zhihan Yang, Jingyang Guan, Yao Fu, Jialiang Zhu, Xinxing Zhong, Shicheng Sun, Cheng Li, Jinlin Wang, Hongkui Deng
Summary: We have developed a robust and chemically defined reprogramming protocol that significantly shortens the induction time for generating human pluripotent stem cells from somatic cells. This protocol promotes cell proliferation and metabolic activities, leading to a more direct reprogramming process.
Article
Biophysics
M. R. Poorna, S. Sudhindran, M. Thampi, Ullas Mony
Summary: The study demonstrated that cellulose nanofibril gel can efficiently differentiate human induced pluripotent stem cells into hepatocyte-like cells. The differentiated HLCs from both CNF and matrigel substrates expressed specific hepatocyte markers and exhibited functional maturity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Fang Liu, Jing Wang, Yongli Yue, Chen Li, Xuemin Zhang, Jinzhu Xiang, Hanning Wang, Xueling Li
Summary: This study successfully used LCDM to generate giPSCs from goat fetal fibroblasts, demonstrating their stem cell morphology and pluripotency. This achievement provides a new approach for generating pluripotent stem cells from domestic animals and lays the foundation for basic and applied research in biology and agriculture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Sebastiano Giallongo, Daniela Rehakova, Marco Raffaele, Oriana Lo Re, Irena Koutna, Manlio Vinciguerra
Summary: Induced pluripotent stem cells (iPSCs) have great potential in regenerative medicine and therapy, but concerns arise regarding genomic instability due to high oxidative stress. Future research should focus on the impact of redox balance and epigenetic modifications on iPSCs.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Article
Biochemistry & Molecular Biology
Sergey A. Sinenko, Andrey A. Kuzmin, Elena V. Skvortsova, Sergey V. Ponomartsev, Evgeniya V. Efimova, Michael Bader, Natalia Alenina, Alexey N. Tomilin
Summary: In this study, the researchers investigated the impact of endogenous serotonin on reprogramming to pluripotency and found that serotonin synthesis has a negative effect on somatic cell reprogramming.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Hyun Kyu Kim, Tae Won Ha, Man Ryul Lee
Summary: Cells are the basic units of organisms, with over 30 trillion cells generated from a single-cell fertilized egg through division and differentiation. Establishing the Human Cell Atlas aims to understand the mechanisms underlying diseases and cellular activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Shuai Yu, Rui Zhang, Qiaoyan Shen, Zhenshuo Zhu, Juqing Zhang, Xiaolong Wu, Wenxu Zhao, Na Li, Fan Yang, Hongjiang Wei, Jinlian Hua
Summary: By overexpressing the transcription factor ESRRB, flat, monolayered piPSCs were created with enhanced chimeric capacity into the trophectoderm. ESRRB directly regulates the expressions of trophoblast stem cell markers, and its cooperation with OCT4 facilitates the transition from pluripotent state to trophoblast-like state.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yue Su, Ling Wang, Zhiqiang Fan, Ying Liu, Jiaqi Zhu, Deborah Kaback, Julia Oudiz, Tayler Patrick, Siu Pok Yee, Xiuchun (Cindy) Tian, Irina Polejaeva, Young Tang
Summary: This study successfully generated biPSCs with long-term self-renewal capacity and pluripotent characteristics from bovine mesenchymal stem cells. The cells exhibited morphological changes under different culture conditions, and transcriptome analysis showed high similarity to bovine ESCs and embryos. The naive-like biPSCs also demonstrated the ability to integrate into extra-embryonic tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Rachel Lasry, Noam Maoz, Albert W. Cheng, Nataly Yom Tov, Elisabeth Kulenkampff, Meir Azagury, Hui Yang, Cora Ople, Styliani Markoulaki, Dina A. Faddah, Kirill Makedonski, Dana Orzech, Ofra Sabag, Rudolf Jaenisch, Yosef Buganim
Summary: Elimination of one allele of two pluripotency genes in pluripotent cells leads to a significant decrease in pluripotency induction ability and abnormal gene methylation.
