Article
Multidisciplinary Sciences
Charlotte A. James, Yuexin Xu, Melissa S. Aguilar, Lichen Jing, Erik D. Layton, Martine Gilleron, Adriaan J. Minnaard, Thomas J. Scriba, Cheryl L. Day, Edus H. Warren, David M. Koelle, Chetan Seshadri
Summary: This study reveals the importance of CD4 and CD8 co-receptors in recognizing mycobacterial glycolipid antigens. The expression of either CD4 or CD8 increases the avidity of T cell receptors for mycolipids, leading to a higher intensity of fluorescence and decreased secretion threshold for interferon-gamma. Furthermore, CD8+ T cells show greater cytotoxicity compared to CD4+ T cells in individuals with active tuberculosis.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Michiel van Gent, Werner J. D. Ouwendijk, Victoria L. Campbell, Kerry J. Laing, Georges M. G. M. Verjans, David M. Koelle
Summary: This study suggests that VZV-specific CD8 T-cells play a limited role in maintaining latent infection of varicella-zoster virus in human trigeminal ganglia.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Microbiology
Alexander Dowell, Tracey Haigh, Gordon Ryan, James Turner, Heather Long, Graham Taylor
Summary: Epstein Barr Virus (EBV) infects a large percentage of the population and establishes latent infection in B-cells. Efforts to develop EBV prophylactic vaccination have mainly focused on neutralising antibodies, but generating T-cell responses against EBV structural proteins could be a promising alternative strategy. Research has shown that EBV capsid proteins BcLF1, BDLF1, and BORF1 are frequent targets of T-cell responses, and CD4+ T-cells specific for these proteins can recognize and control newly EBV-infected cells, even in long-term viral carriage. This study highlights the potential of targeting structural antigens for prophylactic EBV vaccine development.
Article
Virology
Amanda Berube, Grzegorz B. Gmyrek, Derek J. Royer, Daniel J. J. Carr
Summary: TRIM21 deficiency does not affect ocular pathology caused by HSV-1 infection but significantly increases the amount of virus recovered from the trigeminal ganglia.
Article
Biology
Norimasa Iwanami, Malte Petersen, Dagmar Diekhoff, Thomas Boehm
Summary: T cell differentiation in the thymus results in a skewed CD4/CD8 ratio, with a greater number of CD4(+) cells. The use of high-resolution endogenous CRISPR/Cas9 barcoding reveals that larger clone size of CD4(+) cells and a higher likelihood of T cell receptor clonotypes for pMHCII interactions contribute to this imbalance.
COMMUNICATIONS BIOLOGY
(2022)
Article
Immunology
Spencer E. E. Brightman, Angelica Becker, Rukman R. R. Thota, Martin S. S. Naradikian, Leila Chihab, Karla Soria Zavala, Ryan Q. Q. Griswold, Joseph S. S. Dolina, Ezra E. W. Cohen, Aaron M. M. Miller, Bjoern Peters, Stephen P. P. Schoenberger
Summary: CD4(+) T cells have important roles in immune responses, either directly or through accessory cells such as CD8(+) T lymphocytes. While the role of NeoAg-specific CD8(+) T cells in cancer has been extensively studied, the role of NeoAg-specific CD4(+) T cells is less well understood.
Article
Immunology
Simona Mutascio, Talia Mota, Lavinia Franchitti, Ashish A. Sharma, Abigail Willemse, Sydney N. Bergstresser, Hong Wang, Maura Statzu, Gregory K. Tharp, Jared Weiler, Rafick-Pierre Sekaly, Steven E. Bosinger, Mirko Paiardini, Guido Silvestri, R. Brad Jones, Deanna A. Kulpa
Summary: Using a primary cell-based in vitro latency model, this study demonstrates that CD8+ T cells can suppress HIV expression and promote latency by inducing specific changes in metabolic and signaling pathways that increase CD4+ T cell survival and stemness. This discovery of CD8-specific mechanisms involved in pro-latency activity may contribute to the development of strategies to eliminate the viral reservoir in individuals with HIV.
Article
Immunology
Diahann T. S. L. Jansen, Monique T. A. de Beijer, Robbie J. Luijten, Kitty Kwappenberg, Anna-Sophia Wiekmeijer, Amy L. Kessler, Roel F. A. Pieterman, Rachid Bouzid, Willem-Jan Krebber, Robert A. de Man, Cornelis J. M. Melief, Sonja I. Buschow
Summary: This study designed a set of SLPs capable of inducing multifunctional T cell responses ex vivo, which provide important components for a novel therapeutic vaccine to cure cHBV.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Joey Ming Er Lim, Anthony Tanoto Tan, Nina Le Bert, Shou Kit Hang, Jenny Guek Hong Low, Antonio Bertoletti
Summary: Nasal-resident T cells specific for SARS-CoV-2 were detected in vaccinated individuals only after infection, highlighting the significance of nasal challenge in the formation of antiviral immunity at the site of infection.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Medicine, General & Internal
Tomabu Adjobimey, Julia Meyer, Vedrana Terkes, Marijo Parcina, Achim Hoerauf
Summary: The presence of helminth antigens in COVID-19 patients from Africa may contribute to the lower incidence of the disease in the continent. Helminth antigens reduce the immune response of helper T cells to SARS-CoV-2, while maintaining the cytotoxic T cell response.
