4.7 Article

On the Interaction of Adherent Cells with Thermoresponsive Polymer Coatings

Journal

POLYMERS
Volume 6, Issue 4, Pages 1164-1177

Publisher

MDPI
DOI: 10.3390/polym6041164

Keywords

thermoresponsive polymer; RGD; cell detachment; polyethylene glycol

Funding

  1. interdisciplinary network of excellence Synthetic Bioactive Surfaces of the Fraunhofer Society
  2. Max Planck Society

Ask authors/readers for more resources

Thermoresponsive polymer coatings allow the control of adhesion of cells on synthetic substrates. In particular, decreasing the temperature below the lower critical solution temperature (LCST) of the polymer triggers the non-invasive detachment of cells from their cultivation substrate. Widening the range of applications of these coatings in cellular biotechnology requires a better understanding of their interaction with cells. By monitoring the morphological changes of cells during their detachment at various temperatures, we provide evidence that cell detachment is an active process. Analyses of cell residues that are left behind by the cells on the substrate during their detachment, further support this notion. In the second part of this work, we show that the kinetics of adhesion and the efficiency of detachment of cells can be controlled through the coadsorption of molecules bearing the peptide motif RGD (arginine-glycine-aspartic acid) with the polymers.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available