Article
Biology
Wenyu Ding, Changjiang Zhang, Baisong Wang, Xin Zhou, Le Sun, Suijuan Zhong, Jing Liu, Junjing Zhang, Xiaoqun Wang, Qian Wu
Summary: The study reveals the crucial roles of the centrosomal protein Cenpj in neurogenesis, cerebral cortex development, and body weight regulation. Deletion of Cenpj leads to obesity in mice and affects hypothalamic cell proliferation, neuron number, and function, providing insights into the pathological mechanisms of related diseases.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xianwen Ren, Wen Wen, Xiaoying Fan, Wenhong Hou, Bin Su, Pengfei Cai, Jiesheng Li, Yang Liu, Fei Tang, Fan Zhang, Yu Yang, Jiangping He, Wenji Ma, Jingjing He, Pingping Wang, Qiqi Cao, Fangjin Chen, Yuqing Chen, Xuelian Cheng, Guohong Deng, Xilong Deng, Wenyu Ding, Yingmei Feng, Rui Gan, Chuang Guo, Weiqiang Guo, Shuai He, Chen Jiang, Juanran Liang, Yi-min Li, Jun Lin, Yun Ling, Haofei Liu, Jianwei Liu, Nianping Liu, Shu-Qiang Liu, Meng Luo, Qiang Ma, Qibing Song, Wujianan Sun, GaoXiang Wang, Feng Wang, Ying Wang, Xiaofeng Wen, Qian Wu, Gang Xu, Xiaowei Xie, Xinxin Xiong, Xudong Xing, Hao Xu, Chonghai Yin, Dongdong Yu, Kezhuo Yu, Jin Yuan, Biao Zhang, Peipei Zhang, Tong Zhang, Jincun Zhao, Peidong Zhao, Jianfeng Zhou, Wei Zhou, Sujuan Zhong, Xiaosong Zhong, Shuye Zhang, Lin Zhu, Ping Zhu, Bin Zou, Jiahua Zou, Zengtao Zuo, Fan Bai, Xi Huang, Penghui Zhou, Qinghua Jiang, Zhiwei Huang, Jin-Xin Bei, Lai Wei, Xiu-Wu Bian, Xindong Liu, Tao Cheng, Xiangpan Li, Pingsen Zhao, Fu-Sheng Wang, Hongyang Wang, Bing Su, Zheng Zhang, Kun Qu, Xiaoqun Wang, Jiekai Chen, Ronghua Jin, Zemin Zhang
Summary: The study revealed the presence of immune response dysfunction in COVID-19 patients, with different peripheral immune subtype changes associated with clinical features such as age, sex, severity, and disease stages. Additionally, dramatic transcriptomic changes were observed within virus-infected cells, and upregulation of S100A8/A9 in peripheral blood may contribute to the cytokine storms frequently observed in severe patients.
Article
Neurosciences
Yingchao Shi, Qian Wu, Xiaoqun Wang
Summary: Understanding human brain development is crucial in neurobiology, but limited accessibility to human brain tissues has led to reliance on rodent studies. The advancement of brain organoid technology offers promising potential in decoding human brain development and uncovering brain disease pathology.
CURRENT OPINION IN NEUROBIOLOGY
(2021)
Article
Biology
Zhiyong Xie, Mengdi Wang, Zeyuan Liu, Congping Shang, Changjiang Zhang, Le Sun, Huating Gu, Gengxin Ran, Qing Pei, Qiang Ma, Meizhu Huang, Junjing Zhang, Rui Lin, Youtong Zhou, Jiyao Zhang, Miao Zhao, Minmin Luo, Qian Wu, Peng Cao, Xiaoqun Wang
Summary: The research reveals that neurons in the superior colliculus play distinct roles in sensorimotor transformation, with different functions defined by the expression of specific marker genes. These neuronal subtypes independently mediate predator avoidance and prey capture behaviors through forming different input and output connections with other brain areas.