Article
Oncology
Selma Bekri, Reunet Rodney-Sandy, Diana Gruenstein, Anna Mei, Bjarne Bogen, John Castle, Daniel Levey, Hearn Jay Cho
Summary: This study demonstrates that a vaccine targeting a tumor-specific neoantigen efficiently induces CD4 T cell-mediated tumor protection in a mouse model. The vaccine does not directly induce cytotoxic activity in CD4 T cells, but activation of CD8 CTL against tumor-associated antigens not present in the vaccine is a major mechanism of tumor immunity.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Virology
Yongzhong Duan, Le Sun, Qihan Li
Summary: This article provides a summary of the characteristics of HSV-1, including its latent infection in neurons and lytic infection in other cells, as well as the role of HSV-1-encoded miRNAs in the virus and host cell life cycle. The review primarily focuses on recent advances in understanding HSV-1-encoded miRNA expression, function, and mechanism.
Article
Biology
V. T. Khomich, N. Dyshliuk, T. A. Mazurkevych, S. Guralska, S. Usenko
Summary: The immune formations of birds' digestive organs have unique properties formed during embryonic development, involving lymphocyte clones responding to foreign antigens. Different subpopulations of lymphocytes were found in the esophageal tonsil and Meckel diverticulum, with higher levels of CD20+ lymphocytes in diffuse lymphoid tissue.
REGULATORY MECHANISMS IN BIOSYSTEMS
(2021)
Article
Biochemistry & Molecular Biology
R. K. Salyaev, N. Rekoslavskaya
Summary: The antigenic protein HPV6 L1 was synthesized in a plant expression system using transgenic tomato fruit for the development of an oral vaccine against anogenital papillomaviruses. New data on the induction of T-cell immune response in mice immunization experiments showed activation of interferon, CD4 and CD8 T lymphocytes, and granzyme B secretion in peripheral blood cells and splenocytes of vaccinated mice.
DOKLADY BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Virology
Georgios Katzilieris-Petras, Xin Lai, Ahmad S. Rashidi, Georges M. G. M. Verjans, Line S. Reinert, Soren R. Paludan
Summary: This study demonstrates the vital role of microglia in controlling the replication of HSV1 in the central nervous system. Depletion of microglia leads to increased viral titers and disease severity, highlighting their importance in the early antiviral response. The study also reveals the contribution of the immune sensor cGAS in the activity of microglia.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Charlotte A. James, Yuexin Xu, Melissa S. Aguilar, Lichen Jing, Erik D. Layton, Martine Gilleron, Adriaan J. Minnaard, Thomas J. Scriba, Cheryl L. Day, Edus H. Warren, David M. Koelle, Chetan Seshadri
Summary: This study reveals the importance of CD4 and CD8 co-receptors in recognizing mycobacterial glycolipid antigens. The expression of either CD4 or CD8 increases the avidity of T cell receptors for mycolipids, leading to a higher intensity of fluorescence and decreased secretion threshold for interferon-gamma. Furthermore, CD8+ T cells show greater cytotoxicity compared to CD4+ T cells in individuals with active tuberculosis.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Lichen Jing, Xia Wu, Maxwell P. Krist, Tien-Ying Hsiang, Victoria L. Campbell, Christopher L. McClurkan, Sydney M. Favors, Lawrence A. Hemingway, Charmie Godornes, Denise Q. Tong, Stacy Selke, Angela C. LeClair, Chu-Woo Pyo, Daniel E. Geraghty, Kerry J. Laing, Anna Wald, Michael Gale, David M. Koelle
Summary: SARS-CoV-2 elicits a strong T cell response, with CD8 and CD4 T cells from convalescent individuals recognizing infected cells and inactivated whole viral antigen respectively. CD8 T cells exhibit low specificity, while CD4 T cells show higher breadth. Additionally, some CD4 T cell lines enriched using SARS-CoV-2 can cross-recognize seasonal coronavirus antigens.
Article
Oncology
Evalyn E. A. P. Mulder, Jeffrey Damman, Danielle Verver, Astrid A. M. van der Veldt, Sam Tas, Tamana Khemai-Mehraban, Kim C. Heezen, Roxane A. Wouters, Cornelis Verhoef, Georges M. G. M. Verjans, Anton W. Langerak, Dirk J. Grunhagen, Antien L. Mooyaart
Summary: T-VEC treatment in melanoma patients leads to the presence of plasma cells in biopsy samples. An angiocentric granulomatous pattern is associated with a durable complete response. These findings suggest that plasma cells may play a role in the response mechanism.