Article
Cell & Tissue Engineering
Xin Zhou, Yufeng Lu, Fangqi Zhao, Ji Dong, Wenji Ma, Suijuan Zhong, Mengdi Wang, Bosong Wang, Yuqing Zhao, Yingchao Shi, Qiang Ma, Tian Lu, Jun Zhang, Xiaoqun Wang, Qian Wu
Summary: This study reveals the cell-type and spatiotemporal characteristics of human hypothalamus development and finds that different cell fates are produced sequentially with spatial developmental asynchrony. Human hypothalamic gliogenesis occurs at an earlier stage compared to mice and shows different transcription profiles. Early oligodendrocyte cells in humans interact with neuronal cells through Wnt, Hippo, and integrin signals to regulate neuronal maturation.
Article
Multidisciplinary Sciences
Yingchao Shi, Mengdi Wang, Da Mi, Tian Lu, Bosong Wang, Hao Dong, Suijuan Zhong, Youqiao Chen, Le Sun, Xin Zhou, Qiang Ma, Zeyuan Liu, Wei Wang, Junjing Zhang, Qian Wu, Oscar Marin, Xiaoqun Wang
Summary: Genetic variation contributes to susceptibility to neurodevelopmental disorders by impacting the development of specific cell types, such as cortical and striatal GABAergic neurons. Using single-cell RNA sequencing, this study characterized the diversity of cell emergence in the human ganglionic eminences, where these neurons are generated. The findings demonstrate a regulatory logic controlling the specification, migration, and differentiation of GABAergic neurons in the human telencephalon that is evolutionarily conserved.
Article
Multidisciplinary Sciences
Qianqian Zhang, Sijin Cheng, Yongzhi Wang, Mengdi Wang, Yufeng Lu, Zengqi Wen, Yuxin Ge, Qiang Ma, Youqiao Chen, Yaowu Zhang, Ren Cao, Min Li, Weihao Liu, Bo Wang, Qian Wu, Wenqing Jia, Xiaoqun Wang
Summary: Spinal ependymomas, the most common spinal cord tumors in adults, exhibit intratumoral cellular heterogeneity with distinct functional phenotypes of tumor-associated macrophage (TAM) subsets. By combining single-cell RNA and ATAC sequencing data analysis, TEAD1 and EGR3 are identified as key regulators of TAM functional diversity. Stromal cells are found to mediate TAM diversity, suggesting a dual role of TAMs in tumor progression.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Xuelong Yao, Zongyang Lu, Zhanying Feng, Lei Gao, Xin Zhou, Min Li, Suijuan Zhong, Qian Wu, Zhenbo Liu, Haofeng Zhang, Zeyuan Liu, Lizhi Yi, Tao Zhou, Xudong Zhao, Jun Zhang, Yong Wang, Xingxu Huang, Xiaoqun Wang, Jiang Liu
Summary: This study investigates the differences in the epigenetic regulation of brain development between primates and identifies regulatory elements that may be related to cognitive capacity.
NATURE COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Bo Zeng, Zeyuan Lu, Yufeng Lu, Suijuan Zhong, Shenyue Qin, Luwei Huang, Yang Zeng, Zixiao Li, Hao Dong, Yingchao Shi, Jialei Yang, Yalun Dai, Qiang Ma, Le Sun, Lihong Bian, Dan Han, Youqiao Chen, Xin Qiu, Wei Wang, Oscar Marin, Qian Wu, Yongjun Wang, Xiaoqun Wang
Summary: This study analyzed over 400,000 cells from 14 human samples to understand the molecular and cellular landscape of early gastrulation and nervous system development. The researchers described the diversification of cell types, spatial patterning of neural tube cells, and the signaling pathways involved in the transformation of cells during early development. They also identified differentiation trajectories for different types of neurons and compared the features between human and mice embryos. This comprehensive atlas provides insights into the molecular mechanisms underlying gastrulation and early human brain development.
Article
Chemistry, Physical
Qian Wu, Yan Zhuo, Xiaoqun Wang, Mengdi Wang, Liangchen Zhuo, Wenji Ma
Summary: Spatial transcriptomics captures cellular spatial organization and provides new insights into various biological contexts. However, its wide application is hindered by technical challenges and immature data analysis methods. The Allen Brain Atlas (ABA) provides spatial gene expression data, but the current portal is not user-friendly. We developed AllenDigger, a toolkit that collects and preprocesses ABA expression data, visualizes spatial gene distribution, characterizes brain heterogeneity, and registers single-cell transcriptomics data to anatomical brain regions with high accuracy using machine learning methods.
JOURNAL OF PHYSICAL CHEMISTRY A
(2023)
Article
Biology
Shiyang Chang, Peina Wang, Yingying Han, Qiang Ma, Zeyuan Liu, Suijuan Zhong, Yufeng Lu, Ruiguo Chen, Le Sun, Qian Wu, Guofen Gao, Xiaoqun Wang, Yan-Zhong Chang
Summary: This study reveals that low intracellular iron level significantly inhibits neurodifferentiation, while supplementing iron can restore normal neural differentiation.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Cell Biology
Bin Yu, Qianqian Zhang, Lin Lin, Xin Zhou, Wenji Ma, Shaonan Wen, Chunyue Li, Wei Wang, Qian Wu, Xiaoqun Wang, Xiao-Ming Li
Summary: By analyzing RNA sequencing data from over 200,000 neurons in humans, macaques, mice, and chickens, this study reveals the precise conservation and relative divergence of cellular architecture in the amygdala. It highlights the importance of cell types and gene expression in amygdala for species evolution and specific adaptations.
Article
Zoology
Qiang Ma, Wenji Ma, Tian-Zhang Song, Zhaobo Wu, Zeyuan Liu, Zhenxiang Hu, Jian-Bao Han, Ling Xu, Bo Zeng, Bosong Wang, Yinuo Sun, Dan-Dan Yu, Qian Wu, Yong-Gang Yao, Yong-Tang Zheng, Xiaoqun Wang
Summary: This study analyzed single-nucleus transcriptomes from multiple organs of SARS-CoV-2-infected rhesus macaques, revealing that the liver had the most significant global transcriptional alterations. Lung epithelial cells, especially AT2 and ciliated cells, showed significant impairment, while fibroblasts exhibited signs of fibrosis. The kidney dataset indicated a tropism of tubule cells to SARS-CoV-2 infection, leading to membranous nephropathy. Pathological changes were also observed in astrocytes and oligodendrocytes in the cerebral cortex. Overall, this study broadened our understanding of disease features and immune defects caused by SARS-CoV-2 infection.
ZOOLOGICAL RESEARCH
(2022)
Article
Cell Biology
Wei Wang, Mengdi Wang, Meng Yang, Bo Zeng, Wenying Qiu, Qiang Ma, Xiaoxi Jing, Qianqian Zhang, Bosong Wang, Chonghai Yin, Jiyao Zhang, Yuxin Ge, Yufeng Lu, Weizhi Ji, Qian Wu, Chao Ma, Xiaoqun Wang
Summary: This study investigates the cellular heterogeneity and molecular characteristics of the hippocampi in macaques and aged humans using droplet-based single-nucleus RNA sequencing (snRNA-seq). The findings reveal the dynamics of the neurogenic lineage and the diversity of astrocytes and microglia in the hippocampus. Primate-specific markers are identified and their functions are validated. Additionally, active astrocytes and microglia with proinflammatory responses are observed in aged samples, suggesting their contribution to the decrease and variability of adult hippocampal neurogenesis.
Article
Neurosciences
Tianyuan Lei, Xuhong Liao, Xiaodan Chen, Tengda Zhao, Yuehua Xu, Mingrui Xia, Jiaying Zhang, Yunman Xia, Xiaochen Sun, Yongbin Wei, Weiwei Men, Yanpei Wang, Mingming Hu, Gai Zhao, Bin Du, Siya Peng, Menglu Chen, Qian Wu, Shuping Tan, Jia-Hong Gao, Shaozheng Qin, Sha Tao, Qi Dong, Yong He
Summary: The study found that the modular structure of child brain networks changes over time, with higher network switching in the association cortex and less switching in primary regions. As development progresses, the brain networks exhibit progressive maturation with reduced modular dynamics, especially in transmodal and sensorimotor regions.