Article
Immunology
Peter-Paul A. Unger, Anna E. Oja, Tamana Khemai-Mehraban, Werner J. D. Ouwendijk, Pleun Hombrink, Georges M. G. M. Verjans
Summary: This study revealed that the human trigeminal ganglia (TG) is an immunocompetent environment where both CD4 and CD8 tissue-resident memory T-cells (T-RM) are established and retained. These findings provide support for T-RM-targeted vaccine strategies.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Oncology
Candice Church, Thomas Pulliam, Natalie Longino, Song Y. Park, Kimberly S. Smythe, Vladimir Makarov, Nadeem Riaz, Lichen Jing, Robert Amezquita, Jean S. Campbell, Raphael Gottardo, Robert H. Pierce, Jaehyuk Choi, Timothy A. Chan, David M. Koelle, Paul Nghiem
Summary: This study identified and characterized tumor-specific Th1-skewed CD4 T cells targeting multiple neoantigens in a patient who experienced a profound and durable partial response to anti-PD-L1 therapy. This is the first report of neoantigen-specific T cell responses in MCC, suggesting that CD4 T cells may play an important role in the response to anti-PD-(L)1 therapy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Multidisciplinary Sciences
Kerry J. Laing, Werner J. D. Ouwendijk, Victoria L. Campbell, Christopher L. McClurkan, Shahin Mortazavi, Michael Elder Waters, Maxwell P. Krist, Richard Tu, Nhi Nguyen, Krithi Basu, Congrong Miao, D. Scott Schmid, Christine Johnston, Georges M. G. M. Verjans, David M. Koelle
Summary: This study reveals that varicella-zoster virus-specific T cells preferentially persist as tissue-resident-memory T cells in the rash-involved skin after recovery from zoster, indicating their importance in controlling skin infections.
NATURE COMMUNICATIONS
(2022)
Review
Virology
S. Reshwan K. Malahe, Jeroen J. A. van Kampen, Olivier C. C. Manintveld, Rogier A. S. Hoek, Caroline M. M. den Hoed, Carla C. C. Baan, Marcia M. L. Kho, Georges M. G. M. Verjans
Summary: Solid organ transplant recipients (SOTRs) face a high risk of human herpesvirus (HHV)-related morbidity and mortality due to immunosuppressive therapy. We aim to increase awareness of the HHV disease burden in SOTRs by providing an overview of current prevention and management strategies from the literature and guidelines. We discuss challenges in prevention, treatment, and future perspectives.
Article
Medicine, Research & Experimental
Daphne M. Hullegie-Peelen, Hector Tejeda Mora, Dennis A. Hesselink, Eric M. J. Bindels, Thierry P. P. van den Bosch, Marian C. Clahsen-van Groningen, Marjolein Dieterich, Sebastiaan Heidt, Robert C. Minnee, Georges M. G. M. Verjans, Martin J. Hoogduijn, Carla C. Baan
Summary: This study demonstrates the presence of diverse populations of tissue-resident lymphocytes (TRLs) in kidney transplants, including innate, innate-like, and adaptive TRLs. Chimerism of donor and recipient cells is common in kidney transplants and is present in all types of tissue-resident memory T cells. These cells may contribute to local protection against viral pathogens.
Meeting Abstract
Dermatology
Saumya Jani, Thomas H. Pulliam, Koshlan Mayer-Blackwell, Philip Bradley, David M. Koelle, Paul Nghiem
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Meeting Abstract
Immunology
Tara Reid, Charmie Godornes, Denise Tong, David M. Koelle
JOURNAL OF IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Andres Chang, Anton M. Sholukh, Andreas Wieland, David L. Jaye, Mary Carrington, Meei-Li Huang, Hong Xie, Keith R. Jerome, Pavitra Roychoudhury, Alexander L. Greninger, Jean L. Koff, Jonathon B. Cohen, David M. Koelle, Lawrence Corey, Christopher R. Flowers, Rafi Ahmed
Summary: Herpes simplex virus lymphadenitis (HSVL) is a rare presentation in patients with chronic lymphocytic leukemia (CLL), characterized by systemic symptoms and absence of herpetic lesions. The study found that HSV-specific immune responses during HSVL may contribute to decreased CLL tumor burden.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biotechnology & Applied Microbiology
Jason Z. Zhang, Hsien-Wei Yeh, Alexandra C. Walls, Basile I. M. Wicky, Kaitlin R. Sprouse, Laura A. VanBlargan, Rebecca Treger, Alfredo Quijano-Rubio, Minh N. Pham, John C. Kraft, Ian C. Haydon, Wei Yang, Michelle DeWitt, John E. Bowen, Cameron M. Chow, Lauren Carter, Rashmi Ravichandran, Mark H. Wener, Lance Stewart, David Veesler, Michael S. Diamond, Alexander L. Greninger, David M. Koelle, David Baker
Summary: The protein biosensor designed in this study utilizes thermodynamic coupling to detect neutralizing antibodies against SARS-CoV-2 variants, without the need for target modification, and can better distinguish differences between samples compared to traditional methods.
NATURE BIOTECHNOLOGY
(2022)
Meeting Abstract
Medicine, General & Internal
S. Wanner, V. L. Campbell, S. Selke, D. M. Koelle
JOURNAL OF INVESTIGATIVE MEDICINE
(2022)